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Bacillus cereus is a ubiquitously found foodborne pathogen that is frequently associated with two types of illness: emesis and diarrhea. Two heat labile enterotoxins have been associated with the diarrheal syndrome whereas a heat stable acid stable peptide toxin has been associated with the emetic syndrome. In the U.S, B. cereus is responsible for 1-2% of the total outbreaks from bacteria. Although there are reports of isolation and characterization of this pathogen from various food stuffs all around the world, there are no reports on the levels, toxin producing ability, or growth characteristics from U.S retail rice. Considering that rice is grown mostly in developing countries and most of the rice in the U.S is imported, there is a high chance of the rice being contaminated with B. cereus spores. Therefore, the major objective of this thesis was to characterize B. cereus spores from U.S retail rice. The levels were determined and further the enterotoxigenic ability and the growth characteristics along with the physical characteristics of the isolates were studied. Among the 178 samples analyzed, Spores of Bacillus species were found in 94 (52.8%) of the rice samples with an average concentration of 32.6 CFU/g (3.6-460 CFU/g). Eighty nine of the 94 isolates were tested positive for one of the two enterotoxins produced by B. cereus. none of the 94 isolates tested positive for the emetic gene. All the isolates generally grew well in cooked rice. Levels of 106/g were detected in cooked rice after 22h at 200C and after 34h at 170C whereas at 120C the counts did not go above 104/g even after 48h. A significant difference in the heat resistance of the emetic and the diarrheal strains was found. The emetic but not the diarrheal type grew well at inoculum levels of 102/g and 103/g level following cooking. So these results suggest although the diarrheal type are more predominant in U.S retail rice, the chances of foodborne illness arising from the diarrheal strains is low. B. cereus, B, thuringiensis and B. mycoides were investigated for their physical characteristics. Appendages were not found on B. mycoides. By contrast, all the isolates had exosporia. The isolates were characterized to be moderately to highly hydrophobic and all the isolates had a net negative charge. Judging by their physical characteristics, it can be concluded that these spores may have a high affinity for adhering to inert surfaces.

Research was conducted at the Mississippi State University Delta Research and Extension Center from 2016 to 2018 to determine the effects of sub-lethal concentrations of paraquat, glyphosate, saflufenacil, and sodium chlorate exposure to rice at late-season growth stages, determine the effects of exposure to sub-lethal concentrations of glyphosate or paraquat on multiple rice cultivars, and characterize the effects of paraquat exposure and Oebalus pugnax feeding on rice grain quality. In the current research, rough rice grain yields were reduced by exposure to sub-lethal concentrations of soybean desiccants 0 to 28 DAH. Rice injury was determined to not be an accurate predictor of rough rice grain yield loss as injury did not exceed 20% with any desiccant, and no injury was observed from glyphosate applications. Rough rice grain yield reductions were reflected in yield component reductions following desiccant exposure. Applications of sub-lethal concentrations of glyphosate or paraquat to rice at 50% heading caused rough rice grain yield decreases ranging from 0 to 20 and 9 to 21 % respectively. Hybrid cultivars were unaffected following glyphosate exposure at 50% heading. In the current research, observations of paraquat exposure or O. pugnax infestation of rice at the soft dough growth stage suggest rice may exhibit severe sensitivity to both events in the form of reduced kernel weight and reductions in rice milling quality. Rough rice grain yield reductions coupled with milling quality reductions and driven by the proximity of rice to corn, cotton, soybean, and sorghum in Mississippi creates the need to exercise caution when applying desiccants.

Waste rice in harvested fields and natural seeds in moist-soil wetlands are important foods for waterfowl in the Mississippi Alluvial Valley (MAV). Waste rice declines significantly between harvest and late autumn. I conducted experiments in 19 rice fields in Arkansas and Mississippi during autumns 2003 and 2004 to evaluate the ability of common post-harvest practices to conserve waste rice between harvest and late autumn. Greatest mean abundance of waste rice in late autumn occurred in standing stubble (x&95 = 105 kg/ha; CL = 72.84, 150.16 kg/ha) followed by burned (x&95 = 72 kg/ha; 49.57, 105.81 kg/ha), mowed (x&95 = 67 kg/ha; 46.65, 97.42 kg/ha), rolled (x&95 = 51 kg/ha; 35.54, 73.076 kg/ha), and disked stubble (x&95 = 48 kg/ha; 33.26, 68.41 kg/ha). I recommend leaving stubble or burning fields to create interspersion of stubble and water after flooding. Additionally, I estimated abundance of moist-soil seed in 72 management units on public lands throughout the MAV for autumns 2002-2004. Mean seed abundance was 496 kg/ha (SE = 62). I recommend increased active management of moist-soil wetlands to mitigate decreased availability of waste rice in the MAV.

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