Thesis (Ph. D.)--University of Wisconsin--Madison, 1948. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 99-100).

Thesis (M.S.)--University of Wisconsin--Madison, 1941. / Typescript. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 44-48).

Thesis (M. Sc.)-- Springfield College, 2005. / Bibliography: leaves 89-94.

Thesis (M.S.)--Springfield College, 2005. / Includes bibliographical references. Also available online (PDF file) by a subscription to the set or by purchasing the individual file.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1942. / Part I.A. reprinted from Journal of biological chemistry, vol. 137, no. 1 (Jan. 1941), p. 363-372. Part II. reprinted from Industrial and engineering chemistry, Analytical edition, vol. 13 (15 Aug. 1941), p. 566-570. Remainder typescript. Includes abstract and vita. Includes bibliographical references.

Information on human needs for pantothenic acid is limited and no recommended daily allowance has been established; although a safe and adequate level of 4-7 mg/day has been suggested for adults and adolescents. The adolescent population is often at risk for nutritional problems because of unusual eating patterns and major physiological changes which occur at this time. Pantothenic acid levels in urine, whole-blood and erythrocytes were determined in a healthy adolescent population using radioimmunoassay techniques. Dietary intakes were calculated from 4 day diet records and evaluated using a computer data base developed at Utah State University in conjunction with USDA. Forty-nine percent of the females and 15 percent of the males consumed less than 4 mg/day; however average blood levels for both males and females were in a 11normal11 range relative to other populations {411.9 ± 102.8 ng/ml and 344.5 ± 113.6 ng/ml, respectively). Dietary intake was highly correlated to urinary excretion (p < 0.001). Levels of pantothenic acid in erythrocytes correlated well to dietary intake and urinary excretion. A model was developed to predict circulating levels of pantothenic acid from dietary intake and urinary excretion.

Thesis (MSc (Chemistry and Polymer Science))--University of Stellenbosch, 2006. / This work focuses on the biocatalytic production of coenzyme A (CoA) analogues with different tether lengths in its pantetheine moiety, and on analogues where the cysteamine moiety has been replaced with a range of other amines. An attempt was made to develop a simple biocatalytic method for the optimum production of such CoA analogues by chemo-enzymatic means. Pantothenic acid ethyl thioesters with different tether lengths were first synthesized as substrates of the CoA biosynthetic enzymes, CoaA, CoaD and CoaE. The acceptability of these compounds as substrates for the pantothenate kinase (CoaA) from prokaryotic and eukaryotic organisms was investigated through kinetic studies. These substrates were subsequently exposed to CoaA, CoaD and CoaE to produce various general CoA synthons (ethyl pre-CoAs). Finally aminolysis of these ethyl pre-CoAs by cysteamine and homocysteamine gave the various CoA analogues of different tether lengths in their pantetheine moiety. The identical production of a second type of CoA synthon (phenyl pre-CoA) from pantothenic acid phenyl thioesters was also investigated as a means to increase reactivity of the thioester substrates. Aminolysis of the phenyl pre-CoA produced the corresponding CoA derivative, but reactivity was lower than expected. A second strategy was also developed where the pantothenic acid phenyl thioesters were first aminolyzed, resulting in various pantothenamide intermediates. Aminolysis was attempted with thiol-bearing amines such as cysteamine and homocysteamine as well as with amines without sulfhydryl functionalities. These pantothenamide intermediates were then used in the biosynthesis of the corresponding CoA analogues by addition of CoaA, CoaD and CoaE. The ideal method of CoA analogue production will utilize a continuous bioreactor system in which these analogues can be prepared on large scale. However, to construct a bioreactor the enzymes involved need to be immobilized on a matrix in order to transform substrate to product. The enzymes CoaA, CoaD and CoaE can be immobilized on cellulose via a cellulose binding domain (CBD) affinity tag. Various types of CBDs were investigated and used in the construction of suitable expression vectors. Optimum expression conditions to obtain soluble CBD-fused enzymes were developed.

There has been insufficient data available for suggesting a recommended dietary allowance for pantothenic acid in the elderly population. The purpose of this study was to determine the average dietary intake of pantothenic acid and correlate it with urinary excretions and the blood levels of this vitamin to assess the pantothenic acid status of the elderly population. A seven day diet record was obtained from 65 non-institutionalized and 26 institutionalized persons over 65 years of age assessed to be free of chronic diseases. A 24 hour urine specimen and a fasting blood sample was obtained from each study participant. An average of the seven day diet record was calculated to obtain the amount of pantothenic acid consumed per day. The averaged value calculated for dietary in-take was correlated with urinary excretion and blood levels of the vitamin. The amount of pantothenic acid ingested from vitamin supplements was also calculated. The total amount of pantothenic acid (dietary and vitamin sources) was correlated with urinary excretion levels and blood levels of the vitamin. The average dietary intake of pantothenic acid for the elderly population studied was 5.9 ± 1.4 mg/day. The average total intake of pantothenic acid (dietary and vitamin sources) was 10.3 ± 13.8 mg/day. The dietary intake of pantothenic acid ranged from 2.5 - 9.5 mg/day and the intake of pantothenic acid from vitamin plus dietary sources ranged from 2.5 - 122.4 mg/day. there was a low to moderate correlation between the intake of pantothenic acid and the urinary excretion of the vitamin and a low to moderate correlation between the intake levels and blood levels of pantothenic acid.

1. A brief historical introduction is given. It deals with the general features of wound healing in which epithelial participation is the dominant feature. 2. In Section A the influence of ascorbic acid on healing of skin wounds in rats is discussed. 3. Second wounds were inflicted at the same site as the initial wounds one month after the latter had healed. 4. In Section B the influence of ascorbic acid on healing of skin wounds in guinea pigs is investigated. 5. In order to discover the action ascorbic acid has on ear wounds, the tips of guinea pigs' ears were cut off. 6. The influence of ascorbic acid on the regeneration of corneal epithelium in guinea pigs is discussed. 7. The effect of ascorbic acid on healing of muco-periosteum was studied. 8. The influence of pantothenic acid on healing of skin wounds in rats was studied. 9. Pantothenic acid (Bepanthen-Salbe) ointment was applied directly to skin wounds.

Vitamins play an important role in the nutrition of man and animal. As early as 1897 Eijkman was able to demonstrate the symptoms of thiamine deficiency disease in birds. Since then, vitamins have been reguarded as one of the most essential factors in nutrition. Only small quantities in the diet are necessary, yet lack of any of them will inhibit the growth and produce certain kinds of deficiency diseases.