TNT (2,4,6-trinitrotoluene) is a persistent contaminant at many military installations and poses a threat to aquatic ecosystems. Data from environmental fate and toxicity studies with TNT revealed that sediment toxicity test procedures required modification to accurately assess sediment TNT toxicity. Key modifications included aging TNT-spiked sediments 8-14 d, basing lethal dose on measured sediment concentrations of the molar sum of TNT and its main nitroaromatic (NA) transformation products (SNA), basing sublethal dose on average sediment SNA concentrations obtained from integration of sediment SNA transformation models, avoiding overlying water exchanges, and minimizing toxicity test durations. Solid phase microextraction fibers (SPMEs) were investigated as a biomimetic chemical measure of toxicity and bioavailability. Both organism and SPME concentrations provided measures of lethal dose independent of exposure scenario (TNT-spiked sediment or TNT-spiked water) for Tubifex tubifex. Among all benthic organisms tested (Chironomus tentans, Ceriodaphnia dubia, T. tubifex) and matrixes, median lethal dose (LC50) estimates based on SPME and organism concentrations ranged from 12.6 to 55.3 mmol SNA/ml polyacrylate and 83.4 to 172.3 nmol SNA/g tissue, ww, respectively. For Tubifex, LC50s (95% CI) based on SNA concentrations in sediment and SPMEs were 223 (209-238) nmol SNA/g, dw and 27.8 (26.0-29.8) mmol SNA/ml, respectively. Reproductive effects occurred at slightly lower exposures. Median effective dose (EC50) estimates (95% CI) for Tubifex cocoon production, based on sediment and SPME concentrations, were 118 (114-122) nmol SNA/g, dw and 21.8 (21.2-22.4) mmol SNA/ml, respectively. Bioconcentration experiments with Tubifex revealed that compound hydrophobicity predicted the toxicokinetics and bioconcentration of these compounds from water, however, there was a large discrepancy between the toxicokinetics of absorbed versus metabolically-generated aminodinitrotoluenes. A large portion of bioconcentrated, radiolabeled TNT transformation products could not be identified. In addition to their ability to provide matrix-independent measures of dose, SPME concentrations were more accurate indicators of bioavailable NAs than were sediment concentrations.

The Friis Hills is an isolated plateau standing as much as 600 m above surrounding topography in the McMurdo Dry Valleys region or Antarctica.Preserved on the plateau surface is a sequence of early to middle Miocene-aged dritis. At the eastern edge of the plateau, these drifts fill a shallow paleovalley to a depth of at least 35 m. The drills are exposed in a natural cross-section where modern topography crosscuts the paleovalley. Establishing an age and an environmental interpretation for these deposits is important because Antarctic paleoclimate records are lacking from the Mid-Miocene Climate Optimum. Two drifts fill the ancient paleovalley in the eastern Friis Hills. The upper drift is here named Cavendish drift: the lower is here named Friis drift. Cavendish can be subdivided into three units, whereas Friis drift can be subdivided into two units. Each of these units is a horizontal bed that laps on paleovalley sidewalls. The lowest, Friis II, is a compact diamicton that is overlain by a nearly in-situ bedded volvanic ash. Based on [20]Ar/[39]Ar dating, the ash is 19.76 [plus/minus] 0.07 Ma old. A second diamicton, Friis I, conformably blankets Friis II and was discovered to hold fossileferous interbeds. Both Friis I and II contain erratic clasts and both are lodgemont tills deposited from small, locally derived, alpine glaciers. Bedrock striations show ice flow to the northeast at azimuths between 025? to 032?, parallel to the trend of the paleovalley axis. Above these, Cavendish I. II. and III were deposited when thick ice covered the Friis Hills. Where the Cavendish drift laps onto paleovalley sidewalls, bedrock striations show ice flow from 077? to 150?. Cavendish drift was deposited sometime alter 19.8 Ma but before 14 Ma. when the Dry Valleys glacial records show that regional glaciers became cold-based. Downcutting eventually isolated the Friis Hills plateau, resulting in the preservation of the drift sequence. This event was most likely associated with growth or the East Antarctic Ice Sheet 14 Ma ago. This age constraint means that the tills preserved in the Friis Hills date from a time just before the East Antarctic Ice Sheet expanded and became a permanent feature. Based on the age-dated stratigraphy presented in this thesis, future work focusing on fossiliferious interbeds could provide unique and important constraints on Miocene climate change. / North Dakota State University. Department of Geosciences

Thesis (Ph. D.)--Joint Program in Oceanography (Massachusetts Institute of Technology, Dept. of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), 2007. / Includes bibliographical references. / Basaltic ocean crust has the potential to host one of the largest endolithic communities on Earth. This portion of the biosphere, however, remains largely unexplored. In this study, we utilize molecular biological, microscopic, and geochemical tools to gain a better understanding of the geomicrobiology of the ocean crust. Specifically, we examine the phylogenetic diversity of microorganisms inhabiting basaltic lavas, the activities and abundances of these microorganisms, the spatial extent of the biosphere, and the potential effect that microbial activity has on the geochemistry of the ocean crust and overlying water column. Our study demonstrates that young, fresh volcanic lavas near mid-ocean ridges host an incredibly diverse and dense population of microorganisms dominated by Bacteria, quite distinct from the microbial communities found in surrounding deep seawater and hydrothermal vents. Furthermore, these communities may contribute to the elemental cycling of Fe, S, Mn, N, and C in this environment. The inability to definitively identify microorganisms in drill-cores of old (> 15 Ma) ocean crust, however, implies that these once prolific communities may become scarce as the crust ages and moves further away from the ridge axis. Finally, we provide evidence suggesting that these communities are fueled by oxidative alteration reactions occurring in the basaltic crust. / by Cara M. Santelli. / Ph.D.

No description available.

No description available.

No description available.

The middle sandstone member of the Upper Triassic Chinle Formation in northeastern New Mexico consists of ephemeral-stream deposits adjacent to the Ancestral Rocky Mountain uplift and perennial braided-stream deposits on the plains to the east. The two fluvial systems were contemporaneous with the ephemeral rivers existing as transverse tributaries to a perennial trunk system flowing from north to south. Proximal ephemeral-stream deposits are characterized by 60-80 m thick sequences of coarse-grained sediments deposited principally by overbank sheetflooding processes. Facies associations are dominated by horizontally-stratified, medium-grained sandstone with subordinate sets of tabular-tangential and trough cross-stratified medium-grained sandstone. Streamflooding events are indicated by basal conglomeratic channelized deposits and single-story massive and trough cross-stratified channel sandstones. Uppermost portions of proximal ephemeral-stream sequences contain 0. 5 to 1. 0 m-thick units of trough cross-laminated, fine-grained sandstone and siltstone. Distal ephemeral-stream deposits are distinguished by their lenticular geometries, thicknesses of 20 to 30 m and sedimentary structures indicative of lower flow-regime streamflooding processes. Channelized conglomerates and trough cross-stratified and parallel-stratified sands~ones are capped by tabular and wedge cross-stratified, trough cross-laminated and subordinate horizontally-stratified sandstones. Facies associations in the deposits of the trunk system are comparable to those of the Platte and South Saskatchewan braided rivers (Miall, 1977). Sets and cosets of tabular-tangential or trough cross-stratified medium-grained sandstone are separated by variable thicknesses of horizontally-stratified medium-grained sandstone; these dominate 10 to 30 m-thick, sheet-like sandstones which are traceable laterally for tens of kilometers. The sheet-like sandstones are separated by equivalent or lesser thicknesses of red mudstones. Effects of the allocyclic controls of tectonics and climate are recognizable in sedimentary architectures of individual members or of the whole formation. In contrast, the effects of autocyclic controls can be recognized when internal geometries of individual members are focussed upon. The concept of base level is helpful in realizing the time dependency of controls on sedimentation because allocyclic controls determine the position of base level and base level determines which autocyclic controls operate. / M.S.

Tang Wai Lun. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2005. / Includes bibliographical references (leaves 199-220). / Abstracts in English and Chinese. / Abstract --- p.ii / 摘要 --- p.iv / Acknowledgements --- p.vi / Table of contents --- p.vii / List of Abbreviations --- p.xi / List of Tables --- p.xii / List of Figures --- p.xv / Chapter Chapter One --- Literature Review --- p.1 / Chapter 1.1 --- Marine pollution in Hong Kong --- p.1 / Chapter 1.2 --- Contamination of sediments --- p.3 / Chapter 1.2.1 --- Metals contamination in coastal sediments in Hong Kong --- p.4 / Chapter 1.2.2 --- Other contaminants in sediments --- p.7 / Chapter 1.3 --- Sediment toxicity assessment --- p.9 / Chapter 1.4 --- Fish as examples for the measurement of biological responses to contaminants --- p.9 / Chapter 1.5 --- Biomarkers for toxicity assessment --- p.11 / Chapter 1.5.1 --- Metallothionein (MT) --- p.11 / Chapter 1.5.2 --- Cytochrome P450 1A (CYP1A) --- p.13 / Chapter 1.5.3 --- Vitellogenin (Vtg) --- p.13 / Chapter 1.5.4 --- Enzyme biomarkers: Alanine transaminase (ALT); aspartate transaminase (AST) and creatine kinase (CK) --- p.14 / Chapter 1.5.5 --- DNA damage detected by the comet assay --- p.16 / Chapter 1.6 --- Multi biomarkers for sediment toxicity assessments --- p.16 / Chapter 1.7 --- Objectives --- p.16 / Chapter Chapter Two --- "Concentrations of Heavy Metals and Trace Organics in Coastal Sediments Collected from Victoria Harbour, Deep Bay, Tolo Harbour and Lai Chi Wo" --- p.18 / Chapter 2.1 --- Introduction --- p.18 / Chapter 2.2 --- Materials and Methods --- p.19 / Chapter 2.2.1 --- Sediment collection --- p.19 / Chapter 2.2.2 --- Treatment of sediment samples --- p.21 / Chapter 2.2.3 --- Acid wash of apparatus --- p.21 / Chapter 2.2.4 --- Acid digestion of sediment sample --- p.21 / Chapter 2.2.5 --- Sequential extraction of heavy metals in sediment samples --- p.22 / Chapter 2.2.6 --- Heavy metals measurement --- p.23 / Chapter 2.2.7 --- Trace organic measurement in sediments --- p.24 / Chapter 2.2.8 --- Statistical Analysis --- p.24 / Chapter 2.3 --- Results --- p.25 / Chapter 2.3.1 --- Concentrations of total Metals in sediments --- p.25 / Chapter 2.3.2 --- Speciation of metals in sediments --- p.27 / Chapter 2.3.3 --- Metal abundance in different fractions of sediment --- p.33 / Chapter 2.3.4 --- Concentrations of trace organic in sediments --- p.33 / Chapter 2.3.5 --- Results summary --- p.36 / Chapter 2.4 --- Discussion --- p.36 / Chapter 2.4.1 --- Comparison with international standards --- p.38 / Chapter 2.4.2 --- Comparison with international studies of coastal sediments --- p.39 / Chapter 2.4.3 --- Comparison of the present study with other studies concerning Hong Kong coastal sediments --- p.45 / Chapter Chapter Three --- "Heavy Metals Accumulation in Tissues of Tilapia Exposed to Coastal Sediments Collected from Victoria Harbour, Deep Bay, Tolo Harbour, and Lai Chi Wo" --- p.51 / Chapter 3.1 --- Introduction --- p.51 / Chapter 3.2 --- Materials and Methods --- p.52 / Chapter 3.2.1 --- Collection and treatment of coastal sediments --- p.52 / Chapter 3.2.2 --- Sediment exposure tests with Tilapia --- p.53 / Chapter 3.2.3 --- Dissection of fish samples --- p.54 / Chapter 3.2.4 --- Acid digestion of fish samples --- p.54 / Chapter 3.2.5 --- Statistical Analysis --- p.55 / Chapter 3.3 --- Results --- p.55 / Chapter 3.3.1 --- Metal accumulation --- p.55 / Chapter 3.3.2 --- "Dose, duration and source of sediments affecting metals uptake" --- p.95 / Chapter 3.3.3 --- Results summary --- p.99 / Chapter 3.4 --- Discussion --- p.99 / Chapter 3.4.1 --- Site comparison --- p.99 / Chapter 3.4.2 --- Comparison with other studies --- p.100 / Chapter 3.4.3 --- Safety limits --- p.112 / Chapter Chapter Four --- "Expression of Biomarker Genes (MT, CYP1 A, Vtg) in Tissues of Male Tilapia After Eexposure to Coastal Sediments Collected from Victoria Harbour, Deep Bay, Tolo Harbour and Lai Chi Wo" --- p.115 / Chapter 4.1 --- Introduction --- p.115 / Chapter 4.2 --- Materials and Methods --- p.116 / Chapter 4.2.1 --- Sample collection --- p.116 / Chapter 4.2.2 --- Preparation of apparatus --- p.117 / Chapter 4.2.3 --- mRNA expression analysis --- p.117 / Chapter 4.2.3.1 --- Isolation of RNA --- p.117 / Chapter 4.2.3.2 --- Reverse transcription --- p.118 / Chapter 4.2.3.3 --- Real time PCR --- p.118 / Chapter 4.2.4 --- Vitellogenin (Vtg) --- p.120 / Chapter 4.2.5 --- Statistics analysis --- p.121 / Chapter 4.3 --- Results --- p.121 / Chapter 4.3.1 --- MT mRNA expression in tissues of male Tilapia exposed to sediments --- p.121 / Chapter 4.3.2 --- CYP1A mRNA expression in tissues of male Tilapia exposed to sediments --- p.129 / Chapter 4.3.3 --- Vtg expression in tissues of male Tilapia exposed to sediments --- p.137 / Chapter 4.3.4 --- Dose and time dependent relationships --- p.137 / Chapter 4.3.5 --- Results summary --- p.137 / Chapter 4.4 --- Discussion --- p.142 / Chapter 4.4.1 --- Correlation of MT and CYP1A expression with metals uptake in gills and liver --- p.142 / Chapter 4.4.2 --- MT as a biomarker of metals exposure --- p.144 / Chapter 4.4.3 --- CYP1A as a biomarker of trace organics exposure --- p.145 / Chapter 4.4.4 --- Real-time PCR --- p.147 / Chapter 4.4.5 --- Vtg as a biomarker of xenoestrogen exposure --- p.147 / Chapter Chapter Five --- "Expression of Enzyme Biomarkers (ALT, AST and CK) in Tissues of Male Tilapia After Exposure to Coastal Sediments Collected From Victoria Harbour, Deep Bay, Tolo Harbour and Lai Chi Wo" --- p.149 / Chapter 5.1 --- Introduction --- p.149 / Chapter 5.2 --- Materials and Methods --- p.150 / Chapter 5.2.1 --- Samples collection --- p.150 / Chapter 5.2.2 --- Alanine transaminase (ALT) and Aspartate transaminase (AST) --- p.151 / Chapter 5.2.3 --- Creatine kinase (CK) --- p.152 / Chapter 5.2.4 --- Statistical analysis --- p.153 / Chapter 5.3 --- Results --- p.153 / Chapter 5.3.1 --- ALT --- p.153 / Chapter 5.3.2 --- AST --- p.157 / Chapter 5.3.3 --- CK in gills --- p.157 / Chapter 5.3.4 --- CK in muscle --- p.164 / Chapter 5.3.5 --- "Dose, duration and source of sediments affecting metals uptake" --- p.164 / Chapter 5.3.6 --- Results summary --- p.169 / Chapter 5.4 --- Discussion --- p.169 / Chapter Chapter Six --- "DNA Damage in Liver of Male Tilapia After Exposure to Coastal Sediments Collected from Victoria Harbour, Deep Bay, Tolo Harbour and Lai Chi Wo" --- p.174 / Chapter 6.1 --- Introduction --- p.174 / Chapter 6.2 --- Materials and Methods --- p.176 / Chapter 6.2.1 --- Samples collection --- p.176 / Chapter 6.2.2 --- Tissue Preparation --- p.176 / Chapter 6.2.3 --- Single Cell Gel Electrophoresis (Comet) Assay --- p.176 / Chapter 6.2.4 --- Image analysis --- p.177 / Chapter 6.2.5 --- Statistical analysis --- p.178 / Chapter 6.3 --- Results --- p.180 / Chapter 6.4 --- Discussion --- p.187 / Chapter Chapter Seven --- General Discussion and Conclusion --- p.190 / Chapter 7.1 --- General discussion --- p.190 / Chapter 7.2 --- Conclusion --- p.198 / References --- p.199 / Appendix --- p.221

The Klondike District of Central Yukon Territory around Dawson City is particularly advantageous for the study of the sequence of the Pleistocene epoch because it was never glaciated and therefore has never lost its fossiliferous superficial deposits. This paper presents a pollen diagram from a Sphagnum peat bed, which was the youngest deposit discovered, and pollen floras from different ages back to the Pliocene. The peat bed flora shows fairly steady climatic conditions little different from the present; the Pliocene flora shows a distinctly warmer climate; and all the others appear to show colder climates. The paper also presents a theory of correlation of geological events in the district with world-wide climatic variations: cutting of very broad creek valleys; climate warm - Earlier Tertiary - deposition of the oldest gravels; climate warm - Pliocene - deposition of oldest unweathered gravels; climate cold - Nebraskan Glaciation - rapid cutting of narrow lower creek valleys - Three Major Interglacial Ages - major interruptions of valley cutting; climate cold - Kansan and Illinoian Glaciation - deposition of thick valley - bottom muck; climate cold - Wisconsin Glaciation - erosion of valley - bottom muck; climate warmer than the present - Post-Glacial Climatic Optimum - deposition of Sphagnum peat bed; climate same as today - Recent Time [...]

Instrumental Neutron Activation Analysis (INAA) was used to identify geochemical groups in Boring Lava along the west side of the Tualatin Mountains, and in sediments of the Portland and Tualatin basins. Samples of Boring Lava were obtained from TriMet drill core collected during planning of the tunnel alignment for the Westside Light Rail line. Additional samples of Boring Lava were collected from outcrops along the west side of the Tualatin Mountains. Samples of sediment from the Tualatin and Portland basins were obtained from drill core collected during an Oregon Department of Geology and Mineral Industries (DOGAMI) Earthquake Hazards Mapping project. INAA of Boring Lava samples resulted in the identification of three geochemical groups. Additional data sets, including x-ray fluorescence geochemistry, magnetic polarity, and age dates, allowed for the distinction of three Boring Lava units. The Boring Lava of Barnes Road is a young, normal unit, the Boring Lava of Sylvan Hill is an older normal unit, and the Boring Lava of Cornell Mountain is the oldest, reversed unit. The surf ace distribution, identified using topography and outcrop geochemistry, is consistent with the subsurface distribution, identified using boring logs and core geochemistry. Volcanic vent locations are proposed at topographic highs within the identified surface distribution of the Boring Lava of Barnes Road. INAA of sediment samples resulted in the identification of seven groups: (1) Columbia River source sediments, (2) lower Troutdale Formation, (3) Reed Island ashes, (4) young Columbia River sediments, (5) highalumina basalt sediments, (6) episodic Cascadian volcanic sediments, and (7) Columbia River Basalt Group (CRBG) sediments. Only the CRBG sediments group was identified in the Tualatin basin, while all seven groups were identified in the Portland basin. This appears to demonstrate that the sediment packages in the two basins are different. Finally, each sediment group can be placed into one of three broad geochemical categories: Columbia River source sediments and lower Troutdale Formation represent a Columbia River or continental source; Reed Island ashes, young Columbia River sediments, high-alumina basalt sediments, and episodic Cascadian volcanic sediments represent a Cascadian or local source; and CRBG sediments represent residual soils or sediments overlying Columbia River basalt flows.