Eye disorders are on the rise as a result of an ageing population, an increasing obesity problem and a growth in the number of diabetic patients. Conventional ophthalmic formulations do not maintain therapeutic drug concentration in the target tissues for a long duration due to the physiological and anatomical eye barriers. Novel delivery systems such as nanoparticles have been explored to enhance the delivery of therapeutic agents to the eye. These delivery systems have in general been assessed using in-vivo animal models, despite ethical concerns for animal wellbeing. The aims of this thesis were to synthesise and characterise four amphiphilic polymers, subsequently prepare and characterise four nano sized polymeric self-assemblies loaded with triamcinolone acetonide (TA), develop an in-vitro porcine eye model and to evaluate the permeation of nano sized self-assemblies using the developed model. Four comb-shaped amphiphilic polymers (Pa5, Pa5-MPEG, Ch5 and Da10) were synthesised with a high yield (>81%) and good reproducibility. These polymers formed spontaneous positive self-assemblies in aqueous media (114-314 nm). The mean hydrodynamic diameters of the positive spontaneous self-assemblies entrapping TA were in the range of 200–334 nm loading high concentrations (455-1263 μg mL-1) of TA, much greater than the TA inherent aqueous solubility or concentrations achieved using conventional solubilisers. A porcine in-vitro eye model was developed to assess drug permeation through anterior and posterior ocular tissues. The model was partially validated using tritiated water and a series of hydrophilic markers with increasing molecular weights. The integrity of porcine ocular tissue was checked by monitoring the permeation of tritiated water to ensure the membrane intactness. Tritiated water permeation at 15 min was exploited as a potential method to normalise drug flux, as tritiated water percentage permeation at 15 min had an inverse relationship with tissue thickness (R2 = 0.66), to reduce the inherent variability between tissue samples thus increasing the accuracy of the in-vitro eye model. Four markers (fluorescein sodium salt, 4, 10 and 20 kDa FITC-dextran) were used for the purpose of investigating the effect of increasing molecular weight on ocular tissue permeability. The permeability of the markers displayed an inverse relationship and abrupt decline with Mw in terms of the permeability through scleral and corneal tissues of human and porcine and the molecular weight of the markers. The developed porcine in-vitro eye model showed good correlation with the human in-vitro model providing strong evidence it can be used to screen potential formulations before testing in-vivo. The TA loaded self-assemblies and a few chemical enhancers (glutamic acid, tween 80, chitosan, Pa5 and elevate temperature (45 °C)), selected to assist drug delivery via two routes (paracellular and transcellular), were tested using the developed in-vitro eye model. The results showed there was no marker permeation enhancement effect in porcine and human ocular tissues using chemical enhancers. In summary, a porcine in-vitro eye model was developed to assess hydrophobic and hydrophilic penetrant permeation across anterior and posterior ocular tissues. The porcine in-vitro eye model showed good correlation with the human in-vitro model providing strong evidence that the porcine in-vitro eye model can be used to screen potential formulations before testing in-vivo using the porcine model which ultimately might correlate well with the in-vivo human responses. Although TA self-assemblies did not significantly increase drug flux through human or porcine scleral tissues, it might be of interest for ophthalmic topically administered formulations due to their positive charge and small nano size.

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Made available in DSpace on 2016-08-10T10:56:16Z (GMT). No. of bitstreams: 1 REJANE CARVALHO AIRES.pdf: 2805457 bytes, checksum: 658de5ee5722a31268de1b973b1273c3 (MD5) Previous issue date: 2010-05-06 / This is a study on the environmental impact on tear film in employees of a sugar and alcohol mill in Rubiataba GO. The aim of this study was to evaluate the tear film in employees of of a sugar and alcohol mill in Rubiataba GO. Tear film of 155 employees, working either inside the mill or in the field, female and male, with neither previous history of ocular nor usage of systemic or topical medication, was assessed. Following detailed anamnesis of each employee, tear ferning test, Schirmer s test, tear film break-up time (TFBUT), and rose Bengal staining were performed in the ocular region in two periods of the year (rain and dry seasons). Meteorological data were given by the Meteorological and Hydrological System of the State of Goias. Analysis of data were performed by using the Statistical Package for the Social Science (SPSS) software, version 15.0, by the Fisher s exact test, Wilcoxon signed-rank test, McNemar test and Mann-Whitney U test. Results were analyzed in three steps. First, one group of patients with normal ophthalmic tests and another group of patients with altered results were compared, and the effects of sex, type of work and period of the year on the results were evaluated. There was no significant difference. A significant improvement in TFBUT was observed (P = 0.035) from May to September in 2009. In the second analysis, all ophthalmic tests results were gathered together. A significantly higher prevalence (P < 0.041) of symptoms was observed in May compared to September in 2009. Ocular symptoms were referred exclusively by employees in the mill. Both TFBUT (P < 0.001) and rose Bengal test (P < 0.014) improved significantly, while Schimmer s test became worse (P < 0.003) from May to September in 2009. However, these results had no clinical relevance. In the third analysis, a comparison between symptomatic and asymptomatic was performed; worse TFBUT (P < 0.001) and rose Bengal test (P < 0.001) results were found in symptomatic comparatively with asymptomatic subjects in both periods of the year. These results suggest that employees in the mill were probably more aware of their symptoms than those in the field, and that sugarcane burning throughout the year may cause tear film instability. Furthermore, employees in the mill may have used adequate personal protective equipment and, therefore, there was no difference between the two periods of year. / Este é um estudo do impacto ambiental sobre o filme lacrimal de trabalhadores de usina de açúcar e álcool em Rubiataba - GO. O objetivo deste trabalho foi avaliar o filme lacrimal em trabalhadores de uma usina de açúcar e álcool em Rubiataba - GO. Foi avaliado o filme lacrimal de 155 trabalhadores no ambiente da usina e do campo, dos sexos femininos e masculinos, sem doenças oculares prévias e uso de medicações sistêmicas ou tópicas. Após anamnese detalhada de cada trabalhador, foram aplicados na região ocular os testes de cristalização da lágrima e de Schirmer, tempo de rotura do filme lacrimal (TRFL) e coloração com rosa bengala, em dois períodos do ano (chuvoso e seco). Os dados meteorológicos foram fornecidos pelo Sistema de Meteorologia e Hidrologia do Estado de Goiás. A análise dos dados foi feita com o software Statistical Package for the Social Science (SPSS) versão 15.0, aplicando-se os testes exato de Fisher, Wilcoxon, McNemar e U Mann Whitney. Os resultados foram avaliados em três etapas. Na primeira análise foi formado um grupo com resultados de testes oftalmológicos normais e outro com alterados, e depois comparados para verificar a influência do sexo, do tipo de trabalho e do período do ano. Não houve diferença significativa. Nesta análise verificou-se melhora significativa no TRFL (P=0, 035) de maio para setembro de 2009. Para a segunda análise, os resultados dos testes oftalmológicos foram agrupados. Notou-se que os sintomas oculares foram significativamente mais prevalentes no mês de maio de 2009 do que no mês de setembro (P<0, 041). Os trabalhadores da usina foram os únicos a referirem sintomas oculares. Os testes oftalmológicos do TRFL (P<0, 001) e de rosa bengala (P<0, 014) apresentaram melhora estatisticamente significativa, enquanto o teste de Schirmer (P<0, 003) apresentou piora de maio para setembro de 2009. Entretanto, estes resultados não tiveram nenhuma relevância clínica. Na terceira análise, foram comparados os grupos de sintomáticos e assintomáticos. Os resultados de TRFL (P<0, 001) e do teste de rosa bengala (P<0, 001) foram piores nos grupos de sintomáticos do que no grupo de assintomáticos, nos dois períodos do ano. Os resultados sugerem que os trabalhadores do ambiente da usina perceberam mais os sintomas oculares do que os trabalhadores do campo, possivelmente pela queima da cana-de-açúcar, presente durante todo o ano, poderia ter desencadeado uma instabilidade do filme lacrimal por não ter havido uso adequado dos equipamentos de proteção individual. Não havendo, portanto, diferenças entre os dois períodos do ano.

Orientadores: Jose Paulo Cabral de Vasconcellos, Vital Paulino Costa / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas / Made available in DSpace on 2018-08-12T19:09:11Z (GMT). No. of bitstreams: 1 Medina_FlavioMacCord_M.pdf: 2280525 bytes, checksum: d64f96bf19c317e1e22a704a8562d282 (MD5) Previous issue date: 2009 / Resumo: Objetivo: Avaliar a reprodutibilidade do teste de sobrecarga hídrica (TSH) em diferentes horários em que é realizado, em pacientes com glaucoma primário de ângulo aberto (GPAA) e em indivíduos normais. Métodos: Quinze pacientes com GPAA e 30 indivíduos normais foram submetidos a três TSHs, realizados em diferentes horários do dia (às 7:00, 12:00 e 17:00), em três dias diferentes. Foram comparados os resultados dos testes em pacientes com GPAA e indivíduos normais. Foram analisadas a concordância e a correlação entre os valores de medida basal, pico e variação de pressão intra-ocular (PIO) (pico de PIO - PIO basal) nos testes realizados nos diferentes horários. Apenas as medidas do olho direito foram analisadas. Resultados: Os valores médios de medida basal, pico e variação de PIO foram significativamente maiores nos pacientes glaucomatosos que nos indivíduos normais, em todos os horários (p<0,05). A análise de Bland-Altman apresentou limites de concordância de pico e variação de PIO maiores do que o clinicamente aceitável (> 3 mmHg), apesar de o teste de Pearson demonstrar boa correlação entre os resultados. Conclusões: O TSH apresenta valores de pico e variação de Pio significativamente maiores em pacientes glaucomatosos que em indivíduos normais. Os baixos níveis de concordância entre os TSHs realizados em diferentes horários do dia sugerem uma baixa reprodutibilidade do TSH, que pode limitar sua aplicabilidade para diagnóstico e acompanhamento do glaucoma / Abstract: Purpose: To evaluate the reproducibility of the water drinking test (WDT) performed at different times of the day, in primary open angle glaucoma (POAG) patients and normal individuals. Methods: Fifteen patients with POAG and 30 normal individuals underwent three WDTs at different times of the day (7 AM, 12 PM, and 5 PM) on 3 different days. Test results in POAG patients and normal individuals were compared. Agreement and correlation of intraocular pressure (IOP) baseline levels, peak levels, and IOP change (peak IOP - baseline IOP) on tests performed at different times were evaluated. Only right eye measurements were analyzed. Results: Mean baseline IOP, peak IOP and IOP change were significantly higher in POAG patients than in normal individuals, at all time intervals (p<0.05). The Bland-Altman analysis demonstrated limits of agreement for IOP peak levels and IOP changes larger than the clinically acceptable (> 3 mmHg), even though Pearson's test revealed good correlation among the results. Conclusions: The mean IOP peak and mean IOP change observed during the WDT are significantly higher in POAG patients than in control individuals. Low levels of agreement among WDTs performed at different times of the day suggest a poor reproducibility of WDT, which may limit its applicability for the diagnosis and follow-up of glaucoma / Mestrado / Oftalmologia / Mestre em Ciências Médicas

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Objetivo: Avaliar a amplitude de pulso ocular (APO) em pacientes com hipertensão arterial sistêmica, comparando com o grupo controle, e eventuais influências da espessura central da córnea e do comprimento axial (COMPRaxial) do olho na APO. Método: Foram examinados 152 olhos de 76 pacientes com hipertensão arterial sistêmica e 136 olhos de 68 indivíduos que compuseram o grupo controle. Todas as medidas foram tomadas pelo mesmo examinador, no período das 7 às 10h da manhã, na seguinte ordem: pressão arterial sistêmica, tonometria de contorno dinâmico, COMPRaxial e paquimetria (PAQ). A análise estatística foi realizada utilizando-se o teste t para médias de dados pareados e, para as correlações, os coeficientes de Pearson ou o de Spearman. Considerou-se o nível de significância de 5%. Resultados: A APO média dos olhos direitos dos pacientes com hipertensão arterial sistêmica foi 2,10 (+/- 0.9 mmHg) e a dos olhos esquerdos foi 2,03 (+/-0,828 mmHg). A PAQ média dos olhos direitos foi 532.2 mµ (+/- 39 mµ), e a dos olhos esquerdos 532.1 mµ (+/- 36.5 mµ), não influenciando a APO. A variável COMPRaxial para o olho direito (OD) apresentou média de 23,44 mm (+/- 1.477 mm) e para o olho esquerdo (OE) foi de 23.343 em média (+/- 1,32 mm). Houve significância estatística quando estudada a influência do COMPRaxial sobre a APO, demonstrando correlação inversamente proporcional. A média da APO dos controles foi de 2,10 (+/- 0.9 mmHg) e para o OD e OE média de 2,03 (+/-0,828 mmHg). Quando comparados os valores médios da APO dos casos e controles, a diferença foi estatisticamente significante, sendo os valores dos controles maiores do que os encontrados nos pacientes com hipertensão arterial sistêmica (p <0 ,001). Constatou-se que existe diferença entre caso e controle, tanto para o OD quanto para o OE. Conclusões: O valor médio de APO foi menor nos pacientes com hipertensão arterial sistêmica do que nos controles; a APO não foi influenciada pela espessura central da córnea; e olhos com maior COMPRaxial apresentaram APO menor / Objective: The objectives of this study were to assess the ocular pulse amplitude (OPA) in patients with hypertension, compared with control group and to evaluate possible influences of central corneal thickness and axial length of eye in OPA. Method: We evaluated 152 eyes of 76 patients with hypertension and 136 eyes of 68 individuals who comprised the control group. All measurements were made by the same examiner in the period of 7 am and 10 am, in following order: blood pressure, dynamic contour tonometry, axial length and pachymetry. Statistical analysis was performed using the t test for paired data and averages for correlations, the Pearson correlation coefficients of Spearman. Considered the significance level of 5%. Results: The average OPA in right eyes of patients with hypertension was 2.10 (standard deviation (SD) 0.9 mmHg) and in left eyes was 2.03 (SD =0.828 mmHg). The average pachymetry of right eyes was 532.2 mµ (+/- 39 mµ); and for left eyes was 53.1 mµ ( +/-36.5 mµ). The variable diameter axial in right eye showed an average of 23.44 mm (+/- 1.477 mm); for the left eye 23,343 mm (+/- 1.32). There was statistical significance when studied the influence of the axial diameter of OPA, with inverse correlation. The average of controls OPA was presented mean 2.10 (+/- 0.9 mmHg) for the right eye and the left mean 2.03 (+/- 0.828 mmHg). When comparing the mean values of OPA in cases and controls the difference was statistically significant, the values of the controls are larger those found in patients with hypertension (p<0,001), so it was found difference between cases and controls, both for the right and left eye. Conclusions: The mean OPA was lower in patients with hypertension than in controls, the OPA was not influenced by central corneal thickness, and eyes with greater axial length showed lower OPA

A relação trabalho e visão é tão remota que determinou a adaptação da espécie humana ao meio em que está inserida. Demonstrou-se que trabalhar em um ambiente confortável, sem doenças ocular, favorece o desempenho visual e aumenta a produtividade. Conhecer os efeitos do monitor de computador (VDT) sobre o sistema visual significa planejar melhor o ambiente de trabalho e a atividade laboral para se obter máximo conforto e produtividade. Este estudo teve como objetivo geral analisar as alterações do piscar, do filme lacrimal e da superfície ocular induzidas pelo uso de monitor de computador (VDT). Para sua realização efetivou-se um estudo transversal, descritivo, observacional, analítico, realizado na Volkswagen do Brasil, São José dos Pinhais (PR), tendo como amostra, a população de funcionários do setor administrativo que utiliza VDT, com idade acima de 18 anos, sem doenças da superfície ocular, sistema palpebral e lacrimal. O ambiente de trabalho foi caracterizado pela temperatura, grau de umidade, velocidade do ar e iluminamento. Preencheram os critérios de inclusão 108 funcionários (idade média 34,1±7,88 anos e tempo mínimo de utilização do VDT 6 horas/dia). Foram avaliados as sintomatologias, filmagem da face para mensuração do tempo entre piscadas na situação de conversação, exame do olho externo e biomicroscopia das pálpebras, conjuntiva e córnea, filmagem videoceratoscópica para determinar tempo de ruptura do filme lacrimal, avaliação da quantidade de lágrima do lago lacrimal pelo Zone-Quick Phenol Red Thread tear test, avaliação do epitélio corneano pelo teste da lissamina verde, filmagem da face dos sujeitos para mensuração do tempo entre piscadas em leitura de VDT. Ao final da jornada de trabalho, foram feitas a reavaliação do tempo de ruptura do filme lacrimal da quantidade de lágrima e a avaliação do epitélio corneano. Compararamse o tempo entre as piscadas em conversação e em uso de VDT e o tempo de ruptura do filme lacrimal, antes e ao final da jornada de trabalho. A comparação desses tempos classificou os sujeitos como expostos, com tempo médio de ruptura do filme lacrimal menor que o tempo médio entre piscadas e não expostos com tempo médio de ruptura do filme lacrimal maior que o tempo médio entre piscadas. Nas condições do estudo, em jornada de trabalho com tempo mínimo de 6 horas de uso de VDT, houve aumento da exposição da superfície ocular causada pelo aumento do tempo entre piscadas. O aumento da exposição da superfície ocular foi causado pela diminuição do tempo de ruptura do filme lacrimal constatado ao final da jornada de trabalho. O tempo entre piscadas na situação de leitura em VDT que foi significantemente maior que na situação de conversação. O tempo de ruptura do filme lacrimal, ao final da jornada de trabalho, foi significativamente menor que em seu início. A exposição ocular observada durante a jornada de trabalho com usuários de VDT, nas condições deste estudo, não causou alteração epitelial detectável ao teste da lissamina verde. Não houve alteração do volume lacrimal em olhos com exposição, antes da jornada de trabalho com VDT, nem houve alteração do volume lacrimal em olhos com exposição ocular, após a jornada de trabalho com VDT. / The relationship between work and vision is so remote that has determined the adaptation of human beings to the environment in which they are inserted. It has been demonstrated that working in a pleasant environment, without any ocular disease, has favored visual performance and increased productivity. Knowing the effects of video terminal display (VDT) on the visual system helps plan labor environments and labor activities more effectively so that optimal comfort and productivity are obtained. This transversal study, performed at Volkswagen do Brasil, São José dos Pinhais, PR, has the objective to investigate the influence of VDT labor activities on blinking, tear film and ocular surface, ocular exposure and amount of tear in the lacrimal lake. The population sample comprised VDT administrative department employees older than 18, without diseases on the ocular surface, tear film and blinking system. The evaluation of the working environment included measurements of temperature, humidity, air speed and lighting. One hundred and eight employees met the study criteria (mean age 34.1±7.88 and minimal VDT use time of 6 hours/day). Several evaluations were performed: the ocular surface symptomatology; the measurement of the blink time in conversation situations; the examination of the ocular surface and lacrimal film; a videokeratoscopy to establish the tear film break-up time; evaluation of the amount of tears of the lacrimal lake by means of the Zone-Quick Phenol Red Thread tear test; evaluation of the corneal epithelium by means of the lissamine green test; and determination of the blink time in VDT reading situations. At the end of the working period, the amount of tears and the corneal epithelium structure were re-evaluated. Comparisons between the blink time in conversation and VDT reading situations were performed before and at the end of the working day. The comparison data obtained classifies the subjects into two categories: exposed, with a tear film break-up time shorter than the mean time between blinks; and non-exposed, with tear film break-up time longer than the mean time between blinks. The findings obtained reveal that there was an increase in the ocular surface exposure caused by the increase in the time between blinks during the working period and increase of the ocular surface exposure caused by the decrease in the tear film break-up time determined at the end of the working day. The time between blinks at VDT reading situations was significantly longer than at conversation situations. The tear film break-up time at the end of the working day was significantly shorter than at the beginning. The ocular exposure during the VDT use has not caused epithelium alterations detectable at the lissamine green test. There were no alterations in the amount of tears in the eyes which underwent ocular exposure after VDT use situations.

by Maksudul Islam. / Publication date from spine. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1995. / Includes bibliographical references (leaves 58-65). / Questionaire also in Chinese. / Chapter CHAPTER1 --- ABSTRACT --- p.1 / Chapter CHAPTER2 --- INTRODUCTION --- p.3 / Chapter CHAPTER3 --- OBJECTIVES --- p.7 / Chapter CHAPTER4 --- LITERATURE REVIEW --- p.8 / Chapter 4.1.1 --- BACKGROUND --- p.8 / Chapter 4.1.2 --- GENETIC AND ENVIRONMENTAL STUDY --- p.9 / Chapter 4.1.3 --- EPIDEMIOLOGY OF MYOPIA --- p.14 / Chapter 4.1.4 --- RELATIONSHIP BETWEEN MYOPIA & OPTICAL COMPONENTS --- p.18 / Chapter 4.1.5 --- AXIAL LENGTH --- p.19 / Chapter 4.1.6 --- VITREOUS LENGTH --- p.20 / Chapter 4.1.7 --- CHANGES OF REFRACTION IN SCHOOL CHILDREN --- p.21 / Chapter 4.1.8 --- DEVELOPMENT OF MYOPIA --- p.23 / Chapter 4.1.9 --- EMMETROPIZATION --- p.26 / Chapter 4.1.10 --- SEX --- p.27 / Chapter 4.1.11 --- FACTORS --- p.29 / Chapter CHAPTER5 --- METHODS --- p.34 / SUBJECTS --- p.34 / PROCEDURE --- p.35 / Chapter CHAPTER6 --- RESULTS --- p.37 / Chapter 6.1.1 --- THE DISTRIBUTION OF REFRACTION --- p.37 / Chapter 6.1.2 --- THE RELATION BETWEEN AGE WITH REFRACTION AND OPTICAL COMPONENTS --- p.38 / Chapter 6.1.3 --- RELATION BETWEEN SEX AND REFRACTION --- p.39 / Chapter 6.1.4 --- RELATION BETWEEN REFRACTION AND OPTICAL COMPONENTS --- p.39 / Chapter 6.1.5 --- RELATION BETWEEN REFRACTION AND VISUAL TASK --- p.40 / Chapter 6.1.6 --- RELATION BETWEEN OCULAR COMPONENTS AND VISUAL TASK --- p.42 / Chapter 6.1.7 --- RELATION BETWEEN OCULAR COMPONENTS AND REFRACTION TO PARENTAL MYOPIA AND EDUCATION --- p.44 / Chapter CHAPTER7 --- DISCUSSION --- p.47 / Chapter CHAPTER8 --- REFERENCES --- p.58 / TABLES --- p.66 / FIGURES --- p.86 / APPENDIX I-II QUESTIONNAIRE IN CHINESE --- p.100 / APPENDIX III-IV QUESTIONNAIRE IN ENGLISH TRANSLATION --- p.110

Made available in DSpace on 2014-06-11T19:22:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-02-26Bitstream added on 2014-06-13T20:28:22Z : No. of bitstreams: 1 addad_mt_me_botfm.pdf: 674800 bytes, checksum: 101691ff84e6e0c57b104e36c6ab28a2 (MD5) / Comparar pico e flutuação de pressão intra-ocular (PIO) entre curva tensional diária e teste de sobrecarga hídrica, detectar os horários de pico de PIO, comparar os valores da PIO nos horários das 6 horas da manhã deitado e sentado. 30 olhos direitos de 30 pacientes portadores de glaucoma primário de ângulo aberto (GPAA) ou hipertensão ocular (HO), foram submetidos à curva tensional diária de 24 horas (CTD) e teste de sobrecarga hídrica (TSH). Foram avaliados: pico (maior valor da PIO durante a CTD e TSH ) e flutuação (maior valor da PIO menos o menor valor da PIO durante o CTD e TSH). A PIO foi avaliada, com tonômetro de aplanação de Goldmann às 9, 12, 15, 18, 21, 24 e 6h, com paciente sentado. As medidas das 3 e 6 horas da manhã foram realizadas no leito com tonômetro de Perkins com o paciente em posição supina (deitado). Imediatamente após a medida no leito deitado, foi realizada outra medida das 6 horas com o paciente sentado. Para realização do TSH, os pacientes ingeriam 1 litro de água, em até 5 minutos. A PIO foi avaliada imediatamente antes e durante uma hora após a ingestão de água, com intervalos de 15 minutos entre as medidas. O TSH foi realizado logo após a avaliação da PIO às 6h da manhã. Foram calculados valores de média (M) e desvio padrão (s) para pico e flutuação e mediana (Md) e quartís (Q2) para flutuação em porcentagem (%). Para comparação de médias foi realizado o teste t de Student para amostras dependentes. Foi utilizado teste de Wilcoxon para comparação de medianas entre amostras dependentes. Para todas as análises foi considerado nível de significância p <0,05. A média dos valores de pico de PIO foi significativamente maior na CTD (20,39 ±1,03 mmHg) do que no TSH (17,37 ± 0,78 mmHg), p < 0,001. A média dos valores de flutuação também foi maior na CTD (10,17 ± 0,90) do que no TSH (5,0 ± 0,45), p < 0,001... / Comparing peak and fluctuation of intraocular pressure (IOP) between diurnal tension curve and water drinking test, detect the peak of IOP, compare the IOP at 6AM in supine and sitting position. 30 right eyes of 30 glaucoma patients with open angle glaucoma (OAG) or ocular hypertension (HO) were submitted to water drinking test (WDT) and diurnal tension curve (DTC). Were evaluated: peak (higher value of IOP observed during WDT and DTC), fluctuation (difference between highest and lowest value of IOP WDT and DTC). IOP was measured with applanation Goldmann tonometry at 9AM, 12AM, 3PM, 6PM, 9PM, 12PM and 6 AM, with the patient sitting. Measurements of 3AM and 6AM were made in bed with Perkins tonometer with the patient in the supine position. Immediately after the measure in supine position, another measure was done at 6AM, with the patient seated. The WDT involved ingestion of 1 liter of tap water in 5 minutes. IOP was measured immediately before and one hour after ingestion of water at intervals of 15 minutes between measurements. The WDT was performed immediately after the measure IOP at 6 AM. For comparison of means was performed Student's t test for dependent samples. Wilcoxon test was used to compare medians between dependent samples. The level of significance was considered p <0.05. The mean peak IOP was significantly higher in the DTC (20.39 ± 1.03 mmHg) than in TSH (17.37 ± 0.78 mmHg), p <0.001. The mean fluctuation was also higher in the DTC (10.17 ± 0.90) than in WDT (5.0 ± 0.45), p <0.001. The value of the fluctuation in the DTC percentile was 90.46% (70.00, 129.90) while the WDT was 35.42% (27.92, 56.95), p <0.001. The IOP of 6 AM showed a statistically significant when performed with the patient in supine position (17,47 mmHg) and sitting (14,30 mmHg) and was significantly higher with the patient in supine position. (p < 0,001). The WDT peak and underestimates of ... (Complete abstract click electronic access below)