Maintaining replication fork integrity is vital to preserve genomic stability and avoid cancer. Physical DNA damage and altered nucleotide or protein pools represent replication obstacles, generating replicative stress. Numerous cellular responses have evolved to ensure faithful DNA replication despite such challenges. Understanding those responses is essential to understand and prevent or treat replication-associated diseases, such as cancer. Re-priming is a mechanism to allow resumption of DNA synthesis past a fork-stalling lesion. This was recently suggested in yeast and explains the formation of gaps during DNA replication on damaged DNA. Using a combination of assays, we indicate the existence of re-priming also in human cells following UV irradiation. The gap left behind a re-primed fork must be stabilised to avoid replication-associated collapse. Our results show that the checkpoint signalling protein CHK1 is dispensable for stabilisation of replication forks after UV irradiation, despite its role in replication fork progression on UV-damaged DNA. It is not known what proteins are necessary for collapse of an unsealed gap or a stalled fork. We exclude one, previously suggested, endonuclease from this mechanism in UV-irradiated human fibroblasts. We also show that focus formation of repair protein RAD51 is not necessarily associated with cellular sensitivity to agents inducing replicative stress, in rad51d CHO mutant cells. Multiple factors are required for replication fork stability, also under unperturbed conditions. We identify the histone methyltransferase SET8 as an important player in the maintenance of replication fork stability. SET8 is required for replication fork progression, and depletion of SET8 led to the formation of replication-associated DNA damage. In summary, our results increase the knowledge about mechanisms and signalling at replication forks in unperturbed cells and after induction of replicative stress. / At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: Submitted. Paper 2: Submitted. Paper 3: Manuscript. Paper 5: Submitted.

In the context of democratisation in the early 1990s, the governments in Central and East Europe (CEE) had to decide how to deal with property that had been confiscated under state socialism. Nationalised housing and collectivised land were to a varying extent returned to former owners and their heirs by means of restitution, as well as being distributed to other citizens who were in possession of the users’ rights to such properties. This thesis examines the spatial impacts, in terms of ownership patterns, of the way the redistribution of nationalised housing and collectivised land has been dealt with politically and at the local level in post-socialist Romania. It also locates the Romanian property reforms in relation to those of the rest of CEE. The impact of political directives on the property redistribution is analysed in relation to both structural influences, such as democratisation and antecedent property regimes, and implementation patterns in varied place-contexts. The thesis demonstrates that restitution was stifled due to disagreements between leftist and rightist political blocs, with the latter arguing for restitution whilst their opponents wrote the first restitution laws. A re-privatisation law allowed for the public sale of nationalised housing to tenants and thereby blocked the implementation of a restitution law, thus constituting a dilemma for constitutional democracy. In liberal place-contexts in West Romania, these obstacles to housing restitution were in part avoided. By contrast, land restitution was most widespread in the east, a stronghold of the left. This was because the legislation gives priority to restitution in areas of this kind, where smaller land-holdings dominated prior to 1945. The left-wing government pursued an electoral strategy of distributing small properties to a large number of citizens, and to current users in particular. This resulted in a fragmentation of historical property.

Despite several investigations and interventions aimed at decreasing the time from symptom onset to medical care seeking in acute myocardial infarction patients, the delay time is still too long for best treatment outcomes. In this thesis, investigations aimed at improving our understanding of the factors influencing delay time are evaluated, as well as attitudes to medical care seeking in patients, relatives and the general public. Additionally, an evaluation was performed to examine whether health-related quality of life had any influence on delay time and re-admissions. Participating patients, relatives and representatives of the general public were generally knowledgeable about acute myocardial infarction (AMI) and its symptomatology. The majority of participants knew about the importance of receiving fast treatment when an AMI occurs. Despite people’s knowledge, several patients and relatives felt uncertain of symptom origin and how to act at symptom onset. Patients commonly consulted an additional person when symptoms did not disappear. However, people appeared to act more appropriately if someone else had chest pain compared to self-experienced symptoms. In patients who had suffered from more than one AMI, poor total health status increased the risk of delaying for more than two hours, but no independent association was found between total health status and re-admissions within the first year post-AMI.

Shared Services (SS) involves the convergence and streamlining of an organisation’s functions to ensure timely service delivery as effectively and efficiently as possible. As a management structure designed to promote value generation, cost savings and improved service delivery by leveraging on economies of scale, the idea of SS is driven by cost reduction and improvements in quality levels of service and efficiency. Current conventional wisdom is that the potential for SS is increasing due to the increasing costs of changing systems and business requirements for organisations and in implementing and running information systems. In addition, due to commoditisation of large information systems such as enterprise systems, many common, supporting functions across organisations are becoming more similar than not, leading to an increasing overlap in processes and fuelling the notion that it is possible for organisations to derive benefits from collaborating and sharing their common services through an inter-organisational shared services (IOSS) arrangement. While there is some research on traditional SS, very little research has been done on IOSS. In particular, it is unclear what are the potential drivers and inhibitors of IOSS. As the concepts of IOSS and SS are closely related to that of Outsourcing, and their distinction is sometimes blurred, this research has the first objective of seeking a clear conceptual understanding of the differences between SS and Outsourcing (in motivators, arrangements, benefits, disadvantages, etc) and based on this conceptual understanding, the second objective of this research is to develop a decision model (Shared Services Potential model) which would aid organisations in deciding which arrangement would be more appropriate for them to adopt in pursuit of process improvements for their operations. As the context of the study is on universities in higher education sharing administrative services common to or across them and with the assumption that such services were homogenous in nature, this thesis also reports on a case study. The case study involved face to face interviews from representatives of an Australian university to explore the potential for IOSS. Our key findings suggest that it is possible for universities to share services common across them as most of them were currently using the same systems although independently.

Disability advocates in the twenty-first century have frowned upon the practice of institutionalization in disability services, yet many people with intellectual disability continue to be institutionalized in other settings such as correctional facilities. The prison system is a difficult environment for people with intellectual disability to negotiate, and they may find themselves victimized, segregated and isolated with very few resources to survive the experience. Incarceration may present a temporary solution to preventing anti-social behaviour in society. However, for offenders with intellectual disability, it often fails to address their criminal behaviour or the social context from which the behaviour emanates. Policy and practice in the disability service sector needs to develop further awareness of the needs of people with intellectual disability who come into contact with the criminal justice system as offenders. Similarly, correctional systems need to expand their knowledge base on the habilitative and rehabilitative needs of prisoners with intellectual disability in order to better address the issues that may arise from their incarceration. This exploratory qualitative study gathers the life stories of ten ex-prisoners from Queensland correctional facilities who have been labelled as having an intellectual disability by the service systems they have accessed. It utilizes an interpretive, social constructionist framework to understand people’s experiences. Life stories were gathered from in-depth interviews with participants over a prolonged period of time and supplemented by contextual information provided by six practitioners from disability, mental health and ex-prisoner services. The stories of three participants with intellectual disability were analysed through the holistic lens of a narrative approach and all ten stories were analysed thematically, providing an aggregate picture of all participants’ experiences. The findings of this thesis indicate that participants in this study had personal needs to belong, to feel competent and for others to see their criminal activity as an unfortunate but very human response to difficult circumstances. These needs were not always met within the prison environment and many participants struggled to feel safe in such a context. There were many pre-prison characteristics of participants that influenced their adaptation to prison, and they were also subjected to a series of degradations such as enforced isolation, frequent strip-searching, verbal and physical assault. However, these factors were not always constructed as negative or significant experiences by participants, and were often counterbalanced by perceived benefits to prison life such as friendship, food and reprieve from community living. These constructions of their experience highlight the vulnerability of this group within the prison environment and the failure of 2 the system and broader society to address core issues for people when they returned to the community. Significant disparities were also found between the philosophies of disability service support and the correctional enterprise. This study has indicated the urgent need for cross-agency collaboration in addressing the needs of people with intellectual disability. The thesis makes a contribution to both doing research with people with intellectual disability and to understanding their experiences of incarceration. The voices of people with intellectual disability have long been overlooked in research because it was assumed they could not express their views or because researchers did not have appropriate research approaches. It is only recently that some writers have captured the viewpoints of people with intellectual disability in academic discourse, but there has been little work done with prisoners and ex-prisoners with intellectual disability. Researching the experience of imprisonment with people with intellectual disabilities also presented unique ethical and methodological challenges. This thesis covers ethical issues such as informed consent, incriminating disclosure, self-determination and veracity and bias in life story research. It also develops knowledge in this area regarding recruitment, communication practices, and dissemination of findings. Overall, the study provides a nuanced account of life inside prison for people with intellectual disability. It makes a valuable contribution to the field of inclusive research with people with impaired capacity and to criminological research in this area.

Over the 1980s secondary schools and Technical and Further Education (TAFE) colleges in South Australia had increasing numbers of adults returning to formal secondary education mainly in search of better jobs or to qualify for tertiary studies. The teaching of such students required an appreciation of the difficulties they faced with the competing demands of family and work, and the anxieties they had in meeting the requirements of formalised study. In 1989 the South Australian Government made a policy decision, to transfer all the year 11 and 12 classes which TAFE colleges had specially established for adult students to the public secondary school system. Funds were allocated for the establishment of a secondary school system wide structure of nine Adult Re-entry sites, eight of which are still successfully operating within the Department of Education and Children’s Services. Whilst adult educational sites existed elsewhere, no other Australian state had a comparable systemic secondary school structure designed for adults returning to study. As a teacher of adult re-entry students, I sought to investigate the historical factors behind the policy decision to establish of adult re-entry sites within the secondary school system, to research the development of adult programs at a particular site and to study the experiences of adult students, analysing in particular why they returned to formal studies. Overseas studies indicated that to understand the personal worlds of adult students two sets of factors needed to be taken into account. External social and cultural factors influenced their current situation and their life experiences. Internal psychological factors helped to determine how they responded to the new demands of study. Four different educational responses to adult students could be identified. Direct and structured teaching could be seen in many vocational training models. Programs based on andrological principles put the emphasis on the individual’s self – directed learning. A third approach was focussed on critical pedagogy which sought to change society. There was also evidence of a holistic approach, which was centred on the adult learners but provided sympathetic educators to support their learning. The researcher’s underlying perspective in carrying out this study was that associated with Weber’s social action theory, because of the way it enabled emphasis to be placed on the actions of individuals and their interpretations of their actions in their social and cultural contexts. In this research portfolio the individuals concerned were politicians and educational administrators making policy decisions (Part 1); teachers developing appropriate programs (Part 2); and adult students deciding to return to studies and participating in adult programs (Part 3). Part 1 of Portfolio Two main sources of data were used to investigate the reasons for the 1989 policy decision to establish a system of adult re-entry colleges and schools within the Education Department of South Australia. The first was the official Hansard record of debates in the South Australian Parliament during the late 1980s and early 1990s. The second was a series of interviews with the Minister of Education involved and key officials in the Departments of Education and TAFE. The debates were focussed on the ideal educational location for adult re-entry students. Arguments based on the perspective of social justice and the special needs of adult students led to the establishment of a formal network of sites and programs to cater for the needs of adults within the secondary school sector. Despite the closure of one site in 1996 and funding readjustments in 1998, by 2005 adult re-entry colleges and schools had a well established role in South Australian public education system. Part 2 of Portfolio The investigation into the development of adult re-entry programs on one site was based on documents available in the Research School chosen – annual statistics, reports, curriculum, administration and journals. In addition, key members of staff involved in the adult program were interviewed. There was evidence of the way the adult program had changed over the period 1990 - 2005 in response to changing demography in the surrounding area and to changing needs and interests of those returning to study, as well as satisfying Departmental requirements. In recent years there has been a trend for more students to study part – time and to seek vocational rather than pre – university education. The provision of appropriate courses, resources and support was regarded by staff as important in the ongoing success of the adult re-entry program. Part 3 of Portfolio The investigation of adult students’ motivations and experiences in returning to study was based on the memoirs and personal statements of 40 adult re-entry students from the Research School. Their comments provided a unique understanding of the diverse personal worlds of adult re-entry students, their expectations, goals and aspirations, their difficulties and problems and their learning experiences. The formation of adult campuses in the secondary sector in South Australia was influenced by both pragmatic factors and by principles of social justice which sought to promote educational opportunities and offer those who had left school without recognised qualifications a second chance. Adult re-entry sites have continued to provide for the needs of adult learners in the communities they serve. They have made an important contribution both to the individual’s right to life – long learning and to society’s need for skilled workers and well educated professionals. During the twenty first century adult sites in South Australia within the Department of Education and Children’s Services have faced two challenges. The learning interests of adult students have changed, with more looking to the acquisition of technical and vocational skills. Furthermore, in order to remain viable, adult sites have had to maintain a high profile in relation to innovative policy development, student numbers, funding and resource allocation. Their successes have been due in large measure to their recognition that adult re-entry students were not big kids, but required specific educational structure and programs catering for their diverse learning needs. / http://proxy.library.adelaide.edu.au/login?url= http://library.adelaide.edu.au/cgi-bin/Pwebrecon.cgi?BBID=1415385 / Thesis (D.Ed.) -- University of Adelaide, School of Education, 2009

Thesis--Universität Göttingen, 2001. / Includes bibliographical references (p. 380-429) and indexes.

Thesis--Universität Göttingen, 2001. / Includes bibliographical references (p. 380-429) and indexes.

Zugl.: Frankfurt (Main), Hochsch. für Musik und Darstellende Kunst, Diss., 2005

Made available in DSpace on 2014-06-11T19:23:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-11-24Bitstream added on 2014-06-13T20:50:29Z : No. of bitstreams: 1 santos_vr_me_arafcf.pdf: 518686 bytes, checksum: 1d1116ed450de58c7b14e2cb0bbfce4b (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Aguardente de cana é a segunda bebida alcoólica mais consumida no Brasil, estimando-se sua produção em cerca de 1,2 bilhão de litros/ano. Em 2009 foram exportados 10,83 milhões de litros gerando uma receita de US$ 15,58 milhões. Dentre os principais mercados de destino estão Alemanha, Estados Unidos, Portugal e França (ALVES, 2009). Diante das novas exigências do mercado, os produtores começam a se preocupar em agregar maior valor à bebida, investindo em novas tecnologias e aperfeiçoamento de pessoal, no controle de qualidade e oferecendo embalagens mais sofisticadas, com o intuito de inserir a cachaça nos melhores pontos de consumo do país, ao lado de outras bebidas destiladas já consagradas no país e no mundo. A adição de açúcar tem sido utilizada como uma forma de padronizar o sabor de algumas bebidas destiladas, visando reduzir o sabor do etanol e eventuais defeitos sensoriais (CARDELLO e FARIA, 2000). O objetivo deste estudo foi avaliar o efeito da adição de açúcar na qualidade sensorial da cachaça, comparando-se amostras obtidas tradicionalmente e redestiladas contendo, diferentes concentrações de açúcar. As amostras foram obtidas a partir de um mesmo lote de aguardente tradicional considerada de baixa aceitação com graduação alcoólica de 47%. Inicialmente foram obtidas sete amostras, contendo concentrações crescentes de açúcar, que foram então diluídas para 40ºGL. Posteriormente após redestilação do lote acima referido foram obtidas amostras, contento também concentrações crescentes de açúcar e também diluídas para 40º GL. As 14 amostras de aguardente, obtidas conforme acima descrito, foram então submetidas a testes de aceitação em relação aos atributos aroma, sabor, doçura e impressão global, e atitude de compra. Os resultados demonstraram que a prática de adicionar açúcar na cachaça... / Sugarcane liquor is the second most consumed beverage in Brazil, with estimated production at about 1.2 billion liters/year. In 2009 exports reached 10.83 million liters generating revenues of US$ 15.58 million. Among the main destination markets are Germany, United States, Portugal and France. 7 The addition of sugar has been used as a way to standardize the flavor of some liquor, to reduce the taste of ethanol and other sensory defects. 3 The purpose of this study was to evaluate the effect of adding different concentrations of sugar in the sensory quality of cachaça, comparing samples obtained by the traditional way and by re-distillation. From the same bach of “cachaça” obtained traditionally and considered of low acceptance, seven samples were added of increasing concentrations of sugar, end then diluted to 40 GL. Subsequently, a volume of the same batch was redistilled in order to obtain more seven samples, also having increased concentrations of sugar and also diluted to 40 GL. These samples were then tested for acceptance in relation to the attributes aroma, flavor, sweetness, overall impression and purchase attitude. The results showed that the practice of adding sugar undoubtedly influences positively the final product acceptance, minimizing sensory defects related to inadequate techniques of production, as well showed that the re-distillation process may also improve the acceptance of the Brazilian cachaça