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The aim of the thesis was to analyse the reproductive efficiency of breeding geese in Fisheries Nové Hrady s.r.o. for the established period. The biggest difference in terms of fertilized eggs (P < 0.01) was observed between the objects of the Lomský předkmeny and Karolínský. A statistically significant difference of 13.42 % (P < 0.05) was among the objects of the Lomský předkmeny and Byňovský. The difference of fertilized eggs between the years 2016 and 2017 was of 25.64%, statistically highly significant (P < 0.01). The highest hatchability of setting eggs was detected in the object Karolínský. Was about of 15.01 % (P < 0.01) higher than in the object Lomský předkmeny, respectively. Byňovský (36.52%). In 2017 there was a large increase in hatchability of setting eggs of 24.95 % (P < 0.01), in comparison with the year 2016. The results of the hatchability of fertile eggs were similar. The biggest difference was recorded between the objects Byňovský and Karolínský. Difference 5.06% was not statistically significant. Differences in hatchability of fertile eggs have been between the object Lomský předkmeny and Karolínský, respectively Byňovský around 2.5 %. From the point of view, hatchability of fertile eggs was between the year 2016 and 2017, a statistically significant difference (7.48%, P < 0.01). The highest fertility of eggs were found in March, in April dropped by 15.82%, in May dropped by 11.60% and in June the decrease was only about 1.19%. A statistically significant effect (P < 0.05) were confirmed between fertility in March and May or June. Towards the end of the laying period by reducing the biological value of eggs occurred and to reduce the hatchability of setting eggs. Hatchability of setting eggs decreased in April by 13.75%, in May of 9.52% and in June only 0.78%. Differences in fertility between March and May or June was statistically significant (P < 0.05). For two-years-old geese, compared with one-years-old geese, fertility eggs fell by 29.1%, for three-years-old geese increased by 34.24% and four-years-old geese have declined by 18.75%. The difference between the two-years-old geese and one-years-old geese, respectively, 3-years-old geese were statistically significant (P < 0.05).

The characteristics of energy status in porcine oocytes as related to their meiotic competence and in vitro maturation were studied. Cycling pubertal gilts in the early luteal to the early follicular phases of the ovarian cycle were used as oocyte donors. The oocytes recovered from medium (MF) or small follicles (SF) were considered meiotically more or less competent, respectively. The oocytes were examined before or after maturation by confocal microscopy, a bioluminescent cell assay and Western blotting. Four experiments, each in triplicate, were performed to assess both SF and MF oocytes in terms of metabolic units formed by mitochondria and lipids, ATP and lipid consumption and lipid droplets with adipose differentiation-related protein (ADRP) expression. In conclusion, specific differences in energy characteristics between porcine oocytes with different meiotic competence were found. Meiotically more competent oocytes are more advanced in terms of energy reserves before maturation, while meiotically less competent oocytes are more active in replenishing energy stores during maturation.