Orientador: Orivaldo Arf / Banca: Salatiér Buzetti / Banca: Rogério Peres Soratto / Resumo: O nitrogênio é o nutriente absorvido em maior quantidade pelo feijoeiro, sendo que o manejo da adubação nitrogenada pode interferir no desenvolvimento e na produtividade da cultura. Neste sentido, o trabalho foi desenvolvido na Fazenda Experimental da UNESP - Ilha Solteira localizada em Selvíria, Mato Grosso do Sul, com o objetivo de estudar o efeito de fontes e épocas de aplicação do nitrogênio no desenvolvimento e produtividade do feijoeiro de inverno no sistema plantio direto utilizando irrigação por aspersão em dois anos de cultivo (2006 e 2007). O solo do local é do tipo Latossolo Vermelho argiloso, a área foi cultivada nos 5 anos anteriores com milho no verão e feijão no inverno. O cultivar utilizado foi o Pérola e o delineamento experimental foi o em blocos casualizados com 16 tratamentos dispostos em esquema fatorial 2 x 8, sendo duas fontes de nitrogênio (uréia e sulfonitrato de amônio), aplicadas em sete ocasiões (30 dias antes da semeadura, 15 dias antes da semeadura, na semeadura, 15 dias após a semeadura, 30 dias após a semeadura, 1/3 na semeadura + 2/3 15 dias após a semeadura, 1/3 na semeadura + 2/3 30 dias após a semeadura) na dose de 90 kg de N ha-1, além da testemunha (sem aplicação de N). Os resultados obtidos revelaram influência das fontes na população de plantas somente no segundo ano de cultivo e a população média de plantas em cada ano de cultivo pode ter influenciado direta ou indiretamente as demais variáveis. Maiores valores de massa seca de plantas foram observados quando o nitrogênio foi aplicado em dose total na semeadura. O teor de nitrogênio foliar não apresentou diferença entre os tratamentos devido à fontes ou épocas de aplicação de N, porém em 2006 os tratamentos que receberam N foram superiores à testemunha. O número de vagens planta-1 e de grãos planta-1 não foram influenciados...(Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Nitrogen is the nutrient uptaken in larger amount in common bean, and the management of the nitrogen fertilization can interfere in common bean crop yield. The work was developed at experimental station of University of São Paulo State - Ilha Solteira - Campus, located in Selvíria, State of Mato Grosso do Sul - Brazil, on a dystrophic clayey Haplic Acrustox cultivated in the previous five years with corn in the summer and common bean in the winter. The objective was to study the effect of sources and time of N application in common bean growth cropped under no-tillage, irrigated by sprinkle. Pérola cultivar was used and the experimental design was a randomized complete blocks, with 16 treatments in a factorial scheme 2 x 8: two sources of nitrogen (urea and ammonium sulfonitrate - fertilizer with inhibitor of nitrification), applied in seven times (30 days before sowing, 15 days before sowing, at sowing, 15 days after sowing, 30 days after sowing, 1/3 at sowing + 2/3 15 days after sowing, 1/3 at sowing + 2/3 30 days after sowing) and the control (without N) in two cropping years (2006 and 2007). The obtained results only revealed influence of sources in the stand at the second cropping year and the medium stand obtained in each cropping year might have influenced direct or indirectly the other results. Higher values of plant dry mass were obtained when the nitrogen was applied in total dosage at sowing. The nitrogen content on the leaves did not present difference among treatments; however, in 2006 the treatments that received N were superior to the control. The number of pods per plant and seeds per plant were not influenced by treatments. The number of seeds per pod and the mass of 100 grains were influenced by times of nitrogen application in 2006; however, with apparent relation to other production components results. The sources and nitrogen time application did not influence the grain yield of winter common bean in no till system. / Mestre

Embrapa - Empresa Brasileira de Pesquisa Agropecuária / O Brasil é o maior produtor mundial de feijão-comum (Phaseolus vulgaris L.) e o grão é produzido em todo território nacional. Em seu cultivo predominam sistemas de produção com baixo índice de adoção de tecnologia e uso de insumos, porém existe uma parcela de produtores tecnificados. Com o lançamento da cultivar de feijão-comum superprecoce BRS FC104 estudos de manejo específicos para ela fizeram-se necessários. O objetivo deste estudo foi de determinar a melhor densidade de plantas para a cultivar; a responsividade da cultivar frente a inoculação das sementes com as estirpes SEMIA 4077, SEMIA 4080 e SEMIA 4088 de Rhizobium tropici; e a melhor época e dose de nitrogênio que deve ser aplicada em cobertura para expressão máxima da produtividade de grãos, dos demais componentes de produtividade, da espessura dos grãos e do teor de proteínas dos grãos nos cultivos de 1ª e 3ª safras. O incremento do número de plantas por hectare aumentou a produtividade de grãos, seja com ou sem a inoculação. A inoculação das sementes com as estirpes SEMIA 4077, SEMIA 4080 e SEMIA 4088 de Rhizobium tropici não apresentou resultados satisfatórios para as variáveis estudadas. A aplicação de nitrogênio em cobertura é uma prática fundamental para se obter ganhos em produtividade de grãos na 3ª safra. A aplicação de nitrogênio na germinação das sementes reduziu a população final de plantas. / Brazil is the world’s largest producer of common bean (Phaseolus vulgaris L.) and grain is produced throughout the country. In its cultivation, systems of production with low index of technology use and inputs use predominate, but there is still a portion technified producers. With the launching of super early common bean cultivation BRS FC104 management studies specific for it are needed. The objective of this study is to determine the best density of plants for a cultivation; a responsiveness of the cultivation to seed inoculation with SEMIA 4077, SEMIA 4080 and SEMIA 4088 of Rhizobium tropici strains; and the best time and nitrogen dose that should be applied in coverage for maximum expression of grain yield, other productivity components, grain thickness and grain protein content in 1st and 3rd crop crops. The increase in number of plants per hectare increased grain yield, either with or no inoculation. Seed inoculation with the strains SEMIA 4077, SEMIA 4080 and SEMIA 4088 of Rhizobium tropici did not present satisfactory results for the studied variables. The application of nitrogen under cover is a fundamental practice to obtain gains in grain yield in the 3rd crop. The application of nitrogen in the germination of the seeds reduced the final population of plants. / Dissertação (Mestrado)

This diploma thesis is focused on the creation of model tasks (by means of an experiment) for the study of biology at secondary schools and concerns the effects of temperature on plant growth. The selected tasks utilize the new didactic approach by means of the so called inquiry based education. This method of teaching contributes to the development of individual deduction reasoning and encourages the students to actively think about the subject matter. The thesis provides the necessary theoretical background for the topic together with the description of methods and material needed for the conducting the experiments. Furthermore, the thesis describes the necessary instructions and procedures to conduct the experiment and its use in the secondary school teaching practice and presents the requisite methodical guidelines and worksheets for them. Simultaneously, the thesis verifies the didactic applicability of the experiment and the worked out materials through a questionnaire survey and monitored application of the model tasks at secondary schools. The main result of this diploma thesis is a modern, functional and verified method of teaching for high school about the effects of temperature on plant growth in accordance with modern approaches to education, which include own experiments, their...

Isoflavone synthase (IFS; CYP93C) plays a key role in the biosynthesis of the plant secondary metabolites, isoflavonoids. These phenolic compounds, which are well-known for their multiple biological effects, are produced mostly in leguminous plants (family Fabaceae). However, at least 225 of them have also been described in 59 other families, without any knowledge of orthologues to hitherto known IFS genes from legumes (with the single exception of sugar beet - Beta vulgaris, from the family Chenopodiaceae). In view of these facts, this masters thesis has focused on two main objectives: (1) to identify isoflavone synthase genes in selected leguminous and non-leguminous plants exploiting the PCR strategy with degenerate and non-degenerate primers, and (2) to find a system for the verification of the correct function of these genes. Our methodology for the identification of IFS orthologues was successfully demonstrated in the case of two examined legumes - Phaseolus vulgaris L. and Pachyrhizus tuberosus (Lam.) Spreng, in the genomic DNA of which the complete IFS sequences have been newly identified. To design a procedure for ascertaining the correct function of these genes and others once they have been completely described, a pilot study with IFS from Pisum sativum L. (CYP93C18; GenBank number...