Archeomagnetic field models may provide important insights to the geodynamo. Here we investigate the existence and mobility of reversed flux patches (RFPs) in an archeomagnetic field model. We introduce topological algorithms to define, identify, and track RFPs. In addition, we explore the relations between RFPs and dipole changes and apply robustness tests to the RFPs. In contrast to previous definitions, patches that reside on the geographic equator are adequately identified based on our RFPs definition. Most RFPs exhibit a westward drift and migrate toward higher latitudes. Undulations of the magnetic equator and RFPs oppose the axial dipole moment (ADM). Filtered models show a tracking behavior similar to the nonfiltered model, and surprisingly new RFPs occasionally emerge. The advection and diffusion of RFPs have worked in unison to yield the decrease of the ADM at recent times. The absence of RFPs in the period 550-1440 A.D. is related to a low in intermediate degrees of the geomagnetic power spectrum. We thus hypothesize that the RFPs are strongly dependent on intermediate spherical harmonic degrees 4 and above. Filtered models and comparison with the GUFM1 model suggest that RFPs are robust features of the geomagentic field for the last millennia. / Modelos do campo arqueomagnético podem fornecer importantes informações sobre o geodínamo. Nesta dissertação, estudou-se a existência e a mobilidade de lóbulos de fluxo reverso (RFPs, em inglês) no campo arqueomagnético. Testes de robustez foram aplicados para diferentes modeles. Além disso, foram exploradas as relações entre RFPs e as variações do momento do dipolo axial (ADM, em inglês). Para tanto, foram desenvolvidos algoritmos topológicos para definir, identificar e mapear os RFPs em modelos do campo arqueomagnético (modelo CALS3k.4b e modelo GUFM1). O equador magnético foi usado para definir a polaridade ao invés do equador geográfico. Uma vez que cada ponto da grade de 1 grau x 1 grau de latitude/longitude é associado com um hemisfério magnético e uma polaridade (normal ou reversa), o passo seguinte é identificar os picos de lóbulos de fluxo reverso. No passo final de identificação um critério de intensidade é imposto para evitar lóbulos que possuem intensidade muito baixa. Para mapear lóbulos de fluxo reverso no tempo, foi codificado um algoritmo que calcula a distancia de cada lobulo para todos os lóbulos do próximo intervalo de tempo. Diferentemente das definições anteriores, lóbulos que residem no equador geográfico são adequadamente identificados com a nova definição de RFPs. A maioria dos RFPs exibe deriva para Oeste e mais de 75% deles migram em direção a altas latitudes. Ondulações do equador magnético e RFPs resultam de campo com momento oposto ao ADM. Modelos filtrados apresentam resultados de mapeamento semelhantes aos de modelos não-filtrados, e em alguns casos, surgem nos modelos filtrados novos RFPs. Resultados dos modelos CALS3k.4b e GUFM1 para o período de 1840 AD até 1990 AD mostram concordância para o mapeamento de lóbulos de fluxo reverso com maiores similaridades para os filtros mais fortes. Os resultados desta dissertação são compatíveis com a ideia que a advecção e a difusão de RFPs tem operado em conjunto causando o decréscimo do ADM nos últimos séculos. A ausência de RFPs no período de 550-1440 AD sendo relacionada a baixos valores nos graus intermediários do espectro de potencia. Dessa forma, sugere-se que os RFPs são fortemente dependentes dos harmônicos esféricos de graus 4 e acima. Modelos filtrados e comparações com o modelo GUFM1 sugerem que lóbulos de fluxo reverso são feições robustas do campo magnético terrestre nos últimos milênios.

In this dissertation I first examine the ability of individual plants in the field to garner localized soil nutrients. I then measure actual soil variability around perennial plants and use various statistics to quantify the scale and degree of that variability. Soil patches on opposite sides of Pseudoroegneria spicata tussocks were treated with distilled water or a nutrient solution containing N, P, or K in three field experiments. When P was augmented in the enriched soil patches, rates of P uptake increased significantly for roots from enriched patches compared with roots in control patches. Rates of ammonium and potassium uptake were apparently unchanged. When N was augmented in the enriched patches, rates of ammonium and potassium uptake increased significantly. When K was augmented in the enriched patches, no changes were seen for any of the nutrients. Plant shading was found to limit the ability of Agropyron desertorum tussocks to increase rates of nutrient uptake in enriched soil microsites. Roots of unshaded plants selectively increased phosphate uptake capacity in enriched patches by up to 73%, but shading limited this response. Enrichment of the soil patches resulted in significantly greater phosphate concentrations in roots of both shaded and unshaded plants. Nutrient heterogeneity in the soil at a native sagebrush-steppe site was quite high, with ammonium and nitrate varying by over two orders of magnitude and phosphate and potassium close to one order of magnitude within a 10x12- m area. Within 0.5x0.5-m subplots around individual plants, ammonium and nitrate varied by an average factor of 11 and 12, respectively, with less average variation for phosphate and potassium. Geostatistical semivariograms showed that soil ammonium, nitrate, phosphate, potassium, pH, and organic matter all showed detectable autocorrelation only at scales of less than 1.0 to 1.5 m. Indices of microbial activity showed no detectable autocorrelation even at the smallest measurement scale of 12.5 cm. From the degree and scale of heterogeneity encountered, I conclude that root plasticity and active foraging in a heterogeneous soil environment are likely to be important to the nutrient balance of many plants.

This thesis was funded by the Department of Human Services (South Australia) to test the acceptability of nicotine patches to pregnant women and to assess the safety of nicotine patches for pregnant women, at least in terms of overall exposure to nicotine. The study was conducted in the antenatal clinics at the Women's and Children's Hospital, Adelaide and was a pilot for a planned larger study. If the pilot indicated that the nicotine patches could be used safely by this group of women, the larger study would examine the effectivemess of patches in a smoking cessation program. Four focus groups, three with pregnant women and one with their care providers, were used to elucidate issues for pregnant women related to smoking and the use of nicotine patches to aid cessation.

Clearance of Eucalyptus woodlands has resulted in soil deterioration and lost agricultural production, due to wind erosion, salinity and soil acidity. Despite increasing efforts to reverse these trends through Landcare and other revegetation and agroforestry programs, there is a lack of experimentally-based information about the effects of trees on native pasture performance. The study was carried out in a temperate environment (Southern Tablelands, New South Wales). The altitude at the study sites ranged from 740 to 880m and the aspect at the experimental plots varied from SE to SW. The nearest site was 16 km from Canberra Airport and all sites were situated within similar rainfall isohyets as Canberra Airport. Thus climatic conditions were expected to be similar. Climate records at Canberra Airport indicate that January is the hottest month with mean maximum temperature of 27.7 �C and July is the coldest month with a maximum of 11.1 �C. Rainfall in the area ranges from 37.5 to 66.0 mm monthly average in June and October respectively. The main tree species in the study area were Eucalyptus pauciflora, E. melliodora and E. mannifera. Furthermore, Poa labillardieri, P. sieberiana, Themeda australis, Danthonia penicillata and Microlaena stipoides were the most abundant pasture species on the experimental plots. Species of clover (Trifolium spp.) were also abundant among the herbs. This study used pasture assessment techniques to quantify the effects of remnant patches of Eucalyptus open woodlands on the composition, quality and biomass production of herbaceous understorey vegetation. Microclimate and soil nutrients were also compared under trees and in the open. In addition, consumption by vertebrate grazers under Eucalyptus trees and in the open was compared. Tree density and basal area were compared with herbage standing crop. Remnant patches of Eucalyptus open woodlands modify the microclimate by reducing wind reaching the understorey vegetation. However no significant effects on ambient air temperature and relative humidity were recorded. The effect of trees on soil moisture was contingent to differences between the four sites and soil depth. Despite a 13% higher soil organic matter in the top 15 cm of soil under trees, soil total nitrogen and total phosphorus did not differ from that in the open. Surface soil pH values were lower (by 0.2 units) under the trees. No significant effect of trees on pasture species richness was found. However the classification of quadrats on the basis of species presence showed a distinction between species composition under trees and in the open at one of the four sites. vi The contribution of pasture species to total dry weight on plots under trees and in the open did depend on the particular species involved and was also contingent to differences between sites. However at the sites where Vulpia bromoides and Poa sieberiana were abundant, the two species dominated the biomass under trees. Whereas Microlaena stipoides var. stipoides dominated the biomass under trees at two sites and in the open at only one of the four sites. Pasture total N content differed between sites. Two of the sites had significantly higher (5.9% and 19.7%) N content under trees. On the contrary, pastures at one site contained 18.7% higher N content in the open. The total P content was 18% higher in pastures under trees. Overall, the pasture standing crop under trees was 15% less than in the open during August to May. Vertebrate grazers consumed about the same amount of pasture under the trees and in the open at the four experimental sites.

<p>The use of nano- and micro-particulate carriers as delivery systems for oral vaccines has been under investigation for several decades. Surprisingly little is known of their uptake in the human intestine, despite the fact that substantial improvement is required to achieve adequate immune responses in man after oral administration. </p><p>In this thesis, various aspects of particle transcytosis across the human intestinal epithelium were studied, in order to identify strategies for improved uptake of nano- and micro-particulate drug delivery systems. </p><p>The follicle associated epithelium (FAE) overlying Peyer´s patches contains M-cells, which have an increased capacity for uptake of particulate antigens. Therefore, a model of human FAE was developed to study mechanisms of particle uptake and transport.</p><p>Receptors that could be used for targeting to the FAE had previously not been identified in humans. By use of the model FAE, two new targets were identified on human intestinal FAE; CD9 and β1-integrin. Furthermore, studies of isolated human intestinal tissue showed that an integrin-adherent peptide motif, RGD, could be utilized to achieve selective and improved transport of nanoparticles into human Peyer´s patches.</p><p>Studies of factors influencing intestinal particle uptake and transcytosis revealed that two cytokines, TNF-α and LTα1/β2, but also one growth factor, TGF-β1, induced uptake of particles in Caco-2 cells and transcytosis of particles in the model FAE. Furthermore, it was shown that an enteric bacterium, Yersinia Pseudotuberculosis, could trigger uptake and transcytosis of particles across model absorptive epithelial cells.</p><p>In conclusion, this thesis provides a platform for further investigations of particle transcytosis across the human intestinal epithelium. The identification of two new proteins with increased expression in human FAE and a targeting sequence that improves particle uptake into Peyer’s patches, gives new hope for the development of subunit oral vaccines.</p>

Recently documented ice patch sites in the southwest Yukon are ideal for evaluating precontact hunter-gatherer land-use patterns in the western subarctic. Located in the alpine of the mountainous regions of the boreal forest, ice patches are associated with well preserved hunting equipment, caribou (Rangifer tarandus) dung and an abundance of faunal remains dating to over 8000 years ago. However, current models are inadequate for explaining caribou hunting at ice patches as they tend to emphasize large-scale communal hunts associated with latitudinal movements of caribou. Much less is known about the alititudinal movment of caribou and the associated hunting forays to ice patches in the alpine. Based on literature from caribou biology an altitudinal hunting model is proposed. During summer months caribou are predictable in their use of ice patches for relief from insect harassment. Pollen dated from caribou dung frozen in organic layers from the Granger (JdUt-1) and Friday Creek (JcUu-1) ice patches was analysed and compared to pollen assemblages from modern caribou dung to test whether ancient caribou were using these locations during summer months. The multivariate statistical technique, Nonmetric Mutlidimensional Scaling shows that ancient pollen assemblages are unlike any modern dung. Results indicate that pollen derived from dung is complex and various temporal transformations and taphonomic factors such as: (i) the use of modern analogue samples; (ii) changes in phenology; (iii) mode of pollination and; (iv) caribou feeding strategies must be understood before making interpretations on seasonality from dung pollen. I propose that a qualitative model of seasonal pollen signatures also be used to evaluate ancient pollen spectra, especially when there is no modern analogue. Regardless of these factors, the identification of a diversity of forbs and the presence of insect-pollinated taxa such as Polemonium and Epilobium suggest that some of the dung was deposited by caribou in the summer. Ancient hunters, knowing that caribou aggregate in mixed herds on ice patches in summer months, took advantage of this behaviour. Hunting equipment found on ice patches indicates that atlatls (8360± 60 to 1250± 40 yrs BP) and bow and arrows (1300 ± 70 to 90 ± 40 yrs BP) and hunting blinds were part of the ice hunting strategy. Faunal analysis suggests caribou was the primary game animal hunted at ice patches, although sheep (Ovis dalli) may have been important at some locations. Developing an altitudinal migration model provides a fuller picture of caribou hunting at alpine locations in the southwest Yukon and assists in understanding Holocene precontact hunting and land-use patterns in the western subarctic.

The use of nano- and micro-particulate carriers as delivery systems for oral vaccines has been under investigation for several decades. Surprisingly little is known of their uptake in the human intestine, despite the fact that substantial improvement is required to achieve adequate immune responses in man after oral administration. In this thesis, various aspects of particle transcytosis across the human intestinal epithelium were studied, in order to identify strategies for improved uptake of nano- and micro-particulate drug delivery systems. The follicle associated epithelium (FAE) overlying Peyer´s patches contains M-cells, which have an increased capacity for uptake of particulate antigens. Therefore, a model of human FAE was developed to study mechanisms of particle uptake and transport. Receptors that could be used for targeting to the FAE had previously not been identified in humans. By use of the model FAE, two new targets were identified on human intestinal FAE; CD9 and β1-integrin. Furthermore, studies of isolated human intestinal tissue showed that an integrin-adherent peptide motif, RGD, could be utilized to achieve selective and improved transport of nanoparticles into human Peyer´s patches. Studies of factors influencing intestinal particle uptake and transcytosis revealed that two cytokines, TNF-α and LTα1/β2, but also one growth factor, TGF-β1, induced uptake of particles in Caco-2 cells and transcytosis of particles in the model FAE. Furthermore, it was shown that an enteric bacterium, Yersinia Pseudotuberculosis, could trigger uptake and transcytosis of particles across model absorptive epithelial cells. In conclusion, this thesis provides a platform for further investigations of particle transcytosis across the human intestinal epithelium. The identification of two new proteins with increased expression in human FAE and a targeting sequence that improves particle uptake into Peyer’s patches, gives new hope for the development of subunit oral vaccines.

This study investigates the morphosyntactic competence of advanced ESL learners and native speakers of English. Using the framework of the Government and Binding approach (Chomsky,1981, 1986), the study tests the predictions made by the evolved Fundamental Difference Hypothesis (Bley-Vroman, 2009), namely that the grammars of advanced L2 learners are unreliable(where reliability means converging to the L2 grammar), non-convergent to the L2 grammar, and characteristic of patches (where patches are extragrammatical principles independent of the normal syntactic processes). The participants of the study were tested on three tasks (timed grammaticality judgment task, a correction task, and a preference task). The findings of the study indicate that the difference between the morphosyntactic competence of the advanced ESL learners and that of native speakers is gradient rather than categorical.

This study investigates the morphosyntactic competence of advanced ESL learners and native speakers of English. Using the framework of the Government and Binding approach (Chomsky,1981, 1986), the study tests the predictions made by the evolved Fundamental Difference Hypothesis (Bley-Vroman, 2009), namely that the grammars of advanced L2 learners are unreliable(where reliability means converging to the L2 grammar), non-convergent to the L2 grammar, and characteristic of patches (where patches are extragrammatical principles independent of the normal syntactic processes). The participants of the study were tested on three tasks (timed grammaticality judgment task, a correction task, and a preference task). The findings of the study indicate that the difference between the morphosyntactic competence of the advanced ESL learners and that of native speakers is gradient rather than categorical.

Foredunes are formed and developed in association with vegetation. A bare sand area has been viewed as a measure of dune mobility or activity and researched in association with climate controls: particularly wind power, annual mean precipitation, and temperature, expressed in annual mean potential evapotranspiration. There has been no research that utilized the patterns of bare sand areas to classify foredune areas in coastal dune systems and investigated climate controls related to sand patch patterns, or “foredune textures” such as size, number, and distribution of sand patches Four foredune types were classified based on four landscape metrics (PLAND: percentage of bare sand area, PLADJ: proportion of like-adjacencies, NLSI: normalized landscape shape index, and ENN_RA: range of Euclidean nearest neighbor), by applying the concepts and methodologies of landscape ecology. Four climate variables (annual mean precipitation, annual mean potential evapotranspiration, Lancaster’s mobility index, and the standard deviation of annual mean precipitation) were found to affect the foredune types and help in distinguishing one foredune type from another. The amount of bare sand area on coastal foredune areas can be explained by annual mean precipitation (R^2 is 0.52 at the 99 % confidence level), standard deviation of precipitation (R^2 is 0.51 at the 99 % confidence level), and Lancaster’s mobility index (R^2 is 0.37 at the 99 % confidence level) but wind variables such as drift potential do not explain much (R^2 is 0.04 at maximum). This suggests that dune activity or stabilization in coastal dune systems is mainly controlled by vegetation cover, which is in turn affected by precipitation. Foredune textures can be a useful tool to predict foredune types in association with future climate change, and the optimal averaging period of precipitation for each bare sand area was seven years.