Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-14T17:34:23Z No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) / Made available in DSpace on 2017-02-14T17:34:23Z (GMT). No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) Previous issue date: 1997-09-30 / CNPq / This experimental work with Aedes aegypti L. (1762) was conducted in order to compare the effect of differents blood sources on feeding and egg production of mosquitoes? females. The blood-feeding device consisted of a plastic cage ? used for holding females during blood feeding ? with two lateral windows recovered with thin cloth to allow air exchange during feeding. In the top of the cage, there was an opening where the food?s container, recovered with silicone membrane, was attached. A larger vial filled with water at the initial temperature of 50 ?C was used over the apparatus to keep the food source warm (37,5 ? 0,5%). In each trial, three different treatments were tested: whole blood collected from mouse, bovine and human. To prevent coagulation, a 3,9% solution of sodium citrate was added to the blood. The control group was allowed to feed directly on human volunteers. The experiment was conducted in a climatic chamber, with temperature and humidity controlled at 28?0,5 ?C and 80?5%, respective values and the photoperiod of 12 hours of light and 12 hours of darkness. In each tested group, 30 females and 10 males mosquitoes were used. The females were allowed to feed during a period of 20-30 minutes and after that only the engorged females were transferred to another cages containing the oviposition sites. To evaluate the effect of different sources of blood on A. aegypti, the percentage of engorged females, pre-oviposition time and the number of eggs per engorged female were measured. Highest percentage of engorged females were among mosquitoes fed on human citrated blood (89%) and control (95%). The media oviposition on both treatments were 13,4 eggs for females fed on human citrated blood and 15,3 eggs for females of control group. The lots fed on mice and bovine citrated blood presented the following results respectively: 22,5% of engorged females and 9,2 eggs per female and 25,8% of engorged females and 10,3 eggs per female. / Foi comparado o efeito do sangue de camundongos, bovino e humano sobre o ingurgitamento e oviposi??o do mosquito Aedes aegypti L., 1762 (D?ptera:Culicidae). O aparato para alimenta??o artificial foi constitu?do por gaiolas de pl?stico, com 10 x 6 cm, com orif?cios laterais cobertos com tela de tule, e uma abertura superior, atrav?s da qual foi oferecido o sangue contido em uma c?lula montada em l?mina de microscopia com cola de silicone e membrana de silicone. O sangue foi mantido aquecido a 37,5 ? 0,5 ?C, durante o tempo de alimenta??o que variou de 20 a 30 minutos. O experimento foi conduzido dentro de uma c?mara climatizada, com temperatura de 28 ? 0,5 ?C, umidade relativa de 80 ? 5% e fotoperiodismo de 12 horas di?rias. Um total de 16 grupos de 30 f?meas de A. aegypti, com idades entre 4 a 7 dias, foram pr?-alimentados com solu??o a?ucarada de sacarose. Em cada grupo foram adicionados 10 machos para realiza??o de c?pula. O sangue de cada hospedeiro foi coletado assepticamente e adicionado de citrato de s?dio a 3,9%. O grupo controle foi alimentado diretamente em hospedeiro humano volunt?rio. Ap?s a alimenta??o, as f?meas ingurgitadas foram contadas e colocadas no interior de gaiolas com 50 x 50 x 50 cm, contendo frasco com ?gua limpa para oviposi??o. Foram registrados o n?mero de f?meas alimentadas em cada tratamento, o per?odo de pr?-postura e o n?mero m?dio de ovos por f?mea alimentada. Mosquitos alimentados com sangue de camundongos e de bovino tiveram performances de 22,5 e 25,8% de ingurgitamento e a postura foi em m?dia de 9,2 e 10,3 ovos por f?mea, respectivamente. F?meas alimentadas com sangue humano atrav?s da membrana ou diretamente no hospedeiro humano tiveram comportamentos diferentes com ?ndices de ingurgitamento de 89 e 95% e oviposi??o de, em m?dia, 13,4 e 15,3 ovos por f?mea, respectivamente. N?o houve varia??o no per?odo de pr?-postura (tr?s dias) entre os tratamentos realizados.

Orientador: Fausto Viterbo / Resumo: O estudo avaliou o efeito da membrana de silicone no tratamento de área cruenta de ferimento. Método: O estudo experimental foi realizado com 30 ratos Wistar, machos, divididos igualmente em três grupos: no grupo GG a área cruenta foi tratada com gazes, no grupo GH com homoenxerto e no grupo GS com membrana de silicone. Após quatorze dias de pós-operatório, os animais foram sacrificados. Os atributos estudados foram a variação da massa corporal dos animais; o estudo histológico quantificado por análise morfométrica avaliando o número de neovasos, fibroblastos, fibras colágenas, leucócitos, monócitos; e a medida da espessura da área cruenta através de régua micrométrica. Os dados foram submetidos à análise estatística. Resultados: Não houve diferença significativa entre as massas dos animais. Observou-se um predomínio de neovasos, fibroblastos e a espessura de área cruenta nos animais do grupo GS. Houve predomínio de leucócitos e monócitos nos animais do grupo GH. Também foi observado que não houve diferença significativa entre os grupos quanto às fibras colágenas. Conclusão: A membrana de silicone proporcionou tecido de granulação com maior número de neovasos, fibroblastos e maior espessura. / Abstract: The purpose of this study was to evaluate effects of the silicone membrane on treatment of wound healing base. Methods: The experimental study was with thirty Wistar male rats equally divided into three groups. Group GG the would healing base was treatment with rayon gauz, group GH with homograft and group GS with silicone membrane. The animals were sacrificed 14 postoperative day. This study evaluated weigh of rats; histological and morphometric analysis of the number of neovessels, fibroblasts, collagen fibers, leucocytes and monocytes; and measurement of wound healing base thickness was done with micrometer straight edge . The data were submitted to statistical analysis. Results: The weight of rats was similar among groups. The analysis showed considerable increased number of neovessels, fibroblasts and the measurement of would healing base thickness on the group GS; considerable increased number of leucocytes and height increased number of monocytes on the group GH. Similar pattern was observed in number of collagen fibers among three groups. Conclusion: The silicone membrane supportered wound healing base with considerable increased number of neovessels, fibroblasts and enlarging measurement of thickness. / Mestre

Made available in DSpace on 2014-06-11T19:22:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2005Bitstream added on 2014-06-13T20:09:57Z : No. of bitstreams: 1 mendesjunior_ecs_me_botfm.pdf: 5579459 bytes, checksum: 8669f77056518f9187d25fceedc26900 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O estudo avaliou o efeito da membrana de silicone no tratamento de área cruenta de ferimento. Método: O estudo experimental foi realizado com 30 ratos Wistar, machos, divididos igualmente em três grupos: no grupo GG a área cruenta foi tratada com gazes, no grupo GH com homoenxerto e no grupo GS com membrana de silicone. Após quatorze dias de pós-operatório, os animais foram sacrificados. Os atributos estudados foram a variação da massa corporal dos animais; o estudo histológico quantificado por análise morfométrica avaliando o número de neovasos, fibroblastos, fibras colágenas, leucócitos, monócitos; e a medida da espessura da área cruenta através de régua micrométrica. Os dados foram submetidos à análise estatística. Resultados: Não houve diferença significativa entre as massas dos animais. Observou-se um predomínio de neovasos, fibroblastos e a espessura de área cruenta nos animais do grupo GS. Houve predomínio de leucócitos e monócitos nos animais do grupo GH. Também foi observado que não houve diferença significativa entre os grupos quanto às fibras colágenas. Conclusão: A membrana de silicone proporcionou tecido de granulação com maior número de neovasos, fibroblastos e maior espessura. / The purpose of this study was to evaluate effects of the silicone membrane on treatment of wound healing base. Methods: The experimental study was with thirty Wistar male rats equally divided into three groups. Group GG the would healing base was treatment with rayon gauz, group GH with homograft and group GS with silicone membrane. The animals were sacrificed 14 postoperative day. This study evaluated weigh of rats; histological and morphometric analysis of the number of neovessels, fibroblasts, collagen fibers, leucocytes and monocytes; and measurement of wound healing base thickness was done with micrometer straight edge . The data were submitted to statistical analysis. Results: The weight of rats was similar among groups. The analysis showed considerable increased number of neovessels, fibroblasts and the measurement of would healing base thickness on the group GS; considerable increased number of leucocytes and height increased number of monocytes on the group GH. Similar pattern was observed in number of collagen fibers among three groups. Conclusion: The silicone membrane supportered wound healing base with considerable increased number of neovessels, fibroblasts and enlarging measurement of thickness.

A spin-cast silicone membrane has been successfully bonded between two injection-molded microstructured plastic discs. This sandwich structure creates a useful platform for mass production of microfluidic systems, provided that the bonds are leakproof. The bonds were achieved by a silicon dioxide coating deposited on the plastic discs by evaporation. This investigation is concerned with the process and the result only, no theory is discussed.