Currently the treatment of alcohol use disorder is very difficult and often requires the combination of therapy and medications, with many who undertake treatment experiencing relapse over time. There is also no treatment in use to prevent the development of alcohol use disorder. It is the aim of this work to provide information that may be useful for the development of a preventative treatment for developing alcohol use disorder by elucidating more of the acute effects of alcohol use. It is known that these effects originate in the brain. Within the brain are circuits made up of neurons that communicate with each other through chemical synapses. These chemical synapses involve the release of neurotransmitters from one neuron that are detected by another neuron, which initiates its own response. It is known that ethanol can change how much neurotransmitter is released from a neuron, depending on the specific neuron tested, and many researchers have implicated the "release machinery" as a target. It is also known that alcohol can affect lipid membrane properties that are important for the fusion of the vesicle membrane, encapsulating the neurotransmitter, with the cell membrane for release of the neurotransmitter outside of the neuron. It is not known if alcohol directly affects the SNARE proteins ("release machinery") or the lipid membranes to initiate the change in neurotransmitter release previously observed. Within this work you will find a discussion of the steps of neurotransmitter release and the known effects of anesthetics on components of this process, as an introduction to the topic (Chapters 1 and 2). In Chapters 3-5 you will find studies that successively dive deeper and deeper into the effects of alcohol on the SNARE proteins and lipid membranes. We show that ethanol is effective at a dose of 0.4% v/v or 64 mM at increasing fusion probability in a model of neurotransmitter release that uses the 3 SNARE proteins to drive fusion of a vesicle with a supported membrane. We also show that alcohol has little direct effect on the SNARE proteins themselves. In addition, we provide evidence that alcohol alters fusion oppositely, depending on which membrane leaflet it has most direct access to. In Chapter 5 we show that alcohol increases the probability of lipid tail protrusion in silico. Previously it has been shown that protrusion of one fatty acid tail of one lipid can initiate fusion of that membrane with an apposing membrane. These data provide further insight into the effects of alcohol on a neuron and we would argue are valuable to research pursuing treatment and prevention of alcohol use disorder.

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Botenstoffe, die Informationen zwischen Nervenzellen übertragen, werden Neurotransmitter genannt. Die Neurotransmitter Gamma-Aminobuttersäure, Glutamat und Serotonin sind Neurotransmitter des Zentralen Nervensystems. Sie beeinflussen die psychische Gesundheit, Emotionen und die Stimmungslage. Gamma-Aminobuttersäure ist der wichtigste Vertreter der hemmenden Neurotransmitter und sorgt für die Verminderung von Angst- und Spannungszuständen. Glutamat ist der häufigste exzitatorische Neurotransmitter. Serotonin ist entwicklungsgeschichtlich einer der ältesten Neurotransmitter und kommt auch in pflanzlichen Lebewesen vor. Beim Menschen sorgt er für eine positive Stimmungslage und hat eine anregende Wirkung. In dieser Arbeit sollen die Grundlagen der chemischen Signalübertragung dargestellt und die Wirkungsweise von Neurotransmittern aufgezeigt werden. Die Effekte der drei genannten Neurotransmitter an ihren spezifischen Rezeptoren werden näher erläutert, agonistisch und antagonistisch wirkende Substanzen werden aufgeführt. Am Beispiel der Benzodiazepine wird die Möglichkeit der Einflussnahme auf das Neurotransmittersystem der Gamma-Aminobuttersäure diskutiert. Ergänzt wird die schriftliche Ausarbeitung durch entsprechendes Bildmaterial. Das Bildmaterial soll als Vorlage für Animationen und graphische Darstellungen in dem noch zu entwickelnden interaktiven Lernprogramm Toxnetz-Explorer verwendet werden. Das Lernprogramm soll zukünftig für die Vermittlung von Lerninhalten im Rahmen des postgradualen Studiums „Toxikologie und Umweltschutz“ verwendet werden. Das Programm wird wichtige physiologische und organspezifische Aspekte graphisch und mittels Animationen darstellen und diese durch Fachtexte ergänzen. / Messenger substances that transmit information between neurons are called neurotransmit-ters. Gamma-aminobutyric acid, glutamate and serotonin are neurotransmitters of the central nervous system. They influence mental health, emotions and mood. Gamma-aminobutyric acid is the most important inhibitory neurotransmitters and is responsible for reducing anxiety and tension. Glutamate is the most common excitatory neurotransmitter. Serotonin is one of the oldest neurotransmitters in developmental history and is also found in plant organisms. In humans, it ensures a positive mood and has a stimulating effect. In this thesis, the basics of chemical signal transmission will be presented and the mode of action of neurotransmitters will be shown. The effects of the three neurotransmitters already mentioned at their specific receptors are explained in more detail, and agonistic and antago-nistic substances are listed. Using the example of benzodiazepines, the possibility of influ-encing the neurotransmitter system of gamma-aminobutyric acid is discussed. The written elaboration is supplemented by corresponding visual material. The visual material is to be used as a template for animations and graphic representations in the interactive learning pro-gramme Toxnetz-Explorer, which is developed actually. The learning programme will be be used in the future for the teaching of learning content of the postgraduate course 'Toxicology and Environmental Protection'. The programme will present important physiological and or-gan-specific aspects graphically and by means of animations and supplement these with specialist texts.

PTSD is a moderately heritable disorder that causes intense and chronic suffering in many afflicted individuals. The pathogenesis of PTSD is not well understood, and genetic mechanisms are particularly elusive. Neurotransmitter systems are thought to contribute to PTSD etiology and are the targets of most pharmacotherapies used to treat PTSD, including the only two FDA approved options and a wide array of off-label options. However, the degree to which variation in genes which encode for and regulate neurotransmitter receptors increase risk of developing PTSD is unclear. Recently, large collaborative groups of PTSD genetics researchers have completed genome-wide association studies (GWAS) using massive sample sizes and have made summary statistics available for public use. In 2018, a new technique for high-powered analysis of GWAS summary statistics called GSA-SNP2 was introduced. In order to explore the relationship between PTSD and genetic variants in widely theorized molecular targets, this study applied GSA-SNP2 to manually curated neurotransmitter receptor gene-sets. Curated gene-sets included nine total "neurotransmitter receptor group" gene-sets and 45 total "receptor subtype" gene-sets. Each "neurotransmitter receptor group" gene-sets was designed to capture concentration of genetic risk factors for PTSD within genes which encode for all receptor subtypes that are activated by a given neurotransmitter. In contrast, "receptor subtype" gene-sets focused on specific subtypes and also accounted for intracellular signaling; each was designed to capture concentration of genetic risk factors for PTSD within genes which encode for specific receptor subtypes and the intracellular signaling proteins through which they exert their effects. Due to practical considerations, this work used summary statistics derived from a GWAS with far fewer participants (2,424 cases; 7,113 controls) than initially planned (23,212 cases; 151,447 controls). Prior to controlling for multiple comparisons, 7 of the investigated gene-sets reached statistical significance at the p ≤ .05 level. However, after controlling for multiple comparisons, none of the investigated gene-sets reached statistical significance. Due to limited statistical power of the current work, these results should be interpreted very cautiously. The current study is best interpreted as a preliminary study and is most informative in relation to refining study design. Implications for next steps are emphasized in discussion and nominally significant results are synthesized with the literature to demonstrate the types of research questions that might be addressed by applying a refined version of this study design to a larger sample. / Doctor of Philosophy / Though nearly all individuals will be exposed to a potentially traumatic event in their lifetime, only a small percentage will experience PTSD, which is a severe psychological disorder. Though genetics are known contribute to an individual's level of risk for developing PTSD, relatively little is known about which particular genetic differences are key. Neurotransmitter receptors are thought to contribute to the risk for PTSD and are a key aspect of medications for PTSD. However, little is known about whether genetic differences in neurotransmitter receptors contribute to risk for developing PTSD. Recently, large collaborative groups of PTSD genetics researchers have completed studies which investigate genetic risk factors from across the genome using massive sample sizes and have made the statistical output of these studies available to the public. In 2018, a new technique called GSA-SNP2 was created to help assist with efforts to analyze aspects of that statistical output that have not been previously analyzed. This study used GSA-SNP2 to analyze the degree to which groups of neurotransmitter receptor genes contribute to the risk of developing PTSD. Due to the coronavirus pandemic, the researcher did not have access to the computing power needed to analyze the initially planned data which included 23,212 individuals with PTSD and 151,447 individuals without PTSD. As a substitute, the current work is an analysis using statistical output data from a study which included 2,424 individuals with PTSD and 7,113 individuals without PTSD. Based on a level of statistical significance that is typically used in most psychological studies, seven of the investigated gene-sets contribute highly to the risk for PTSD. However, it was necessary to use a different threshold for statistical significance due to the testing of many different groups of genes. After making that adjustment, none of the investigated gene-sets reached statistical significance. Due to limited statistical power of the current work, these results should be interpreted very cautiously. The current study is best interpreted as a preliminary study and is most informative in relation to refining study design. Implications for next steps are emphasized in discussion and nominally significant results are synthesized with the literature to demonstrate the types of research questions that might be addressed by applying a refined version of this study design to a larger sample.

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Ho, Tsz Wan. / Thesis (Ph.D.)--Chinese University of Hong Kong, 2009. / Includes bibliographical references (leaves 125-165). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Abstract also in Chinese.

<p>Communication between neuronal and non-neuronal is called volume transmission when the released neurotransmitter (NT) acts via diffusion and affects several target cells. Both the neurosecretory and postsynaptic cell responses are linked to [Ca²⁺]_i elevations. </p><p>In the present thesis the role of pre-and postsynaptic Ca²⁺ elevations has been investigated in the reconstituted "synapse" model comprised of NGF-differentiated PC12 and HEL cells as well as in SH-SY5Y neuroblastoma cells. In PC12 cells, both 70mM K⁺ and nicotine triggered NT release, which could be detected as a secondary [Ca²⁺]_i increase in surrounding HEL cells. Both secretagogues shared the same voltage-dependent Ca²⁺ influx pathway as judged from the pharmacological profile blockers of voltage-gated Ca²⁺ channels. The coupling of electrical responses to the activation of Ca²⁺ signaling via muscarinic receptors in SH-SY5Y cells was also studied. These data revealed that depolarization caused a considerable potentiation of the muscarinic Ca²⁺ response. The potentiated Ca²⁺ increase was mainly dependent on the enhanced Ca²⁺ influx and to a lesser extent on [Ca²⁺]_i release from intracellular stores. A phospholipase C (PLC) activator, m-3M3FBS was used to further study the role of G-protein coupled receptor (GPCR)-coupled Ca²⁺ signaling. However, it was found that m-3M3FBS instead triggered [Ca²⁺]_i elevations independently of PLC activation. </p><p>In conclusion, the results indicate that the magnitude of NT release from PC12 cells is sufficient to cause a robust activation of neighboring target cells. Postsynaptic muscarinic signaling is amplified due to integration of electrical excitation and GPCR signaling. The PLC activator, m-3M3FBS is not suitable for studies of PLC-mediated signals in intact cells.</p>

Communication between neuronal and non-neuronal is called volume transmission when the released neurotransmitter (NT) acts via diffusion and affects several target cells. Both the neurosecretory and postsynaptic cell responses are linked to [Ca2+]i elevations. In the present thesis the role of pre-and postsynaptic Ca2+ elevations has been investigated in the reconstituted "synapse" model comprised of NGF-differentiated PC12 and HEL cells as well as in SH-SY5Y neuroblastoma cells. In PC12 cells, both 70mM K+ and nicotine triggered NT release, which could be detected as a secondary [Ca2+]i increase in surrounding HEL cells. Both secretagogues shared the same voltage-dependent Ca2+ influx pathway as judged from the pharmacological profile blockers of voltage-gated Ca2+ channels. The coupling of electrical responses to the activation of Ca2+ signaling via muscarinic receptors in SH-SY5Y cells was also studied. These data revealed that depolarization caused a considerable potentiation of the muscarinic Ca2+ response. The potentiated Ca2+ increase was mainly dependent on the enhanced Ca2+ influx and to a lesser extent on [Ca2+]i release from intracellular stores. A phospholipase C (PLC) activator, m-3M3FBS was used to further study the role of G-protein coupled receptor (GPCR)-coupled Ca2+ signaling. However, it was found that m-3M3FBS instead triggered [Ca2+]i elevations independently of PLC activation. In conclusion, the results indicate that the magnitude of NT release from PC12 cells is sufficient to cause a robust activation of neighboring target cells. Postsynaptic muscarinic signaling is amplified due to integration of electrical excitation and GPCR signaling. The PLC activator, m-3M3FBS is not suitable for studies of PLC-mediated signals in intact cells.

The purpose of this dissertation was to gain insight into spinal sensory regulation during locomotion. To this end, I developed a novel in vitro spinal cord-hindlimb preparation (SCHP) composed of the isolated in vitro neonatal rat spinal cord oriented dorsal-up with intact hindlimbs locomoting on a custom-built treadmill or instrumented force platforms. The SCHP combines the neural and pharmacological accessibility of classic in vitro spinal cord preparations with intact sensory feedback from physiological hindlimb movements. thereby expanding our ability to study spinal sensory function. I then validated the efficacy of the SCHP for studying behaviorally-relevant, sensory-modulated locomotion by showing the impact of sensory feedback on in vitro locomotion. When locomotion was activated by serotonin and N-methyl D-aspartate, the SCHP produced kinematics and muscle activation patterns similar to the intact rat. The mechanosensory environment could significantly alter SCHP kinematics and muscle activitation patterns, showing that sensory feedback regulates in vitro spinal function. I further demonstrated that sensory feedback could reinforce or initiate SCHP locomotion. Using the SCHP custom-designed force platform system, I then investigated how presynaptic inhibition dynamically regulates sensory feedback during locomotion and how hindlimb mechanics influence this regulation. I hypothesized that contralateral limb mechanics would modulate presynaptic inhibition on the ipsilateral limb. My results indicate that contralateral limb stance-phase loading regulates ipsilateral swing-phase sensory inflow. As contralateral stance-phase force increases, contralateral afferents act via a GABAergic pathway to increase ipsilateral presynaptic inhibition, thereby inhibiting sensory feedback entering the spinal cord. Such force-sensitive contralateral presynaptic inhibition may help preserve swing, coordinate the limbs during locomotion, and adjust the sensorimotor strategy for task-specific demands. This work has important implications for sensorimotor rehabilitation. After spinal cord injury, sensory feedback is one of the few remaining inputs available for accessing spinal locomotor circuitry. Therefore, understanding how sensory feedback regulates and reinforces spinally-generated locomotion is vital for designing effective rehabilitation strategies. Further, sensory regulation is degraded by many neural insults, including spinal cord injury, Parkinson's disease, and stroke, resulting in spasticity and impaired locomotor function. This work suggests that contralateral limb loading may be an important variable for restoring appropriate sensory regulation during locomotion.