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Implementation of a Genome-Wide Survey of Induced Mutations to Identify Agronomically Valuable Variants in Chenopodium quinoaParker, Andrew Alarcon 12 April 2022 (has links)
Quinoa has been utilized for millennia in the Andes region of South America as a nutritious and hardy food crop. In recent years interest in quinoa has grown as need increases for an alternative to traditional cereal crops that can tolerate marginal environments while offering superior nutrition. Growers outside the Andes have experienced several complications adopting quinoa, including undesirable secondary metabolites, poor yield, lodging, and height inconsistency. Unfortunately, access to native ecotypes for crop improvement is limited, and desirable traits are difficult to introduce into available quinoa cultivars because of its allotetraploid genome and tendency to self-pollinate. A genome-wide survey of induced mutations in 244 sequenced M2 families was created from a bank of EMS-treated quinoa seeds and assembled into a library of mutant lineages with predicted variants and their effects on genes to assist in identifying agronomically valuable mutations in target genes as a supplement to crop improvement efforts. Using this library, eight families containing mutations in genes associated with reduced height "GAI1, GA20OX, GID1, and L " were identified. Several individuals exhibited a shorter than average phenotype; however, because each family contains thousands of EMS-induced mutations, the causative mutation of the reduced height phenotype in each family could not be definitively identified. In one family, absence of the GAI1 mutant allele, but the presence of a mutant CKX3 allele, provided a correlation between a mutation and the short phenotype. Genotyping each generation would be required for a targeted mutant allele to be tracked through selection.
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Phylogenetic Relationships of Silene sect. Melandrium and Allied Taxa (Caryophyllaceae), as Deduced from Multiple Gene TreesRautenberg, Anja January 2009 (has links)
This thesis focuses on phylogenetic relationships among some of the major lineages in Silene subgenus Behenantha (Caryophyllaceae) using DNA sequences from multiple, potentially unlinked gene regions from a large taxonomic and geographic sample. Both traditional phylogenetic analyses and a strategy to infer species trees and gene trees in a joint approach are used. A new strategy to optimize species classifications, based on the likelihoods of the observed gene trees, is presented. Silene latifolia, S. dioica and the other dioecious species previously classified in section Elisanthe are not closely related to the type of the section (S. noctiflora). The correct name for the group of dioecious species is section Melandrium. The chloroplast DNA data presented indicate a geographic, rather than a taxonomic, structure in section Melandrium. The nuclear genes investigated correlate more to the current taxonomy, although hybridization has likely been influencing the relationships within section Melandrium. Incongruence between different parts of the gene SlXY1 in two Silene lineages is investigated, using phylogenetic methods and a novel probabilistic, multiple primer-pair PCR approach. The incongruence is best explained by ancient hybridization and recombination events. A survey of mitochondrial substitution rate variation in Sileneae is presented. Silene section Conoimorpha, S. noctiflora and the closely related S. turkestanica have elevated synonymous substitution rates in the mitochondrial genes investigated. Morphological and phylogenetic data reject that the Californian S. multinervia should be treated as a synonym to the Asian S. coniflora, as has previously been suggested. Furthermore, none of the genes investigated, or a chromosome count, support the inclusion of S. multinervia in section Conoimorpha. Data from multiple genes suggest that S. noctiflora and S. turkestanica form a sister group to section Conoimorpha. The calyx nervature, which is a potential synapomorphy for S. multinervia and section Conoimorpha, may be explained either by parallelism or by sorting effects.
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