Diss. (sammanfattning) Umeå : Umeå universitet, 2007. / Härtill 5 uppsatser.

Thesis (Ph. D.)--University of Virginia, 2008. / Title from title page. Includes bibliographical references. Also available online through Digital Dissertations.

Thesis (M.S. in Astronautical Engineering)--Naval Postgraduate School, Dec. 2004. / Thesis Advisor(s): Sherif Michael. Includes bibliographical references. Also available online.

Thesis (Ph.D.)--University of Alabama at Birmingham, 2010. / Title from PDF title page (viewed on June 28, 2010). Includes bibliographical references.

Thesis (Ph. D.)--University of Missouri-Columbia, 2006. / "December 2006" The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. Includes bibliographical references.

Thesis (M.S.)--Uniformed Services University of the Health Sciences, 2006 / Typescript (photocopy)

Space technologies are becoming increasingly indispensible for modern societies and militaries around the globe, through a heightened dependency on satellite based services. At the same time, anti-satellite weapons are being developed and tested with the capacity to attack and destroy a satellite with precision. In the light of this development, the legal uncertainty on what rules apply to belligerent use of force in the realm of outer space is problematic. Since dual-use satellites do not enjoy explicit special protection in international law, it is relevant to clarify what legal protection they do hold, being objects enabling indispensable societal services. Would state practice and hostile space conduct prevail clarification, a fatal international custom might develop, and the difficulty of reaching agreements regulating attacks on satellites, along with other hostile behavior, enhanced. The purpose of this thesis is to investigate what the current frameworks of space law and international humanitarian law provide in terms of restraints to states’ legal possibilities to attack dual-use satellites in times of armed conflict. It is concluded that both frameworks do hold restrictions respectively. Though, the need for regulation is still evident in order to achieve a clear and foreseeable legal reality along with a sustainable future of space exploration where international peace and security can be maintained.

The purpose of this study was to clarify the ambiguity in the law relating to the extended continental shelf in Article 76 of the 1982 United Nations Convention on the Law of the Sea. Another aim was to study the application of the law in a more focused part of the world, the region of East Asia, and in particular, Malaysia. The study also sought to propose solutions to issues relating to the extended continental shelf. The history of the law relating to the continental shelf, the codification of the law, and the enforcement of the law by the Commission on the Limits of the Continental Shelf is presented. Besides that, Article 76 was also thoroughly discussed in order to identify the problems involved. Besides that, the two biggest issues which determine the outer limits of the continental shelf are examined. These are issues relating to ridges and submarine elevations and the application of the foot of continental slope provisions. The study examined the problems involved with the legal and scientific interface found in Article 76 and addressed them by referring to the legislative history of Article 76, State practice and the practice of the Commission. The continental shelf in the East Asian region is also analysed in order to provide an overview of the continental shelf issues in the region. Special reference to Malaysia is made as a State that has made a submission on its outer limits of the continental shelf. A thorough analysis was made based on the findings made in this study. This study also explored possible solutions to the continental shelf issues discussed.

Hygienic care is one of the most critical pillars of care for child´s health. Learning the right hygienic habits in childhood and keeping them throughout the lifetime is of vital importance for human health. Unfortunately, information about the hygiene of external genital of children is often inconsistent, both among the parents and healthcare workers. The thesis deals with the issue of treatment of external genital of children from birth to three years of age, while the main emphasis is put on education of mothers. The objective of the thesis was to determine, whether the nurses know their competences in treatment of the external genital in newborns, babies and toddlers and whether the nurses are able to apply the competences in full. The third objective of the thesis was to map the awareness of parents of how to treat external genital of children from birth to three years of age. Three hypotheses were established. The first hypothesis: Nurses know their competences in treatment of external genitals of newborns, babies and toddlers. The second hypothesis: Nurses, who know their competences in treatment of external genital of newborns, babies and toddlers, are unable to fully apply them. The third hypothesis: Parents of newborns, babies and toddlers do not have sufficient information about the proper hygienic treatment of external genital of their children. The quantitative research used two anonymous questionnaires, while one questionnaire was designed for nurses working with children from birth to 3 years of age. The other questionnaire was created and intended for mothers of children in the age category from birth to three years. The questionnaire research was performed on the territory of the South Bohemian region. The first hypothesis: ``Nurses know their competences in treatment of external genital of newborns, babies and toddlers.{\crq}q has been confirmed. The second hypothesis: ``Nurses, who know their competences in treatment of external genital of newborns, babies and toddlers in treatment of external genital of newborns, babies and toddlers, are unable to fully apply them.{\crq}q has been rebutted. The third hypothesis: ``Parents of newborns, babies and toddlers do not have sufficient information about the proper hygienic treatment of external genital of their children.{\crq}q has been confirmed. Based on those findings we have decided to develop an educational standard for nurses about the intimate hygiene of girls and boys from birth to three years of age and an educational material for treatment of external genital for parents of young children. We believe that the materials will improve the awareness of this issue and result in a better quality of care for children.

Orientador: Marcelo Lancellotti / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-21T23:54:54Z (GMT). No. of bitstreams: 1 IzidoroJunior_MarioServulo_M.pdf: 2227378 bytes, checksum: fe650bf0745300591928bae4fac67e46 (MD5) Previous issue date: 2013 / Resumo: Embora comensal da nasofaringe humana, algumas linhagens de Neisseria meningitidis ocasionalmente ultrapassam a mucosa respiratória e a barreira hematoencefálica causando doenças como meningite e septicemia. Sabe-se também que bactérias em geral possuem taxia por células cancerígenas, isto é, possuem uma predileção por infectar células tumorais. Desta forma, neste trabalho produzimos OMV de diversas linhagens de Neisseria meningitidis e analisamos as principais características físico-químicas destas vesículas e, além disso, também verificamos as principais interações desta micropartícula com a linhagem celular NG97, uma linhagem de glioblastoma, tecido pelo qual tal bactéria é conhecida por infectar em casos patológicos, por testes morfológicos in vitro e também analisamos as citocinas inflamatórias através de quantificação relativa por Real time PCR. O grande achado deste trabalho é que a utilização de ultrafiltração substituindo a ultracentrifugação na produção de OMVs é viável, menos trabalhosa e mais rápida que os métodos descritos na literatura. Além disso, as vesículas extraídas por tal processo apresentam tamanhas e cargas superficiais como os encontrados na literatura sendo que cada cepa produz diferentes quantidades de OMVs para um mesmo tempo. Ainda, observou-se que as OMVs obtidas de linhagens atenuadas para os fatores de virulência causaram menores alterações morfológicas na linhagem NG97 do que as OMVs obtidas a partir de linhagens selvagens. A influência de tais fatores de virulência, que auxiliam na interação das células do parasita com as células do hospedeiro, também foi observada na análise das citocinas inflamatórias produzidas, onde foram constatados que na ausência da pilina (proteína responsável pela aderência da bactéria com células epiteliais e endoteliais) a produção de citocinas foi praticamente nula quando comparada com suas diferentes linhagens onde este fator de virulência é presente. Para um possível uso destas vesículas como sistemas lipossomais de entrega de drogas, acredita-se que quanto mais inócua a vesícula for maior seria a biodisponibilidade da molécula antitumoral a ser entregue, como é o caso da OMV produzida pela cepa C2135 ?pilE que, além disso, possui um tamanho menor de 200nm sendo capaz de penetrar nas junções celulares da massa tumoral / Abstract: Neisseria meningitidis is a human nasopharyngeal commensal, however, some strains occasionally exceed the respiratory mucosa and the blood brain barrier causing diseases such as meningitis and septicemia. It is well established that bacteria has a natural predilection to infect cancer cells. Thus, this study produced OMVs from different strains of Neisseria meningitidis and analyzed physicochemical characteristics of these vesicles such as size and surface charge, and also, the main interactions between this nanoparticle and NG97 cell line by in vitro morphological tests and the analyze of the production of inflammatory cytokines by relative quantification Real time PCR. The major finding of this study is that the use of ultrafiltration substituting ultracentrifugation on OMVs production makes the extraction viable, less laborous and faster than previous methods. Furthermore, the vesicles extracted by this process exhibited similar sizes and surface charges when compared to the literature and each strain produces different amounts of OMVs when comparing the same production time. In this work, we also observed that the OMVs generated by attenuated strains for virulence factors induced less morphological changes in the NG97 cells than OMVs generated from wild type strains. This trend where virulence factors which assist the parasite-host cells interaction was also observed in the analysis of cytokines produced. It was observed in the absence of pilin (protein responsible for adherence of the bacteria to epithelial and endothelial cells) the production of cytokines was much lower when compared with the different strains where this virulence factor is present. For a possible use of these lipossomal vesicles as delivery systems for drugs, it is believed that the more innocuous the greater the bioavailability of the antitumor molecule to be delivered, as in the case of OMV produced by the strain C2135 ?pilE, moreover, having a size around 200 nm fits perfectly between the cell junctions allowing the nanoparticle to reach the tumor mass, but further studies are required to confirm this hypothesis / Mestrado / Bioquimica / Mestre em Biologia Funcional e Molecular