The interaction of osteoclasts and osteoblasts regulates bone density and the maintenance of osseointegration of implants. To investigate osteoblastic response to strain, rat osteosarcoma cells were cultured on titanium plates and subjected to one of three treatments: a neutral control (0 microstrain), tension (1000 microstrain), and compression (1000 microstrain). Treatments were applied via 4 point bending for fifteen minutes each day to a strain of 1mm/m at a sinusoidal frequency of 1Hz. Cells were measured for DNA as indicator of proliferation, alkaline phosphatase as indicator of phenotype and total protein and calcium deposition as indicators of matrix formation. Results indicated that DNA and total protein differed significantly between treatments. The alkaline phosphatase levels and calcification of matrix did not. The differences in the levels of DNA indicate different growth patterns between treatments. The total protein levels imply distinctions in matrix deposition. The alkaline phosphatase and calcium levels did not vary significantly, implying no difference in bone-specificity or calcification.

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京都大学 / 新制・課程博士 / 博士(医学) / 甲第23797号 / 医博第4843号 / 新制||医||1058(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 川口 義弥, 教授 藤田 恭之, 教授 小濱 和貴 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

The purpose of this thesis is to examine the possibilities for the revitalization of an abandoned railroad station and to derive from the project significant issues and strategies which can be applied to any reuse project. / Master of Architecture

This Thesis is aimed at evaluating the options of renovation for an existing residential building to make it more energy efficient. The various aspects in the basic structures of residential homes are discussed in order to help the user identify the areas of the house for which renovation is required to improve the energy efficiency of the building. These aspects include doors, roof and wall in addition to various systems of electrical wiring, mechanical systems of ventilation, heating and cooling and plumbing systems for the efficient flow of water throughout the house. The renovation options have been described in detail to provide as many possibilities to the user as possible. The building taken for renovation is a 1953 suburban home which has been awarded the honor of being the first building to be labeled as Zero Energy Home in its vicinity. This has made the home so efficient that its expenditure of energy has become equivalent to its energy generation, therefore, cancelling each other out and creating an estimate of zero energy.