The thesis deals with hygiene of educational process and sources of school strain. Theoretical part describes learning, socialization, human needs, specifics and risk of puberty, school strain and its sources, nature of reactions to stress, issue of class climate and abuse. Part of theoretical part deals with prevention and health promotion. Two main objectives have been determined: 1. Map subjective perception of each factors of hygiene of educational process, 2. Investigate the most common sources of school strain which influences health of ninth-grade pupils. Quantitative research methods have been selected for the practical part. Data was obtained through anonymous questionnaire. The questionnaire consists of twenty questions, the first two are identifiable (gender, age) and the remaining eighteen questions are focused on fading of subjective perceptions of the factors of the educational process. Preliminary research on twenty respondents was made before the start of the survey. Collection of questionnaires was made in February and March 2013. Data were evaluated in MS Excel and the results are presented in graphical and tabular form. The research group consisted of ninth-grade pupils of primary schools in Pardubice. The sampling frame was selected by simple random sampling. Each fifteen primary schools were properly numbered and then five of them were drawn lots of a hat. In total, 300 questionnaires were distributed. Although the response rate was 100%, 68 questionnaires remained unfilled, so 232 copies (77%) was evaluated in the practical part. Of the 232 respondents,106 respondents (46%) registered male and 126 respondents (54%) enrolled female. Age of probands was between fourteen into sixteen years. The paper outlines two hypothesis: H1. The boys are satisfied with the quality of the physical factors of the educational process more than girls, H2. The girls suffer subjective health problems more often than boys. For testing of both hypothesis was selected two sample test of significance for the mean value of two sample sizes (Student´s t-test). To verify H1 was necessary to use evaluation of four closed questions related to physical factors of the educational process (illumination, noise, air quality, air temperature). Testing of H2 was mediated by data obtained from question that map frequency of health problems.Because there is no difference between the sexes in the studied phenomena, the expected hypothesis were rejected. The results of this thesis show that pupils are more dissastified with the quality of school furniture than with the quality of physical factors. Non-school breaks from teachers is a fundamental problem. More than 50% of respondents recorded this adverse effect. The issue of homework can not be ignored neither. More than 46% of pupils record having a homework every day or several times a week. Perceptions of pupils that are recorded in the results of thesis and then confronted in discussion may be used as a source of informatiion for teachers of primary schools who will be able to compare the results of the research with data obtained from their primary school.

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