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Cellular level/distribution of -secretase subunit nicastrin and its modulator p23 in the brainKodam, Anitha 06 1900 (has links)
The processing of amyloid precursor protein (APP) by - and -secretases produces amyloid (A) peptide, the principal component of the neuritic plaques found in Alzheimers disease (AD) pathology. The enzyme -secretase is a
multimeric protein consisting of presenilins-1/2 (PS1/PS2), nicastrin, anterior pharynx defective 1 (APH-1) and presenilin enhancer-2 (PEN-2). Recently it was
discovered that p23, a transmembrane protein involved in intracellular protein trafficking, negatively regulates -secretase activity. In the present study, I evaluated the levels/expression of the nicastrin and p23 and their possible colocalization with PS1 in normal adult and developing brains. Additionally, I have studied the alterations of p23 levels in both animal model of
neurodegeneration and in postmortem AD brains. Nicastrin and p23 were widely distributed throughout the brain and colocalized in all brain regions with PS1. The levels of nicastrin and p23 were relatively high at the early stages of postnatal development and then declined gradually as age increased. Interestingly, p23 level/expression was found to be altered following kainic acid-induced neurodegeneration in the adult rat brain. Additionally, p23 levels were reduced in the brains of individuals with AD. These results, taken together, suggest that both nicastrin and p23 are expressed in neurons throughout the brain and their levels decline gradually during development to reach an adult profile. Additionally, my results indicate that a decreased level of p23 may contribute to AD pathogenesis
by increasing the production of A-related peptides.
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Cellular level/distribution of γ-secretase subunit nicastrin and its modulator p23 in the brainKodam, Anitha Unknown Date
No description available.
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Analysis of the interaction between the co-chaperone p23 and the aryl hydrocarbon receptorThompson, John D. 01 January 2015 (has links)
The aryl hydrocarbon receptor (AhR) carboxyl terminal transcriptional activation domain was cloned, purified in denatured conditions from bacteria, refolded via limited dialysis, and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 transactivation domain construct was used, along with amino terminal AhR deletion constructs AhR CΔ274 and AhR CΔ553, to attempt to elucidate the nature of the interaction between AhR and p23 in vitro.
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National and sectoral factors in wage formation in Central and Eastern EuropeStockhammer, Engelbert, Onaran, Özlem January 2006 (has links) (PDF)
The paper investigates the formation of wages in the New Member States in Central and Eastern Europe, in particular the question what the relative role of national and sectoral factors is. While the labor relations in these countries are still in the process of change, some pattern and national differences have emerged. The question is thus to what extent these differences in labor relations are reflected in wage formation. The literature on Western OECD economies is unanimous that coordination of wage bargaining does reduce the wage spread, but disagrees on its effects on unemployment and inflation. The paper analyses wage formation in Slovenia, Slovakia, Hungary, Poland, the Czech Republic and Lithuania by means of a panel analysis for manufacturing sectors. The average wage (in the total economy) serves as a national factor and sectoral productivity serves as a sectoral factor. In variations of the basic estimation equation the role of FDI and openness and of capital intensity and skill are also discussed. The results between countries are compared with the recent index of the coordination of collective bargaining by Visser (2005) and with cross country data on union density. (author's abstract) / Series: Department of Economics Working Paper Series
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Estudo funcional comparativo das co-chaperonas moleculares p23A e p23B da Hsp90 de Leishmania braziliensisAlmeida, Glessler Silva 03 June 2014 (has links)
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Previous issue date: 2014-06-03 / Universidade Federal de Sao Carlos / Protein folding is essential for proteins proper biological function. Failures in this process can lead to the formation of poorly unfolded proteins and/or protein aggregates. In order to avoid this problem, the cells express a family of proteins known as molecular chaperones. The molecular chaperones are proteins that assist the correct folding of other proteins, and other important functions in the cells. The Hsp90 family is important for protein folding and it assists in preventing protein aggregation. Hsp90 is regulated by several co-chaperones, for example, p23. The p23 is a small acidic protein that regulates the ATPase activity of Hsp90. It has a structured N-terminal beta-sheet and an unstructured C-terminal domain. In addition to the regulatory role, as an inhibitor of ATPase activity of Hsp90, it also has chaperone activity in itself. Thus, the aim of this study was to investigate comparatively two p23 identified in the Leishmania braziliensis (Lbp23A and Lbp23B) genome. The proteins were expressed, purified and structurally and functionally characterized. Furthermore, functional assays such as intrinsic chaperone activity and inhibition of ATPase activity of Hsp90 L. braziliensis (LbHsp90) and identifying in vivo by western blotting were developed. The results indicate that these two proteins are structurally similar, however, demonstrated significant differences in chemical and thermal stability. The Lbp23 also differ in relation to chaperone activity and inhibition of ATPase activity of LbHsp90. The in vivo identification revealed the presence of both Lbp23 in extracts of L. braziliensis; besides suggesting possible post-translational modifications in Lbp23B. The results indicate that both Lbp23 are undoubtedly p23, since they show p23-like function and structural signs. / O enovelamento proteico é essencial para a correta função biológica das proteínas. Falhas nesse processo podem levar à formação de proteínas mal enoveladas e/ou agregados proteicos. Para tentar evitar esse problema, a célula expressa uma família de proteínas denominadas de chaperonas moleculares ou proteínas de choque térmico (Hsp). As chaperonas moleculares auxiliam no enovelamento correto de outras proteínas, entre outras funções importantes para as células. A família das Hsp90 são chaperonas importantes por auxiliarem no enovelamento proteico e prevenirem a agregação de proteínas. A Hsp90 é regulada por diversas co-chaperonas, como, por exemplo, a p23. A p23 é uma pequena proteína ácida que regula a atividade ATPásica da Hsp90. Ela possui um domínio N-terminal estruturado em folhas-beta e um domínio C-terminal desestruturado. Além do papel regulatório, inibindo a atividade ATPásica da Hsp90, ela possui atividade chaperona. Desta forma, o objetivo desse trabalho foi estudar comparativamente as duas p23 identificadas no genoma do protozoário Leishmania braziliensis (Lbp23A e Lbp23B). As proteínas foram expressas, purificadas e caracterizadas estrutural e funcionalmente. Além disso, foram desenvolvidos experimentos funcionais como: atividade chaperona; inibição da atividade ATPásica da Hsp90 de L. braziliensis (LbHsp90) e identificação in vivo por western blotting. Os resultados indicam que essas duas proteínas são similares estruturalmente, porém, possuem estabilidade química e térmica notavelmente diferente. Ambas Lbp23 apresentam diferenças em relação à atividade chaperona e inibição da atividade ATPásica da LbHsp90. A identificação in vivo mostrou a presença das duas Lbp23 em extratos de L. braziliensis; além de sugerir possíveis modificações pós-traducionais na Lbp23B. Os resultados indicam que ambas as Lbp23 de L. braziliensis são inequivocamente p23, pois possuem sinais estruturais e função desta co-chaperona.
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Mechanistic Study of p23-Mediated Aryl Hydrocarbon Receptor ExpressionPappas, Beverly 01 January 2018 (has links)
The aryl hydrocarbon receptor (AHR) is a ligand-activated signaling molecule which is involved in diverse biological functions ranging from cancer metastasis to immune regulation. This receptor forms a cytoplasmic complex with Hsp90, p23, and XAP2. We have previously reported that down-regulation of p23 triggers degradation of the AHR protein, uncovering a potentially dynamic event which controls the cellular AHR levels without ligand treatment. Here we investigate the underlying mechanisms for this p23 effect using wild-type HeLa and the p23 knockdown HeLa cells. Reduction of the Hsp90 and XAP2 contents, however, did not affect the AHR protein levels, implying that this p23 effect on AHR is more than just alteration of the cytoplasmic complex dynamics. Association of p23 with Hsp90 is not important for the modulation of the AHR levels since exogenous expression of p23 mutants with modest Hsp90-binding affinity effectively restored the AHR message and protein levels. The protein folding property of p23 which resides at the terminal 50-amino acid region is not involved for this p23 effect. Results from our interaction study using the affinity purified thioredoxin fusion proteins and GST fusion proteins and isothermal titration calorimetry showed that p23 directly interacts with AHR and the interaction surface lies within AHR amino acid 1–216 and p23 amino acid 1–110. Down-regulation of the p23 protein content promotes the ubiquitination of AHR, indicating that p23 protects AHR from the ubiquitin-meditated protein degradation. However, the increased ubiquitination is not through the small ubiquitin-like modifier (SUMO) signaling pathway.
Troubleshooting and optimization were paramount for understanding and evaluating the p23 and AHR interaction. Specifically, the p23 mutant purification, p23: Hsp90 interaction, transient transfection, p23: AHR assay, and ITC study were phases of this research that required extensive time and critical thinking. These topics were further detailed to outline the specific problems encountered and the various steps taken to alleviate or optimize these issues.
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REGULATION OF INTRACELLULAR ARYL HYDROCARBON RECEPTOR PROTEIN LEVELSChen, Jinyun 01 January 2020 (has links)
The aryl hydrocarbon receptor (AHR) is a ligand-activated signaling molecule which controls tumor growth and metastasis, T cell differentiation, and liver development. Expression levels of this receptor protein are sensitive to the cellular p23 protein levels in immortalized cancer cell lines. As little as 30% reduction of the p23 cellular content can suppress the AHR function. Here we reported that down-regulation of the p23 protein content in normal, untransformed human bronchial/tracheal epithelial cells to 48% of its content also suppresses the AHR protein levels to 54% of its content. This p23-mediated suppression of AHR is responsible for the repression of (1) the ligand-dependent induction of the cyp1a1 gene transcription; (2) the benzo[a]pyrene- or cigarette smoke condensate-induced CYP1A1 enzyme activity, and (3) the benzo[a]pyrene and cigarette smoke condensate-mediated production of reactive oxygen species. Reduction of the p23 content does not alter expression of oxidative stress genes or production of PGE2. Down-regulation of p23 suppresses the AHR protein levels in two other untransformed cell types, namely human breast MCF-10A and mouse immune regulatory Tr1 cells. Collectively, down-regulation of p23 suppresses the AHR protein levels in normal and untransformed cells and can in principle protect our lung epithelial cells from AHR-dependent oxidative damage caused by exposure to agents from environment and cigarette smoking.
The AHR is expressed in triple-negative and non-triple-negative breast cancer cells. It affects breast cancer growth and crosstalk with the estrogen receptor signaling. Normally the AHR is degraded shortly after ligand activation via the action of 26S proteasome. Here we report that the piperazinylpyrimidine compound Q18 triggers AHR protein degradation which is mediated through chaperone-mediated autophagy in triple-negative breast cancer cells (MDA-MB-468 and MDA-MB-231). This lysosomal degradation of AHR exhibits the following characteristics: (1) not observed in non-triple-negative breast cancer cells (MCF-7, T47D, and MDA-MB-361); (2) inhibited by progesterone receptor B but not estrogen receptor alpha; (3) reversed by chloroquine but not MG132; (4) required LAMP2A; (5) triggered by 6 amino-nicotinamide and starvation and (6) involved AHR-LAMP2A interaction mediated by 6 amino-nicotinamide and starvation. The NEKFF sequence localized at amino acid 558 of human AHR is a KFERQ-like motif of chaperone-mediated autophagy, essential for the LAMP2A-mediated AHR protein degradation.
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Mechanistic studies on protein factors dependent formation of the aryl hydrocarbon receptor -DNA complexShetty, Premnath Vithal 01 January 2003 (has links) (PDF)
Dioxins and several halogenated polycyclic aromatic hydrocarbons belong to a class of toxic environmental pollutants that give rise to a myriad of teratogenic and carcinogenic responses and are of major concern from a human health perspective due to their widespread distribution. Apart from an array of toxic endpoints, they affect the expression of a variety of xenobiotic metabolizing enzymes including CYP1A1 and 1A2. Data generated by rodent studies have shown that most, if not all, of their biological and toxic effects are mediated through binding to the aryl hydrocarbon receptor (AhR). Upon ligand binding, AhR translocates into the nucleus and heterodimerizes with AhR-nuclear translocator (Arnt); the heterodimer binds to the dioxin response element (DRE) located upstream to the promoter region of target genes, leading to their transcription. The AhR/Arnt/DRE complex has been well characterized and can be observed readily by the gel shift assay. However, the mechanism for this AhR complex formation is unclear. Baculovirus expressed, metal resin-purified human AhR and Arnt are unable to bind the DRE in a ligand-dependent manner unless crude extracts, such as the rabbit reticulocyte lysate (RRL), are reconstituted with these proteins. Proteins in the RRL are responsible for this restoration of the gel shift complex because the activity is sensitive to both heat and proteolytic treatments. Fractionation of the RRL using centricon devices gave the enriched activity in the C10 retentate fraction (C10R). Screening gel shift assays and immunodepletion studies showed that p23 and CyP40, but not hsp90 and hsp70, could be the protein factors. Purified bacterial expressed p23 restored the gel shift complex; and the mechanism is mediated at the heterodimerization step and is hsp90-dependent. However, p23 is not the major factor since the same amount of C10R as that of purified p23 produced a much more pronounced gel shift activity and was insensitive to geldanamycin and apyrase. CyP40 is unable to restore the complex formation directly; however, our data suggested that some of the CyP40-interacting proteins restore the AhR/Arnt/DRE complex formation.
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POST-TRANSLATIONAL MODIFICATION AND DEGRADATION MECHANISMS OF THE ARYL HYDROCARBON RECEPTORYang, Yujie 01 January 2021 (has links)
The aryl hydrocarbon receptor (AHR) is a transcription factor first discovered to be activated by exogenous ligands, such as dioxins, and helps promote downstream gene (e.g. CYP1A1) transcription to metabolize the toxicants. With the reports of various AHR targets genes, the expression levels and activities of AHR have been implicated in many physiological and pathological situations. Understanding how AHR protein level is regulated would provide more information to target AHR. AHR stays in the cytosol in the absence of ligand in a complex with HSP90, p23 and XAP2. After ligand activation, AHR translocates into the nucleus, fulfilling its transactivation function and then is finally degraded by proteasomes. Here, we discovered a new mechanism that controls basal AHR protein level: the selective autophagy. Loss of AHR co-chaperone p23 leads to increased protein degradation of AHR through autophagy in HeLa cells. Inhibition of autophagy using several inhibitors (chloroquine, bafilomycin A1 or 3-methyladenine) increased AHR protein levels. Knocking down of key macroautophagy protein LC3B increases AHR protein levels and decreases the responsiveness of AHR to CQ treatment. The interaction between AHR and LC3B as well as AHR and autophagy receptor p62 were confirmed in vitro and in situ. AHR is found to be lysine (K) 63-ubiquitinated in HeLa cells, which is a common signal for the autophagy-lysosomal degradation.6 We also discovered that AHR is controlled by glycogen synthase kinase 3β (GSK3β) phosphorylation. Inhibition of GSK3β activity or its expression level increased AHR protein levels while expression of HA tagged-GSK3β lowers AHR protein levels. AHR protein level is regulated through autophagy. We confirmed the GSK3β-mediated phosphorylation of AHR by phos-tag gel electrophoresis couples with Western blot analysis and identified three putative phosphorylation sites of AHR in the C-terminal half of AHR sequence. Moreover, phosphorylated AHR constitutes the active pool for transactivation and phosphorylation tagged AHR for the autophagy-lysosomal degradation, which may act as way to limit its function.
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