Secondary ion mass spectrometry (SIMS) is a surface analysis technique which characterizes species sputtered by an energetic particle beam. Bombardment with cluster projectiles offers the following notable advantages over bombardment with atomic ions or small clusters: enhanced emission of molecular ions, low damage cross-section, and reduced molecular fragmentation. Additionally, in the case of Au4004 and C60 impacts, desorption originates from nanometric volumes. These features make clusters useful probes to obtain molecular information from both nano-objects and nano-domains. The "event-by-event bombardment/detection mode" probes nano-objects one-at-a-time, while collecting and storing the corresponding secondary ion (SI) information. Presented here are the first experiments where free-standing nano-objects were bombarded with keV projectiles of atomic to nanoparticle size. The objects are aluminum nano-whiskers, 2 nm in diameter and ~250 nm in length. Au4004 has a diameter of ~2 nm, comparable to the nominal diameter of the nanowhiskers. There are notable differences in the SI response from sample volumes too small for full projectile energy deposition. The whisker spectra are dominated by small clusters?the most abundant species being AlO- and AlO2-. Bulk samples have larger yields for AlO2- than for AlO-, while this trend is reversed in whisker samples. Bulk samples give similar abundances of large SI clusters, while whisker samples give an order of magnitude lower yield of these SIs. Effective yields were calculated in order to determine quantitative differences between the nano-objects and bulk samples. The characterization of individual nano-objects from a mixture is demonstrated with negatively charged polymer spheres that are attracted to and retained by the nano-whiskers. The spheres are monodisperse polystyrene nanoparticles (30nm diameter). Our results show that the event-by-event mode can provide information on the nature, size, relative location, and abundance of nano-objects in the field of view. This study presents the first evidence of quantitative molecular information originating from nano-object mixtures. Biologically relevant systems (solid-supported lipid bilayers) were also characterized using Au5 , Au4004 and C60 . Organization-dependent SI emission was observed for phosphocholine bilayers. Lipid domain formation was also investigated in bilayers formed from cholesterol and a mixed lipid system. Trends in the correlation coefficient suggest that cholesterol segregates from the surrounding lipid environment during raft formation.

Master´s thesis proposes a marketing communication for 1. FC Brno joint-stock company. Studied company´s business activities are focused on specific segment of market with substantial use of marketig tools. Prezentation itself is based on some important analysis.

Ephemerality is one of the most essential characteristics of fashion, since fashion by definition is existing in the present, in the fleeing moment. Also, fashion is closely related to art and there is currently no clear theoretical distinction made regarding the boundary between these two domains. In this essay I have explored the concept of transiency and the relationship between fashion and art in relation to fashion in general and to the collection Haute Papier, made by the fashion designer Bea Szenfeld, in specific. The Haute Papier collection is constructed almost entirely out of simple white paper sheets and of this reason it can be regarded as ephemeral. The material also positions the garments in a void between fashion and art, simultaneously existing in both domains and in none of them. I have conducted an object analysis of three of the garments from Haute Papier with the aim of discerning aspects of ephemerality, aspects that criticizes the age in which we live and how the collection positions itself in regard to fashion and art. The theories that I have applied to my material emanates from different aspects of transiency and the fashion and art relationship and includes, among others, theories from Walter Benjamin and Barbara Vinken and extractions from the anthology Fashion and Art by Adam Geczy and Vicki Karaminas. In the analysis it became visible that transiency can be discerned in many varying aspects of the collection, for example in the material and in the time in which fashion operates. What also became visible was that the definition of the object, as art or as fashion, above all depends on which system it is part of, but that it also can change this definition if the context changes.

Master´s thesis is considered with design of algorithms for new module of company information system. In the beginning of thesis there are characterized types of ways to describe an information systems. For specification of described system is briefly defined IBM Lotus Notes environment. Next chapter is about object-oriented analysis and design of a module of information system by using UML´s diagrams in modeling tool Enterprise Architect. In the third chapter is made analysis and design of module´s connection with current system, specificly update data in form. Thesis shows designed algorithms in environment of Lotus Domino Designer by using LotusScript and SQL languages and Lotus Domino Connector for access into the database by using ODBC. In last part of thesis is proposed to use a mapping tool to mapp the ITC infrastructure by using Change management process according to ITIL method, to manage all the changes in developing system effectively.