Thesis (M.S.)--Ohio University, June, 2009. / Title from PDF t.p. Includes bibliographical references.

Thesis (M.S.)--West Virginia University, 2005. / Title from document title page. Document formatted into pages; contains xvi, 145 p. : ill. (some col.), maps (some col.). Vita. Includes abstract. Includes bibliographical references.

The early Pliocene age Gray Fossil Site (GFS) was relatively recently found, with much still to be discovered and examined, and represents one of only a few sites of its age in eastern North America. It has been noted that the diverse faunal remains found at GFS are unique compared to what have been found at other fossil sites in North America from the same time period. Studying mammalian community structures at fossil sites can provide an abundance of information about the past environment and species adaptations to it, such as niche occupation of species, resource partitioning, and interactions between organisms and their environment. The main questions being asked in this study are: 1) what is the mammal community structure like at GFS?, and 2) how does the community structure at GFS compare to other contemporaneous sites? While studies of the fauna and flora have helped us to understand the ecosystem at GFS, detailed study of the mammal community will help us better understand this unique site. It is expected that the community structure and niche occupation of the mammals at GFS will be different from other contemporaneous sites, dominated by mammals adapted for life in the warm oak, hickory forest present at the site. A taxon-free approach to analysis will be used so that environments not sharing the same taxa or of different ages can be compared to one another. Each mammal species from GFS (48 total) and five well-known contemporaneous sites are categorized by body size, locomotor mode, and dietary preference to characterize the niches occupied by each species. Categorizations for each species will come from published works and measurements / ecomorphological analysis of specimens. Preliminary results show that GFS is different from other sites in that there are more brachyodont and tree dwelling/climbing adapted species present, and there are fewer running species present. The initial findings are likely associated with the site being a closed, forested ecosystem, compared to the more open environments of the other sites. Moving forward, descriptions pertaining to specifically how GFS and its mammal community structure compare to the niche occupation of similar species at other sites will be discussed. This project will also examine what more this analysis can reveal about the ecosystem at GFS, particularly how the herbivorous mammals can provide greater insight into what environmental conditions were like, including what vegetation was predominant at GFS.

The early Pliocene age Gray Fossil Site (GFS) is a fossil site that preserves diverse fauna and flora and represents one of only a few sites of its age in eastern North America. The assemblage of fauna found at GFS is unique compared to what has been found at other contemporaneous fossil sites across the continent. While studies of the fauna and flora have helped us to understand the ecosystem at GFS, detailed study of the diverse mammal community will help us better understand this unique site. Studying mammalian community structures at fossil sites can provide an abundance of information about the past such as, ecological inferences and climate conditions. To understand the paleoenvironment at GFS, I have gathered data from twenty modern communities and five well-known contemporaneous sites. Mammal species from each of these 26 sites have been categorized by body size, locomotor mode, and cheek tooth crown height to characterize the niches occupied by each species. Proportions of species within categories have been compared and analyzed across modern and fossil sites using discriminant function analysis (DFA). Results of the DFA demonstrate that modern communities with different climates and habitats can be distinguished based on the body size, locomotion, and cheek tooth crown heights of mammals living there. Moreover, functions can be used to infer conditions of past sites. Species diversity within categories was also compared across all sites. Results show that GFS has more brachydont and scansorial species present, and fewer cursorial species than occur at other contemporaneous sites. The results of the discriminant functional analysis indicate that the GFS community structure is most similar to modern temperate forest regions of the Himalayan Mountains in China and modern Washington Co., TN. Proportions of brachydont, gliding, and scansorial species appear to be driving variables behind these results.

No description available.

Eastern wild turkeys (Meleagris gallapavo silvestris) were extirpated from most of the Mississippi Alluvial Valley (MAV) by 1980 by overharvest and habitat loss. Federal programs have restored areas of the MAV to hardwood forest, potentially warranting restoring wild turkeys to the MAV. As part of a pilot restoration study, I gathered data on resource use and nest sites from July 2010–August 2011. I observed intraspecific niche specialization and spatial niche separation between genders. Spatiotemporal variation in resource availability and intraspecific competition appear to be factors influencing intraspecific niche specialization. Mature hardwood forest was primary habitat selected by translocated wild turkeys. Hardwood regeneration areas were primary habitat selected by hens for nesting. Visual obstruction from 0.0 m–0.5 m was important in selecting fine-scale nest sites. Hardwood regeneration areas have dual value for restoring wild turkeys to the MAV: future mature hardwood areas; and potential nest sites.

Kritische Knochendefekte stellen heutzutage ein ungelöstes Problem in der klinischen Praxis dar, da die verfügbaren prothetischen Optionen oft die mechanische Anpassung an das Gewebe nicht gewährleisten oder zu wichtigen immunologischen und Implantat-bedingten Komplikationen führen. In diesem Kontext ermöglichen Tissue Engineering-Ansätze neue Strategien, um in vitro Zell-Material Interaktionen zu untersuchen und so die Implantatmaterialien zu optimieren. In dieser Arbeit habe ich Zell-Material Interaktionen eines neuen Kollagen-basierten Scaffolds untersucht, das langfristig als Trägerstruktur für eine zellbasierte Therapie für kritische Knochendefekte entwickelt werden soll. Im Rahmen der Dissertation konnte ich belegen, dass die Kollagen-basierten makroporöse Mikrocarrier für die Zellvermehrung humaner mesenchymaler Stammzellen (MSC) und deren osteogene Differenzierung unter GMP Bedingungen verwendet werden können. Außerdem habe ich die die Kokultur von hämatopoietischen Stammzellen des Knochenmarks und multiplen Myelomzellen funktionell charakterisiert. Ich konnte erstmals Kulturbedingungen etablieren, die die Langzeitkultur ohne die Verwendung von Zytokinen ermöglicht. Mittels dieser Kokultur konnte ich ein Knochenmarknischen-Modell etablieren und die Untersuchung der Expression von zentralen Signalkaskaden der Homöostase dieser Nische untersuchen. Ich konnte die Expression von zwei verschiedenen Isoformen von Osteopontin nachweisen, die in Tiermodellen nicht gefunden werden. Diese Isoformen des Osteopontins habe ich kloniert und die rekombinanten Isoformen exprimiert und ihre Rollen in der Homöostase der Knochenmarknische untersucht. Critical size bone defects represent nowadays an unresolved problem in the clinical practice, where the available prosthetic options often lack adequate mechanical matching to the host tissue or lead to important immunological and implant-related complications. In this context, Tissue Engineering approaches promise more effective strategies to study cell-material interactions in vitro and consequently optimize implant materials. In this work, I investigated the cell-scaffold interactions of a new collagen-based scaffold for a putative cell-based therapy for critical size defects to be developed. In the context of this thesis, I could demonstrate that the collagen-based macroporous microcarriers could be employed for the expansion and osteogenic differentiation of human mesenchymal stromal cells (MSCs) under GMP-compliant conditions. Moreover, I functionally characterized the co-culture of bone marrow hematopoietic stem cells and multiple myeloma cells. I was for the first time able to establish culture conditions allowing their long-term culture in absence of externally supplemented cytokines. Using this co-culture, I was able to establish a bone marrow niche model to investigate the expression of key signaling pathways involved in the niche´s homeostasis. I was able to demonstrate the expression of two different isoforms of Osteopontin, that could not previously be detected in animal models. Finally, I cloned these Osteopontin isoforms, expressed recombinant versions of the isoforms, and investigated their roles in the homeostasis of the bone marrow niche. / Kritische Knochendefekte stellen heutzutage ein ungelöstes Problem in der klinischen Praxis dar, da die verfügbaren prothetischen Optionen oft die mechanische Anpassung an das Gewebe nicht gewährleisten oder zu wichtigen immunologischen und Implantat-bedingten Komplikationen führen. In diesem Kontext ermöglichen Tissue Engineering-Ansätze neue Strategien, um in vitro Zell-Material Interaktionen zu untersuchen und so die Implantatmaterialien zu optimieren. In dieser Arbeit habe ich Zell-Material Interaktionen eines neuen Kollagen-basierten Scaffolds untersucht, das langfristig als Trägerstruktur für eine zellbasierte Therapie für kritische Knochendefekte entwickelt werden soll. Im Rahmen der Dissertation konnte ich belegen, dass die Kollagen-basierten makroporöse Mikrocarrier für die Zellvermehrung humaner mesenchymaler Stammzellen (MSC) und deren osteogene Differenzierung unter GMP Bedingungen verwendet werden können. Außerdem habe ich die die Kokultur von hämatopoietischen Stammzellen des Knochenmarks und multiplen Myelomzellen funktionell charakterisiert. Ich konnte erstmals Kulturbedingungen etablieren, die die Langzeitkultur ohne die Verwendung von Zytokinen ermöglicht. Mittels dieser Kokultur konnte ich ein Knochenmarknischen-Modell etablieren und die Untersuchung der Expression von zentralen Signalkaskaden der Homöostase dieser Nische untersuchen. Ich konnte die Expression von zwei verschiedenen Isoformen von Osteopontin nachweisen, die in Tiermodellen nicht gefunden werden. Diese Isoformen des Osteopontins habe ich kloniert und die rekombinanten Isoformen exprimiert und ihre Rollen in der Homöostase der Knochenmarknische untersucht.

Les modèles de niche écologique (ENM) sont largement utilisés pour prédire la distribution actuelle et future des espèces. Ils peuvent être des outils complémentaires pour évaluer la qualité de niche des potentielles aires de lâchers, un facteur clé pour le succès des translocations. Pourtant, les ENM sont encore rarement appliqués dans les translocations et on a encore peu de preuves de leur intérêt pour les translocations. Nous avons centré nos recherches sur des programmes bien établis de renforcement des populations de deux espèces d'Outarde houbara, l'espèce nord-africaine (Chlamydotis undulata) et l'espèce migratrice asiatique (C. macqueenii). Grâce à un suivi intensif de populations sauvages relictuelles et d'individus élevés en captivité et lâchés sur une large partie de leur aire de répartition, ces programmes fournissent un cadre d'étude idéal pour envisager des questions complexes sur la pertinence des ENM dans la translocation. Nous avons relié la survie individuelle, une composante essentielle de la dynamique des populations, à la qualité de niche, en réalisant notamment un lâcher expérimental de 180 individus le long du gradient de qualité de la niche. Nous avons ensuite testé si les outardes lâchées utilisent la même niche écologique que les sauvages pour valider l'utilisation ultérieure des projections des modèles. Enfin, nous discutons de l'implication des changements globaux pour les translocations d'Outarde houbara. Ces approches empiriques reliant la qualité de niche aux processus démographiques constituent un pas en avant pour une meilleure compréhension de la pertinence des ENM pour les translocations d'espèces dans un environnement changeant. / Ecological niche models (ENM) are widely used to predict the current and future distribution of species. They could be used as complementary tools to assess the niche suitability of potential release areas, a key parameter for improving success in conservation translocation. Yet, ENM are still rarely applied and general evidence about the benefit of these models in translocation is still lacking. We focused our research on reinforcement programs of two species of Houbara Bustard, the sedentary North African species (Chlamydotis undulata undulata) and the migratory Asian species (C. macqueenii). Through the extensive monitoring of remnant wild populations and captive-born released individuals over a large proportion of their distribution range, these programs provide an ideal study framework to address complex questions regarding the relevancy of ENM in translocation conservation. First, we linked individual survival, a crucial component of population dynamics, to niche suitability. Then, we performed an experimental release and monitoring of 180 individuals along a gradient of habitat suitability as predicted by ENM to test the effect of release site suitability on survival of captive-born individuals. We then tested if released houbaras use the same ecological niche than wild houbaras, as a validation step to support the subsequent use of niche modelling. Finally, we discussed the implications of global change for ongoing translocation programs of Houbara Bustard. Empirical approaches linking habitat suitability with demographic processes provide a step towards a better understanding of the relevance of ENM for conservation translocations in changing environments.

Mutualism is an understudied phenomenon across taxa, but is important to the persistence and structure of communities. The role of positive interactions in supporting threatened species is critically important given that freshwater fish are extremely vulnerable due to the combined effects of climate change and habitat degradation. To disentangle complex positive multi-species interactions, I applied both Eltonian and Hutchinsonian niche approaches to the reproductive mutualism of leuciscids known as nest association, a form of alloparental care characterized by an adult of one species guarding the offspring of another. I conducted this study with the leuciscid community of Toms Creek in which Bluehead Chub Nocomis leptocephalus is the primary nest builder and nest associate species include Rosyside Dace Clinostomus funduloides, Rosefin Shiner Lythrurus ardens, Mountain Redbelly Dace Chrosomus oreas, Crescent Shiner Luxilus cerasinus, White Shiner Luxilus albeolus, Central Stoneroller Campostoma anomalum, Creek Chub Semotilus atromaculatus, and Blacknose Dace Rhinycthys atratulus. I applied Hutchinsonian niche theory with respect to hydrology and water temperature to define the reproductive phenology of each species involved in the reproductive mutualism, then used Eltonian niche theory to define the role of each species in parental and alloparental care. My dual niche theory approach elucidates the reproductive requirements of each species and quantifies the contribution of nest associates to alloparental care, which historically have not been included in definitions of nest association. My results show that Blacknose Dace Rhinichthys atratulus and Creek Chub Semotilus atromaculatus are not true nest associates of Bluehead Chub Nocomis leptocephalus based on their reproductive phenology and observed nesting behaviors. In contrast to previous work on nest association, most associate species did contribute to parental care through nest defense and vigilance behaviors, and their specific behaviors were influenced by morphology. By clearly defining the reproductive niches of these species, this study lays the groundwork for future investigation of how various climate scenarios may impact multi-species reproductive mutualisms. / Master of Science / Positive interactions are understudied throughout ecology, but are important to the persistence and structure of communities. The role of positive interactions in supporting threatened species is critically important given that freshwater fish are at high risk of extinction due to the combined effects of climate change and habitat degradation. To understand the complex positive interactions in freshwater fish, I applied both niche theory approaches to a mutualistic reproductive interaction known as nest association, where an adult of one species builds a nest on which it guards the offspring of other species (formally: a type of alloparental care). I conducted this study with the leuciscid (commonly known as minnow) community of Toms Creek in which Bluehead Chub Nocomis leptocephalus is the primary nest builder and nest associate species include Rosyside Dace Clinostomus funduloides, Rosefin Shiner Lythrurus ardens, Mountain Redbelly Dace Chrosomus oreas, Crescent Shiner Luxilus cerasinus, White Shiner Luxilus albeolus, Central Stoneroller Campostoma anomalum, Creek Chub Semotilus atromaculatus, and Blacknose Dace Rhinycthys atratulus. For each species involved in the reproductive mutualism, I defined the seasonal timing of reproduction with respect to hydrology and water temperature, then defined their role in parental and alloparental care. My approach clarifies the reproductive requirements of each species and establishes the contributions of associate species to alloparental care, which historically have not been included in definitions of nest association. My results show that Blacknose Dace Rhinichthys atratulus and Creek Chub Semotilus atromaculatus are not true nest associates of Bluehead Chub Nocomis leptocephalus based on their reproductive requirements and observed nesting behaviors. In contrast to historic descriptions of nest association, most associate species did contribute to parental care through nest defense and vigilance behaviors, and their specific behaviors were influenced by their size and physical adaptations. By clearly defining the reproductive needs and roles of these species, this study lays the groundwork for future investigation of how various climate scenarios may impact positive relationships among species.

The problem of multimodal functional optimization has been addressed by much research producing many different search techniques. Niche Genetic Algorithms is one area that has attempted to solve this problem. Many Niche Genetic Algorithms use some type of radius. When multiple optima occur within the radius, these algorithms have a difficult time locating them. Problems that have arbitrarily close optima create a greater problem. This paper presents a new Niche Genetic Algorithm framework called Dynamic-radius Species-conserving Genetic Algorithm. This new framework extends existing Genetic Algorithm research. This new framework enhances an existing Niche Genetic Algorithm in two ways. As the name implies the radius of the algorithm varies during execution. A uniform radius can cause issues if it is not set correctly during initialization. A dynamic radius compensates for these issues. The framework does not attempt to locate all of the optima in a single pass. It attempts to find some optima and then uses a tabu list to exclude those areas of the domain for future iterations. To exclude these previously located optima, the framework uses a fitness sharing approach and a seed exclusion approach. This new framework addresses many areas of difficulty in current multimodal functional optimization research. This research used the experimental research methodology. A series of classic benchmark functional optimization problems were used to compare this framework to other algorithms. These other algorithms represented classic and current Niche Genetic Algorithms. Results from this research show that this new framework does very well in locating optima in a variety of benchmark functions. In functions that have arbitrarily close optima, the framework outperforms other algorithms. Compared to other Niche Genetic Algorithms the framework does equally well in locating optima that are not arbitrarily close. Results indicate that varying the radius during execution and the use of a tabu list assists in solving functional optimization problems for continuous functions that have arbitrarily close optima.