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Genetic basis for the virulence of enterohemorrhagic Escherichia coli strain TW14359Morgan, Jason Kyle 02 May 2014 (has links)
Enterohemorrhagic Escherichia coli (EHEC) is a virulent pathotype of E. coli that is associated with major outbreaks of hemorrhagic colitis and the life-threatening kidney disease hemolytic uremic syndrome. For successful host colonization and attachment to the intestinal mucosa, EHEC requires the locus of enterocyte effacement (LEE) pathogenicity island, which encodes a type III secretion system (TTSS) responsible for secreting and translocating effector proteins into host colonocytes. Regulation of the LEE is primarily directed through the first operon, LEE1, encoding the locus encoded regulator (Ler), and occurs through the direct and indirect action of several regulators. The 2006 U.S. spinach outbreak of E. coli O157:H7, characterized by unusually severe disease, has been attributed to a strain (TW14359) with enhanced pathogenic potential including elevated virulence gene expression, robust adherence, and the presence of novel virulence factors.
Aim 1 of this dissertation proposes a mechanism for the unique virulence expression and adherence phenotype of this strain, and further expands the role for regulator RcsB in control of the E. coli locus of enterocyte effacement (LEE) pathogenicity island. Proteomic analysis of TW14359 revealed a virulence proteome consistent with previous transcriptome studies that included elevated levels of the LEE regulatory protein Ler and type III secretion system (T3SS) proteins, secreted T3SS effectors, and Shiga toxin 2. Basal levels of the LEE activator and Rcs phosphorelay response regulator, RcsB, were increased in strain TW14359 relative to O157:H7 strain Sakai. Deletion of rcsB eliminated inherent differences between these strains in ler expression, and in T3SS-dependent adherence. A reciprocating regulatory pathway involving RcsB and LEE-encoded activator GrlA was identified and predicted to coordinate LEE activation with repression of the flhDC flagellar regulator and motility. Overexpression of grlA was shown to increase RcsB levels, but did not alter expression from promoters driving rcsB transcription. Expression of rcsDB and RcsB was determined to increase in response to physiologic levels of bicarbonate, and bicarbonate-dependent stimulation of the LEE was shown to be dependent on an intact Rcs system and ler activator grvA. The results of this aim significantly broaden the role for RcsB in EHEC virulence regulation.
The bicarbonate ion (HCO3-) has been shown to stimulate LEE gene transcription through the LEE1 promoter, and is predicted to serve as a physiologic signal for EHEC colonization. Results from the previous aim demonstrated that bicarbonate induction of the LEE is mediated through the Rcs phosphorelay, and is dependent upon an intact global regulator of virulence grvA gene. However, the direct mechanism through which RcsB-GrvA regulates ler, and the contribution of GrvA to the virulence of EHEC is unknown. In Aim 2, the RcsB-GrvA regulon of EHEC was determined by RNA sequencing, and the contributions of each to virulence and stress fitness was explored. A significant increase in transcription of the gad genes for extreme acid resistance was observed for both EHEC strains TW14359grvA and TW14359rcsBgrvA compared to TW14359, and corresponded with a significant increase in acid survival for TW14359grvA during exponential growth. Therefore, a model by which RcsB-GrvA coordinate LEE expression with acid resistance through GadE was proposed. Finally, the temporal regulation of both rcsDB and grvAB operons in response to bicarbonate was defined using single copy luxE chromosomal reporter fusions. Taken together, these results demonstrate the role of RcsB and GrvA to EHEC virulence, and reveal a novel role for GrvA in of extreme acid resistance and LEE gene expression and in EHEC.
Finally, production of the ECP pilus has been demonstrated in enterohemorrhagic Escherichia coli O157:H7 (EHEC), and has been shown to be required for efficient adherence to epithelial cells during colonization. The first gene of the ecpRABCDE operon encodes a transcriptional regulator (EcpR) that positively regulates its own transcription, and promotes transcription and production of the downstream gene, ecpA, encoding the major ECP subunit EcpA. However, the distance between the ecpR and ecpA genes suggests the presence of regulatory elements that control ecpA directly. Therefore, it was hypothesized that an additional promoter was able to direct transcription of ecpA, independent of the promoter upstream of ecpR. To test this, promoter-lacZ transcriptional reporter fusions were created using the regions upstream of ecpR and ecpA to test for promoter activity, coupled with western blot analysis to detect EcpA in both wild-type and ecpR promoter mutant strains. In Aim 3, we showed that an additional promotable element, downstream of the EHEC O157:H7 strain TW14359 ecpR translational start site, is capable of driving transcription of ecpA, and that its activity is independent of an intact ecpR promoter. In addition, site-directed mutagenesis was used to characterize a TW14359 specific single nucleotide polymorphism within the predicted ecpA promoter region. Overproduction of EcpR was observed to increase cytosolic RcsB and Tir, indicating that ecp production is able to stimulate the LEE, and that the ecpA promoter polymorphism may contribute to intrinsically increased rcsB transcription in TW14359. Taken together, the results, and those obtained in Aims 1 and 2, expand the model for regulation of the ecp operon in EHEC O157:H7 strain TW14359, and broaden the model for EcpR and RcsB in the coordinate regulation of E. coli common pilus and type III secretion.
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VanT, a central regulator of quorum sensing signalling in Vibrio anguillarumCroxatto, Antony January 2006 (has links)
Many bacteria produce signal molecules that serve in a cell-to-cell communication system termed quorum sensing. This signalling system allows a bacterial population to co-ordinately regulate functions according to their cell number in a defined environment. As bacterial growth progresses towards the stationary phase, signalling molecules accumulate in the growth medium and, above a certain threshold level, regulate the expression of genes involved in diverse functions. Most of the functions monitored by quorum sensing are most beneficial when they are performed as a population than by single cells, such as virulence factor production, biofilm formation, conjugation and bioluminescence. Vibrio anguillarum is a bacterial pathogen that causes terminal hemorrhagic septicaemia in marine fish. V. anguillarum possesses multiple quorum sensing circuits similar to the LuxI/LuxR and the V. harveyi-type systems. In this study, a characterisation of the quorum sensing-regulated transcriptional activator VanT was made. VanT belongs to the V. harveyi LuxR family of transcriptional regulators, which play a central role in quorum sensing signalling in Vibrio species. VanT was shown to regulate serine, metalloprotease, pigment, exopolysaccharide (EPS) and biofilm production. VanT repressed an EPS locus that plays a critical role in bacterial colonization of the fish integument and virulence. The V. harveyi-like quorum sensing systems were shown to limit rather than induce vanT expression throughout growth in V. anguillarum. In contrast to homologous proteins in other Vibrio spp., the quorum sensing phosphorelay protein VanU and the response regulator VanO had antagonistic roles in the regulation of vanT expression. Unlike other members of the luxR family, vanT was expressed at low cell density and no significant induction due to quorum sensing regulation was seen. Interestingly, VanT expression was induced by the alternative sigma factor RpoS as the cells entered stationary phase. RpoS was shown to regulate VanT expression post-transcriptionally by promoting vanT mRNA stability. VanT and RpoS were important for bacterial survival under stress conditions, indicating that VanT is likely an essential factor of V. anguillarum stress response.
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Identification et caractérisation de facteurs de transcription appartenant à la famille des régulateurs de réponse de type B, impliqués dans la réponse à la sécheresse chez le peuplier / Identification and characterization of B-type response regulators transcription factors involved in the poplar osmosensing pathwayDjeghdir, Inès 15 December 2016 (has links)
Les plantes sont de plus en plus confrontées à une diminution de la disponibilité en eau du sol, constituant une contrainte hydrique et osmotique impactant leur survie. La tolérance des plantes face à cette contrainte sera conditionnée par la perception de celle-ci. Un des mécanismes de signalisation de cette contrainte est appelé MultiStep Phosphorelay (MSP) et est composé de 3 partenaires : un récepteur Histidine-aspartate Kinase (HK), des protéines Histidine Phosphotransfert (HPt) et des Régulateurs de Réponse (RR), dont les facteurs de transcription RR-B. Chez Arabidopsis, un MSP constitué d’AHK1, AHP2 et ARR18 a été identifié dans le cadre de la contrainte osmotique. Pour le peuplier, HK1a et b, gènes paralogues et homologues à AHK1, ainsi que 10 et 9 gènes codant respectivement des HPt et des RR-B ont été isolés. La fonction d’osmosenseur d’HK1a a été avancée, et une voie de signalisation de la contrainte osmotique chez le peuplier constituée de ce récepteur, 3 HPt et 6 RR-B a été proposée. L’objectif de la thèse visait à déterminer et caractériser des facteurs de transcription RR-B liés à la contrainte osmotique de façon spécifique. Les résultats phares de cette thèse sont la mise en évidence de la fonction de facteur de transcription de deux RR-B, RR13 et RR19, via l’étude de leur capacité à dimériser et à transactiver ou non des gènes de réponses à la contrainte osmotique. Le RR13 semblerait spécifique de la voie cytokinines et le RR19 de la voie osmosensing. Ce travail étaye fortement l’implication du RR19 dans le MSP dédié à cette contrainte. De nombreuses études ont par ailleurs été initiées durant ce travail de thèse et pourront faciliter la caractérisation du MSP étudié. / Plants are increasingly faced with a decrease in soil’s water availability, leading to a hydric and osmotic stress and impacting on their survival. Plant tolerance to this stress will be dependent on its perception. One of the signaling mechanisms related to this stress is called MultiStep Phosphorelay (MSP) and is composed by 3 partners: a histidine-aspartate receptor kinase (HK), histidine phosphotransfer proteins (HPt) and response regulators (RR), including the B-type RR transcription factors. In Arabidopsis, an MSP with AHK1, AHP2 and ARR18 has been identified for osmotic stress signaling. For poplar, HK1a and b, paralogous genes and homologous with AHK1, 10 HPt and 9 B-type RR genes have been isolated respectively. The osmosensor function of HK1a was proposed, and an osmosensing signaling pathway composed by HK1a, 3 HPt proteins, and 6 B-type RR has been suggested. The purpose of this work was focused on the identification and characterization of B-type RR transcription factors specifically linked to osmotic stress in poplar. The main results of this work are the highlight of the transcription factor function of two B-type RR, RR13 and RR19, through the study of their ability to dimerize and transactivate or not osmotic stress-responsive genes. The RR13 seems to be specific for cytokinins signaling pathway, whereas the RR19 seems to be specific for the osmosensing one. This work strongly supports the involvement of RR19 in the osmosensing MSP. Many studies have also been initiated during this work and will facilitate the characterization of the studied MSP.
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Rôle des systèmes à deux composants dans l’adaptation de la bactérie phytostimulatrice Azospirillum à la rhizosphère / Role of two component systems in the adaptation of the phytostimulatory bacterium Azospirillum to the rhizosphereBorland, Stéphanie 02 April 2015 (has links)
Les systèmes à deux composants jouent un rôle prépondérant dans l'adaptation des bactéries à leur environnement. L'objectif de ce travail de thèse était d'identifier et de caractériser des systèmes à deux composants chez la bactérie phytostimulatrice Azospirillum nécessaires à l'adaptation à la rhizosphère de sa plante-hôte. L'analyse de la distribution génomique des gènes appartenant à la famille des systèmes à deux composants dans les génomes d'Azospirillum disponibles a révélé l'existence d'un grand nombre de gènes codant des hisitidine kinases hybrides, et une analyse plus approfondie a montré une organisation multidomaines complexe de cette famille de protéines. Afin de comprendre leur rôle chez Azospirillum, nous avons, dans un premier temps, sélectionné et inactivé quatre gènes codant des histidine kinases hybrides présentant une architecture multidomaines complexe. A l'aide d'une approche multidisciplinaire combinant génétique, biochimie et phylogénie, nous avons mis en évidence pour la première fois chez Azospirillum, un système atypique à trois-composants nommé PreSKR contrôlant un grand nombre de processus impliqués dans la survie et la colonisation de la rhizosphère, qui agirait en modulant le taux intracellulaire de c-di-GMP. Dans un second temps, nous nous sommes focalisés sur une histidine kinase hybride exprimée au contact de la plante hôte ; cette protéine, appelée RsiK, s'avère être impliquée dans la perception de surfaces et la régulation de la formation de biofilms. L'analyse du régulon par RNA-seq a révèlé que 78 gènes étaient contrôlés par ce système. La prévalence de la famille des histidine kinases hybrides chez Azospirillum couplée à l'approche fonctionnelle réalisée sur deux d'entre elle souligne l'importance des phosphorelais encore largement méconnus chez les bactéries rhizosphériques / Bacterial two-component systems play an important role in the ability of bacteria to adapt to various environments. The aim of this thesis was to identify and characterize two-component systems involved in the adaptation of the phytostimulatory bacteria Azospirillum to its host plant. Analysis of the genomic distribution of genes encoding two-component systems across Azospirillum available genomes revealed the existence of a high number of genes encoding hybrid histidine kinases, and further analyses highlighted a complex multi-domain organization of this family of proteins. In order to understand their role in Azospirillum, as a first step we selected and inactivated four genes encoding complex hybrid histidines kinases. Using a multidisciplinary approach which combines genetics, biochemistry and phylogeny, we brought to light for the first time in Azospirillum, an atypical three-component system named PreSKR which controls a wide variety of processes involved in survival and rhizosphere colonization likely by modulating c-di-GMP levels. As a second step, we focused on a gene encoding a hybrid histidine kinase named RsiK which is induced in contact with its host plant. RsiK is involved in surface sensing and biofilm formation regulation. Transcriptomic analysis of rsiK regulon by RNA-seq showed that 78 genes were under the control of this system. The prevalence of genes encoding hybrid histidine kinase family in Azospirillum, coupled with the functional characterization of two of them, highlight the importance of phosphorelays, still largely unrecognized in rhizospheric bacteria
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Caractérisation d'un phosphorelais multiple de type histidine-aspartate dans la transduction du signal de la contrainte osmotique chez le peuplier : mécanismes de régulation du fonctionnement d'un régulateur de réponse de type-B à l'échelle moléculaire / Characterization oft he multistep His-to-Asp phosphorelay system in the osmosensing pathway in poplar : regulatory mechanisms at the molecular scale of a B-type response regulator functionBertheau, Lucie 19 December 2013 (has links)
Les relais de phosphorylation de type histidine/aspartate constituent des voies de signalisation impliquées dans la perception et la transduction des signaux jusqu’à la mise en place de réponses spécifiques. Ils mettent en jeu un récepteur ou Histidine aspartate Kinase (HK), des protéines navettes en charge de la transmission du phosphate (HPt) et des Régulateurs de Réponse (RR). L’implication d’un tel système dans la transduction du signal de la contrainte osmotique est avérée chez la levure et fortement suspectée chez Arabidopsis. Ce travail de thèse visait d’une part à caractériser l’implication de cette voie de transduction de la contrainte osmotique chez le peuplier, avec l’identification de partenaires HPt et RR en aval du récepteur HK1 et d’autre part à caractériser le mode de fonctionnement d’un RR de type-B. HK1, un osmosenseur membranaire détecterait le signal et le transmettrait à trois HPt préférentielles. De plus, un partenariat d’interaction se dégagerait entre ces trois HPt et certains RR-B. La régulation transcriptionnelle observée lors d’une contrainte osmotique pour deux des représentants des RR-B témoigne d’une possible implication de ces RR dans cette voie. Ces protéines sont des facteurs de transcription dont la fonction a été confirmée in planta pour l’un d’entre eux. La dimérisation du domaine receveur du RR et son interaction avec le domaine de fixation à l’ADN ou domaine GARP apparaissent comme des points de contrôle clés dans la régulation de l’activité effectrice des RR-B. De plus, la capacité d’un RR-B à se fixer sur ses motifs de reconnaissance (boîtes AGAT) a pu être vérifiée in vitro et la présence de ces séquences a d’ailleurs été retrouvée dans des gènes régulés par la contrainte osmotique. Ce travail prospectif ouvre des perspectives concernant l’implication des RR-B dans la voie de transduction du signal de la contrainte osmotique, et propose notamment des mécanismes fins pour l’élaboration d’une réponse hautement spécifique. / Multistep His-to-Asp phosphorelay systems are signaling pathways devoted to signal perception and transduction for establishment of specific responses. These systems are composed of three successive partners: Histidine-aspartate Kinases (HKs), Histidine-containing Phosphotransfer proteins (HPts), and Response Regulators (RRs). One of the best characterized corresponding systems is the osmo-responsive pathway in yeast. Such systems are also suspected in Arabidopsis. This work aimed to characterize the involvement of an osmosensing pathway in Populus by identifying HPt and RR elements downstream of HK1 and to reveal the underlying mechanisms for the activity of a RR-B. HK1, membrane osmosensor, is expected to be responsible for signal detection and propagation by triggering the activation of three preferential HPt. Furthermore, an interacting partnership between those HPts and particular B-type RRs was observed. Two of them appear to be regulated by an osmotic stress, suggesting their possible involvement in this pathway. The B-type RR members, the final output elements of the pathway, act as transcription factors, as shown for at least for one of them in planta. Taken together, the dimerization of the RR receiver domain and its interaction with its DNA binding domain (GARP), are likely key checkpoints in the regulation of RR-B activity. Besides, the ability of one RR-B to bind its cognate specific DNA sequences (AGAT boxes) was confirmed in vitro and those were found in promoters of osmotic response genes. This work opens up prospects for the involvement of RR-B in the osmotic stress signaling pathway and suggests mechanisms tuning induction of specific responses.
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