This research examined the fire history of ponderosa pine (Pinus ponderosa Douglas ex P. Lawson & C. Lawson) forests in northwestern New Mexico. The study area included three sites in the Zuni Mountains of Cibola National Forest and one site along the boundary of El Malpais National Monument. I crossdated over 800 fire scars on 75 samples to reconstruct spatial and temporal characteristics of historic wildfire regimes. The Weibull Median Interval, Weibull Modal Interval, and Mean Fire Interval ranged from five to eight years across all sites and percent-scarred classes (all fires, 10% scarred, and 25% scarred) and indicated that low-severity wildfires occurred frequently in the study area during the period 1700 to 1880. Wildfires were historically driven by climatic variability. Superposed Epoch Analyses revealed that wetter conditions typically occurred one to three years prior to a fire event and were followed by drought during the fire year. No relationship was found between the Pacific Decadal Oscillation (PDO) and wildfire occurrence. These findings implied that shorter-term fluxes between wet and dry conditions, rather than longer-term climatic variability, were historically most conducive to fire occurrence. Fire frequency decreased suddenly in the late 19th century across the study area, and results indicated that fire has been absent at all sites since the 1920s. Anthropogenic disturbances including livestock grazing, timber harvesting, and fire suppression likely explain observed differences between historic and contemporary wildfire regimes in the Zuni Mountains. This research has important implications for forest management. In ponderosa pine forests of the southwestern United States, land managers often aim to restore historic ecological conditions. The reintroduction of a frequent, low-severity wildfire regime might restore some ecological patterns and processes, but given the strong legacy of human disturbances and the influences of human-induced climate change, a complete return to historic conditions may be neither possible nor desired.

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Beginning in the late 1930s, fire exclusion has drastically altered the vegetation dynamics of the southern Appalachian Mountains. Extremely low fire frequency has allowed for more shade-tolerant species to invade once predominantly open forests and has made it almost impossible for fire-dependent species to establish on a site. One such species is the endangered Peters Mountain mallow (Iliamna corei Sherff.) located on Peters Mountain in The Nature Conservancy s Narrows Preserve in Giles County, Virginia. This paper focuses on the fire history and stand dynamics of Peters Mountain and how fire exclusion has altered the forest composition. The historic fire frequency and successional changes discovered here may provide an insight into management strategies for the mallow. Seventy-nine fire scarred cross-sections were taken and aged to determine fire history dates and frequencies. Three 50x20 meter plots were set up on opposing aspects: northwest and southeast. The aspects were chosen at the direction of The Nature Conservancy personnel. All trees within were identified, cored and aged to determine species composition and the establishment dates of all trees. Fire history analysis revealed a mean fire interval of 2.48 years, a Weibull median fire interval of 2.18 years and a 25 percent scarred class mean fire interval of 12.5 years. Stand dynamic results show that Quercus montana has established on Peters Mountain prior to fire exclusion and remains the dominate species on the landscape. An increased number of fire intolerant species (including Acer rubrum, Sassfras albidum, Nyssa sylvatica) have been establishing on Peters Mountain during the decades of decreased fire frequency, suggesting a shift in forest composition. Frequent fires are suggested for mallow management and oak forest maintenance.

The molecular mechanisms underlying disease-resistance and drought-resistance in forest trees are not well understood. Linking variation in gene expression with genetic polymorphisms and with variations in disease- and drought-resistance phenotypes can provide information about these complex traits. We used real-time quantitative polymerase chain reaction (PCR) to detect variations in the expression of 88 disease- and drought-responsive genes within an association population of 354 loblolly pine trees (Pinus taeda L.). Using association genetics approaches, we then linked 3,938 single nucleotide polymorphisms (SNPs) in candidate genes with gene expression phenotypes to identify novel disease- and drought-responsive genes. To further examine differences in gene expression induced by drought, Fusarium circinatum (responsible for pitch canker disease), and drought F. circinatum, the expression of 114 genes identified through comparative and association genetics approaches was analyzed on a subset of 24 loblolly pine trees possessing a range of pitch canker- and drought-resistance phenotypes. Significant differences in the uninduced expression of all 88 genes measured on the association population were observed among loblolly pine trees. Principal component analysis showed that some variation within the association population could be accounted for by population substructure of geographic origin. Hierarchical clustering of genes based on uninduced expression did not consistently group together functionally similar genes probably because expression was collected on unstressed stem tissue. This was supported in the smaller expression study as correlations between expression values of genes in the same functional networks were usually stronger when induced by a treatment compared with correlations between the uninduced expression of genes in the control group. Gene expression frequently changed by up to 4-fold in response to one or more treatments, but PtMYB12 was the only gene that exhibited a statistically significant change in response to treatments. ANOVA analyses of gene expression controlling for pitch canker resistance and for water use efficiency phenotypes identified differentially expressed genes suggesting that they may be contributing to these phenotypes. Finally, association genetics approaches detected 101 significant associations between SNPs in 94 candidate genes potentially involved in stress responses and 27 gene expression phenotypes.

The role of fire in determining the structure and composition of many forested ecosystems is well documented (e.g. North American boreal forests; piñon-juniper woodlands of the western US). Fire is also believed to be important in temperate forests of eastern North America, but the processes acting here are less clear, particularly in xerophytic forests dominated by yellow pine (Pinus, subgenus Diploxylon Koehne) and oak (Quercus L.). In this study, I use dendroecological techniques to investigate fire history and vegetation dynamics of mixed pine-oak forests in the central Appalachian Mountains of Virginia. The study addresses three objectives: (1) develop a lengthy fire chronology to document fire history beginning in the late presettlement era and extending throughout the period of European settlement, industrialization and modern fire exclusion; (2) explore fire-climate relationships; and (3) investigate vegetation dynamics in relation to fire occurrence. The study was conducted on three study sites within the George Washington National Forest. I used fire-scarred cross-sections from yellow pine trees to document fire history. Fire-climate relationships were investigated for each study site individually and all sites combined using superposed epoch analysis (SEA). Fire-history information was coupled with dendroecological data on age structure to explore stand development in relation to fire occurrence. Results of fire history analysis reveal a long history of frequent fire with little temporal variation despite changes in land use history. Mean fire intervals (MFI) ranged from 3.7–17.4 years. The most important change in the fire regime was the initiation of fire suppression in the early twentieth century. Results of SEA show that periodic droughts may be important drivers of fire activity. Drought the year of fire was important at two of the three study sites and when all sites were combined. Results of age structure indicate that vegetation development was clearly influenced by fire. Frequent burning maintained populations of yellow pine throughout the period of study until fire suppression allowed fire-sensitive hardwood trees and shrubs to establish. It is clear from this study that continued fire suppression will likely result in fire-tolerant pines and oaks being replaced by more mesophytic trees and shrubs.

Turkish red pine is coastal tree and is a drought resistant pine that withstands more aridity and poor soils than most other timber species growing in the same climatic conditions. In Turkey, this species grows in southern and western Anatolia and is also found in the Marmara region. Drought results in a water deficit in plant tissues, which, in turn, can lead to an imbalance in the redox poise of plant cells, and thus inducing oxidative stress in plants. Resistance to conditions associated with oxidative-stress must, in part, rely on endogenous antioxidative defense mechanisms required to maintain cellular homeostasis. Glutathione is one of the major endogenous antioxidants in plants known to play an important role in plant defense mechanisms. Glutathione S-transferase (GST, EC 2.5.1.18) is a GSH dependent detoxifying enzyme in plants, which catalyzes the conjugation of GSH. In this study, we investigated the changes in cytosolic glutathione S-transferase enzyme activity using CDNB as substrate and total thiol amount in Pinus brutia Ten., related to the drought stress during four months, June to September. The osmotic pressure in the needles was also determined as an indirect measure of drought condition. Together with the increase in the temperature values from June to July, GST enzyme activity increased from 15,78 &plusmn / 1,36 &micro / moles min-1 mg protein-1 to 22,91 &plusmn / 1,99 &micro / moles min-1 mg protein-1 which was statistically significant. However in August, GST activity had fallen to 16,54 &plusmn / 1,61 &micro / moles/min/mg protein, which may be because of a local rainfall at the beginning of the August in the sampling area. In September, GST activity significantly increased with respect to June, in accordance with high temperatures. The total thiol amount was not changed significantly during the sampling period. Although there were statistically significant changes in osmotic pressure in the needdles collected during the same sampling period, it did not exactly correlated to the changes in GST activity.

More than half of the Pinaceae is including in genus Pinus covers the large parts of vegetation of northern hemisphere. The Anatolian Black Pine is one of the subspecies of European Black Pine, growing naturally as a widespread mid elevation species of Taurus, western Anatolian and northern Anatolian Mountains of Turkey. Although it is disputed that there are 5 varieties of Anatolian black pine but three of these are well recognized. These are Pinus nigra subsp. pallasiana var. pallasiana, Pinus nigra Arnold subsp. pallasiana var. pyramidata (pyrimidal black pine) and Pinus nigra Arnold subsp. pallasiana var. seneriana. To determine the genetic relationship between Anatolian black pine and its well recognized varieties, 3 different taxa of Anatolian black pine (well recognized varieties) were sampled in the natural range of species and non-coding trn regions of chloroplast DNA (cpDNA) were sequenced to assess the genetic structure of the species. Three sectors of trn region were examined. Analysis was assessed with using MEGA version 4.0 and Arlequin 2.000 softwares. Considering genetic diversity of three Anatolian black pine taxa with respect to trn regions and parsimonic sites, the result showed that P. nigra subsp pallasiana var seneriana was more polymorphic than other two taxa. Also, the most distant taxon that show differences in trn sequences when compared to other taxa was P. nigra subp pallasiana var pyramidata. The constructed phylogenetic tree showed that individuals of P.nigra subsp pallasiana var pyramidata were grouped together. However, other two taxa showed a dispersed allocation in the tree. This result indicates that var pyramidata was the most distant taxon. According to present study, there is no clear speciation between varieties and var pallasiana. The differences between them may be a result of mutation which may have occured in the genes coding for growth and form of Anatolian black pine.