Diss.--Philosophische Fakultät--Universität Potsdam, 2001. / Bibliogr. p. [245]-263. Index.

Diss.--Philosophische Fakultät--Bonn--Rheinische Friedrich-Wilhelms-Universität, 2000. / Bibliogr. p. 529-570. Index.

Diss.--Fakultät für Geschichte, Kunst- und Orientwissenschaften--Universität Leipzig, 1999. / Bibliogr. p. 429-466. Index.

Texte remanié de: Dissertation--Berlin--Humboldt-Universität, 2001. / Bibliogr. p. [271]-280. Index.

Diss.--Bonn, 2002. / Bibliogr. p. 460-488.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

Diss.--Universität Potsdam, 1995. / Bibliogr. p. 405-429. Index.

Diss.--Philosophische Fakultät--Kiel--Christian-Albrechts-Universität, 1991.

Orientador: Maria José Soares Mendes Giannini / Banca: Clarice Queico Fujimura Leite / Banca: Celia Maria de Almeida Soares / Banca: Paulo Inácio da Costa / Banca: Mário Hiroyuki Hirata / Resumo: As dermatofitoses são processos infecciosos de pele, pêlo e unhas muito comuns no mundo inteiro. Trichophyton rubrum é o dermatófito mais freqüentemente isolado em lesões superficiais de pele e unha. Estudos envolvendo este patógeno são cada vez mais importantes devido ao aparecimento de linhagens resistentes aos medicamentos antifúngicos disponíveis no mercado e ao comportamento invasivo deste agente em pacientes com o sistema imune comprometido. Estes fatos e poucos estudos levam à necessidade de se ampliar o conhecimento sobre a biologia deste agente. Visando colaborar nesse sentido, o presente trabalho teve dois objetivos centrais: 1) Realizar a tipagem molecular de isolados clínicos de T. rubrum com e sem relação epidemiológica por RAPD (Amplificação Randômica de DNA Polimórfico) com duas seqüências randômicas diferentes (denominadas de 1 e 6), bem como a análise dos elementos repetitivos (TRS-1 e TRS-2) do espaço não transcrito (NTS) do DNA ribossomal (DNAr) e 2) Identificar transcritos envolvidos na interação deste patógeno com fonte humana de queratina, através do RDA (Análise de Diferença Representacional). A aplicação do RDA permitiu pela primeira vez a identificação de alguns transcritos expressos, provavelmente relacionados à patogênese deste microrganismo. / Abstract: Dermatophytosis is common infection process which occurs in skin, hair and nails and Trichophyton rubrum is the most frequently isolated dermatophyte. Studies on this pathogen are becoming increasingly important because of frequent reports on resistant strains to antifungal drugs commercially available and the invasive behavior of these agents in immunocompromised patients. These facts, associated with few studies with this agent, indicate the need to expand the information about the fungal biology. As a contribution to this goal, the present study had two central objectives: 1) To compare different methodologies for molecular typing of clinical isolates of T. rubrum epidemiologically related and unrelated for RAPD (Randomly Amplified Polymorphic DNA) with two different random primers (denominated of 1 and 6); as well as the analysis of the repetitive elements (TRS-1 and TRS-2) of the space non-transcribed (NTS) of the ribosomal DNA (DNAr) and 2) To identify transcripts involved in the interaction of this pathogen with human keratin by RDA (Representational Difference Analysis). The application of the RDA allowed for the first time the identification of expressed transcripts during the microorganism proliferation that could be related to the T. rubrum virulence. / Doutor

Rocky desertification (RD) is a process of land degradation that often results in extensive soil erosion, bedrock exposure and considerable decrease of land productivity. The spatio-temporal evolution of RD not only reflects regional ecological environmental changes but also directly impacts regional economic and social development. The study area, Hechi, is a typical karst peak cluster depression area in southwest China. Remote sensing, geographic information systems (GIS) and statistical techniques were employed to examine the evolution, including the identification of driving forces, of karst RD in the Northwestern Guangxi. The results indicate that RD became most apparent between 1990 and 2005 when areas of various types of RD increased. Within the karst RD landscape, slight RD was identified as the matrix of the landscape while potential RD had the largest patch sizes. Extremely strong RD, with the simplest shape, was the most influenced by human activities. Overall the landscape evolved from fragmented to agglomerate within the 15-year timeframe. Land condition changes were categorized as five types; desertified, recovered, unchanged, worsened, and alleviated land. The largest turnover within the RD landscape was between slight and moderate RD. With regards to the driving forces all RD had been increasingly influenced by human activities (i. e., the stronger the RD, the stronger the intensity of human disturbances). Dominant impact factors of the RD landscape had shifted from town influence and bare rock land in 1990 to bare rock and grassland in 2005. Moreover, the impacts of stony soil, mountainous proportion and river density on RD increased over time, while that of others decreased. The significant factors included human activities, land use, soil types, environmental geology, and topography. However, only anthropogenic factors (human activities and land use) were reported as leading factors whereas the others acted simply as constraining factors.