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Animals deficient in complement components C1q, C4, C3, and CR1/2 have severely impaired antibody responses. C1q is primarily activated by antibody-antigen complexes. Antigen-specific IgM in complex with an antigen is able to enhance the antibody response against that antigen. This is dependent on the ability of IgM to activate complement. Naïve mice have very low amounts of specific antibodies and therefore it is surprising that classical pathway activation plays a role for primary antibody responses. It was hypothesized that natural IgM, present in naïve mice, would bind an antigen with enough affinity to activate C1q. To test this, a knock-in mouse strain, Cm13, with a point mutation in m heavy chain, making its IgM unable to activate complement was constructed. Surprisingly, the antibody responses in Cm13 were normal. Puzzled by the finding that the ability of IgM to activate complement was required only for some effects, the immunization protocol was changed to mimic an infectious scenario. With this regime, Cm13 mice had an impaired antibody response compared to wildtype (WT) mice. The antibody response in WT mice to these repeated low-dose immunizations was also enhanced. These observations suggest that IgM-mediated enhancement indeed plays a physiological role in initiation of early antibody responses. IgM-mediated enhancement cannot however compensate for the dependecy of T-cell help. Although IgM from WT mice enhanced the antibody response, the T-cell response was not enhanced. The connection between classical pathway activation and CR1/2 is thought to be generation of ligands for CR1/2. In mice, CR1/2 are expressed on B cells and follicular dendritic cells (FDC). Although CR1/2 are crucial for a normal antibody response, the molecular mechanism(s) are not understood. To investigate whether CR1/2 must be expressed on B-cells or FDC to generate a normal antibody response, chimeric mice between WT and CR1/2-deficient mice were constructed. The results show that CR1/2+ FDC were crucial for the generation of antibody responses. In the presence of CR1/2+ FDC, both CR1/2+ and CR1/2- B cells were equally good antibody producers. However, for an optimally enhanced antibody response against IgM-antigen complexes, both B cells and FDC needed to express CR1/2.

No description available.

No description available.

In modern society, individuals constantly pass judgments on their own body and physical competence as well as that of other people. All too often, the verdict is less favourable. For the person, these physical self-perceptions (PSP) may negatively affect global self-esteem, identity, and general mental well being. The overall aim of this thesis is to examine primarily the role that exercise, but also the roles that gender and culture, play in the formation of PSP. In Study I, using confirmatory factor analyses, strong support for the validity of a first-order, and a second-order hierarchical and multidimensional model of the Physical Self-Perception Profile (PSPP: Fox & Corbin, 1989) was found across three national samples (Great Britain, Sweden and Turkey) of university students. Cross-cultural differences were detected, with the British sample demonstrating higher latent means on all PSPP subdomains except for the physical condition subdomain (Condition), than the Swedish and Turkish samples. In Study II, a higher self-reported exercise frequency was associated with more positive PSP (in particular for Condition) and more importance attributed to PSP in Swedish university students. Males demonstrated higher overall PSPP-scores than females. In Study III, a true-experimental design with randomisation into an intervention and a control group was adopted. Strong support for the effects of an empowerment-based exercise intervention programme on PSP and social physique anxiety (SPA) over six months for adolescent girls was found. The relations of exercise, gender and culture with PSP, SPA and self-esteem are discussed from the standpoints of a variety of theoretical models (the EXSEM-model), and frameworks (self-presentation and objectification theory). The two theories of self-enhancement and skill-development are examined with regard to the direction of the exercise-physical self relationship and motivation for exercise. Arguments for the relevance of exercise and PSP for practitioners in promoting general mental well-being and preventing modern-day diseases are outlined.

Aim:  Optimize the usage of transactional emails, going beyond their communicative nature and combining it with marketing purposes. The project has been developed in collaboration with Incomedia, Italian software developer and vendor. Objective: Understand how Incomedia can exploit the benefits of transactional emails in a marketing perspective in order to increase the sales of its software. Limitation: The specificity of the topic, strictly related to Incomedia’s activities, products and consumers. Limits of time and variables tested with the A/B experiment. Theory/Methodology : It helped us to leverage the potential of transactional emails through the improvement of one particular element, the price discount offers. Due to the particularity of the software “medium-price” level, we have choose to do an A/B test experiment of the new transactional email by presenting the discount in two different ways: monetary and percentage terms. Result: The new transactional email, with the price discount, drove us to satisfactory results. The price discount expressed in percentage was better perceived and accepted by consumers; thanks to this, Incomedia during the experiment could highly increase its sales.

Business processes are fundamental to the operations of a company. Each product manufactured and every service provided is the result of a series of actions that constitute a business process. Business process management is an organizational principle that makes the processes of a company explicit and offers capabilities to implement procedures, control their execution, analyze their performance, and improve them. Therefore, business processes are documented as process models that capture these actions and their execution ordering, and make them accessible to stakeholders. As these models are an essential knowledge asset, they need to be managed effectively. In particular, the discovery and reuse of existing knowledge becomes challenging in the light of companies maintaining hundreds and thousands of process models. In practice, searching process models has been solved only superficially by means of free-text search of process names and their descriptions. Scientific contributions are limited in their scope, as they either present measures for process similarity or elaborate on query languages to search for particular aspects. However, they fall short in addressing efficient search, the presentation of search results, and the support to reuse discovered models. This thesis presents a novel search method, where a query is expressed by an exemplary business process model that describes the behavior of a possible answer. This method builds upon a formal framework that captures and compares the behavior of process models by the execution ordering of actions. The framework contributes a conceptual notion of behavioral distance that quantifies commonalities and differences of a pair of process models, and enables process model search. Based on behavioral distances, a set of measures is proposed that evaluate the quality of a particular search result to guide the user in assessing the returned matches. A projection of behavioral aspects to a process model enables highlighting relevant fragments that led to a match and facilitates its reuse. The thesis further elaborates on two search techniques that provide concrete behavioral distance functions as an instantiation of the formal framework. Querying enables search with a notion of behavioral inclusion with regard to the query. In contrast, similarity search obtains process models that are similar to a query, even if the query is not precisely matched. For both techniques, indexes are presented that enable efficient search. Methods to evaluate the quality and performance of process model search are introduced and applied to the techniques of this thesis. They show good results with regard to human assessment and scalability in a practical setting. / Geschäftsprozesse bilden die Grundlage eines jeden Unternehmens, da jedes Produkt und jede Dienstleistung das Ergebnis einer Reihe von Arbeitsschritten sind, deren Ablauf einen Geschäftsprozess darstellen. Das Geschäftsprozessmanagement rückt diese Prozesse ins Zentrum der Betrachtung und stellt Methoden bereit, um Prozesse umzusetzen, abzuwickeln und, basierend auf einer Auswertung ihrer Ausführung, zu verbessern. Zu diesem Zweck werden Geschäftsprozesse in Form von Prozessmodellen dokumentiert, welche die auszuführenden Arbeitsschritte und ihre Ausführungsbeziehungen erfassen und damit eine wesentliche Grundlage des Geschäftsprozessmanagements bilden. Um dieses Wissen verwerten zu können, muss es gut organisiert und leicht auffindbar sein – eine schwierige Aufgabe angesichts hunderter bzw. tausender Prozessmodelle, welche moderne Unternehmen unterhalten. In der Praxis haben sich bisher lediglich einfache Suchmethoden etabliert, zum Beispiel Freitextsuche in Prozessbeschreibungen. Wissenschaftliche Ansätze hingegen betrachten Ähnlichkeitsmaße und Anfragesprachen für Prozessmodelle, vernachlässigen dabei aber Maßnahmen zur effizienten Suche, sowie die verständliche Wiedergabe eines Suchergebnisses und Hilfestellungen für dessen Verwendung. Diese Dissertation stellt einen neuen Ansatz für die Prozessmodellsuche vor, wobei statt einer Anfragesprache Prozessmodelle zur Formulierung einer Anfrage verwendet werden, welche exemplarisch das Verhalten der gesuchten Prozesse beschreiben. Dieser Ansatz fußt auf einem formalen Framework, welches ein konzeptionelles Distanzmaß zur Bewertung gemeinsamen Verhaltens zweier Geschäftsprozesse definiert und die Grundlage zur Suche bildet. Darauf aufbauend werden Qualitätsmaße vorgestellt, die einem Benutzer bei der Bewertung von Suchergebnissen behilflich sind. Verhaltensausschnitte, die zur Aufnahme in das Suchergebnis geführt haben, können im Prozessmodell hervorgehoben werden. Die Arbeit führt zwei Suchtechniken ein, die konkrete Distanzmaße einsetzen, um Prozesse zu suchen, die das Verhalten einer Anfrage exakt enthalten (Querying), oder diesem in Bezug auf das Verhalten ähnlich sind (Similarity Search). Für beide Techniken werden Indexstrukturen vorgestellt, die effizientes Suchen ermöglichen. Abschließend werden allgemeine Methoden zur Evaluierung von Prozessmodellsuchansätzen vorgestellt, mit welchen die genannten Suchtechniken überprüft werden. Im Ergebnis zeigen diese eine hohe Qualität der Suchergebnisse hinsichtlich einer Vergleichsstudie mit Prozessexperten, sowie gute Skalierbarkeit für große Prozessmodellsammlungen.

Using a participatory ergonomic approach, this research explored if the self-identified needs and concerns of computer users are reflected in a typical occupational therapy office ergonomic educational presentation. While the study confirmed that generally the needs of computer users are met, the topics that the participants found to be most relevant were workstation layout and equipment adjustment. This study also revealed that knowledge transfer/translation is an important factor and that clinicians should consider involving clients at the initial development phase of client educational information. The study participants preferred ergonomic information to be communicated in a brief manner, emphasizing action-oriented information and avoiding medical references. They wanted client information to employ humour, colour and lots of “pizzazz.” The manner in which clinical information is communicated to clients is vital for effective client education.

This thesis investigated women’s self-presentation when seeking a romantic partner on an online dating site. Fifteen online dating profiles written by women, ages 25 to 29, seeking a male partner were collected and analyzed. Narrative analysis was used to study the profiles’ thematic content and structural form. From the thematic analysis, two stories emerged: the Single Woman Story and the Relationship Story. Within the Single Woman Story, there were four themes: Poetic and Philosophical, Idealistic, Independent, and Active. Within the Relationship Story, there were two main themes: Adventures of a New Life Stage, and Sharing a Life with Someone. From the structural analysis, a continuum was evident: at one end, women focused primarily on themselves (Who I Am) and at the other end, women primarily focused on a potential partner (Who You Are). The strengths and limitations of the study, along with implications of the results are discussed.

Biologically active 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) binds the vitamin D receptor (VDR) to exert its effect on target cells. VDR expression is found in a number of immune cells including professional antigen-presenting cells such as dendritic cells. It has been found that the actions of 1,25-(OH)2D3 on the immune system are mainly immunosuppressive. The cross-presentation pathway allows for exogenously derived antigens to be presented by pAPCs on MHC-I molecules to CD8+ T cells. CD8+ T cell activation results in the expansion of epitope-specific T cell populations that confer host protection. These epitopes can be organized into an immunodominance hierarchy. Previous work demonstrated that introducing LCMV-NP via the cross-priming pathway significantly alters the immunodominance hierarchy of a subsequent LCMV infection. Building upon these observations, our study assessed the effects of LCMV-NP cross priming in the presence of a single dose of 1,25-(OH)2D3. Treatment with 1,25-(OH)2D3 was found to have biological effects in our model system. In vitro pAPCs were demonstrated to up-regulate IL-10 and CYP24A1 mRNA, in addition to the transactivation of cellular VDR, as demonstrated by a relocalization to the nuclear region. Mice treated with 1,25-(OH)2D3 were found to produce up-regulated IL-10 and CYP24A1 transcripts. Expression of VDR was increased at both the transcript and protein level. Our results demonstrate that a single dose of 1,25-(OH)2D3 does not affect the cross-priming pathway in this system. Treatment with 1,25-(OH)2D3 did not influence the ability of differentiated pAPCs to phagocytose or cross-present exogenous antigen to epitope-specific CD8+ T cells. Furthermore, 1,25-(OH)2D3 did not alter cross-priming or the establishment of the LCMV immunodominance hierarchy in vivo. By confirming that 1,25-(OH)2D3 does not suppress cross-priming in our model, our study helps to expand the understanding of the immunomodulatory role of exogenous 1,25-(OH)2D3 on the outcome of virus infection. Collectively, our data supports the observation that the role of 1,25-(OH)2D3 in the immune system is not always associated with suppressive effects. / Thesis (Master, Microbiology & Immunology) -- Queen's University, 2011-08-29 14:53:18.766