Onset of puberty was observed in 12 bison bulls by the collection of semen at monthly intervals for 12 months beginning at 13 months of age. Onset of puberty was defined as the time in development when an ejaculate contained a minimum of 50x106 sperm showing at least 10% progressive motility. At each collection, data were recorded on body weight, semen quality, fecal testosterone concentration, and physical development. Semen was evaluated for gross motility, individual progressive motility, sperm morphology, sperm concentration and volume. From these data bison bulls attained onset of puberty at an average age of 16.5 months and an average body weight of 353 ± 52.8 kg. Age was the greatest determining factor for onset of puberty in this group of bulls. <p> Monthly abattoir collections of epididymal sperm (n=288) and testicular tissue (n=120) were evaluated to determine if bison bulls undergo seasonal changes in sperm production. Although epididymal sperm morphology did not give any indication of seasonal variation, the histological study of testicular tissue showed greater seminiferous tubule diameter (27.0 ± 4.3 ìm) during the breeding months (July, August and September) than during any other seasons. Semen collected at 4 different occasions during the year (June, November, January, and April) from live mature breeding bulls (n=21) was used to verify data collected from abattoir samples. Semen from mature bulls showed a significantly greater proportion of normal sperm in June than in November (73.8 ± 9.1%; 44.1 ± 24.3%), respectively. There was little improvement in sperm morphology at the January sampling but in April morphology improved to a level close to that observed in June. Fecal testosterone concentrations were highest in June (128.6 ± 67.4 ng/g) and lowest in April (48.5 ± 33.3 ng/g). Although there was no clear seasonal trend in sperm morphology from bulls sampled at the abattoir, mature bulls showed slight seasonal variations in semen quality.

Onset of puberty was observed in 12 bison bulls by the collection of semen at monthly intervals for 12 months beginning at 13 months of age. Onset of puberty was defined as the time in development when an ejaculate contained a minimum of 50x106 sperm showing at least 10% progressive motility. At each collection, data were recorded on body weight, semen quality, fecal testosterone concentration, and physical development. Semen was evaluated for gross motility, individual progressive motility, sperm morphology, sperm concentration and volume. From these data bison bulls attained onset of puberty at an average age of 16.5 months and an average body weight of 353 ± 52.8 kg. Age was the greatest determining factor for onset of puberty in this group of bulls. <p> Monthly abattoir collections of epididymal sperm (n=288) and testicular tissue (n=120) were evaluated to determine if bison bulls undergo seasonal changes in sperm production. Although epididymal sperm morphology did not give any indication of seasonal variation, the histological study of testicular tissue showed greater seminiferous tubule diameter (27.0 ± 4.3 ìm) during the breeding months (July, August and September) than during any other seasons. Semen collected at 4 different occasions during the year (June, November, January, and April) from live mature breeding bulls (n=21) was used to verify data collected from abattoir samples. Semen from mature bulls showed a significantly greater proportion of normal sperm in June than in November (73.8 ± 9.1%; 44.1 ± 24.3%), respectively. There was little improvement in sperm morphology at the January sampling but in April morphology improved to a level close to that observed in June. Fecal testosterone concentrations were highest in June (128.6 ± 67.4 ng/g) and lowest in April (48.5 ± 33.3 ng/g). Although there was no clear seasonal trend in sperm morphology from bulls sampled at the abattoir, mature bulls showed slight seasonal variations in semen quality.

Serum lipase activity has traditionally been used for diagnosis of pancreatitis in human beings and dogs. However, serum lipase activity is not specific for exocrine pancreatic function and many cell types other than pancreatic acinar cells also synthesize lipases. Recently, an immunoassay for the measurement of canine pancreatic lipase immunoreactivity has been developed and validated. This assay has shown to be specific for exocrine pancreatic function and sensitive for the diagnosis of canine pancreatitis. The objectives of this project were to purify feline pancreatic lipase (fPL), have antibodies against fPL (anti-fPL antibodies) produced, and develop a radioimmunoassay (RIA) for the diagnosis of feline pancreatitis. Pancreatic lipase was purified from feline pancreatic tissue by delipidation, anion-exchange chromatography, size-exclusion chromatography, and cation-exchange chromatography. Antiserum against fPL was raised in rabbits. Tracer was produced by iodination (125I) of fPL using the chloramine T method. An RIA was established and validated by determination of sensitivity, dilutional parallelism, spiking recovery, intraassay variability, and inter-assay variability. A control range for fPLI in cat serum was established from 30 clinically healthy cats using the central 95th percentile. The sensitivity of the fPLI assay was 1.2 &#956;g/L. Observed to expected ratios for serial dilutions ranged from 58.0 to 164.3% for 4 different serum samples at dilutions of 1 in 2, 1 in 4, and 1 in 8. Observed to expected ratios for spiking recovery ranged from 76.0 to 156.5% for 4 different serum samples and 6 different spiking concentrations. Coefficients of variation for intra-assay variability for 4 different serum samples were 10.1, 4.5, 2.2, and 3.9%. Coefficients of variation for inter-assay variability for 4 different serum samples were 24.4, 15.8, 16.6, and 21.3%. The control range for serum fPLI concentration was established as 1.2 to 3.8 &#956;g/L. All of the objectives outlined above were successfully met, leading to the development of an RIA for the measurement of fPLI in cat serum. The RIA for fPLI described here is sufficiently accurate and precise, but has a limited linearity and reproducibility in the lower and higher end of the working range.

Marburg, Universität, Thesis (doctoral), 2002.

No description available.

Lic-avh. (sammanfattning) Stockholm : Karolinska institutet, 2006. / Härtill 3 uppsatser.

Thesis (M.Sc. (Anatomy))--Mahidol University, 1982.

Universiẗat, Diss., 2004--München.

Tierärztl. Hochsch., Diss., 2004--Hannover.

Made available in DSpace on 2014-06-11T19:30:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-01-24Bitstream added on 2014-06-13T19:00:39Z : No. of bitstreams: 1 reis_acg_dr_jabo.pdf: 749766 bytes, checksum: 91288058e5c8e2a0b2b59a94f7d36cb3 (MD5) / Considerando-se a importância da paca, roedor histricomorfo componente da fauna silvestre brasileira, diante de seu potencial zootécnico e ainda da possibilidade de tornar-se uma nova opção de animal experimental e sopesando-se também o valor da aplicação de técnicas não invasivas na para elucidar fatores reprodutivos, visa-se com esta pesquisa, neste roedor, caracterizar as flutuações hormonais de progestinas e estrógenos fecais durante o ciclo estral e período gestacional, validar um método não invasivo para diagnóstico da gestação, bem como avaliar o uso de miçangas como forma de marcar e individualizar as amostras fecais. Nove fêmeas adultas, não nulíparas, diagnosticadas não prenhes mediante exame ultrassonográfico, tiveram suas fezes marcadas, por meio da ingestão de miçangas, e colhidas diariamente durante 43 dias consecutivos; após este período, machos foram adicionados aos grupos das fêmeas que tiveram as fezes colhidas, apenas uma vez por semana, até o parto. Os valores de progestágenos fecais observados durante o ciclo foram de 0,37 a 7,9 ng/g de fezes secas e de estrógenos fecais de 5,08 a 37,72 ng/g de fezes secas e não foi possível observar flutuações de progestágenos que caracterizassem fase lútea e que fosse compatível com picos de estradiol entre elevações de progestágenos. Durante a gestação os valores de progestágenos oscilaram de 1,33 a 6,42 ng/g de fezes secas; os valores de estrógenos fecais variaram de 8,97 a 1964 ng/g de fezes secas com acentuado aumento no segundo terço de gestação. Um diagnóstico de gestação pode ser confirmado com observação de níveis de estrógenos fecais acima de 41,06 ng/g de fezes por duas semanas consecutivas. O marcador plástico é item seguro e sem contraindicações na marcação e individualização das fezes de paca / Considering the importance of Cuniculus paca, a hystricomorph rodent component of Brazilian wildlife, its livestock potential besides the possibility that it might become a new option for animal experimentation and also the value of non invasive techniques to elucidation of reproductive parameters, the aim of this study was to characterize, for this rodent, hormonal fluctuations of fecal progestins and estrogens during estrous cycle and pregnancy, validate a non-invasive method for pregnancy diagnosis and evaluate the use of plastic beads as a fecal marker. Nine adult females, non nulliparous, non-pregnancy diagnosed by ultrasound, had their feaces marked, through the ingestion of beads, and collected daily for 43 days, after this period, males were added to female groups and the feaces were collected only once a week until parturition. The values of fecal progestagens observed throughout the cycle were 0.37 to 7.9 ng/g of dry feces and fecal estrogens were 5.08 to 37.72 ng/g dry feces; no progestins fluctuation that characterize luteal phase compatible with estradiol peak was observed. During pregnancy, progesterone values ranged from 1.33 to 6.42 ng/g dry feces, fecal estrogen values vary from 8.97 to 1964 ng/g dry feces with a sharp rise in the second third of gestation. A pregnancy diagnosis can be confirmed by observation of fecal estrogen levels up to 41.06 ng/g of feces for two consecutive weeks. The plastic marker was found to be safe and effective at yielding readily identifiable stools in pacas