Population development in Sweden since 1960 Abstract This diploma thesis deals with the population development in Sweden between 1960 and 2018. The main goal of this work is to evaluate in the context of selected demographic characteristics the course of demographic changes that appeared in the country after 1960. The result is changes in the level of demographic reproduction, which are referred to as the second demographic transition. From the 1960s to the present, Sweden has seen an increase in the average age at first marriage, an increase in the average age of the mother giving birth and an increase in the number of divorces. During the observed period, the Swedish population aged, the share of the pre-productive component of the population decreased, while the share of the post-productive component in the population increased. The development of total fertility in Sweden is often compared to a roller coaster due to its specific fluctuation. From 1960 to 2018, Sweden saw a decline in mortality rates, especially in the older age groups and up to one year of age, which was reflected in the values of life expectancy at birth that grew for both men and women. Sweden is inspiring for other European countries with its gender-neutral family and social policy. Keywords: population development, Sweden, second...

Wohnbaulandprognosen werden oft noch sehr normativ aus dem Trend der Bevölkerungsentwicklung und dem steigenden Wohnflächenkonsum der Einwohner erstellt. Unter den Bedingungen des demografischen Wandels gerät diese Praxis jedoch in erhebliche Schwierigkeiten. Warum sind Wohnbaulandausweisungen noch notwendig, wenn sowohl die Einwohnerzahl sinkt als auch die Zahl leer stehender Wohnungen zunimmt? Handelt es sich hier vorrangig um steigende Flächenansprüche der Haushalte oder um Effekte veränderter Lebensweisen? In Schrumpfungsregionen lassen schnelle Einschätzungen zunächst keinen zusätzlichen kommunalen Flächenbedarf für Wohnen erkennen und dennoch nimmt die Flächenneuinanspruchnahme für Wohnzwecke weiter zu. So wird deutlich, dass die herkömmlichen methodischen Ansätze für kommunale Wohnbaulandprognosen nicht mehr ausreichen und weitere Einflussfaktoren der Flächenentwicklung berücksichtigt werden müssen. Neue Modelle, welche die kleinräumigen Nachfrageentwicklungen sowie die Divergenzen zwischen Angebots- und Nachfragestruktur abbilden können, sind noch rar. Der folgende Beitrag zeigt, welche methodischen Stärken und Schwächen kommunale Prognoseansätze haben und stellt wichtige Grundgedanken der kommunalen Wohnungsprognosen des Leibniz-Instituts für ökologische Raumentwicklung (IÖR) vor.

The development of shape in multicellular organisms has intrigued human minds for millenia. Empowered by modern genetic techniques, molecular biologists are now striving to not only dissect developmental processes, but to exploit their modularity for the design of custom living systems used in bioproduction, remediation, and regenerative medicine. Currently, our capacity to harness this potential is fundamentally limited by a lack of spatiotemporal control over gene expression in multicellular systems. While several synthetic genetic circuits for control of multicellular patterning have been reported, hierarchical induction of gene expression domains has received little attention from synthetic biologists, despite its fundamental role in biological self-organization. In this thesis, I introduce the first synthetic genetic system implementing population-based AND logic for programmed hierarchical patterning of bacterial populations of Escherichia coli, and address fundamental prerequisites for implementation of an analogous genetic circuit into the emergent multicellular plant model Marchantia polymorpha. In both model systems, I explore the use of bacteriophage T7 RNA polymerase as a gene expression engine to control synthetic patterning across populations of cells. In E. coli, I developed a ratiometric assay of bacteriophage T7 RNA polymerase activity, which I used to systematically characterize different intact and split enzyme variants. I utilized the best-performing variant to build a three-color patterning system responsive to two different homoserine lactones. I validated the AND gate-like behavior of this system both in cell suspension and in surface culture. Then, I used the synthetic circuit in a membrane-based spatial assay to demonstrate programmed hierarchical patterning of gene expression across bacterial populations. To prepare the adaption of bacteriophage T7 RNA polymerase-driven synthetic patterning from the prokaryote E. coli to the eukaryote M. polymorpha, I developed a toolbox of genetic elements for spatial gene expression control in the liverwort: I analyzed codon usage across the transcriptome of M. polymorpha, and used insights gained to design codon-optimized fluorescent reporters successfully expressed from its nuclear and chloroplast genomes. For targeting of bacteriophage T7 RNA polymerase to these cellular compartments, I functionally validated nuclear localization signals and chloroplast transit peptides. For spatiotemporal control of bacteriophage T7 RNA polymerase in M. polymorpha, I characterized spatially restricted and inducible promoters. For facilitated posttranscriptional processing of target transcripts, I functionally validated viral enhancer sequences in M. polymorpha. Taking advantage of this genetic toolbox, I introduced inducible nuclear-targeted bacteriophage T7 RNA polymerase into M. polymorpha. I showed implementation of the bacteriophage T7 RNA polymerase/PT7 expression system accompanied by hypermethylation of its target nuclear transgene. My observations suggest operation of efficient epigenetic gene silencing in M. polymorpha, and guide future efforts in chassis engineering of this multicellular plant model. Furthermore, my results encourage utilization of spatiotemporally controlled bacteriophage T7 RNA polymerase as a targeted silencing system for functional genomic studies and morphogenetic engineering in the liverwort. Taken together, the work presented enhances our capacity for spatiotemporal gene expression control in bacterial populations and plants, facilitating future efforts in synthetic morphogenesis for applications in synthetic biology and metabolic engineering.