The first aim of this master thesis was to summarize the information on injuries of ligamentous apparatus of an ankle, lateral instability of a talar joint, conservative and operative therapy of these injuries with the emphasis on physiotherapeutical procedures in the therapy of ankle sprains. The second aim of the master thesis was to introduce surface electromyography as a possibility to examine objectively the differences in the activation of foot and shank muscles with healthy people and people with chronic instability of an ankle in different postural situations. The third aim was to use the theoretical knowledge in practice with patients who have sprained their ankle and to make a subjective and objective assessment of the therapy effect which is in progress. Powered by TCPDF (www.tcpdf.org)

Despite tremendous progress in medicine, injuries of the adult central neural system remain without satisfactory solution. Regenerative medicine employs tissue engineering, cellular therapies, medical devices, gene therapy, or growth factors with the aim to bridge the lesion, re-establish lost connections and enhance endogenous repair in order to restore neural function. The aim of my thesis was to evaluate therapeutic potential of two approaches, transplantation of human mesenchymal stromal cells (hMSCs) and biological scaffolds derived from extracellular matrix (ECM) for neural regeneration, particularly in models of spinal cord injury (SCI). First, hMSCs from various sources - bone marrow (BM), adipose tissue (AT) and Wharton's jelly (WJ) - were isolated and characterized in vitro. All cell types met the minimal criteria for MSC phenotype and displayed similar properties in terms of their surface marker expression, differentiation potential, migratory capacity, and secretion of cytokines and growth factors. On the other hand, the cell yield from WJ and AT was significantly higher, and MSCs isolated from these tissues proliferated better than from BM. Therapeutic effect of intrathecal application of hWJ-MSCs was then evaluated in SCI compression model in rats. The effect of low (0.5 million) and...