Made available in DSpace on 2014-10-09T12:36:05Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:59:23Z (GMT). No. of bitstreams: 1 04238.pdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

Made available in DSpace on 2014-10-09T12:24:41Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:05:02Z (GMT). No. of bitstreams: 1 01251.pdf: 1643812 bytes, checksum: 65192b877e878aea892352cedb15f5a6 (MD5) / Tese (Doutoramento) / IEA/T / Instituto de Biociencias, Universidade de Sao Paulo - IB/USP

Made available in DSpace on 2014-10-09T12:23:47Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:06:51Z (GMT). No. of bitstreams: 1 01287.pdf: 577057 bytes, checksum: 28c9a3861dd9f4082e899a67976d769e (MD5) / Dissertacao (Mestrado) / IEA/D / Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo - FMVZ/USP

Made available in DSpace on 2014-10-09T12:36:05Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:59:23Z (GMT). No. of bitstreams: 1 04238.pdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

Made available in DSpace on 2014-10-09T12:24:41Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:05:02Z (GMT). No. of bitstreams: 1 01251.pdf: 1643812 bytes, checksum: 65192b877e878aea892352cedb15f5a6 (MD5) / Tese (Doutoramento) / IEA/T / Instituto de Biociencias, Universidade de Sao Paulo - IB/USP

Made available in DSpace on 2014-10-09T12:23:47Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:06:51Z (GMT). No. of bitstreams: 1 01287.pdf: 577057 bytes, checksum: 28c9a3861dd9f4082e899a67976d769e (MD5) / Dissertacao (Mestrado) / IEA/D / Faculdade de Medicina Veterinaria e Zootecnia, Universidade de Sao Paulo - FMVZ/USP

to evaluate the GIP response to feeding and oral glucose tolerance tests. Initial evidence for the insulinotropic action of GIP was presented. Indicated also was an implication of GIP involvement in various pathological conditions. / Medicine, Faculty of / Cellular and Physiological Sciences, Department of / Graduate

A sensitive and reproducible double antibody radioimmunoassay technique, requiring 50ul of unknown serum or plasma per assay tube, is described for use with 125I and rabbit anti-rat LH serum. The assay system was applied to the study of LH secretion in rats under both normal and experimentally manipulated conditions. Particular attention was focussed upon comparison of circulating LH levels in conscious, unstressed animals with those in anaesthetized animals, with or without surgical stress. Thereafter, the effects of acoustic stimulation and of exogenous LRH administration were studied in conscious and anaesthetized animals. Urethane anaesthesia exerted a profound effect upon the LR-secretory response to exogenous LRH in male rats. Available evidence suggests that the blood sampling method, surgical stress and anaesthesia are each capable of significantly influencing LH secretion, thereby emphasizing the value of studies using conscious, unstressed animals. While a direct effect of urethane on the pituitary gland cannot be excluded, attention is drawn to the possible mediation of a urethane-sensitive inhibitory influence in the mechanism controlling LH secretion in the rat.

We have developed a solid-phase radioimmunoassay capable of detecting nanogram quantities of human IgA rheumatoid factor (RF) in biological fluids. Human IgM RF, IgG RF, IgG, IgA, IgM and whole serum did not significantly interfere with the IgA RF assay. Patients with sero-positive rheumatoid arthritis (RA) had significantly higher concentrations of IgA RF than sero-negative RA patients or healthy adult controls. Concentrations of IgA RF in paired sera and synovial fluids from sero-positive RA patients were comparable. Levels of IgA RF demonstrated a moderately good correlation with levels of IgM RF in sero-positive RA sera (r = 0.673). However, the ratio of IgA RF concentration to IgM RF concentration in sero-positive RA sera varied widely.

A microtiter, solid-phase, indirect radioimmunoassay (RIA) has been developed and evaluated as a method for detecting poliovirus in water samples. Antiserum to poliovirus Type I, LSc2ab, was prepared in rabbits, and high titer, high avidity antiserum to rabbit globulins were radio- actively labeled with 125I by a modification of the Bolton-Hunter and chloramine T methods to increasing specific activities. The immunoreactivity of the labeled antibodies was assessed. After the preparation and standardization of all components of the assay. The optimum assay conditions were determined. These conditions included the method of coating the microtiter wells with antigen, the time and temperature of incubation of each antiserum, the dilution of each antiserum, and means of reducing non-specific binding. The RIA was conducted with varying numbers of viral plaque forming units. In replicate experiments, average binding ratios of 784% were obtained when different numbers of virus were first reacted with antiserum. This technique shows increased sensitivity over the direct method. These results indicate that the use of RIA to detect viruses in water is indeed feasible.