Global ETD Search

1	Dynamic Regulation of Slit/Robo Signaling Wang, Heng Rui 27 November 2012 (has links) The Slit family (Slit1-3) of secreted glycoproteins and their cognate Roundabout family (Robo1-4) of transmembrane receptors provide important repulsive signals to guide cell migration during development and postnatal life. The dynamic regulation of Slit/Robo signaling is poorly understood in vertebrates. In this study, we identified a novel role for endocytosis in regulating Slit2 /Robo1 expression. Using heterologous expression systems, Slit2 was found be endocytosed in a Robo1-dependent manner and subsequently degraded in the lysosome, while Robo1 was found to be primarily recycled. An AP-2 consensus binding site, which mediates clathrin-dependent endocytosis, was identified in the Robo1 cytoplasmic tail and found to be required for Slit2 down-regulation and Slit2-induced endocytosis of Robo1. Preliminary data suggests that Slit2-induced endocytosis of Robo1 may be required for downstream signaling. These findings have important implications for how Slit/Robo signaling may be dynamically regulated during cell migration. Receptor-mediated endocytosis Slit Robo Cell migration 0379 0307
2	Dynamic Regulation of Slit/Robo Signaling Wang, Heng Rui 27 November 2012 (has links) The Slit family (Slit1-3) of secreted glycoproteins and their cognate Roundabout family (Robo1-4) of transmembrane receptors provide important repulsive signals to guide cell migration during development and postnatal life. The dynamic regulation of Slit/Robo signaling is poorly understood in vertebrates. In this study, we identified a novel role for endocytosis in regulating Slit2 /Robo1 expression. Using heterologous expression systems, Slit2 was found be endocytosed in a Robo1-dependent manner and subsequently degraded in the lysosome, while Robo1 was found to be primarily recycled. An AP-2 consensus binding site, which mediates clathrin-dependent endocytosis, was identified in the Robo1 cytoplasmic tail and found to be required for Slit2 down-regulation and Slit2-induced endocytosis of Robo1. Preliminary data suggests that Slit2-induced endocytosis of Robo1 may be required for downstream signaling. These findings have important implications for how Slit/Robo signaling may be dynamically regulated during cell migration. Receptor-mediated endocytosis Slit Robo Cell migration 0379 0307
3	Identification of a Peptide Sequence That Improves Transport of Macromolecules Across the Intestinal Mucosal Barrier Targeting Goblet Cells Kang, Sang, Woo, Jung Hee, Kim, Min Kook, Woo, Sang Soo, Choi, Jin Hyuk, Lee, Hong Gu, Lee, Nam Kyung, Choi, Yun Jaie 01 June 2008 (has links) In this study, we demonstrated that the CSKSSDYQC-peptide ligand which was identified from a random phage-peptide library through an in vivo phage display technique with rats could prominently improve the transport efficiency of macromolecules, such as large filamentous phage particles (M13 bacteriophage), across the intestinal mucosal barrier. Synthetic CSKSSDYQC-peptide ligands significantly inhibited the binding of phage P1 encoding CSKSSDYQC-peptide ligands to the intestinal mucosal tissue and immunohistochemical analysis showed that the CSKSSDYQC-peptide ligands could be transported across the intestinal mucosal barrier via goblet cells as their specific gateway. Thus, we inferred that CSKSSDYQC-peptide ligand might have a specific receptor on the goblet cells and transported from intestinal lumen to systemic circulation by transcytosis mechanism. These results suggest that CSKSSDYQC-ligand could be a promising tool for development of an efficient oral delivery system for macromolecular therapeutics in the carrier-drug conjugate strategy. goblet cell intestinal mucosa oral drug delivery system phage display receptor-mediated endocytosis transcytosis
4	Characterization of the endocytic pathways regulating riboflavin (vitamin B2) absorption and trafficking in human epithelial cells Foraker, Amy Beth 08 March 2007 (has links) No description available. riboflavin receptor-mediated endocytosis clathrin dynamin 2 GTPase caveolin 1 human epithelia
5	Insights into the Host Cell Entry of Ehrlichia chaffeensis: Roles of the Bacterial Outer Membrane Protein EtpE Mohan Kumar, Dipu 15 September 2014 (has links) No description available. Immunology Microbiology Medicine Ehrlichia chaffeensis Human monocytic ehrlichiosis EtpE DNase X GPI-1anchored protein receptor, ligand invasin receptor-mediated endocytosis CD147 hnRNP-K actin cytoskeletal mobilization vaccine N-WASP ROS NADPH Oxidase lysosomes vaccine

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