Background and Problem Pakistan's clothing industry plays an important role in the country's economy; more than 60% of total exports and job possibilities are provided by the clothing industry in Pakistan. Small and medium-sized industries (SMEs) have increased in recent years, contributing to the industry's overall development (SMEs) throughout the last decade. Pakistan's clothing industry has grown rapidly but faces significant challenges of overpricing and technological roadblocks. Overpricing makes clothing items unaffordable for consumers, while outdated technology and machinery cause issues in productivity and competitiveness. Purpose The purpose of this study is to investigate the two challenges of entrepreneurs which are overpricing and technological roadblocks in the Pakistan clothing industry and to provide their solutions. Research Questions The following research questions are created to accomplish the purpose and justify this study:1. How entrepreneurs in the Pakistan clothing industry are facing challenges due to overpricing?2. What are the technological challenges in the Pakistan Clothing industry for entrepreneurs?3. What are the solutions to overpricing and technological roadblocks in the clothing industry of Pakistan? Methodology The research follows a qualitative research method while the deductive approach has been used. The study's main goal is to achieve its objective and solve the research question by utilizing both primary and secondary data. The primary data collection involved conducting four in-depth interviews with3officials of the Pakistan clothing industry. Secondary data was gathered from different sources such as scientific journals, articles, books, and websites to complement the primary data and provide a comprehensive analysis. Findings The finding of this research is that entrepreneurs in the clothing industry face overpricing challenges due to the rising cost of production, the rising cost of raw materials, and the high cost of skilled labor. In technological challenges, the most common challenges entrepreneurs face are outdated machinery, lack of investment in technology, lack of research and development, poor quality, and lack of digital tools. The study also suggests some solutions to these challenges: Sustainable Sourcing, Government Support, Investment in Technology, focused on market research, enhancing the product's value proposition by reducing production costs, Programs for Technology Education, and Training Partnerships with Technology Providers and Consultants Limitations This research conducts only four interviews in Pakistan for primary data collection. The cross-sectional, non-experimental design of the study was a limitation as it only allowed for data to be collected at a single time point.

Proteins have been optimized by evolution for billions of years to work on a nanometer scale. Therefore, they are extremely promising for nanotechnological applications. Cytoskeletal filaments propelled by surface-attached motor proteins have been recently established as versatile transport platforms for nano-sized cargo in molecular sorting and nano-assembly devices. However, in this gliding motility setup, cargo and motors share the filament lattice as a common substrate for their activity. Therefore, it is important to understand the influence of cargo-loading on transport properties. By performing single molecule stepping assays on biotinylated microtubules, it was shown that kinesin-1 motors first stop and then detach when they encounter a streptavidin obstacle on their path along the microtubule. Consequently, the deceleration of streptavidin coated microtubules in gliding assays could be attributed to an obstruction of kinesin-1's path on the microtubule rather than to "frictional" streptavidin-surface interactions. The insights gained by studying kinesin-1's behavior at obstacles were then used to demonstrate a novel sensing application: Using a mixture of two distinct microtubule populations that each bind a different kind of protein, the presence of these proteins was detected via speed changes in the respective microtubule populations. In future applications, this detection scheme could be combined with other recent advancements in the field, creating highly integrated lab-on-a-chip devices that use microtubule based transport to detect, sort and concentrate analytes. It has been envisioned that the kinesin-1-microtubule system could be used for even more complex appliances like nano-assembly lines. However, currently available control mechanisms for kinesin-1 based transport are not precise enough. Therefore, improved temporal control mechanisms for kinesin-1 were investigated: Using a polymer that changes its size in solution with temperature, starting and stopping of gliding microtubules was demonstrated. In combination with local heating by light, this effect could be used to control the gliding of single microtubules. Finally, a strategy to create photo-switchable kinesin-1 was developed and tested for feasibility using molecular modeling.

Proteins have been optimized by evolution for billions of years to work on a nanometer scale. Therefore, they are extremely promising for nanotechnological applications. Cytoskeletal filaments propelled by surface-attached motor proteins have been recently established as versatile transport platforms for nano-sized cargo in molecular sorting and nano-assembly devices. However, in this gliding motility setup, cargo and motors share the filament lattice as a common substrate for their activity. Therefore, it is important to understand the influence of cargo-loading on transport properties. By performing single molecule stepping assays on biotinylated microtubules, it was shown that kinesin-1 motors first stop and then detach when they encounter a streptavidin obstacle on their path along the microtubule. Consequently, the deceleration of streptavidin coated microtubules in gliding assays could be attributed to an obstruction of kinesin-1's path on the microtubule rather than to "frictional" streptavidin-surface interactions. The insights gained by studying kinesin-1's behavior at obstacles were then used to demonstrate a novel sensing application: Using a mixture of two distinct microtubule populations that each bind a different kind of protein, the presence of these proteins was detected via speed changes in the respective microtubule populations. In future applications, this detection scheme could be combined with other recent advancements in the field, creating highly integrated lab-on-a-chip devices that use microtubule based transport to detect, sort and concentrate analytes. It has been envisioned that the kinesin-1-microtubule system could be used for even more complex appliances like nano-assembly lines. However, currently available control mechanisms for kinesin-1 based transport are not precise enough. Therefore, improved temporal control mechanisms for kinesin-1 were investigated: Using a polymer that changes its size in solution with temperature, starting and stopping of gliding microtubules was demonstrated. In combination with local heating by light, this effect could be used to control the gliding of single microtubules. Finally, a strategy to create photo-switchable kinesin-1 was developed and tested for feasibility using molecular modeling.

The purpose of the present study was to gain more knowledge about single individuals with specific premarital risk factors for later marital problems (e.g. neuroticism or low emotional readiness, family-of-origin dysfunction, poor communication skills, and hostile conflict resolution style). The sample (N=39) completed the RELATionship Evaluation (RELATE) and were selected for the study based on the presence of one or more risk factors. Participants were contacted via email and asked to complete an online version of the Relationship Follow-up Questionnaire (RFQ) to determine their knowledge or awareness of their risk factor(s) after taking RELATE, their motivation for premarital interventions after taking RELATE, their perceived roadblocks or barriers hindering participation in premarital interventions, and their post-RELATE marriage preparation behaviors including their post-RELATE relationship breakups. The results showed that the RELATE increased an at-risk individual's knowledge or awareness of risk factors, increased their motivation for premarital interventions, and had little to no effect on the break-up of relationships. At-risk individuals reported the major roadblocks or barriers to participation in premarital interventions as being time, money, being uninformed, and the perceived distance/inconvenience of interventions. At-risk individuals' post-RELATE behaviors included discussing the RELATE results with their partner and friends and utilizing books and articles related to marriage. They did not report utilizing individual or couples counseling. Practitioners can use this information to make premarital interventions more appealing to at-risk individuals and encourage the use of the RELATE to increase participation in premarital interventions and help prevent later marital problems.