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The aim of this thesis is to understand the current implementation, how different hardware and output frequency affects the hydraulic actuators in the current platform and Then an improve the controller should be presented. This needs to be both faster then the current controller and should not use more CPU recurses then necessary. With the understanding of current controller, three new regulators where implemented and tested. One uses a PI regulator and the other two uses an adaptive algorithm to generate the control signal. All where faster than the current one and the PI-implementation uses the lowest amount of CPU recurses, on the other hand this needs to be calibrated for the different hardware and output frequency’s. ThetwoadaptivecontrollersrequiresahigheramountofCPUrecurses, instead it requires less calibration to work.

There is a need for quantitative CT data in radiation therapy. Currently there are only few algorithms that address this issue, for instance the commercial DirectDensity algorithm. In scientific literature, an example of such an algorithm is DIRA. DIRA is an iterative model-based reconstruction method for dual-energy CT whose goal is to determine the material composition of the patient from accurate linear attenuation coefficients. It has been implemented in a two dimensional geometry, i.e., it could process axial scans only.  There was a need to extend DIRA so that it could process projection data generated in helical scanning geometries. The newly developed algorithm (DIRA-3D) implemented (i) polyenergetic semi-parallel projection generation, (ii) mono-energetic parallel projection generation and (iii) the PI-method for image reconstruction. The computation experiments showed that the accuracies of the resulting LAC and mass fractions were comparable to the ones of the original DIRA. The results converged after 10 iterations.

Listeria monocytogenes is a Gram-positive facultative intracellular bacterium with a wide ecological niche and causes a number of diseases in human and animals. It invades mammalian host cells and escapes from the vacuoles prior to replication in the host cell cytoplasm and infecting adjacent cells via actin-based mobility. Phosphoinositide (PIP) metabolism is essential to mammalian cells in signal transduction, actin remodelling, endosome dynamics and membrane trafficking. Modulation of host PIP metabolism by bacteria PIP phosphatases is important for pathogenicity and virulence of many human pathogens. In this study the function of two L. monocytogenes tyrosine and inositol phosphatases LipA and LipB were studied in vitro. The lipA and lipB deletion mutants generated in EGDe and InlA strains were not affected in invasion but were attenuated in intracellular growth in Caco-2 and Hela M cell lines but not in mouse macrophages. Deletion of lipA or lipB did not affect the actin polymerisation but caused reduced plaque number in the plaque assay. The turnover of five PIPs in Hela M cells during L. monocytogenes infection were studied by expression of fluorescent protein tagged domains that specifically recognizes individual PIPs. L. monocytognenes did not affect the metabolism of PI4P, PI(4,5)P2, PI(3,4,5)P3 but co-localised with PI3P at 1.5 hr post-infection and with PI(3,4)P2 at 6 hr to 24 hr post-infection. The PI(3,4)P2 effector protein lamellipodin was discovered to be recruited to actin-associated L. monocytogenes at 4 hr to 24 hr post-infection in Hela M cells. This discovery leads to the hypothesis of a novel mechanism of lamellipodin-dependant cell-to-cell spread. The lipA mutant was found to be attenuated in PI(3,4)P2 recruitment and therefore hypothesized to participate in the proposed lamellipodin pathway by converting PI(3,5)P2 into PI5P, leading to the activation of PI3K and subsequent production of PI(3,4)P2. LipB showed partial localisation at the Golgi complex when over-expressed in Hela M cells, and it was assumed to act mainly as a protein-tyrosine phosphatase. In summary, this study provides some evidence on L. monocytogenes modulating host PIP metabolism by the production of inositol phosphatases. It gives us a better understanding on the intracellular growth of this pathogenic bacterium, and on the interaction between host and parasite.

The current most common methods for measuring a blood vessels flow with Doppler technique requires a cable between the patient and measuring instrument. In today’s technology and the progress made in microelectronics have made it possible to manufacture ultrasonic transmitters and receivers, control electronics and antennas small enough for them to be integrated in a probe attached to the blood vessel. In order to read the flow of blood used NFC to securely send the information wireless to a smartphone or a tablet. This ensures that the cable between the patient and the measuring instrument would not be needed and the patient possibility to move would increase. So this thesis was to continue on a prototype using a Raspberry Pi and other medical equipment to approach toward the ultimate objective. So with help of filtering and amplification the target was to reduce noise and amplify the signal so that the correct data was send to the recipient’s smartphone or tablet. / De nuvarande vanligaste metoderna för att mäta upp ett blodkärls blodflöde med Doppler-teknik kräver en kabel mellan patient och mätinstrument. I dagens teknik och de framsteg som har gjorts inom mikroelektroniken har gjort det möjligt att tillverka ultraljudssändare och mottagare, styrelektronik och antenner i tillräckligt liten storlek för att de ska kunna integreras i en prob som fästs vid blodkärlet. För att kunna läsa av blodflödet används NFC för att säkert kunna skicka över information trådlöst till en smartphone eller en surfplatta. Detta medför att kabeln mellan patienten och mätinstrumentet inte skulle behövas och patientens möjlighet att röra sig skulle öka. Så det här examensarbetet gick ut på att fortsätta på en prototyp med hjälp av en Raspberry Pi och annan medicinsk utrustning för att närma sig det slutgiltiga målet. Så med hjälp av filtrering och förstärkning är målet att minska störningar och förstärka signalen så att rätt data skickas till mottagarens smartphone eller surfplatta.

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The goal of this diploma thesis is to verify the practical applicability of the Raspberry Pi platform in control applications. The work consists of choosing a suitable operating system and implementing a discrete PID algorithm. An important part of the work was the Linux OS modification and compilation. Measurement has demonstrated the usability of the platform in control applications. The result of this work is a discreet PID controller implemented as a Linux kernel module. The solution also includes a web interface as a human-machine interface.

This thesis solves the development of Power Line Monitor. The thesis also describes the specific implementation of hardware, software and results.

The essence of this work is to summarize the knowledge about 3D printing, especially with FDM technologies. Create an overview of used FDM printing materials, identify their advantages and disadvantages, technical parameters and usability. Another purpose of the thesis is to examine the print quality and its problems and specify types of surface finishes for selected materials. The content also includes remote control and monitoring of the 3D print process using the Raspberry Pi microcomputer and the print server named Octoprint.

This thesis deals with design and implementation of wireless system for automated control and monitoring of central heating. Some of the existing solutions and their characteristics are briefly introduced at first. The attention in this thesis is subsequently given to the analysis of wireless technologies and power supply methods suitable for home automation. With regard to ascertained findings, the design and subsequent implementation of system modules using Raspberry Pi, the ARM Cortex-M0+ microcontroller and the ZigBee communication modules were performed. The firmware for created modules was designed and implemented. The designed system can be controlled and monitored using a mobile application for the Android platform, whose design is also described in this thesis.