Conservation biologists have used reintroduction as a method to reestablish extirpated species in their native habitat. Three important aspects of a successful reintroduction effort include: (1) a habitat suitability study of the reintroduction area, including effects of migration corridors; (2) identification of possible predators of the reintroduced species; and (3) a post-reintroduction assessment including an evaluation of the species' population dynamics. In this study I examine the suitability of Guadalupe Mountains National Park (GUMO) as a reintroduction area for desert bighorn sheep. The study used landscape metrics to compare GUMO to a nearby mountain range that is currently supporting an estimated population of 400 bighorn sheep. This study identified migration corridors for bighorns throughout the region and evaluated mountain lion (a potential predator of bighorn sheep) numbers either residing in or passing through the park between the years 1997 to 2004. Results on the studies in GUMO revealed 15,884 ha of suitable habitat for bighorn sheep and provided evidence of migration routes between GUMO and neighboring mountain ranges. In terms of potential predators, a minimum of 32 resident and/or transient mountain lions occurred in GUMO over a seven year period, and a minimum of 15 cats used the park in 2002. Based on estimates of individual home range of males and females, GUMO should be able to support four to five individuals. The genetic data indicates a high number of transients or perhaps an unstable population of mountain lions that may be the result of intense hunting pressure of cats in Texas. Finally, my study simulates parameters of the population dynamics of a different species, the Arabian oryx that was reintroduced as three separate populations to the Israeli Negev between 1998 and 2005. I simulated population growth and the effect of migration corridors on species persistence. Results suggest that migration corridors are essential for a self-sustaining viable metapopulation under current natality rates. In the event that natality rates increase (as was evident in a reintroduced population of Arabian oryx in Oman), metapopulation can reach viable size with only two of the release sites (open, flat terrain) connected by migration corridors.

Habitat loss and fragmentation is a crisis affecting wildlife worldwide. In Tanzania, East Africa, a dramatic and recent (<80 years) expansion in human settlement and agriculture threatens to reduce gene flow among protected areas for many species of large mammals. Wildlife linkages can mitigate population isolation, but linkage designs lacking empirical justification may be controversial and ineffective. Connectivity conservation requires an understanding of how biogeographic factors shaped gene flow prior to habitat loss or fragmentation, however the history of interaction among populations is rarely known. The goal of my study was to provide context for connectivity conservation in central and southern Tanzania by identifying landscape features that have shaped gene flow for three ungulate species with different dispersal capabilities. I investigated historical patterns of connectivity for Maasai giraffe (Giraffa camelopardalis tippelskirchi), impala (Aepyceros melampus), and eland (Tragelaphus oryx) by estimating genetic structure among four to eight protected areas per species. Genetic structure changes very slowly among large populations and thus is likely to reflect historical processes instead of recent anthropogenic influences. I collected noninvasive DNA samples and generated microsatellite genotypes at 8 to 15 loci per species, then estimated genetic diversity metrics (allelic richness, AR, and expected heterozygosity, H[subscript E]) for each population (defined by reserve). I also calculated genetic distance (F[subscript ST] and Nei's unbiased genetic distance, D[subscript hat]) and an estimate of gene flow (Nm) between all population pairs for each species. To elucidate the possible causes of genetic structure between these populations, I tested for isolation by distance and isolation by resistance based on a suite of biogeographic factors hypothesized to affect gene flow. To do this, I created GIS-based resistance surfaces that assigned different costs of movement to landscape features. I created one or more resistance surfaces for each hypothesis of landscape effect. I used circuit theory to estimate the cumulative resistance between each pair of reserves for each weighting scheme, and then performed Mantel tests to calculate the correlation between these resistances and the observed population pairwise genetic distances (D[subscript hat]). I chose the optimal resistance model for each species as the model that was most highly correlated with observed genetic patterns. To verify that the correlation of resistance models with genetic distance was not an artefact of geographic distance, I performed partial Mantel tests to calculate correlation while controlling for the effect of geographic distance. Finally, I compared historical gene flow patterns to the distribution of contemporary human activity to predict areas that are at risk of a loss of connectivity. Indices of genetic diversity were moderate for all three species and comparable to previously reported values for other savannah ungulates. Diversity (both H[subscript E] and A[subscript R]) was highest in eland and lowest in giraffe for these populations, and was not consistently correlated with reserve size as has been reported for other species in East Africa. Although patterns in genetic distance were broadly similar across all three species there were also striking differences in connectivity, highlighting the importance of cross-species comparisons in connectivity conservation. At this scale, resistance models based on slope strongly predicted population structure for all three species; distance to water was also highly correlated with genetic distance in eland. For all three species, the greatest genetic distances occurred between populations separated by the Eastern Arc Mountains, suggesting that the topography of this area has long acted as a barrier to gene flow, but this effect is present in varying degrees for each species. I observed high levels of historical gene flow between centrally located populations (Ruaha National Park and Rungwa Game Reserve) and those in the southwest (Katavi National Park and Rukwa Game Reserve). Although human settlement in this area has been low relative to other areas, the connection between the Katavi/Rukwa and Ruaha ecosystems may be threatened by increased human activity and warrants conservation. High levels of historical gene flow were also seen between reserves in the northeast (Tarangire National Park, Swagaswaga Game Reserve) and the central and southwest populations. These connections appear highly threatened due to current land use practices, and may have already suffered a loss of gene flow. Field surveys in the lands surrounding the northeastern reserves are needed to quantify current levels of connectivity and determine whether corridors could be established to maintain or restore gene flow with other reserves. / Graduation date: 2012

This thesis considers the understudied issue of genetic engineering as it has been deployed in the literature of the late 20th century. With reference to the concept of the enlightened gender hybridity of Cyborg theory and an eye to ecocritical implications, I read four texts: Joan Slonczewski's 1986 science fiction novel A Door Into Ocean, Octavia Butler's science fiction trilogy Lilith's Brood – originally released between 1987 and 1989 as Xenogenesis – Simon Mawer's 1997 literary novel Mendel's Dwarf, and the first two books in Margaret Atwood's speculative fiction MaddAddam series: 2003's Oryx and Crake and 2009's The Year Of the Flood. I argue that the inclusion of genetic engineering has changed as the technology moves from science fiction to science fact, moving from the fantastic to the mundane. Throughout its recent literary history, genetic engineering has played a role in complicating questions of sexuality, paternity, and the division between nature and culture. It has also come to represent a nexus of potential cultural change, one which stands to fulfill the dramatic hybridity Haraway rhapsodized in her "Cyborg Manifesto" while also containing the potential to disrupt the ecocritical conversation by destroying what we used to understand as nature. Despite their four different takes on the issue, each of the texts I read offers a complex vision of utopian hopes and apocalyptic fears. They agree that, for better or for worse, genetic engineering is forever changing both our world and ourselves.

This thesis explores the role and representation of the natural world in a selection of Canadian dystopian novels: After the Fact (1986) by Hélène Holden, Voices in Time (1986) by Hugh MacLennan, Oryx and Crake (2004) and The Handmaid's Taie (1985) by Margaret Atwood. In order to argue that Canadian dystopian fiction varies from conventional literary dystopias because of its predominant use of nature, this thesis first examines the influence that archetypal images and symbols of nature have on specific dystopian conventions in Holden's and MacLennan's respective novels. Then, this study looks at how Atwood's critique of nature as a victim in Oryx and Crake and The Handmaid's Taie engages with ecocritic and ecofeminist ideas causing a breakdown in the generic conventions of Atwood's dystopian novels. / Cette thèse explore le rôle et la représentation de la nature dans les romans dystopiques canadiens suivants: After the Fact (1986) d'Hélène Holden, Voices in Time (1986) de Hugh MacLennan, Oryx andCrake (2004) et The Handmaid's Taie (1985) de Margaret Atwood. Ayant pour objectif de démontrer que la fiction canadienne dystopique se distingue de la littérature dystopique traditionnelle en fonction de son utilisation dominante de la nature, cette thèse examine l'influence des images, des symboles et des archétypes de la nature sur les conventions dystopiques spécifiques à After the Fact de Holden et Voices in Time de MacLennan. Ensuite, cette étude analyse la représentation de la nature comme victime dans Oryx and Crake et dans The Handmaid's Taie de Atwood qui diffère des conventions traditionnelles du roman dystopique en s'inspirant des idées découlant de l'écoféminisme et de l'écocritique.

The screen, as recent studies in a number of fields indicate, is a cultural object due for critical reappraisal. Work on the theoretical status of screen objects tends to focus upon the materialisation of surface; in other words, it attempts to rethink the relationship between the supposedly 'superficial' facade and the 'functional' object itself. I suggest that this work, while usefully chipping away at the dichotomy between the 'superficial' and the 'functional', can lead us to a more radical conclusion when read in the context of subjectivity. By rethinking the relationship between the surface and the obverse face of the screen as the terms of a dialectic, we can ‘read’ the screen as the vital component in a process which constitutes the Subject. In order to demonstrate this, I analyse productions of subjectivity in literary texts of the twenty-first century — in doing so, I assume the novel as nonpareil arena of the dramatisation of subjectivity — and I propose a reading of the work of Jacques Lacan as hitherto unacknowledged theorist par excellence of the form and function of the screen. Lacan describes, with the function of desire and the formation of the screen of fantasy, the primary position this ‘screen-form' inhabits in the constitution of the Subject. Lacan’s work forms a critical juncture through which we must proceed if we are to properly read and understand the chosen texts: The Book of Strange New Things by Michel Faber; The Tain by China Miéville; Oryx and Crake by Margaret Atwood; and Austerlitz by W.G. Sebald. In each text, I analyse the particular materialisations of the screen and interrogate the constitution of the subject and the locus of desire. By analysing the vicissitudes of subjectivity in these texts, I make a claim for the study of the screen as constituting a central question in the field of contemporary literature.