- About
-
The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
Search results
Showing 1 to 2 of 2 (0.057 seconds)| 1 |
Inspiratory Off-Switch Mediated by Optogenetic Activation of Inhibitory Neurons in the preBötzinger Complex In VivoHülsmann, Swen, Hagos, Liya, Eulenburg, Volker, Hirrlinger, Johannes 02 February 2024 (has links)
The role of inhibitory neurons in the respiratory network is a matter of ongoing debate.
Conflicting and contradicting results are manifold and the question whether inhibitory neurons are
essential for the generation of the respiratory rhythm as such is controversial. Inhibitory neurons
are required in pulmonary reflexes for adapting the activity of the central respiratory network to the
status of the lung and it is hypothesized that glycinergic neurons mediate the inspiratory off-switch.
Over the years, optogenetic tools have been developed that allow for cell-specific activation of
subsets of neurons in vitro and in vivo. In this study, we aimed to identify the effect of activation
of inhibitory neurons in vivo. Here, we used a conditional transgenic mouse line that expresses
Channelrhodopsin 2 in inhibitory neurons. A 200 m multimode optical fiber ferrule was implanted
in adult mice using stereotaxic surgery, allowing us to stimulate inhibitory, respiratory neurons within
the core excitatory network in the preBötzinger complex of the ventrolateral medulla. We show that,
in anesthetized mice, activation of inhibitory neurons by blue light (470 nm) continuously or with
stimulation frequencies above 10 Hz results in a significant reduction of the respiratory rate, in some
cases leading to complete cessation of breathing. However, a lower stimulation frequency (4–5 Hz)
could induce a significant increase in the respiratory rate. This phenomenon can be explained by
the resetting of the respiratory cycle, since stimulation during inspiration shortened the associated
breath and thereby increased the respiratory rate, while stimulation during the expiratory interval
reduced the respiratory rate. Taken together, these results support the concept that activation of
inhibitory neurons mediates phase-switching by inhibiting excitatory rhythmogenic neurons in the
preBötzinger complex.
|
| 2 |
Modulatorische Effekte von Stickstoffmonoxid und Juvenilhormon auf die Kontrolle des Reproduktionsverhaltens in weiblichen Chorthippus biguttulus / Modulatory effects of nitric oxide and juvenile hormone on the control of reproductive behavior in female Chorthippus biguttulusWirmer, Andrea 01 July 2010 (has links)
No description available.
|
Page generated in 0.061 seconds