Protein Nkr-p1b is a surface receptor of cytotoxic NK cells, that mediates inhibitory signal toward the body's own cells. In this study, the ligand binding domain of the mouse protein receptor Nkr-p1b (mNkr-p1b LBD) was prepared by recombinant expression in E. coli cells. Isolated protein was subsequently used for NMR structural analysis. Prediction of protein secondary structures ratio was carried out using three different methods (CD, PSIPRED and TALOS). Results correlate well with the structure of CTLD domain, that plays a key role in ligand binding and thus to function of Nkr-p1b receptor. We managed to prepare this protein in a form suitable for NMR experiments. Based on the data obtained by NMR spectra analysis, a preliminary model of the mNkr-p1b LBD protein structure was created. However, for more precise learning of the 3D structure accurate positions of individual atoms need to be determined by other NMR spectra evaluation in the next phase. Explaining the structure of the ligand binding domain of mNkr-p1b protein could help to better understand the complex mechanism of activation of NK cell cytotoxic activity, thereby contributing to its controlled use as a therapeutic against some viral and tumor diseases.

The main objective of this work is to promote the reinsertion of urban water in the context of physical and territorial planning, in the cities, through the study of the watershed of Jataí stream in Uberlândia-MG. Pursuiting the rebalancing of the waters with the urban environment, this work promote the connectivity and physical strengthening of the relations between man and environment / nature. The study analyzed the environmental impacts generated in the watershed with the process of urbanization and infrastructure works carried out in the stream and surrounding areas. Simulation of 4 (four) scenarios were proposed and hydrological parameters were raised in a natural conditions (before the urbanization process), as far as current urbanization, complete urbanization and ideal urbanization. To develop this ideal scenario, direct and preventive urban interventions were proposed, through the inclusion of an ecological and sustainable urban design, with the use of green infrastructure, such as parks and green areas, based on the principles of an environmental planning and structured in an Ecological-Economic Zoning (EEZ) of the watershed of Jataí stream. The results were compared and evaluated in an integrated manner, with the use of remote sensing techniques and the guidelines adopted showed a positive result in the hydrological simulations, with the significant increase in permeability, time of concentration and reduction of peak flows, showing, respectively, in the scenarios A, B, C and D the following values: 2,81 m3/s, 19,70 m3/s, 51,24 m3/s, and 15,15 m/3s.Thus, it is understood that, through the control of the permeation rates, considering only the areas of public use, such interventions are sufficient to promote significant effects on the control of direct runoff and flooding, allowing the minimization of environmental impacts. / O objetivo principal deste trabalho foi promover a reinserção das águas urbanas no contexto do planejamento físico-territorial nas cidades, por meio do estudo da microbacia do córrego Jataí, na cidade de Uberlândia-MG, com a busca do reequilíbrio das águas com o meio urbano, promovendo a conectividade e o fortalecimento das relações físicas e ambientais entre homem/natureza. O estudo analisou os impactos ambientais gerados na microbacia com o processo de urbanização e as obras de infraestrutura realizadas no córrego e no entorno. Foram propostas as simulações de 4 (quatro) cenários e levantados os parâmetros hidrológicos na condição natural (anterior ao processo de urbanização), urbanização atual, urbanização completa e urbanização ideal. Para elaboração do cenário ideal, foram propostas diretrizes para intervenções urbanas preventivas e diretas, por meio da inserção de um desenho urbano ecológico e sustentável, com a inserção de parques e áreas verdes e a utilização de infraestruturas verdes, baseadas nos princípios de planejamento ambiental e estruturadas em um Zoneamento Ecológico-Econômico (ZEE) da microbacia do córrego Jataí. Os resultados foram comparados e avaliados de forma integrada com a utilização de técnicas de sensoriamento remoto, e as diretrizes adotadas apresentaram um resultado positivo nas simulações hidrológicas, com o aumento significativo das taxas de permeabilidade, aumento no tempo de concentração e a diminuição dos picos de vazões, apresentando respectivamente nos cenários A, B, C e D, os seguintes valores: 2,81 m³/s, 19,70 m³/s, 51,24 m³/s e 15,15 m³/s. Assim entende-se que por meio do controle da taxa de permeabilidade considerando apenas as áreas destinadas ao uso público, são suficientes para promoverem efeitos significativos no controle do escoamento superficial direto e inundações, permitindo a minimização dos impactos ambientais. / Mestre em Engenharia Civil

Ziel der vorliegenden Bachelorarbeit war es, die Grundwasseraustritte im Forstrevier Ottomühle zu erfassen und zu bewerten, um Handlungsempfehlungen für die Renaturierung oder zukünftige Pflege der Quellbereiche und die forstliche Bewirtschaftung im Umfeld erarbeiten zu können. Zwischen März und Juni 2020 konnten insgesamt 19 Quellen im Forstrevier Ottomühle auf Grundlage einer Handlungsanleitung des Bayrischen Landesamtes für Umwelt erfasst und nach ihrer Naturnähe bewertet werden. Die vorgefundenen Beeinträchtigungen waren in absteigender Häufigkeit (Anzahl der betroffenen Quellen): • Nicht standortgerechter Baumbestand (12) • Kronenmaterial oder Schlagabraum (9) • Trittschäden (8) • Aufstau (5) • Kahlschlag (3) • Oberflächen- oder Drainagewassereinleitung (3) • Uferverbau oder Sohlenverbau (3) • Verrohrung (3) • Totalverbau (2) • Umleitung oder Verlegung (2) Auf Basis aktueller Literatur wurde anschließend für jede Quelle eine Handlungsempfehlung erarbeitet. Sie beinhaltet Maßnahmenvorschläge, Renaturierungsempfehlungen sowie Pflege- und Bewirtschaftungshinweise für den Quellbereich und das nähere Umfeld.:1 EINLEITUNG 2 MATERIAL UND METHODEN 2.1 UNTERSUCHUNGSGEBIET 2.2 SYSTEMATIK DER QUELLEN 2.3 DATENAUFNAHME IM FREILAND 2.3.1 Basis-Bogen 2.3.2 Detail-Bogen 2.4 QUELLENBEWERTUNG 2.5 ERARBEITUNG DER HANDLUNGSEMPFEHLUNG 3 ERGEBNISSE 3.1 ALLGEMEINE AUSWERTUNG 3.2 EINZELQUELLEN 3.2.1 „Wegquelle“ (ID 00001) 3.2.2 Zehrborn (ID 00002) 3.2.3 „Sumpfquelle“ (ID00003) 3.2.4 „Eisenquelle“ (ID 00004) 3.2.5 „Flurquelle“ (ID 00005) 3.2.6 Singeborn (ID 00006) 3.2.7 „Turmquelle“ (ID 00007/00008) 3.2.8 „Wildquelle“ (ID 00009) 3.2.9 „Teichquelle“ (ID 00010) 3.2.10 „Kapuzinerquelle“ (ID 00011) 3.2.11 Herkulesquelle (ID 00012) 3.2.12 „Johannisquelle“ (ID 00013) 3.2.13 „Kachequelle“ (ID 00015) 3.2.14 „Fichtenquelle“ (ID 00016) 3.2.15 „Jägerquelle“ (ID 00017) 3.2.16 „Brennnesselquelle“ (ID 00018) 3.2.17 „Fallenquelle“ (ID 00019) 3.2.18 „Dornquelle“ (ID 00020) 3.2.19 „Hainquelle“ (ID 00021) 4 DISKUSSION 5 FAZIT ANHANG / The aim of this bachelor thesis was to register and evaluate the current state of coldwater springs in the forest district „Ottomühle“. The gathered data was used to develop recommendations for action, which include renaturation processes, future maintenance of the springs and forest management in the surrounding areas. From March to June 2020 a total of 19 springs were registered in the district. The collection of needed parameters and the proximate assessment according to a springs natural state followed the instruction manual of the Bavarian State Office for the Environment (Bayrisches Landesamtes für Umwelt). The impairments found were in decreasing frequency (number of affected springs): • Non-natural tree population (12) • Branch material or low-grade timber (9) • Soil damage by trampling or vehicles (8) • Water impoundment (5) • Forest clearance (3) • Waterinput from surface or drainage water (3) • Piping (3) • Obstruction of riverbanks or riverbed sheeting (3) • Complete obstruction (2) • Bypass or relocation (2) The recommendations for further action in relation to impairments are presented in this paper.:1 EINLEITUNG 2 MATERIAL UND METHODEN 2.1 UNTERSUCHUNGSGEBIET 2.2 SYSTEMATIK DER QUELLEN 2.3 DATENAUFNAHME IM FREILAND 2.3.1 Basis-Bogen 2.3.2 Detail-Bogen 2.4 QUELLENBEWERTUNG 2.5 ERARBEITUNG DER HANDLUNGSEMPFEHLUNG 3 ERGEBNISSE 3.1 ALLGEMEINE AUSWERTUNG 3.2 EINZELQUELLEN 3.2.1 „Wegquelle“ (ID 00001) 3.2.2 Zehrborn (ID 00002) 3.2.3 „Sumpfquelle“ (ID00003) 3.2.4 „Eisenquelle“ (ID 00004) 3.2.5 „Flurquelle“ (ID 00005) 3.2.6 Singeborn (ID 00006) 3.2.7 „Turmquelle“ (ID 00007/00008) 3.2.8 „Wildquelle“ (ID 00009) 3.2.9 „Teichquelle“ (ID 00010) 3.2.10 „Kapuzinerquelle“ (ID 00011) 3.2.11 Herkulesquelle (ID 00012) 3.2.12 „Johannisquelle“ (ID 00013) 3.2.13 „Kachequelle“ (ID 00015) 3.2.14 „Fichtenquelle“ (ID 00016) 3.2.15 „Jägerquelle“ (ID 00017) 3.2.16 „Brennnesselquelle“ (ID 00018) 3.2.17 „Fallenquelle“ (ID 00019) 3.2.18 „Dornquelle“ (ID 00020) 3.2.19 „Hainquelle“ (ID 00021) 4 DISKUSSION 5 FAZIT ANHANG