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Resistance to Bacillus thuringiensis (Bt) cotton, Gossypium hirsutum, is associated with fitness costs in pink bollworm, Pectinophora gossypiella. Previous work has demonstrated that costs of resistance are induced by environmental factors including insecticidal cotton phytochemicals such as gossypol. This study (1) tested the hypothesis that the presence of toxic phytochemicals would increase the dominance and magnitude of fitness costs associated with genes conferring resistance to the Bacillus thuringiensis (Bt) toxin, (2) coupled a selection experiment and simulation modeling to evaluate the fitness cost of resistance to Bt in pink bollworm and (3) used laboratory strains containing susceptible and resistant genotypes to test the hypothesis that gossypol accumulates more readily in pink bollworm with Bt resistance alleles, and that this gossypol accumulation reduces fitness. To test hypothesis (1), larval survival and weight were measured in two independent strains of pink bollworm, Pectinophora gossypiella, reared on diet containing the cotton phytochemicals gossypol and cyclopropenoid fatty acid, alone, or in combination. Cotton phytochemicals increased the dominance and magnitude of fitness costs associated with cadherin-based resistance to Bacillus thuringiensis (Bt) toxin. Gossypol and cyclopropenoid fatty acid combined had a more detrimental effect on fitness of pink bollworm than either compound alone. To test hypothesis (2), two pink bollworm strains fed synthetic diet were monitored over 30 generations to test the hypothesis that costs associated with Bt resistance would result in a decline in the frequency of resistance. A decrease in resistance allele frequency did occur in both strains and costs affecting each resistant genotypes were estimated. To test hypothesis (3), two strains of pink bollworm were fed on diet containing gossypol and on diet without gossypol, and gossypol accumulation in tissues of genotypes was measured. In both strains, significantly more gossypol accumulated in genotypes containing at least one resistance allele and gossypol accumulation was additive to dominant. In both strains, an increase in the dominance or magnitude of costs affecting larval weight was observed on gossypol diet, and the change in the magnitude of costs was positively associated with gossypol absorption. In one strain, the presence of gossypol increased survival costs but only in the genotype with the highest gossypol absorption. The mutation conferring resistance to the Bt toxin Cry1Ac is found in the cadherin-encoding region in three lepidopeteran pests (Helicoverpa armigera, Heliothis virescens and Pectinophora gossypiella) including the pink bollworm. Cadherin proteins had been hypothesized to play a role in maintaining the integrity of the insect midgut epithelial tissue. Taken together, these results support the hypothesis that cadherin proteins do contribute to gut integrity, toxic plant phytochemicals accumulate more readily in pink bollworm with resistance alleles, and that such accumulation increases the dominance and magnitude of fitness costs.

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / The proliferation of box mistletoe Amyema miquelii in eucalypt woodlands of south-eastern Australia may have resulted from the suppression of canopy fires, a reduction in herbivory by possums, and through environmental change, an improvement in conditions for mistletoe dispersal and establishment. In the Mount Lofty Ranges (MLR), South Australia, box mistletoe is often seen in high numbers in pink gum Eucalyptus fasciculosa woodlands. The following dissertation investigated box mistletoe dispersal and establishment by Mistletoebirds Dicaeum hirundinaceum in a pink gum woodland. The broad aims of the study were to advance our theoretical knowledge of mistletoe dispersal ecology, to understand why pink gum woodlands are more susceptible to mistletoe infection, and to increase the amount of ecological information available to land managers. A survey of box mistletoe and its Eucalyptus hosts in reserves of the MLR region revealed that almost a third of all pink gums were infected with box mistletoe. Individual pink gums with less foliage cover surrounding their canopy were more likely to host box mistletoe, suggesting canopy access for Mistletoebirds may influence the susceptibility of pink gums to mistletoe infection. Woodland type was more influential than fragmentation and edge effects in determining mistletoe presence, indicating a variation in host specificity across Eucalyptus species. The results of this survey indicated that further examination was required on Mistletoebird behaviour and mistletoe establishment success. Two aspects of Mistletoe bird ecology were examined: the influence of their movement patterns on the spatial dynamics of mistletoe dispersal, and their foraging behaviour. Mistletoebirds had home ranges of around 20 ha, and used small core areas (1 ha) of high mistletoe infestation more frequently than areas with lower mistletoe abundance. Modelling of mistletoe seed shadows indicated that the majority of mistletoe seeds (approx. 70%) would be deposited within 100 m of a parent plant. Consistent with this, seed rain modelling showed that mistletoe seed rain was aggregated, with birds dispersing large amounts of seed (> 66 000/ ha) in areas with higher mistletoe infestation levels. This indicated that the movements of mistletoe dispersers promote mistletoe aggregation not only at the scale of an individual tree, but also at a landscape scale. From a management perspective, the results indicated that the removal of mistletoes from single trees may have only short-term results, as reinfection from neighbouring host trees is likely. The attractiveness of pink gums to Mistletoebirds was a function of tree size, mistletoe crop size and tree access. Mistletoebirds preferred to forage in taller trees with a larger mistletoe crop size and which had greater canopy access, and Mistletoebirds most often alighted on dead pink gum when visiting a tree. The results support the notion that woodland dieback may improve conditions for mistletoe dispersal by allowing favourable habitat for Mistletoebirds, by increasing canopy access and by providing more perch sites. Dieback will also reduce mistletoe establishment, however, through a loss of suitable live host branches. The high frequency of box mistletoe infection in pink gum woodlands could also be explained by differences in establishment of box mistletoe between eucalypt species. A mistletoe establishment experiment demonstrated that establishment was significantly higher on pink gums than on E. porosa and E. camaldulensis, and that mistletoes established on pink gums were larger and had a greater number of leaves. The differences probably lay in underlying differences in host physical and chemical defences, and subsequent relative success of mistletoes to establish a functional haustorium. The dispersal syndrome of box mistletoe as described in this study is suitable and perhaps facilitated in the contemporary fragmented environment in a number of ways. These include an ability to concentrate their feeding and breeding activities in small areas of remnant vegetation, greater manoeuvrability between trees isolated by clearing, and easier access to the canopies and perch sites of individual trees in deteriorated woodlands. Priority research stemming from this study should include studies into the underlying causes of pink gum dieback, an experiment to test whether canopy die back directly results in more frequent visitation by Mistletoebirds, an examination of Mistletoebird movements in areas with low mistletoe abundance, and continued monitoring of mistletoe abundance and tree condition as established in this dissertation. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1285516 / Thesis (Ph.D.) -- University of Adelaide, School of Earth and Environmental Sciences, 2007

Title page, table of contents and abstract only. The complete thesis in print form is available from the University of Adelaide Library. / The proliferation of box mistletoe Amyema miquelii in eucalypt woodlands of south-eastern Australia may have resulted from the suppression of canopy fires, a reduction in herbivory by possums, and through environmental change, an improvement in conditions for mistletoe dispersal and establishment. In the Mount Lofty Ranges (MLR), South Australia, box mistletoe is often seen in high numbers in pink gum Eucalyptus fasciculosa woodlands. The following dissertation investigated box mistletoe dispersal and establishment by Mistletoebirds Dicaeum hirundinaceum in a pink gum woodland. The broad aims of the study were to advance our theoretical knowledge of mistletoe dispersal ecology, to understand why pink gum woodlands are more susceptible to mistletoe infection, and to increase the amount of ecological information available to land managers. A survey of box mistletoe and its Eucalyptus hosts in reserves of the MLR region revealed that almost a third of all pink gums were infected with box mistletoe. Individual pink gums with less foliage cover surrounding their canopy were more likely to host box mistletoe, suggesting canopy access for Mistletoebirds may influence the susceptibility of pink gums to mistletoe infection. Woodland type was more influential than fragmentation and edge effects in determining mistletoe presence, indicating a variation in host specificity across Eucalyptus species. The results of this survey indicated that further examination was required on Mistletoebird behaviour and mistletoe establishment success. Two aspects of Mistletoe bird ecology were examined: the influence of their movement patterns on the spatial dynamics of mistletoe dispersal, and their foraging behaviour. Mistletoebirds had home ranges of around 20 ha, and used small core areas (1 ha) of high mistletoe infestation more frequently than areas with lower mistletoe abundance. Modelling of mistletoe seed shadows indicated that the majority of mistletoe seeds (approx. 70%) would be deposited within 100 m of a parent plant. Consistent with this, seed rain modelling showed that mistletoe seed rain was aggregated, with birds dispersing large amounts of seed (> 66 000/ ha) in areas with higher mistletoe infestation levels. This indicated that the movements of mistletoe dispersers promote mistletoe aggregation not only at the scale of an individual tree, but also at a landscape scale. From a management perspective, the results indicated that the removal of mistletoes from single trees may have only short-term results, as reinfection from neighbouring host trees is likely. The attractiveness of pink gums to Mistletoebirds was a function of tree size, mistletoe crop size and tree access. Mistletoebirds preferred to forage in taller trees with a larger mistletoe crop size and which had greater canopy access, and Mistletoebirds most often alighted on dead pink gum when visiting a tree. The results support the notion that woodland dieback may improve conditions for mistletoe dispersal by allowing favourable habitat for Mistletoebirds, by increasing canopy access and by providing more perch sites. Dieback will also reduce mistletoe establishment, however, through a loss of suitable live host branches. The high frequency of box mistletoe infection in pink gum woodlands could also be explained by differences in establishment of box mistletoe between eucalypt species. A mistletoe establishment experiment demonstrated that establishment was significantly higher on pink gums than on E. porosa and E. camaldulensis, and that mistletoes established on pink gums were larger and had a greater number of leaves. The differences probably lay in underlying differences in host physical and chemical defences, and subsequent relative success of mistletoes to establish a functional haustorium. The dispersal syndrome of box mistletoe as described in this study is suitable and perhaps facilitated in the contemporary fragmented environment in a number of ways. These include an ability to concentrate their feeding and breeding activities in small areas of remnant vegetation, greater manoeuvrability between trees isolated by clearing, and easier access to the canopies and perch sites of individual trees in deteriorated woodlands. Priority research stemming from this study should include studies into the underlying causes of pink gum dieback, an experiment to test whether canopy die back directly results in more frequent visitation by Mistletoebirds, an examination of Mistletoebird movements in areas with low mistletoe abundance, and continued monitoring of mistletoe abundance and tree condition as established in this dissertation. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1285516 / Thesis (Ph.D.) -- University of Adelaide, School of Earth and Environmental Sciences, 2007

Doctor of Philosophy (PhD) / The Flesh Browning (FB) disorder of ‘Cripps Pink’ apples presents a significant threat to the established market identity of the ‘Cripps Pink’ apple in Australian and export markets. Climatic conditions during fruit growth and development predispose ‘Cripps Pink’ apples to developing the FB disorder during storage. The FB disorder can be classified into two distinct disorders based on their physiological and structural differences and by seasonal climatic conditions. The diffuse type of FB (DFB) is a chilling injury, occurring in districts or seasons accumulating less than 1100 growing degree days (GDD) above 10oC between full bloom and harvest. In these climatic conditions, ‘Cripps Pink’ apples have delayed postharvest ethylene production. Diffuse FB effects fruit cortex tissue and is characterised as cellular collapse. Storing fruit at 3oC can reduce the incidence of DFB. The radial type of FB (RFB) is primarily a senescent disorder, occurring in districts or seasons accumulating greater than 1400 GDD above 10oC between full bloom and harvest. In these climatic conditions, postharvest ethylene production is not delayed. Radial FB affects the cells adjacent to the vascular tissue of the fruit and is characterised by damaged cell walls. Storing fruit at 1oC can reduce the incidence of RFB. Harvest maturity and the level of CO2 in the storage atmosphere are additive influences on the development of RFB. Seasons or districts accumulating more than 1700 GDD have a very low risk for developing RFB. Seasonal climatic conditions can provide a guide for predicting the risk of developing RFB and DFB during storage.

Thesis (MScAgric)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: An investigation into the effects of different mulches on the root environment, encompassing physical, chemical and biological factors of the soil, on two different soil types was done in the form of a field trial on ‘Cripps’ Pink’ apples. Three organic mulches were tested: compost, vermi-castings and woodchips, as well as an inorganic mulch, geotextile fabric, and were compared against clean cultivation. The organic treatments resulted in improved physical conditions (lower bulk densities) in the heavier soil, as well as, a reduction in temperature fluctuations and a general increase in soil temperatures during the seasons, in both sites. The geotextile fabric treatment resulted in increased soil moisture levels in the top 40 cm, predominantly in the heavier soil. The compost treatment resulted in high soil moisture levels in the top 40 cm only in the lighter soil. The vermi-castings treatment achieved superior results in terms of changing the nutrient status of the heavier soil. It resulted in significantly higher pH, P (phosphorus), N (nitrogen), K (potassium), Mg (magnesium), Zn (zinc), Mn (Manganese), B (boron), as well as the cation exchange capacity and some exchangeable cations, such as, Na+ (sodium ions), K+ (potassium ions) and Mg+ (magnesium ions). The compost treatment resulted in significantly higher Ca (calcium) and Ca+ (calcium ions) in the heavier soil compared to the other treatments. The organic mulches, including the woodchips treatment, consistently resulted in higher mineral levels and therefore performed the best in this regard and did so in the heavier soil. In contrast to the heavier soil, none of the treatments were successful in ameliorating the nutrient status of the lighter soil, with the exception however of the increased percentage C as a result of the compost and vermi-castings treatments. The compost treatment realised consistently higher mychorrizael colonization in both sites, however, not always significantly higher than the other treatments. The vermi-castings treatment realised consistently lower plant parasitic nematodes numbers. Higher free-living nematodes were also frequently realised during both seasons and in both sites. The organic mulches therefore proved promising with regard to soil biota. The organic treatments, with the exception of the vermi-castings treatment, resulted in improved root number and distribution in the heavier soil. The vermi-castings treatment resulted in a superior root environment and did not need to enhance its root system in order to achieve good fruit yield and quality. In contrast, the geotextile fabric treatment performed better in this regard in the lighter soil. The geotextile fabric treatment also achieved the lowest weed counts, quantified as winter weeds, in both sites. Yield efficiency, in the heavier soil, and the incidence of sunburn in both sites, were influenced by mulching. In the heavier soil, the woodchips treatment resulted in the highest yield efficiency and the compost treatment consistently resulted in the highest incidence of sunburn. In the lighter soil the control treatment resulted in the highest incidence of sunburn. The vermi-castings treatment consistently resulted in lower incidences of sunburn. Due to the limited quantification of irrigation in this trial, the consequence of irrigation on different mulches was not evaluated and should be considered for future research. / AFRIKAANSE OPSOMMING: ‘n Ondersoek na die effek van verskillende deklae op die wortelomgewing, insluitende fisiese, chemiese en biologiese grond faktore, is uitgevoer as ‘n veldproef of twee verskillende grondtipes, op ‘Cripps’ Pink’ appels. Drie organiese deklae is ge-evalueer naamlik: kompos, ‘vermi-castings’ en houtspaanders, asook ‘n anorganiese geotekstiel materiaal deklaag, en vergelyk met ‘n kontrole van skoon bewerking. Die organiese behandelings het verbeterde fisiese kondisies (laer bulkdigtheid) in die swaarder grond, asook ‘n verlaging in temperatuur fluktuasies en algemene verhoging in grondtemperature gedurende die seisoene in beide persele tot gevolg gehad. Die geotekstiel behandeling het verhoogde grondvog-vlakke in die boonste 40 cm in beide persele tot gevolg gehad, alhoewel dit meer prominent in die swaarder grond was. In sanderige grondperseel, het die kompos behandeling hoër grondvog-vlakke in die boonste 40 cm getoon as die ander behandelings. Die ‘vermi-castings’ behandeling het die beste resultate in terme van verbetering van nutrient- vlakke in die swaarder grond behaal. Dit het ‘n betekenisvol hoër pH, P (fosfaat), N (stikstof), K (kalium), Mg (magnesium), Zn (sink), Mn (mangaan), B (boron), katioon uitruilings kapasiteit en sommige uitruilbare katione soos , Na+ (natrium ione), K+ (kalium ione) en Mg+ (magnesium ione) as die ander behandelings in die swaarder gehad. Die kompos behandeling het betekenisvol hoër Ca (kalsium) en Ca+ (kalsium ione) in die swaarder grond getoon. Die organiese behandelings, insluitend die houtspaander behandeling, het dus in die geval, konstant die beste resultate te opsigte van hoër nutriënt vlakke in die swaarder grond getoon. Inteenstelling met die swaarder grond, het geen behandling daarin geslaag om die grondvoedingstatus van die sanderige grond te verbeter nie, met uitsondering die verhoogde persentasie C as ‘n resultaat van die kompos en ‘vermi-castings’ behandelings. Die kompos behandeling het konstant hoër mychorriza-kolonisasie teweeg gebring in beide persele, alhoewel nie altyd betekenisvol hoër as die ander behandelings was nie. Die ‘vermicastings’ behandeling het konstant ‘n laer persentasie plant-parasitiese nematodes getoon. Meer vry-lewende nematodes het ook gereeld oor die totale vier jaar wat die volledige proef gestrek het, op beide persele, voorgekom. Die organiese deklae toon dus belowende resultate in terme van biota. Die organiese behandelings, met uitsondering van die ‘vermi-castings’ behandeling, het verhoogte wortel ontwikkeling en -verspreiding in die swaarder grond tot gevolg gehad, Die ‘vermi-castings’ behandeling het ‘n besondere goeie wortelomgewing geskep en ‘n verbetering in die wortelstelsel om ‘n goeie opbrengs en kwaliteit te behaal, was nie nodig nie. Daarinteen het die geotekstiel behandeling beter resultate in die meer sanderige grond behaal. Die geotekstiel behandeling het ook die laagste onkruidstand in beide persele gehad. Opbrengs, in die swaarder grond, en die voorkoms van sonbrand in beide persele, is beïnvloed deur die dekgewasse. In die swaarder grondperseel het die houtspaanders behandeling die hoogste opbrengs getoon en die kompos behandeling, konstant die hoogste voorkoms van sonbrand. In die ligter, sanderige grondperseel, is die hoogste sonbrand voorkoms gemeet in die kontrole behandeling. Die ‘vermi-castings’ behandeling het die laagste sonbrand voorkoms getoon. Weens die beperkte kwantifisering van die besproeiing in die proef, is die gevolge van besproeiing op verskillende deklae nie ondersoek nie en behoort dit oorweeg te word in toekomstige navorsing.

A vida útil do filé de salmão fresco é em grande parte limitada pela deterioração microbiana, proteolítica e oxidativa. Esse trabalho visou estudar o efeito do filme de quitosana adicionado ou não do extrato de resíduo agroindustrial de pimenta rosa (Schinus terebinthifolius Raddi) sobre a vida útil e qualidade de salmão do Atlântico (Salmo salar) fresco, embalado em atmosfera modificada (AM, 100% CO2), durante 28 dias. Os filés de salmão (± 300 g) com 11 dias post mortem sem pele e sem ossos foram embalados em 100% de dióxido de carbono (CO2), submetidos aos tratamentos: sem filme (TC), com filme de quitosana (TFQ) e com filme de quitosana adicionado do extrato de resíduo agroindustrial de pimenta rosa (TFQPR), armazenados a 2 ± 1° C e sem exposição à luz por 0 (11 dias), 7, 14, 21 e 28 dias. Após cada período de armazenamento, nove bandejas de cada tratamento foram analisadas de acordo com parâmetros físico-químicos (pH, cor, capacidade de retenção de água (CRA), perfil de textura (PT), bases voláteis totais (BVT), trimetilamina (TMA), estabilidade oxidativa, perfil de ácidos graxos (PAG), degradação de ATP e seus catabólitos), microbiológicas (microrganismos aeróbios mesófilos e psicrotróficos, bactérias láticas, coliformes totais e termotolerantes) e sensoriais. O estudo foi conduzido por um delineamento inteiramente casualizado com arranjo fatorial (3 tratamentos x 5 tempos de armazenamento), considerado como parcela o filé de salmão embalado em atmosfera modificada, com 3 repetições. Os dados foram analisados no ambiente R, a fim de verificar o efeito de tempo, tratamento e tempo x tratamento. Durante o armazenamento, o pH oscilou para os três tratamentos. Observou-se a descoloração dos filés de salmão devido à oxidação dos carotenoides astaxantina e cantaxantina, presentes no músculo do pescado. Ao longo do armazenamento, CRA foi reduzida, influenciando no perfil de textura das amostras. Os filés de salmão tornaram-se mais macios devido à proteólise muscular e a ação de microrganismos deteriorantes presentes. Os dados de oxidação lipídica foram baixos e não influenciou no PAG do salmão - considerando que o salmão é uma boa fonte de ácidos graxos poli-insaturados da série ômega-3. Os microrganismos deteriorantes analisados aumentaram ao longo do armazenamento, porém não ultrapassaram os limites recomendados pela ICMSF, bem como a contagem dos microrganismos patógenos. A análise sensorial permitiu verificar as mudanças nos filés de salmão ao longo do armazenamento, porém não houve diferença significativa entre os tratamentos. Em conclusão, o filme ativo foi eficiente na manutenção da qualidade e vida útil dos filés de salmão embalados com AM durante 28 dias de armazenamento, em comparação com o grupo controle (TC). / Shelf-life of the fillet of fresh salmon is greatly limited by microbial, proteolytic and oxidative deterioration. This research aimed to study the effect of chitosan active films and the addition of agro-industrial residue of pink pepper (Schinus terebinthifolius Raddi) on this film on fresh Atlantic salmon (Salmo salar) shelf-life and quality packaged in modified atmosphere packaging (MAP, 100% CO2) during 28 days. Skinless and boneless salmon fillets (± 300 g) with 11 days post mortem were packaged in 100% carbon dioxide (CO2) MAP according to three treatments: without chitosan film (TC), with chitosan film (TFQ) and with chitosan film added with agro-industrial residue of pink pepper (TFQPR), stored at 2 ± 1° C and under dark condition for 0 (11 days),7, 14, 21, and 28 days. After each retail day, 9 trays of each treatment were analyzed according to physical-chemical (pH, color, water holding capacity (WHC), texture profile (TP), total volatile basic nitrogen (TVB-N), trimethylamine (TMA), oxidative stability, fatty acid profile (FAP), and ATP and ATP-catabolites quantification), microbiological (the content of mesophilic, psychrotrophic, lactic, thermotolerant bacteria and total coliform), and sensory parameters. This research used Completely Randomized Design (CRD) with a factorial arrangement (3 treatments x 5 five storage time), considering the packaged salmon fillet as a research unity, with 3 repetitions. Data was analyzed on R environment, in order to verify time, treatment and time x treatment effects. During storage, pH oscillated for the three treatments. The salmon fillets discolored due to the carotenoids astaxanthin and canthaxanthin oxidation, which are present in fish muscle. Along storage time, WHC reduced, influencing on sample texture profile. Salmon fillets softened, which is possibly resulting from muscle proteolysis and from spoilage bacteria action. Lipid oxidation data were low and did not influence on salmon FAP - considering that salmon is a good source of omega-3 poly-unsaturated fatty acid. Fish spoilage bacteria increased along storage, but it was not higher than the legal limit established by ICMSF, as well as pathogen bacteria. Sensory analysis revealed overall changing on salmon fillets during storage. In conclusion, active film was efficient in the maintenance of quality and shelf-life of MAP-packaged salmon fillets during 28 days of storage, compared to control group (TC).

The pigments responsible for pink or red discoloration in cooked vacuum-packaged meat products {bratwurst (pork and beef), ground pork, and pork roasts} were investigated. In bratwurst, this study attempted to characterize the red pigment that appears upon refrigerated storage, and to determine the effect of pH, cooking and storage temperature, and sodium lactate on incidence of red discoloration. Myoglobin was identified in the exudate of samples with red discoloration. Myoglobin levels of cooked products were significantly lower in samples of low initial pH (5.5) or cooked to higher than normal internal temperature (74°C). Red discoloration was associated with microbial growth. Frozen samples had no red discoloration after 4 weeks storage. Microbial plate count and incidence of red discoloration were lower in samples of lower pH (5.5 vs 5.8 or 6.2), samples cooked to higher internal temperature (74° vs 68°C), and samples containing 3% sodium lactate. Further, the effects of microbial growth in raw materials (ground pork) on cooked pork color were investigated. In two trials with sow meat held aerobically at 2°C for 3 weeks, microbial load reached spoilage levels (107 cfu/g), pH increased to 6.46, and samples cooked to 71°C had red exudate, shown by absorption spectroscopy to contain myoglobin and cytochrome c. Samples cooked to 82°C received high panel ratings for red color, due to red, flocculent precipitate in exudate, but samples containing undenatured myoglobin levels received low panel ratings. In sow meat held frozen or vacuum packaged at 2°C, pH after 3 weeks was 6.03 and 6.18, and plate counts were 104 and 107, respectively, but exudates after cooking were much less red. In five trials with fresh U. S. #1 pork legs, plate counts also reached 107 cfu/g by 3 weeks storage, and pH increased from 5.99 to 6.37, but cooked samples were not red. Higher myoglobin levels in sow meat probably accounted for the red color and the high level of undenatured myoglobin remaining after cooking of high pH, spoiled samples. Finally, pink or red discoloration was investigated in the cooked U. S. #1 pork roasts. Myoglobin was the pigment responsible for pink color in pork roasts cooked to 65°C. Roasts cooked to 82°C had gray internal color after cooking, but developed pink internal color after refrigerated storage. Reflectance spectra of pink slices from roasts, cooked to 82°C, then stored for 12 days at 2°C, were charactetistic of denatured globin hemochromes or related non-nitrosyl hemochromes.

BANG! is a thesis paper presented in conjunction with a visual art show that is made up of paintings, sculptures, and site specific installations. Primary ideas explored with the body of work are those of growth and expansion, energy, contradiction, excess, collection, play, drama, and nostalgia, specifically relating to color relationships and physical material.