Cardiovascular disease, mostly atherosclerosis, is responsible for one third of all deaths globally, rising to more than 50% in the Western World. Risk factors include smoking, diet, and familial history. Doppler ultrasound can provide estimates of blood velocity and wall shear rate. Clinically, maximum velocity is used to categorise patients for surgery, although Doppler velocity measurement is prone to errors and in need of validation. Wall shear stress—which can be derived from wall shear rate—plays a role in disease initiation and progression, although its clinical utility is unclear due to difficulties associated with its measurement. This thesis investigates the use of Doppler ultrasound as a tool to estimate blood velocity and wall shear rate. A simplified method for estimation of wall shear rate in healthy arteries is developed that uses spectral Doppler ultrasound. This method is based upon the theory of oscillatory flow in rigid pipes, requiring two measurements that are readily available with clinical ultrasound machines. This method is compared to a similar method based on colour flow imaging. The spectral Doppler method underestimated the theoretic value of wall shear rate by between 7 and 22%, with results varying between phantoms. Errors for the colour method were on average 35% greater. Test measurements from one healthy volunteer demonstrated that this method can be applied in-vivo. In more advanced stages of disease, peak velocity distal to a stenosis is of clinical interest and the simplified method for wall shear rate estimation is invalid. Steady flow in a series of simplified stenosis geometries was studied using a dual-beam Doppler system to obtain velocity vectors. These measurements were compared with data from an equivalent system that used particle image velocimetry (PIV) and was considered the gold standard. For Reynolds numbers at the stenosis throat of less than 800, flow remained laminar over the region studied, although distal flow separation did occur. For higher throat Reynolds numbers—corresponding to more severe stenoses or increased flow rates—asymmetric recirculation regions developed; the transition to turbulence occurred more proximally, with a corresponding reduction in stenotic jet and recirculation length. Qualitative agreement was observed in the velocity profile shapes measured using ultrasound and PIV at throat Reynolds numbers less than 800. Above this threshold the qualitative agreement between the velocity profiles became poorer as both downstream distance and the degree of stenosis increased. Peak axial velocity distal to the stenosis was underestimated, on average, by 15% in the ultrasound system. Estimation of shear rate remained difficult with both experimental techniques. Under a Newtonian approximation, the normalised wall shear stresses agree qualitatively. Under pulsatile flow conditions using an idealised flow waveform, superior qualitative agreement was observed in the velocity profiles at diastole than at systole. Similar to the steady flow behaviour, this agreement deteriorated with stenosis severity. The current generation of clinical ultrasound machines are capable of estimating the wall shear rate in healthy arteries. In the presence of significant arterial disease, errors in the peak velocity may result in mis-selection of patients for surgery, while estimation of the wall shear stress remains extremely problematic; particularly with identifying the wall location and measuring velocities close to the wall.

Dans cette thèse, nous analysons l'écoulement et les transferts thermiques convectifs dans des modèles géométriques d'échangeurs de chaleur multi-rangs de tubes à ailettes planes continues. Dans un premier temps, les phénomènes Aérauliques qui s'y développent sont mis en évidence par des mesures PIV et LDA. Une étude locale de la génération, du développement, de l'évolution spatiale etde la dissipation des enroulements tourbillonnaires produits dans l'échangeur est effectuée. Dans un second temps, des simulations numériques U-RANS sont réalisées et validées par comparaison de la structuration de l'écoulement et de la dynamique tourbillonnaire aux mesures expérimentales. Dansun troisième temps, l'influence de ces tourbillons sur le transfert thermique est mise en exergue. Les performances d'échange sont caractérisées selon une analyse de synergie entre le champ de vitesse, les gradients de vitesse et de température ainsi qu'en évaluant les irréversibilités thermoaérauliques produites dans l'écoulement. Dans un quatrième temps, une analyse de l'influence de la forme du tube sur les performances thermoaérauliques locales et globales de l'échangeur est effectuée selon le premier et le second principe de la Thermodynamique. Les transferts thermiques, les pertes visqueuses ainsique les taux de production d'entropie thermique et visqueuse dans le fluide sont évalués. Enfin une méthode d'optimisation géométrique globale basée sur l'analyse factorielle de TAGUCHI est utilisée pour sélectionner les paramètres les plus influents sur les performances thermoaérauliques globales dans l'optique d'une conception optimisée des surfaces d'échange pour une application à la climatisationferroviaire.

The goal of wind-waves research is to predict the waves field and its effect on the environment. That environment could be natural or imposed by human endeavour. The mechanism of wind generated waves is described in the present work as a wind-bulk flow interaction rather than as a mechanical process which only transfers the wind energy to the wave. In the light of this description, the generation and growth of surface waves are functions of the physical properties of the interface, density of the bulk flow perturbations and wind shear stress. While the present models for the prediction of surface growth and evolution show some consistence - in some cases - with observations that were conducted in laboratories and in real fields, the work presented in this thesis justifies and explains the inconsistency or contradictions in other cases between the observations and the predictions. Also, physical interpretations for observations, for example wave growth with fetch, are suggested in the present work. To illustrate the physical mechanism responsible for wave generation and growth under the effect of wind action, two approaches are used. The first involves studying the effect of the physical properties of the water surface on atmospheric input into the bulk and thus the effect on the formation and growth of capillary waves. The second involves studying the correlation between the wave formation and growth and the density of the bulk perturbations. Wide ranges of previous data are used to analyse the effect of the physical properties of the water surface on wave generation and growth mechanism for the first approach. Also, a group of experiments using the PIV system (Particle Image Velocimetry) were conducted to study the correlation between the wind speed, bulk flow evolution and wind-waves‟ generation and growth for the second approach. The main physical parameters which are responsible for the generation and growth of capillary waves are determined. The Ohnesorge number is modified to predict the generation and growth of surface waves. In the second part, additional physical parameters of the bulk flow are introduced to illustrate the correlation between the wind generated waves and bulk flow evolution. A new parameter is used to scale the transition of the bulk flow from laminar flow to turbulent flow or the transition of the water surface from an undisturbed surface to a fully disturbed surface. The history of wind-wave research is relatively short. Although there were basic developments in the 18th century, a concentrated effort really began as a result of the military imperative of the Second World War. These developments were however, largely empirical. A theoretical frame work began to develop with the studies of wind-wave generation in the last century. The present work is conducted to fill some gaps in wind generated surface waves research and to introduce new approaches to simplify understanding wind-waves field and its effect on the environment.

A series of experimental studies was performed to investigate two separate fluid impingement flow systems intended for removal of particles from a surface or deposition of particles onto a surface. One of these flow systems is generated using a nozzle that incorporates both tilted jets and suction to create what we call a "bounded vortex flow", consisting of an annular swirling jet and a wall-normal vortex with axial upflow into a suction outlet. The other flow system is generated by a combination of acoustic streaming and substrate heating from an ultrasonic source. The primary methods used in the study for flow field measurements included laser-induced fluorescence (LIF) and particle-image velocimetry (PIV). Thermocouples are utilized for gathering temperature information from the ultrasonic induced flow. For the bounded vortex flow, different jet/suction flow rates and different nozzle-substrate separation distances were examined. In the acoustic-generated flow system, different acoustic intensities and transducer-substrate separation distances and different choices of substrate material were examined. Both flow systems achieve high levels of shear stress on the impingement surface via a combination of flow oriented toward and/or away from the surface and via formation of vortex structures near the impingement surface. In the bounded flow configuration, the vortex flow is oriented with axis normal to the impingement surface, whereas in the acoustic-generated flow a series of vortex rings form with axes parallel to the impingement surface. For both flow fields, conditions are observed with high impingement surface shear stress that are well suited to particle removal from the impingement surface. However, as the variables controlling the flows are varied, other conditions are observed in which the flow fields become unstable, leading to oscillatory flows that generally have much smaller shear stress values on the impingement surface. The rate of fluid mixing, as characterized by upward and downward flows normal to the impingement surface, is also generally decreased after these flow transitions have occurred, implying that the unstable flows will be less suited for both particle deposition on and particle removal from the impingement surface.

Heart transplant is often considered the best treatment for prolonged left ventricular failure. However due to limited donor hearts and ineligibility of some patients to get a transplant, heart assist devices are considered a short/long term option to assist the failing ventricle. Numerous in vitro and animal trials are conducted to study the flow fields in artificial ventricles to avoid hemolysis and thrombosis but the effects of flow fields corresponding to the angle of LVAD insertion into the aorta are often neglected. This experiment uses 30, 60 and 90 degree glass models to signify different angles of the surgical end to side anastomosis. Particle Image Velocimetry is used to study the flow fields at the interface and determine optimum angle amongst the three to avoid blood trauma. Use of a 30 degree glass interface minimizes stagnation zone, recirculation patterns, flow reversal, high shear region and other flow irregularities which may lead to reduced hemolysis, thrombosis and blood trauma in a clinical setting.

This thesis project examined the interactive fluid dynamics between a blood pump and the univentricular Fontan circulation. 2-D particle image velocimetry (PIV) measurements were conducted on an idealized total cavopulmonary connection (TCPC) with an axial pump prototype in the inferior vena cava (IVC). Fluid velocity profiles were examined under various physiologic conditions for Fontan patients. The velocity profiles for all cases demonstrated the shunting of flow from the IVC toward the right pulmonary artery. A rotational component in the pump outflow was observed forcing flow to the periphery as compared to the flow profile without a pump present in the IVC. The inclusion of the pump provides a pressure rise of 3 to 9 mmHg. These results demonstrate the ability of the intravascular blood pump to support a Fontan circulation and support the continued optimization and development of the pump.

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.

This study aimed to understand the processes that govern free surface behaviour in depth-limited turbulent flows. Experimental data has shown that the turbulence properties at a point near the free surface relate directly to the properties of the free surface pattern. This would suggest a direct linkage between the free surface and the underlying turbulence field, but this cannot be true since the free surface pattern is strongly dynamic while the sub-surface turbulence field is relatively persistent. An oscillatory spatial correlation function was derived which explains the de-linkage, showing that the turbulence-generated surface pattern periodically inverts as it advects downstream. A model was developed, which shows that the observed free surfaces can be considered as an ensemble of overlapping but behaviourally independent oscillons. These are shown to influence a zone of fluid beneath the surface and invert at a frequency which is a function of the root-mean-square roughness height of the free surface. The spatial frequency of free surface oscillation relates strongly to the spatial frequency of turbulent structures, suggesting that the oscillon motion may form the trigger for near-bed bursting events. Given these relationships, it is proposed that measurement of the free surface behaviour may allow remote measurement of flow conditions. An acoustic wave probe was developed, which is able to remotely recover the key features of the water surface pattern. An array of such probes is proposed for the accurate measurement of temporal and spatial properties of turbulent free surfaces and hence the underlying bulk flow conditions.

Abstract An experimental study is conducted to determine the velocity fields, from development to steady state, in a square enclosure due to movement of a constant velocity lid using Particle Image Velocitmetry (PIV). Experiments were conducted with water, seeded with hollow glass sphere particles 10 microns in diameter, at three different lid velocities leading to Reynolds numbers in the high laminar to transitional range. Driven Cavity Flow is a classic fluid dynamics case often used for benchmarking of computational codes. Previous work has primarily focused on improving computational codes, experimental work is lacking and focused on obtaining steady state readings. The test cavity is 1 inch (25.4mm) high by 1 inch (25.4 mm) wide leading to an aspect ratio of 1.0. The depth is taken to be 5 (127mm) inches to reduce the three dimensional effects. Readings are taken from development to steady state allowing for a full spectrum of flow characteristics. PIV technique is successful in capturing the development of driven cavity flow. Circulation is shown to increase strength with time and Reynolds number. PIV capture and processing settings are determined. Keywords: Driven Cavity Flow, Particle Image Velocimeter (PIV)

O objetivo do presente estudo foi o de determinar em qual fase da onda de crescimento folicular são obtidas maiores taxas de recuperação e competência de oócitos obtidos por aspiração folicular (OPU) em novilhas cruzadas (Bos indicus x Bos taurus; Capítulo 1) ou em novilhas bubalinas (Bubalus bubalis), zebuínas (Nelore; Bos indicus) e taurinas (Holandês; Bos taurus; Capítulo 2). No Capítulo 1, 30 novilhas cruzadas foram submetidas à OPU aos 2 (D2), 4 (D4) e 6 (D6) dias após a emergência da onda de crescimento folicular (n=10/ grupo), em delineamento "cross-over" realizado em 3 réplicas. Os oócitos recuperados foram selecionados e os viáveis submetidos aos procedimentos de produção in vitro. Os dados foram analisados por ANOVA, utilizando o PROC MIXED, considerando os efeitos fixos de momento de OPU, de réplica e da interação entre eles, e o efeito aleatório de doadora. Foram produzidos maior taxa de blastocistos aos 6 dias pós-FIV, bem como maior número de blastocistos aos 9 dias pós-FIV e maior número de núcleos dos embriões eclodidos quando procedeu-se com aspiração no D2. Contudo, a OPU realizada no D6 também influenciou positivamente a taxa de blastocistos 6 dias pós FIV e não diferiu dos outros momentos com relação ao número de núcleos dos embriões eclodidos. No Capítulo 2, foram realizados 2 experimentos (1 e 2). No Experimento 1, foi avaliado o efeito de 50 mg de progesterona injetável na sincronização de emergência folicular em novilhas Nelore, Holandesas e bubalinas submetidas a protocolo de sincronização à base de progestágeno associado a 2 mg de benzoato de estradiol (n=10/ grupo). Os dados foram analisados por regressão logística, utilizando o PROC GLIMMIX. O momento da emergência folicular não foi afetado pela administração de progesterona injetável, ocorrendo entre 4,2 a 4,5 dias após o início do protocolo de sincronização para os três grupos genéticos. No Experimento 2, foram avaliados os efeitos de momento de sincronização da emergência folicular para a aspiração folicular (1, 3 ou 5 dias após a emergência) e de grupo genético (Nelore, Holandês e búfalo) na produção de embriões in vitro. Um total de 27 novilhas (9 de cada grupo genético, subdivididas nos 3 momentos de aspiração folicular) foram submetidas a 6 sessões de OPU, em delineamento "cross-over". Os oócitos recuperados foram selecionados e os viáveis submetidos aos procedimentos de produção in vitro. Os dados foram analisados por ANOVA, utilizando o PROC MIXED, considerando os efeitos fixos de momento de OPU, de grupo genético, de réplica e da interação entre eles, e o efeito aleatório de doadora. Os resultados demonstraram que novilhas Nelore apresentaram melhor eficiência para a produção de embriões in vitro do que as novilhas Holandesas ou bubalinas, e que a aspiração folicular realizada em diferentes momentos da onda folicular sincronizada não influenciou a produção in vitro. / The objective of the present study was to determine in which phase of follicular wave higher recovery rates and better oocyte competence can be obtained by ovum pickup (OPU) in crossbred (Bos indicus x Bos taurus; Chapter 1) or buffalo (Bubalus bubalis), Nelore (Bos indicus) and Holstein heifers (Bos taurus; Chapter 2). In Chapter 1, 30 crossbred heifers were submitted to OPU on days 2 (D2), 4 (D4) or 6 (D6) after follicular wave emergence (n=10/ group), in a cross-over design performed in 3 replicates. Oocytes recovered were selected and those viable were submitted to in vitro embryo procedures. Data were analyzed by ANOVA, using PROC MIXED, in which time of OPU, replicate and interaction between these factors were considered as fixed effects, and donor was considered as a random effect. Higher blastocyst rate (6 days after IVF), number of blastocysts (9 days after IVF), and number of nuclei of hatched embryos were produced when OPU was done on D2. However, OPU on D6 also produced a higher blastocyst rate (6 days after IVF), and did not differ from other times of OPU concerning number of nuclei of hatched embryos. In Chapter 2, two experiments were performed. In Experiment 1, the effect of 50 mg of injectable progesterone was evaluated in the synchronization of follicular wave emergence of Nelore, Holstein, and buffalo heifers submitted to a progestin plus 2 mg of estradiol benzoate protocol (n=10/ group). Data were analyzed by logistic regression, by PROC GLIMMIX. The time of follicular wave emergence was not affected by administration of injectable progesterone. Besides, follicular wave emergence occurred between 4.2 and 4.5 days after the beginning of synchronization protocol for all genetic groups. In Experiment 2, the effects of time of OPU relative to follicular wave emergence (1, 3 or 5 days) and of genetic group (Nelore, Holstein, and buffalo) on in vitro embryo production were evaluated. A total of 27 heifers (9 of each genetic group, assigned in the 3 times of OPU) were submitted to 6 OPU sessions, in a cross-over design. Oocytes recovered were selected and those viable were submitted to in vitro embryo procedures. Data were analyzed by ANOVA, using PROC MIXED, in which time of OPU, genetic group, replicate and interaction between these factors were considered as fixed effects, and donor was considered as a random effect. Results demonstrated that Nelore heifers showed a better efficiency for in vitro embryo production than Holstein or buffalo heifers. Additionally, OPU performed at different times of synchronized follicular wave did not influence in vitro embryo production.