The purpose of this study was to study the rhymes in P'i Pa Chi, a drama from South China in the Yuan Dynasty. To achieve this purpose the rhyme words of P'i Pa Chi were gathered and divided into rhyming categories. These were compared with earlier and contemporary phonological works such as rhyme tables, rhyme dictionaries etc. and some particular problems were studied. P'i Pa Chi's rhyme categories, of course, cannot provide every single detail of real speech, but they do indicate certain differences as compared with contemporary Northern Drama which are important clues to an understanding of the phonological phenomena in Southern China in the Yuan Dynasty. / Arts, Faculty of / Asian Studies, Department of / Graduate

This thesis summarizes our project in building and setting up a Beowulf cluster. The idea of the project was brought forward by the company CGI in Karlstad, Sweden. CGI’s wish is that the project will serve as a starting point for future research and development of a larger Beowulf cluster. The future work can be made by both employees at CGI and student exam projects from universities. The projects main purpose was to construct a cluster by using several credit card sized single board computers, in our case the Raspberry Pi 3. The process of installing, compiling and con- figuring software for the cluster is explained. The MPICH and TensorFlow software platforms are reviewed. A performance evaluation of the cluster with TensorFlow is given. A single Raspberry Pi 3 can perform neural network training at a rate of seven times slower than an Intel system (i5-5250U at 2.7 GHz and 8 GB RAM at 1600 MHz). The performance degraded significantly when the entire cluster was training. The precise cause of the performance degradation was not found, but is ruled out to be in software, either a programming error or a bug in TensorFlow.

No description available.

Thesis advisor: Shih-Yuan Liu / Described herein are two projects on the application and effects of BN/CC isosterism on indole-containing compounds. In the first chapter, the synthetic route to an unnatural boron and nitrogen-containing analogue of tryptophan (BN-tryptophan) via late-stage functionalization of BN-indole is disclosed and its spectroscopic properties are reported with respect to the natural amino acid, tryptophan. The incorporation of BN-tryptophan into proteins expressed in E. coli using selective pressure incorporation, a residue specific method of unnatural amino acid incorporation, is then reported and its reactivity and fluorescence in the proteins characterized. In the second chapter, the synthesis of a BN-indole-containing aromatic scaffold is reported and the cation-π binding ability characterized by nuclear magnetic resonance (NMR) monitored titrations is disclosed. The resulting chemical shifts were analyzed using a non-linear curve fitting procedure and the extracted association constants (Ka’s) compared with the natural indole scaffold. Computations were also performed to support the titration results. / Thesis (PhD) — Boston College, 2020. / Submitted to: Boston College. Graduate School of Arts and Sciences. / Discipline: Chemistry.

The focus of this dissertation is to understand the physicochemical factors that affect the spatiotemporal control of phosphoinositide signaling events. Despite their low abundance in cellular membranes ( ~ 1% of total lipids) phosphoinositides are assuming major roles in the spatiotemporal regulation of cellular signaling, therefore making this group of lipids an attractive area of study, especially for identifying drug targets. The main phosphoinositide studied in this dissertation is phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], which regulates various intracellular signaling pathways, notably the PI3K/AKT pathway. The PI3K/AKT pathway plays a critical role in regulating diverse cellular functions including metabolism, growth, proliferation, and survival. Thus, dysregulation of the PI3K/AKT pathway is implicated in a number of human diseases including cancer, diabetes, cardiovascular disease and neurological diseases. PI(4,5)P2 regulates phosphoinositide signaling in the PI3K/AKT pathway through interaction of its highly anionic headgroup with polybasic proteins. The highly specific manner that allows hundreds of structurally diverse proteins to interact with lipid species found in such low supply may require the local formation of PI(4,5)P2 clusters (domains). Although a significant amount of evidence has accumulated over the past decade that supports the notion of PI(4,5)P2-rich clusters, our understanding regarding the structural determinants required for cluster formation remains limited. Studies have shown that PI(4,5)P2 clustering is induced by cellular cations interacting with PI(4,5)P2 via electrostatic interactions, suggesting that non-clustering/clustering transitions are particularly sensitive to ionic conditions. However, why some ions are more effectively cluster PI(4,5)P2 than others remains to be understood. For our first research aim, we investigated the effects of divalent (Ca2+) and monovalent cations (Na+, K+ ) on PI(4,5)P2 clustering to understand the ionic environment required for electrostatic PI(4,5)P2 cluster formation. We used monolayers at the air/water interface (Langmuir films) to monitor PI(4,5)P2 molecular packing in the presence of each cation. Our results indicated that Ca2+ individually and Ca2+ along with K+ had a greater effects on PI(4,5)P2 cluster formation than Na+ and K+, individually and combined. We hypothesize that the cations shield the negatively charged headgroups, allowing adjacent PI(4,5)P2 molecules to interact via H- bonding networks. The analysis of the electrostatic environment required for stable PI(4,5)P2 clustering will help us understand important aspects of PI(4,5)P2 mediated signaling events, such as the temporal control of protein binding to PI(4,5)P2 clusters to enhance their function. Another important spatiotemporal modulator that affects the local concentration of PI(4,5)P2 clusters is cholesterol, a steroid present in large quantities (30-40 mole%) in the plasma membrane. Cholesterol has been shown to induce the formation of liquid-ordered domains when interacting with an otherwise gel phase forming lipid, however, the interaction of cholesterol with an inner leaflet lipid species that favors more of a disordered environment to form clusters is poorly understood. We hypothesize that cations along with cholesterol work synergistically to induce PI(4,5)P2 clustering. Thus, our second research aim was to investigate the role of cholesterol on PI(4,5)P2 clustering by monitoring the molecular packing of PI(4,5)P2 in the presence of both cholesterol and cations. This aim was investigated similarly to the first aim with Langmuir trough monolayer film experiments. Our results showed that cholesterol in the presence of Ca2+ had an additive effect leading to the strongest condensation of the monolayer (increase in PI(4,5)P2 packing). Our hypothesis is that Ca2+ significantly reduces the negative electron density of the phosphate groups, allowing the cholesterol hydroxyl group to interact with PI(4,5)P2 headgroup through hydrogen-bond formation. To confirm our hypothesis, we collaborated with a computational group at the NIH that performed all-atom molecular dynamics (MD) simulations that closely agreed with our experimental data. Thus we were able to determine that the cholesterol hydroxyl group directly interacts via hydrogen-bonding with the phosphodiester group as well as the PI(4,5)P2 hydroxyl groups in the 2- and 6-position. The insight into the structural positioning of cholesterol moving closer to the PI(4,5)P2 headgroup region suggests this unique interaction is important for PI(4,5)P2 cluster formation. Other anionic lipid species are suspected to interact with PI(4,5)P2 and strengthen PI(4,5)P2 clustering. We were particularly interested in the interaction of PI(4,5)P2 with phosphatidylinositol (PI) and phosphatidylserine (PS) because both are abundant in the plasma membrane, ~6-10% and ~10-20% respectively, and both electrostatically bind to peripheral proteins. Therefore, the third research aim analyzed the capacity of PI and PS to form stable clusters with PI(4,5)P2. We hypothesize that a mixed PI/PI(4,5)P2 or PS/PI(4,5)P2 domains are ideal for protein binding, since in combination PI or PS with PI(4,5)P2 would provide the necessary negative electrostatic environment, while PI(4,5)P2 would provide the high specificity and additional electrostatics for protein binding. Langmuir trough monolayer films were used to investigate the stabilization of PI/PI(4,5)P2 and PS/PI(4,5)P2 monolayers in the presence of Ca2+. Our results showed a condensation of the monolayer for both PI/PI(4,5)P2 and PS/PI(4,5)P2 with an increase in Ca2+concentrations, which suggests that Ca2+ shields the highly negatively charged phosphomonoester groups of PI(4,5)P2 allowing PI and PS to participate in PI(4,5)P2’s hydrogen-bond network. Interestingly, both PI and PS equally stabilized PI(4,5)P2 cluster formation, therefore it is highly likely that these lipids interact in vivo to form large stable electrostatic domains required for protein binding. The first three aims provided us with information about the physiological relevant environments required for PI(4,5)P2 cluster formation, while the last aim was geared towards understanding the temporal control of protein association with phosphoinositides in the plasma membrane. Specifically, we analyzed the plasma membrane association of PTEN-L, a translation variant protein of PTEN, that has the ability to exit and enter back into cells, unlike classical PTEN. The ability of PTEN-L to facilitate entry across the anionic and hydrophobic layers of the plasma membrane (in the case of direct transport of PTEN-L across the membrane) or into phospholipid transport vesicles (in the case of vesicular transport of PTEN-L across cells) is likely due to the addition of the 173 N-terminal amino acids, the alternative translated region (ATR-domain). Thus, our fourth research aim focused on the biophysical role of the ATR-domain to associate with inner leaflet plasma membrane lipids. Using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy to monitor secondary structural changes of the ATR-domain upon lipid binding, it was revealed that both PS and PI(4,5)P2 induced conformational change towards a slight increase in β-sheet content in an otherwise unstructured domain suggesting these lipids are required for ATR-domain interaction with the PM. Further studies revealed that the ATR-domain affects the integrity of PS lipid vesicles, further indicating the presence of PS is required to drive ATR-domain across the membrane. This aim provides information on ATR-domain lipid binding preferences aiding in our understanding of the biological and functional role of PTEN-L as a deliverable tumor suppressor protein. The overall goal of the research in this dissertation is to understand factors that fine-tune PI(4,5)P2 cluster formation in space and time. Our first three research aims were designed to understand the synergistic effects of spatiotemporal modulators (cations, cholesterol, and anionic lipids) on local concentration of PI(4,5)P2 clusters. Our results indicate that Ca2+, cholesterol, and the presence of anionic lipids PI and PS all induce stable domains, thus it is highly likely this is part of the biological environment required in vivo for cationic proteins to bind. The last aim, the association of the ATR-domain with phospholipids in the plasma membrane, provided evidence that PS is likely required to drive the ATR-domain across the plasma membrane. This dissertation unifies nearly two decades worth of research by shedding light on synergistic modulators of PI(4,5)P2 cluster formation (Figure 1). Thus, this work has potentially far reaching consequences for understanding temporal control of the spatially resolved protein activity.

The web information behaviors of users as they interacted with a metadata navigator, the Personal Information (PI) Agent, and reflected upon their interaction experiences were studied. The process included studying the complete iterative (repeated) cycle of information needs, information seeking, and information use of users interacting with an internet-based prototype metadata PI Agent tool. Detlor’s theory of web information behaviors of organizational users was utilized as a theoretical foundation for studying human-information interactions via the PI Agent tool. The qualitative research design allowed for the use of triangulation within the context of a one-group pretest-posttest design. Triangulation occurred in three phases: (a) observe, (b) collect, and (c) reflect. Observations were made as participants solved three problem situations. Participants’ computer log and print screen data were collected, and follow-up interviews were conducted once all posttest sessions ended to enable users to reflect on their experiences. The three triangulation phases ensured saturation of data and greater depth regarding the participants’ information behaviors. Content analysis occurred via exploratory pattern analysis using the posttest Problem Steps Recorder (PSR) log data and on the six interviewees’ follow-up interview data. Users engaged in iterative cycles of information needs, information seeking, and information use to resolve the presented problem situations. The participants utilized the PI Agent tool iteratively to eliminate their knowledge gaps regarding the presented problem situations. This study was the first to use PSR log data for capturing evidence of the iterative search process as defined by Detlor. The implications for best practices were inspired by participant feedback, and recommendations for further study are made.

The report aims to investigate how well a certain type of affordable embedded single board computer can hold up against today's more expensive computers in a computer system by doing various tests on a system with the specified requirements. The system has a Raspberry Pi as the single board computer which task is to control a camera based on coordinates obtained from a server as well as capture and stream a video signal on a network. The researches were conducted to check how much network traffic a single-chip computer sent in different video formats and how much CPU utilization was required. Studies were also made to ensure the accuracy of the camera control. The researches have been experimental, where several tests have been performed and analyzed. The results show that a sufficiently good accuracy can be obtained from the camera steering unit, in which two different servos have been investigated. When the video format MJPEG and H.264 are used, the single-chip computer is able to transmit a video signal up to 1280x720 at 15 fps. The system managed to download and perform calculations on an object from the server at 42.3 ms. When the entire system was up and running at the same time the Raspberry Pi didn’t manage to deliver a video signal and obtain the coordinates from the server fast enough. Depending on the video format the performance on the single-chip computer varied, but no setup managed to keep the system stable enough to reach the requirements. / Rapportens syfte är att undersöka hur väl en viss typ av billigare enkortsdator kan stå sig mot dagens dyrare datorer i ett datorsystem genom att göra olika undersökningar på ett system med uppsatta krav. Systemet har en Raspberry Pi som enkortsdator och har till uppgift att styra en kamera utifrån koordinater som fås från en server samt fånga och strömma en videosignal ut på ett nätverk. De undersökningar som gjordes var att kontrollera hur mycket nätverkstrafik som enkortsdatorn sände vid olika format på videosignalen samt hur mycket CPU- utnyttjande som krävdes. Undersökningar gjordes också för att säkerställa precisionen på kamerastyrningen. Alla undersökningar har varit experimentella, där flera olika tester har utförts och analyserats. Resultatet från undersökningarna visar att en tillräckligt god precision kan fås från kamerastyrningen, där två olika servon har undersökts. När videoformaten MJPEG och H.264 används kan enkortsdatorn klara av att sända ut en videosignal upp till 1280x720 med 15 bildrutor per sekund. I systemet som testerna utfördes på klarade enkortsdatorn av att hämta och utföra beräkningar på ett objekt från servern på 42,3 ms. När hela systemet var igång samtidigt klarade dock inte Raspberry Pi av att leverera en videosignal och hämta koordinater från servern tillräckligt snabbt. Beroende på vilket videoformat som användes presterade enkortsdatorn olika bra, men det var ingen inställning som stabilt klarade av att nå kraven.

Philosophiae Doctor - PhD / Growth of symbiotic legume hosts is P limited, because of the high energetic requirements associated with N2 fixation. Attempts to overcome P deficiency in soils where legumes are grown involve addition of P-based fertilisers. However, these are produced from fmite, non-renewable resources that could be exhausted in the next 50-80 years. For this and other prudent reasons, viable alternatives are sought that include producing genetically enhanced plants with better P use efficiency (PUE). There exist some inter- and intraspecific genetic variation for associated traits of PUE in various legumes and these will have to be exploited to realize the development of P efficient cultivars. With the advent of sophisticated molecular tools, good progress has been made to understand the molecular response of some common physiological and morphological functions observed under LP. The research aims here were to investigate the energy costs and the alternative metabolic routes associated with C and N metabolism under LP in legumes, which is very scant in literature. We also investigated the recovery responses of nodulated roots upon P alleviation. Consequently, improvement strategies to produce legume varieties for better adaptation in poor P soils are envisaged. We have demonstrated varying degrees of sensitivity between the amide and ureide legume systems being investigated under short-term LP. The species-specific responses were ascribed to differences related to the agro-climatic origins, nodule morphologies and the type of N containing export product of the different legume types. These different responses also underscore possible different regulatory mechanisms under LP. Lupins were probed further, because of its apparent tolerance to P deficiency. Lupin nodules had between 3 to 5-fold higher Pj concentrations compared with soybeans under LP and HP, respectively. The maintenance of Pj levels, as oppose to a decline in the total P pool, is discussed in relation to its role in maintaining N2 fixation in lupins. Under LP, an effective Pj recycling mechanism in nodules is proposed to occur via the induction of the PEPc- MDH-ME route. This route also enhanced the capacity of root nodules to procure high malate concentrations that are used to fuel bacteroid respiration and N2 fixation. Two distinctly different cMDH proteins, one corresponding to HP and another corresponding to LP, were identified. The high malate concentrations reported here are speculated to have arisen through LP-induced cMDH. Metabolically available Pj decline developed gradually as P deficiency progressed. This coincided with a 15% decline in the %Ndfa. Moreover, under prolonged P deficiency the disproportionate synthesis of organic acids, most notably malate, that occurred at the expense of amino acids was proposed to account for this decline. The recovery in response to alleviation from LP involved alterations in the allocation of respiratory costs to growth and nutrient acquisition. Under LP, smaller nodules were formed and nodule metabolism revolved around accentuating PUE. Thus, there is considerable potential for improvement of P efficiency in legumes through manipulation of root: shoot partitioning.

The purpose of this research is to investigate the structural efficiency of 8-node IsoTruss structures subject to uniaxial compression using numerical, dimensional, and optimization methods. The structures analyzed herein are based on graphite/epoxy specimens that were designed for light-weight space applications, and are approximately 10 ft. (3 m) long and 0.3 lb. (0.14 kg). The principal failure modes considered are material failure, global buckling, local buckling at the bay level, and longitudinal strut buckling. Studies were performed with the following objectives: to correlate finite element predictions with experimental and analytical methods; to derive analytical expressions to predict bay-level buckling; to characterize interrelations between design parameters and buckling behavior; to develop efficient optimization methods; and, to compare the structural efficiency of outer longitudinal configurations with inner longitudinal configurations. Finite element models were developed in ANSYS, validated with experimental data, and verified with traditional mechanics. Data produced from the finite element models were used to identify trends between non-dimensional Pi variables, derived with Buckingham's Pi Theorem. Analytical expressions were derived to predict bay-level buckling loads, and verified with dimensional analyses. Numerical and dimensional analyses were performed on IsoTruss structures with outer longitudinal members to compare the structural performance with inner longitudinal configurations. Analytical expressions were implemented in optimization studies to determine efficient and robust optimization techniques and optimize the inner and outer longitudinal configurations with respect to mass. Results indicate that the finite element predictions of axial stiffness and global buckling loads correlate with traditional mechanics equations, but overestimate the capacity demonstrated in previously published experimental results. The buckling modes predicted by finite element predictions correlate with traditional mechanics and experimental results, except when the local and global buckling loads coincide. The analytical expressions derived from mechanics to predict local buckling underestimate the constraining influence of the helical members, and therefore underestimate the local buckling capacity. The optimization analysis indicates that, in the specified design space, the structure with outer longitudinal members demonstrates a greater strength-to-weight ratio than the corresponding structure with inner longitudinal members by sustaining the same loading criteria with 10% less mass.

The diploma thesis focuses on programming in the Matlab for the Raspberry Pi 3 platform. For the purpose of the presentation, there are two applications designed for Raspberry Pi that are using available hardware, camera and servos. The first application serves as colour object detecting and accurate tracking by using camera calibration. The second application serves as a face detection and recognition. These applications are implemented by modern methods and knowledge of computer vision. Tracking of the objects and face recognition are verified by an experiment that reveals the accuracy of the used methods.