Perennial grass vegetation dynamics of heavy grazing, moderate grazing and ungrazed treatments were analyzed during two extreme drought events: the drought of 1951 to 1956 and the drought events centered on the year 2000. Analysis of each drought event from weather records and Palmer Drought Severity Index values showed that the intensity, duration and pattern of each drought event were structurally unique. Grazing intensity was the primary driver of perennial grass species composition and community structure both during and between each drought event. Total basal area for each drought event was driven primarily by variation in precipitation pattern. Basal area per plant dynamics were significantly influenced by grazing intensity while plant density was driven by both precipitation and grazing intensity. Topo-edaphic variation significantly influenced the persistence and distribution of perennial grass species during an extreme drought event centered on the year 2000. The presence of large or abundant surface rock features facilitated the survival of perennial grasses during this drought event by creating a positive soil microenvironment. Sites with large surface rock features acted as drought refugia for perennial grass populations. Sites with abundant surface rock features had 10 times greater perennial grass basal area and 5 times greater plant density than sites with few surface rock features. Grazing intensity was the primary driver of species composition and community structure within both refugia and non-refugia sites. Grazing intensity had no effect on perennial grass basal area. However, grazed treatments had two times greater perennial grass density than non-grazed treatments. Species response to grazing intensity and surface rock cover was individualistic. Grazing response groups (intolerant, intermediate, and tolerant) separated along a gradient of surface rock cover and grazing intensity. Abundant surface rock features act as refugia for perennial grasses by facilitating their persistence during extreme drought events on the Edwards Plateau.

No description available.

Two experiments were conducted to determine the effects of grazing stockpiled perennial forage in field paddocks relative to feeding similar quality round bale hay in drylot pens on rumen degradation characteristics of forage; beef cow performance, cow reproductive efficiency, estimated dry matter intake and forage utilization, forage yield and quality, soil nutrients and system costs. Winter feeding systems were (i) stockpiled perennial forage (TDN = 58.9%; CP = 8.5%) grazing (SPF) and (ii) drylot feeding (DL) of round bale hay (TDN = 57.9%; CP = 8.4%). Experiment I was an in situ study, where five Hereford heifers (398 ± 14 kg) fitted with rumen cannulae were fed a grass hay (DM = 93.2%; TDN = 50.8%; CP = 9.8%; NDF = 66.2%) diet. In situ degradability of both stockpiled forage (SPF) and round bale hay (BH) samples collected at start (October) and end (December) of the field study were determined. The soluble fraction (S) of DM was greater (P = 0.01) in SPF October forage compared to SPF December, BH October and BH December forages. The potentially degradable fraction (D) of CP was lowest (P = 0.04) in BH December forage than in SPF October, SPF December and BH October forages suggesting that hay quality declined more rapidly than stockpiled forage and method of preservation may have affected overall hay quality. Furthermore, D fraction of both ADF and NDF was higher in SPF samples suggesting stockpiled forage may be more digestible than hay. However, the D fraction of NDF in both SPF and BH forages declined with later sampling date possibly due to effect of weathering and leaf loss. In Experiment II, 6, 4-ha paddocks consisting of meadow bromegrass (Bromus riparius Rehm) and alfalfa (Medicago sativa), were randomly assigned to 1 of 2 replicated (n = 3) winter feeding systems. In this study 58 dry pregnant (120 ± 16 d) Angus cows (675 kg ± 51 kg), stratified by body weight (BW; corrected for conceptus gain), were allocated to either the SPF or DL systems. Cows in winter feeding systems were provided additional energy supplement (rolled barley) (TDN = 86.4%; CP = 12.4%) depending on environmental conditions to maintain body condition, with no weight gain above that of conceptus growth. Dry matter intake (DMI) and forage utilization were estimated using the herbage weight disappearance method. The effects of winter feeding systems on soil nutrients were determined the following spring after winter grazing. Forage yield in DL (4683 ± 495 kg ha-1) and SPF (4032 ± 495 kg ha-1) systems was not different (P = 0.18) between treatments. However, forage utilization was lower (P < 0.01) in SPF (83.5%) than the DL (94.4%) system, signifying lower accessibility to stockpiled forage due to snow depth, lower temperatures, freezing rain and wind. Cows in the SPF system had higher forage DMI (P = 0.04) and supplementation intake (P < 0.01) compared to cows in drylot pens likely a combined effect of effective ambient temperatures below the lower critical temperature (LCT) during the grazing period and the higher potentially digestible fraction of neutral detergent fiber in stockpiled forage than hay. Cow BW change, average daily gain, rib fat change and rump fat change were not different (P > 0.05) between winter feeding systems. Reproductive performance of beef cows was not affected (P > 0.05) by either winter feeding methods as cows in both systems maintained body condition score (BCS) at 2.5 to 3.0 throughout the study. Average total production cost was 19% lower in SPF system compared to DL system. In conclusion, the rumen degradation characteristics of stockpiled perennial forages focused in this study support the utilization these forages in a winter feeding system to meet the nutrient requirements of dry beef cows in early to mid-gestation. It may be cost effective to manage beef cows in field grazing of stockpiled perennial forages in western Canada, without any negative impact on beef cow performance or reproductive efficiency.

In the United Kingdom and Ireland, a major percentage of fertilized agricultural area is devoted to grasslands, which helps to support the associated milk and beef production industries. In temperate grasslands, perennial ryegrass (L. perenne) is the major forage grass and this species is particularly suitable as a forage grass due to its high yield and digestibility, when compared with other species. However, perennial ryegrass is not well adapted to abiotic stress conditions which are likely to occur in its natural environment. Some of the abiotic stress factors which have significant impacts on plant growth and development include water and nutrient availability. Therefore, this project set out to unravel some of the mechanisms involved in the adaptation of perennial ryegrass to limited water, phosphorous and nitrogen. In order to understand the metabolic mechanisms acting in response to these stresses, metabolite profiling was performed using GC-MS. Furthermore, for the water- and phosphorous-limitation studies this approach was complemented with transcript analysis.In order to study water-limitation a hydroponics system supplemented with polyethyleneglycol (PEG) was used to induce water-limitation for a period of one-week. A clear difference in the metabolic profiles of the leaves of plants grown under water stress was observed. Differences were principally due to a reduction in fatty acid levels in the more water stress-susceptible genotype Cashel and an increase in sugars and compatible solutes in the drought-tolerant PI 462336 genotype. Sugars exhibiting a significant increase included, raffinose, trehalose, glucose, fructose and maltose. Raffinose was identified as the metabolite exhibiting the largest accumulation under water-stress in the more tolerant genotype and may represent a target for engineering superior drought tolerance or form the basis of marker-assisted breeding in perennial ryegrass. The metabolomics approach was combined with a transcriptomics approach in the water stress tolerant genotype PI 462336 which identified genes in perennial ryegrass that were regulated by this stress.The characterization of the response to phosphorus-limitation was performed in a hydroponics system containing two solutions with different levels of phosphorus. Samples were collected from the roots and leaves of two genotypes 24 hours after being exposed to stress. Internal phosphate concentrations were reduced and significant alterations were detected in the metabolome and transcriptome of two perennial ryegrass genotypes. Results indicated a replacement of phospholipids with sulfolipids in response to P deficiency and that this occurs at the very early stages of P deficiency in perennial ryegrass. Additionally, the results suggested the role of glycolytic bypasses and the re-allocation of carbohydrates in response to P deficiency The characterization of the metabolic response of L. perenne leaves to different levels of nitrogen supply was performed for seven different genotypes with variability in the regrowth response rate to nitrogen supply in a hydroponics system. This facilitated the identification of common mechanisms of response between genotypes to nitrogen. The metabolic response observed included modifications of the lipid metabolism, as well as alterations of secondary aromatic metabolite precursors in plants exposed to nitrogendeficit. In contrast, plants grown in a nitrogen saturated media appeared to modify to some extent the metabolism of ascorbate. Additionally, it was found that amino acid levels increased with increasing concentrations of nitrogen supplied. This study suggested that the involvement of secondary metabolism, together with lipid and ascorbate metabolism, is of crucial importance in the early-adaptation of perennial ryegrass plants to different levels of nitrogen supply.

Johnsongrass [Sorghum halepense (L.) Pers.] and mugwort (Artemisia vulgaris L.) are both rhizomatous perennial weeds that are capable of rapidly colonizing a variety of different environments. Separate experiments were conducted throughout Virginia from 1996 to 1999 to determine more effective methods for reducing infestations of these perennial weeds in the future. Field and greenhouse experiments conducted on a resistant johnsongrass population discovered in New Kent County, Virginia revealed that this biotype exhibits low levels of resistance to the aryloxyphenoxypropionate (APP) herbicides quizalofop-P and fluazifop-P and the cyclohexanedione (CHD) herbicide sethoxydim. Additional laboratory experiments revealed that resistance is not due to differential absorption, translocation, or metabolism of the APP and CHD herbicides in the resistant vs. the susceptible biotype. However, acetyl-coenzyme A carboxylase (ACCase) assays revealed that resistance to the APP and CHD herbicides is conferred by an overproduction of the ACCase enzyme in the resistant compared to the susceptible johnsongrass biotype. In field experiments conducted on mugwort infestations discovered in several counties throughout Virginia, 100% mugwort control was achieved with standard application rates of picloram at 4 months after treatment (MAT), and also greater than 70% mugwort control was achieved with the higher application rates of clopyralid, glyphosate, and dicamba at 4 MAT. However, all other herbicides evaluated in these experiments provided less than 65% mugwort control at 4 MAT, even at exceptionally high use rates. Additionally, the results from these trials revealed that sequential herbicide applications and sequential mowings prior to herbicide application are both effective mugwort control strategies. / Ph. D.

New hybrid Echinacea cultivars, based on crosses of Echinacea purpurea (L.) Moench with several other Echinacea species, have generated interest and excitement in the marketplace due to novel flower colors and forms. However, these cultivars vary significantly in their growth habits and requirements from the species. We examined factors in the production of Echinacea hybrid cultivars to provide guidance to growers. Foliar sprays 600 mg·L⁻¹ benzyladenine (BA) increased numbers of branches between 19% and 83% in Echinacea cultivars while 400 mg·L⁻¹ dikegulac sodium or 500 mg·L⁻¹ ethephon did not improve branching. Of several height control PGRs applied to E. ‘Marmalade,’ only plants treated with two applications of 5000 mg·L⁻¹ daminozide were shorter (24%) compared to untreated controls although flowering was also reduced by 70%. Echinacea ‘Harvest Moon’ plants were shorter in response to all of the PGRs applied, with the best results seen in plants treated with foliar sprays of uniconazole (one application of 30 mg·L⁻¹ or two applications of 15 mg·L⁻¹ ), two applications of 5000 mg·L⁻¹ daminozide, or 4 mg·L⁻¹ paclobutrazol applied once as a drench. Supplying N at 150 mg·L -1 during the growing season provided Echinacea cultivars adequate nutrition and maximized numbers of branches and flowers and shoot dry weight. In overwintering, fertilization treatments that resulted in low substrate electrical conductivity going into dormancy, 5.0 kg·m controlled release fertilizer 15N-3.9P-10K or 150 mg·L⁻¹ N using 15N-2.2P-12.5K applied using constant liquid feed, resulted in the highest survival rates of Echinacea cultivars. As a monitoring tool, SPAD measurements were not successful in predicting tissue N levels in Echinacea hybrids. Twenty-one hybrid cultivars acquired as stage 3 tissue culture plantlets were grown under one of three photoperiods (10-hour, 16-hour, or 24-hour) for 10 weeks before being transplanted to larger containers and grown under natural daylength until flowering. Providing Echinacea hybrid cultivars with a 16-hour photoperiod during liner production resulted in plants which flowered soonest without negative effects on growth. The need for height control PGRs varied by cultivar; however, overall height control PGRs controlled flower stalk height and increased market rating. / Ph. D.

Diss. (sammanfattning) Stockholm : Karol. inst., 2004. / Härtill 4 uppsatser.

For the successful reclamation of disturbed land, the reduction of initial erosion risk must be balanced with later vegetation establishment. A combination of erosion control and revegetation practices was researched using commercial (full-sized) equipment on a semi-desert grassland site in southern Arizona, USA. Two soils with different parent materials were used to add a 30 cm cap on sites at two elevations: 1646 and 1403 m asl. There were two surface roughness treatments: smooth and rough. Three straw mulch treatments were applied: no mulch, mulch incorporated into the surface soil, and mulch tackified onto the surface. Plots were planted with a 10 species native mix dominated by perennial grasses. After two growing seasons, the incorporated mulch treatment resulted in significantly more seeded grass aboveground biomass than the no mulch treatment while the no mulch treatment had more forb and volunteer biomass than the surface mulch treatment. There was significantly higher erosion on the rough surface treatment compared to the smooth surface. Increasing perennial grass biomass was correlated with reduced erosion while forb and volunteer biomass showed no relationship with erosion. The smooth surface with surface mulch best established perennial grasses, minimized weeds, and reduced erosion. This combination of practices both minimized erosion as well as maximized vegetation establishment. (C) 2016 The Authors. Published by Elsevier B.V.

Invasive species drive ecosystem changes throughout the world. Introduced grasses in dryland ecosystems have driven a grass-fire cycle that transforms ecosystems into homogenized grasslands (Brooks et al. 2004; D'Antonio & Vitousek 1992). Little is known about the spread rates of these grasses, effects on native ecosystems or how climate modulates spread, yet these uncertainties may be the difference between success and failure. Equally important is a quantitative assessment of the current states of invasion, yet mapping efforts have been lacking and remote sensing assessments have been inadequate for regional and local assessments. This research examines these uncertainties in the context of buffelgrass (Pennisetum ciliare Link (L.)), a C₄ grass introduced into the Sonoran Desert. These are presented as three distinct but related studies. The first study documents changes in diversity and dominance at 11 sites in the Sonoran Desert with respect to time since infestation by buffelgrass. Dominant and rare species alike declined rapidly following infestation, although the longer-lived shrubs showed no signs until after five years. This calls into question basic assumptions about the grass-fire cycle. The second study assesses constraints to successful operational identification of buffelgrass via remote sensing. We combined ground-based spectral measurements with cover estimates and found that Landsat TM-based classification will result in high commission/omission errors regardless of timing. We also identified several spectral characteristics that distinguish buffelgrass that are only available using hyperspectral imagery. The third study reconstructs spread of buffelgrass using historical aerial photography dating from 1979. Populations grew from small colonizing patches to 66 ha in 2008, doubling every 2-3 years since 1988. Although spread closely fit a logistic growth curve between 1989 and 2008, we found evidence that the 1980s were a period of rapid expansion. Thus, we may presently be in a period of slower spread in which treatment efforts will be more effective than the long-term average. This research documents grass-led ecosystem transformation without changes in the fire regime and constant spread rates over multiple decades. Along with suggested methods derived from our remote sensing study, this provides managers with critical information for managing buffelgrass in the long-term.

Biomass, tiller numbers, flowering, and genet survival were accessed for the rhizomatous Elymus lanceolatus ssp. lanceolatus and caespitose E. l. ssp. wawawaiensis growing in mixtures with a range of densities of each taxon. Models of aboveground biomass of each taxon as a function of mixed densities were used to calculate competition indices. Tiller numbers and biomass of ssp. lanceolatus were higher than those of the caespitose taxon in the first year, but declined in the second year, while biomass and tiller numbers of ssp. wawawaiensis changed little. All tillers of ssp. wawawaiensis emerged in autumn; tillers of ssp. lanceolatus emerged from autumn through late spring. The rhizomatous taxon better exploited open resources in the first year; ssp. wawawaiensishad slower growth, but its production of many tillers early in the season may allow it to quickly exploit seasonally variable conditions of semiarid environments. Decline in flowering at higher densities and in the second year was more pronounced than that of biomass and tiller numbers. Genet survival was high and similar for both taxa. Substitution rates indicated ssp. lanceolatus was the better competitor in both years. In the second year, the two taxa differed somewhat less in substitution rates. A greater overlap in resources used by the two taxa was indicated. Subspecies lanceolatus experienced greater intensity of competition. Substitution rates and relative efficiency index indicated ssp. lanceolatus was the greater competitor between early and late spring, when overlap in resource use was greater. Another experiment addressed advantages of clonal foraging of ssp. lanceolatus in exploiting soil nutrient patches. A foraging response was found in the rhizomatous taxon, with greater numbers of closely spaced tillers in high-nutrient patches adjacent to the main clone, but root biomass in these patches, and aboveground biomass of the clones, indicated that both taxa accessed nutrients in the patches, but ssp. wawawaiens is used only root growth. The taxa were similar in their tolerance of low levels of soil nutrients. No difference among genets in degree of foraging response, and no relationship between degree of foraging response and fitness when nutrients are patchy were found. Subspecies lanceolatus did not show such a foraging response to high neighbor densities in mixed-density plots. Instead, rhizome lengths were reduced by higher neighbor densities and in the second year, by reduced resources overall.