Foram coletadas amostras de uma chapa grossa temperada, de aço microligado ao boro com 8 mm de espessura. O aço foi produzido em aciaria do tipo LD, laminado na forma de chapa grossa em laminador de cadeira única reversível e finalmente recebeu tratamento de têmpera em forno de tubos radiantes e máquina de têmpera com fluxo de água. Todas as etapas de produção tiveram os parâmetros de processo controlados para obtenção das melhores propriedades possíveis. As amostras foram retiradas da região situada a um quarto da largura da chapa e submetidas a diversos ciclos térmicos em aparelho de dilatometria. Com auxílio de técnicas de microscopia ótica, microscopia eletrônica de varredura e medidas de dureza, o aço pôde ser caracterizado e foi possível levantar as curvas de resfriamento contínuo (TRC). As microestruturas revelaram a presença de múltiplos microconstituintes, principalmente em resfriamentos a partir de resfriamentos 25°C/s, quando surgem elementos aciculares como ferrita acicular e bainita misturados com ferrita poligonal e perlita. Acima desta taxa de resfriamento o campo perlítico é suprimido e a partir de 45°C/s surge a martensita. A 100°C/s a microestrutura é dominada predominantemente por martensita. As amostras resfriadas nas 3 taxas mais severas também revelaram a presença de austenita retida e constituinte MA, detectados por meio de difração de raios-x e ataque com reagente de Klemm. / Samples were taken from a quenched plate with 8mm thickness and chemical composition consisting of carbon, manganese, silicon, aluminum, niobium, titanium and boron. The steel was produced in LD converter, rolled in a reversible single chair rolling mill and quenched in a radiating tube furnace and quenching machine with water flow. All production stages had the process parameters controlled to obtain the best possible properties. The samples were extracted from a position situated at one quarter of the plate´s width and submitted to various thermal cycles in dilatometry device. With the aid of optical microscopy, scanning electron microscopy and hardness measurements, the steel could be characterized and the continuous cooling transformation curves could (CCT) be plotted. The microstructures revealed the presence of multiple components, especially when cooling rates reach 25°C/s, where acicular elements, like acicular ferrite and bainite, are found along with polygonal ferrite and pearlite. Above this cooling rate the pearlite field is suppressed and starting at 45°C/s martensite was found. At 100°C/s the microstructure is mainly martensitic. The samples cooled at the 3 most severe rates also revealed the presence of retained austenite and MA constituent, which were detected by x-ray diffraction and attack using Klemm reagent.

This thesis presents an experimental study of the influence of an external field and alignment upon a colloid of a liquid crystal (octycyanobiphenyl denoted 8CB) and a silica gel of aerosil nano-particles. The first techniques used was an AC-calorimetry (alternating current heating) and the systems under investigation were firstly put under the influence of a magnetic field at John Hopkins University in Baltimore by professor Leheny's group. The experiments revealed changes in transition temperatures, nematic range and critical coefficient that could account for what we called a 'memory' of the above mentioned structures. The second technique, dielectric spectroscopy, was applied to the same very densities of mixture s mentioned in the first paragraph. The samples were applied in one procedure an increasingly higher alternating electric field. An overall increase of the capacitance of the sample was measured. The second experiment was to reproduce the application of the magnetic field from the AC-calorimetry experiment now with an electric field. In dielectric spectroscopy case, an increase in transition temperature after the application of the procedure was revealed.

Orientador: Gilberto de Magalhães Bento Golçaves / Banca: Carlos Elias da Silva Junior / Banca: Geraldo Luiz Palma / Resumo: Este trabalho avaliou as ocorrências mecânicas, macro e microestrutural em metal de solda de juntas de aço estrutural temperado e revenido, soldadas pelo processo a arco elétrico com proteção gasosa (GMAW) de 80%Ar-20%CO2, em modo convencional e pulsado, utilizando eletrodo de alma metálica ("metal cored"), designação AWS E70C-6M. As soldas foram realizadas em dois passes, um por lado de juntas preparadas com chanfro duplo "V"60º. Para uma mesma energia teórica de soldagem, comparativamente verificou-se aumentos nas eficiências de deposição e térmica do processo para a soldagem com arco pulsado, além de uma maior influência na diluição do metal de base e nas características geométricas dos cordões. Entretanto, foi constatada maior resistência mecânica para os metais de solda obtidos em modo convencional, atribuído à ocorrência de um refinamento microestrutural mais intenso nesta condição de soldagem. A expectativa desta contribuição é de que disponibilize maiores informações em metalurgia da soldagem para atender principalmente o segmento de fabricação de equipamentos e sistemas mecânicos aplicados a serviços abrasivos / Abstract: This study evaluated the mechanical, macro and microestrurtural events on metal weld joints of quenched and tempered structural steel, welded by eletric arc process with protective gas (GMAW) of 80% Ar-20% CO2 in conventional and pulsed mode using metal cored electrode, designation AWS E70C-6M. The welds were made in two passes, one on each side of joints prepared with 60º double "V" bevel. For a given theoretical energy welding, comaratively there was increases in the efficiencies of deposition and thermal for pulsed arc welding process, and a greater influence on the dilution of the base metal and the geometrical characteristics of the cords. However, was observed a higher mechanical strength for a weld metals obtained in conventional manner, attributed to the occurrence of an intense microstructural refinement in this welding condition. The expectation of this contribution is to make evailable more information on welding metallurgy to meet especially the segment of manufacturing equipment and mechanical systems applied to abrasive services / Mestre

Made available in DSpace on 2014-06-11T19:28:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-17Bitstream added on 2014-06-13T19:57:37Z : No. of bitstreams: 1 sarni_mij_me_bauru.pdf: 941928 bytes, checksum: 4ead015efe149db89a0c631bd5132b8b (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Este trabalho avaliou as ocorrências mecânicas, macro e microestrutural em metal de solda de juntas de aço estrutural temperado e revenido, soldadas pelo processo a arco elétrico com proteção gasosa (GMAW) de 80%Ar-20%CO2, em modo convencional e pulsado, utilizando eletrodo de alma metálica (metal cored), designação AWS E70C-6M. As soldas foram realizadas em dois passes, um por lado de juntas preparadas com chanfro duplo V60º. Para uma mesma energia teórica de soldagem, comparativamente verificou-se aumentos nas eficiências de deposição e térmica do processo para a soldagem com arco pulsado, além de uma maior influência na diluição do metal de base e nas características geométricas dos cordões. Entretanto, foi constatada maior resistência mecânica para os metais de solda obtidos em modo convencional, atribuído à ocorrência de um refinamento microestrutural mais intenso nesta condição de soldagem. A expectativa desta contribuição é de que disponibilize maiores informações em metalurgia da soldagem para atender principalmente o segmento de fabricação de equipamentos e sistemas mecânicos aplicados a serviços abrasivos / This study evaluated the mechanical, macro and microestrurtural events on metal weld joints of quenched and tempered structural steel, welded by eletric arc process with protective gas (GMAW) of 80% Ar-20% CO2 in conventional and pulsed mode using metal cored electrode, designation AWS E70C-6M. The welds were made in two passes, one on each side of joints prepared with 60º double V bevel. For a given theoretical energy welding, comaratively there was increases in the efficiencies of deposition and thermal for pulsed arc welding process, and a greater influence on the dilution of the base metal and the geometrical characteristics of the cords. However, was observed a higher mechanical strength for a weld metals obtained in conventional manner, attributed to the occurrence of an intense microstructural refinement in this welding condition. The expectation of this contribution is to make evailable more information on welding metallurgy to meet especially the segment of manufacturing equipment and mechanical systems applied to abrasive services

Foram coletadas amostras de uma chapa grossa temperada, de aço microligado ao boro com 8 mm de espessura. O aço foi produzido em aciaria do tipo LD, laminado na forma de chapa grossa em laminador de cadeira única reversível e finalmente recebeu tratamento de têmpera em forno de tubos radiantes e máquina de têmpera com fluxo de água. Todas as etapas de produção tiveram os parâmetros de processo controlados para obtenção das melhores propriedades possíveis. As amostras foram retiradas da região situada a um quarto da largura da chapa e submetidas a diversos ciclos térmicos em aparelho de dilatometria. Com auxílio de técnicas de microscopia ótica, microscopia eletrônica de varredura e medidas de dureza, o aço pôde ser caracterizado e foi possível levantar as curvas de resfriamento contínuo (TRC). As microestruturas revelaram a presença de múltiplos microconstituintes, principalmente em resfriamentos a partir de resfriamentos 25°C/s, quando surgem elementos aciculares como ferrita acicular e bainita misturados com ferrita poligonal e perlita. Acima desta taxa de resfriamento o campo perlítico é suprimido e a partir de 45°C/s surge a martensita. A 100°C/s a microestrutura é dominada predominantemente por martensita. As amostras resfriadas nas 3 taxas mais severas também revelaram a presença de austenita retida e constituinte MA, detectados por meio de difração de raios-x e ataque com reagente de Klemm. / Samples were taken from a quenched plate with 8mm thickness and chemical composition consisting of carbon, manganese, silicon, aluminum, niobium, titanium and boron. The steel was produced in LD converter, rolled in a reversible single chair rolling mill and quenched in a radiating tube furnace and quenching machine with water flow. All production stages had the process parameters controlled to obtain the best possible properties. The samples were extracted from a position situated at one quarter of the plate´s width and submitted to various thermal cycles in dilatometry device. With the aid of optical microscopy, scanning electron microscopy and hardness measurements, the steel could be characterized and the continuous cooling transformation curves could (CCT) be plotted. The microstructures revealed the presence of multiple components, especially when cooling rates reach 25°C/s, where acicular elements, like acicular ferrite and bainite, are found along with polygonal ferrite and pearlite. Above this cooling rate the pearlite field is suppressed and starting at 45°C/s martensite was found. At 100°C/s the microstructure is mainly martensitic. The samples cooled at the 3 most severe rates also revealed the presence of retained austenite and MA constituent, which were detected by x-ray diffraction and attack using Klemm reagent.

Glutamate carboxypeptidase II (GCPII) is a zinc-dependent carboxypeptidase with high expression levels in prostate carcinoma. As the enzyme represents a validated target for cancer therapy and imaging, the development of new GCPII-specific ligands is still a focus of an active academic and industrial research. However, existing assays to screen inhibitor libraries and determine inhibitor efficacy are suboptimal at best. This thesis is aimed at the development of small internally quenched probes that could be used for continuous measurement of the GCPII enzymatic activity. These probes are derived from natural GCPII substrates and consist of a fluorophore/quencher pair connected by a GCPII-hydrolysable linker. I first characterized biophysical properties of the probes and then determined kinetic parameters of their hydrolysis by GCPII. The optimized activity assay was then used to determine inhibition constants of several GCPII-specific inhibitors. Finally, complexes between the inactive enzyme and several probes were co-crystallized and one of the complexes refined and analyzed. Our data show that the probes are involved in non-covalent interactions with the same amino acid residues of the enzyme's active site as natural substrates. The developed assay could be optimized for high-throughput...

Proteinase-activated receptor-2 (PAR-2) is a member of a family of G-protein-coupled, seven-transmembrane domain receptors that are activated by proteolytic cleavage. The receptor is expressed in a number of different tissues and potential physiological activators identified thus far include trypsin and mast cell tryptase. Acrosin, a trypsin-like serine proteinase found in spermatozoa of all mammals, was found to cleave a model peptide fluorescent quenched substrate representing the cleavage site of PAR-2. This substrate was cleaved with kinetics similar to those of the known PAR-2 activators, trypsin and mast cell tryptase. Acrosin was also shown to induce significant intracellular calcium responses in Chinese hamster ovary cells stably expressing intact human PAR-2, most probably due to activation of the receptor. Immunohistochemical studies using PAR-2 specific antibodies indicated that the receptor is expressed by mouse oocytes, which suggests that acrosin may play additional role(s) in the fertilization process via the activation of PAR-2 on oocytes.

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