Thesis (M.Sc.)--Memorial University of Newfoundland, 1997. / Bibliography: leaves 69-79.

No description available.

In order to study sperm competition and mating dynamics in the snow crab Chionoecetes opilio, a genomic library was established with the goal of identifying highly polymorphic microsatellite markers. Six pairs of DNA primers were designed to amplify markers Cop3-4, Cop4-1, Cop5, Cop10, Cop24-3 and Cop111 by the polymerase chain reaction (PCR). All markers produced patterns as expected from single loci inherited in a mendelian fashion, except for Cop5 which revealed a multi-locus banding pattern. The cross-amplification of the six loci in seven additional crabs species revealed DNA polymorphisms at one or more loci for each species. Markers Cop3-4 and Cop24-3 were used to determine paternity of larvae of primiparous females both from the wild and from multiple mating experiments under laboratory settings. The two markers were also used to genotype the contents of female spermathecae in order to determine the number of number of male genotypes present. Spermathecal contents of wild-caught females were cut into several cross-sections and each section genotyped individually. Histological analysis of spermathecae was carried out to complement genetic data in order to elucidate patterns of sperm competition. Single paternity was observed for the progeny of all females. The analysis of laboratory females showed displacement was the mechanism by which single paternity was obtained by the last males to mate. The analysis of wild females revealed that their spermathecae contained on average the sperm of at least 3.7 males. Larvae appeared to be sired by males whose genotypes were found in the spermathecal cross-sections toward the blind-end of the spermathecae. This suggested that they were the first males to mate with females they guarded until oviposition, and females remated with other males thereafter. Also, a comprehensive account of the mating dynamics was carried out in a wild population of the Northwest Gulf of Saint Lawrence (Eastern Canada) and demonstrated the e

Thesis (M.M.S.)--Memorial University of Newfoundland, 2005. / Bibliography: leaves 85-91.

Thesis (M.Sc.)--Memorial University of Newfoundland, 2003. / Bibliography: leaves 59-61. Also available online.

This thesis investigates the influence of predator movements upon habitat selection and foraging success. It deals with two very distinct datasets one from a marine system, the snow crab (Chionoecetes opilio) fishery, and the second from a freshwater system, an experimental rainbow trout (Oncorhynchus mykiss) aquaculture operation. Deriving a standardized measure of catch from logbook data is important because catch per unit effort (CPUE) is used in fisheries analysis to estimate abundance, but it some cases CPUE is a biased estimate. For the snow crab fishery, a relative abundance measure was developed using fisher movements and logbook data that reflected commercially available biomass and produced an improved relative abundance estimate. Results from the aquaculture dataset indicate that escaped farmed rainbow trout continue to use the cage site when waste feed is available, while native lake trout do not interact with the cage. Once access to waste feed is removed, both lake trout and escaped rainbow trout do not use the cage site. This thesis uses methods to identify patterns and behaviours using movement tracks to increase our understanding of predator habitat usage.

This thesis investigates the influence of predator movements upon habitat selection and foraging success. It deals with two very distinct datasets one from a marine system, the snow crab (Chionoecetes opilio) fishery, and the second from a freshwater system, an experimental rainbow trout (Oncorhynchus mykiss) aquaculture operation. Deriving a standardized measure of catch from logbook data is important because catch per unit effort (CPUE) is used in fisheries analysis to estimate abundance, but it some cases CPUE is a biased estimate. For the snow crab fishery, a relative abundance measure was developed using fisher movements and logbook data that reflected commercially available biomass and produced an improved relative abundance estimate. Results from the aquaculture dataset indicate that escaped farmed rainbow trout continue to use the cage site when waste feed is available, while native lake trout do not interact with the cage. Once access to waste feed is removed, both lake trout and escaped rainbow trout do not use the cage site. This thesis uses methods to identify patterns and behaviours using movement tracks to increase our understanding of predator habitat usage.

Thesis (M.Eng.)--Memorial University of Newfoundland, 2004. / Bibliography: leaves 208-213.

In this thesis I assessed the causes of long-term changes in two large, commercially important decapod crustacean populations, American lobster (Homarus americanus) and snow crab (Chionoecetes opilio), in the northwest (NW) Atlantic Ocean. By combining available time-series data, including commercial landings, research surveys, and local ecological knowledge (LEK), I explored the causes of an observed ecosystem shift in the NW Atlantic (~1950–2009) which entailed a region-wide decline of groundfish and an increase in benthic invertebrates, including these decapods. Three hypotheses were examined to explain the increase in decapod abundance: (1) the predation hypothesis, whereby a decrease in predatory groundfish led to an increase in their decapod prey (top-down effects); (2) the climate hypothesis, whereby changes in temperature or other climatic variables helped to increase decapod numbers (bottom-up effects); and (3) the anthropogenic hypothesis, whereby changes in fishing pressure drove decapod population dynamics. I explored these hypotheses separately for lobster and snow crab, which may experience different ecological and commercial pressures. First, I investigated the interactions between predatory groundfish and lobster in the inshore region of southwest Nova Scotia. Long-term fisheries-independent abundance indices for lobsters and their predators are available for Gulf of Maine (GOM) waters in the USA, but not in Canada. To address research gaps I designed and executed a survey to collect the LEK of lobster fishermen fishing in the Canadian GOM. Forty-two fishermen were interviewed. Corresponding survey results from the USA were compared to the LEK results. Both sources provided evidence for a top-down effect (predation release), contributing to observed increases in GOM lobster abundance and landings. Second, I explored relationships between lobster abundance and landings in the NW Atlantic as they may relate to temporal changes in predators, temperature, climate (North Atlantic Oscillation Index, NAOI), and fishing. Available landings data and fisheries-independent abundance estimates were collated to investigate trends in lobster abundance and catch. Links between lobster, groundfish, temperature and climate indices were explored using mixed effects models. Results offered partial support for the predation hypothesis, namely in the waters off Newfoundland, Nova Scotia, and southern New England as well as broad support for a climate effect on early life stages. This effect appeared related to a region-wide climate signal, the NAOI, but was independent of changes in water temperature. Fishing effort appeared to be following lobster abundance, rather than regulating abundance in a consistent way. Third, variation in snow crab abundance was examined through meta-analysis of time-series data of cod and crab abundance and temperature. Temperature had opposing effects on the two species: snow crab abundance was negatively correlated with temperature whereas cod and temperature were positively related. Controlling for the effect of temperature, the analysis revealed significant negative interactions between snow crab and cod abundance, with cod leading snow crab up to a five-year lag. Results indicate that snow crab is largely influenced by temperature during early post-settlement years and becomes increasingly regulated by top-down mechanisms as they approach fishery recruitment. Overall, I conclude that both climate and predation can act as population controls on large decapod populations, but these variables affect decapods at different life stages.

<p dir="ltr">Seafood plays a pivotal role in global economies, livelihoods, and nutritional security. However, climate change and global pandemics pose significant threats to seafood harvests, production, supply chains, and marketing channels. The focus of my thesis is to understand the impact of external factors on our seafood resources and explore adaptive strategies in the face of uncertainties. We utilize economics techniques to study human-nature systems by zooming into social elements (government agencies, industry stakeholders, and fish farmers/fishermen) and aquatic resources. The three essays of my thesis delve into this inquiry from the perspectives of government, industry, and market, accordingly.</p><p dir="ltr">The first chapter in my thesis, <i>Climate Change and Snow Crab Harvest - Applying Random Effect Estimators with Instrumental Variable</i>, estimates the snow crab harvest function with unbalanced panel data of eastern Bering Sea snow crab, Canadian snow crab, Japanese snow crab, and Barents Sea snow crab. Specifically, we analyze the relationship between snow crab biomass, stock, and catch. To address the endogeneity of stock in the harvest function, climate change indicators are selected as instrumental variables. We identify that the Arctic Sea ice extent is effective in addressing the endogeneity and the random effects instrumental variable model with error components two stage least squares estimator performs the best to control heterogeneity. We find that a 1% increase in snow crab fishing effort is associated with a 0.42% increase in snow crab harvest, and a 1% increase in snow crab stock causes a 0.98% increase in snow crab harvest. The reported estimates indicate a large stock-harvest elasticity and provide supporting evidence for government fishery agencies to prioritize stock enhancement in policy designs.</p><p dir="ltr">The second chapter, <i>Online Media Sentiment Analysis of Shrimp and Salmon in the United States</i>, employs online media analytics on shrimp and salmon in the US to provide insights into consumer perceptions and potential demand signals for seafood. Search hits and mentions are quantified for top sources, domains, and prevalent terms. In addition, sentiment drivers and sentiment values are identified and calculated using natural language processing tools. The results reveal that the occurrence of peak mentions does not necessarily coincide with the peak of net sentiment, and farmed seafood consistently exhibits lower net sentiments compared to their wild counterparts. Autoregressive modeling is conducted to predict the dynamics of seafood’s net sentiments. The regional analysis demonstrates that public attitudes toward both farmed shrimp and salmon in the East North Central region exhibit a more positive net sentiment, while the New England and Middle Atlantic regions tend to have a lower net sentiment for farmed shrimp and salmon, respectively. The fitted forecast model serves as a supplementary tool for industry stakeholders to quickly respond to future public perceptions. Regional statistics also help the seafood industry tailor business strategies to different regions.</p><p dir="ltr">In the third chapter, <i>Comparative Case Study of Small-Scale Fish Processing for Local Seafood Supply</i><i>,</i> we examine the feasibility of utilizing a shared-use commercial kitchen and on-farm kitchen to support small-scale local fish processing, which helps diversify marketing channels in the US Midwest and supply seafood to local food systems. A case study of each facility type is assessed for economic viability for fish farmers. The financial analysis suggests farmers interested in processing tilapia or rainbow trout from 2,500 lbs to 5,000 lbs per year utilize rental commercial kitchens. A minimum of 15% markup and processing of 10,000 lbs/year tilapia is required to make the on-farm kitchen option more viable. For farmers who process rainbow trout, 10,000 lbs/year with a 10% markup using an on-farm kitchen is a better choice. Factoring in the stochastic variability of raw product prices, rental rates, and set-up costs, we provide simulated ranges for economic metrics including profitability index, payback period, and net present values. The reports of estimated costs, revenues, and breakeven prices, provide fish farmers with suggested selling prices, kitchen choices, and production levels to achieve optimum profits under risks.</p>