Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves 68-82).

Thesis (Ph. D.)--University of Missouri--Columbia, 2001. / Typescript. Vita. Includes bibliographical references (leaves 168-199). Also available on the Internet.

Thesis (Ph.D.)--University of Alabama at Birmingham, 2007. / Title from PDF title page (viewed on Feb. 19, 2010). Includes bibliographical references.

Imaging tools in cellular and molecular biology have long relied on organic fluorophores to observe microorganisms or various cell constituents. The advent of semiconductor nanoparticles known as quantum dots (QDs) has offered the possibility to use this new class of fluorescent probes with very advantageous optical properties in cell biology. The imaging of transmembrane potential and ionic currents is of significant importance for monitoring the activity of the cell. It remains possible with relatively complicated instruments and methods such as patch clamping. A complementary approach to view the dynamics of ion channels with modern and efficient fluorophores is therefore of great interest to the field of biology in general. / We developed a construct based on the FRET signal between QDs and organic fluorescent dyes to monitor the conformational changes of voltage gated sodium channels. The amino acid histidine was used as a "landing platform" for QDs and the bacterial sodium channel NaChBac was chosen for testing. This study focused on the preliminary steps of the project and aimed to characterize the electrophysiological behavior of the histidine-tagged channel. The whole-cell configuration of patch clamping was the tool we used to understand the differences between the wild-type and the histidine-tagged variants of the channels. We also explore the possibility to land QDs on the histidine tag.

Dissertation (Ph.D.)--University of South Florida, 2005. / Includes vita. Includes bibliographical references. Also available online as a PDF document.

Dissertation (Ph.D.)--University of South Florida, 2008. / Title from PDF of title page. Document formatted into pages; contains 140 pages. Includes vita. Includes bibliographical references.

Thesis (Ph.D. in Physiology & Biophysics) -- University of Colorado Denver, 2007. / Typescript. Includes bibliographical references (leaves 125-138). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;

Thesis (Ph.D.)--Indiana University, 2010. / Title from screen (viewed on April 1, 2010). Department of Pharmacology and Toxicology, Indiana University-Purdue University Indianapolis (IUPUI). Advisor(s): Theodore R. Cummins, Grant D. Nicol, Gerry S. Oxford, Andy Hudmon, John H. Schild. Includes vitae. Includes bibliographical references (leaves 232-266).

Thesis (Ph. D.)--University of Washington, 2007. / Vita. Includes bibliographical references (leaves 81-97).

Orientadores: Maria Alice da Cruz-Hofling, Anibal Eugenio Vercesi / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-06T03:04:12Z (GMT). No. of bitstreams: 1 Freitas_ErikaMariaSilva_D.pdf: 24578708 bytes, checksum: bf9367ecdbc315a07f58411feea40089 (MD5) Previous issue date: 2006 / Resumo: A veratrina, uma mistura de alcalóides obtidos da espécie de planta Shoenocaulon officinale, é uma toxina lipossolúvel que possui como sítio de ação primária os canais de sódiodependentes de voltagem. Recentemente, nossos estudos mostraram que a veratrina pode causar mionecrose e as evidências sugerem que as mitocôndrias e o retículo sarcoplasmático (RS) possam ser os alvos intracelulares da ação da veratrina. O objetivo deste trabalho foi investigar os efeitos tóxicos induzidos por diferentes concentrações de veratrina sobre o consumo de oxigênio, a atividade dos complexos da cadeia respiratória e a ultraestrutura das mitocôndrias através de análises bioquímicas, citoquímicas e morfométricas e de microscopia eletrônica de transmissão...Observação: O resumo, na íntegra, poderá ser visualizado no texto completo da tese digital / Abstract: The veratrine, a mixture of alkaloids obtained from plant Schoenocaulon officinale, is a lipid soluble toxin, which target voltage-gated Na+ channels for their primary action site. Recently, our studies showed that the veratrine may cause myonecrosis and evidences suggested mitochondria and sarcoplasmic reticuIum (SR) as intracellular cell targets. The aim of this work was to investigate the effects caused by variabIe concentration of veratrine on mitochondrial oxygen consumption, respiratory chain enzymes activities and ultrastructure, combining electron microscopy with biochemical, cytochemical and morphometrical analysis. Moreover, we investigated whether the interference on physiology of sodium channels by veratrine it alters the ultrastructure of SR on different types of muscles. Ultrastructural changes were observed on skeletal muscle mitochondria after intramuscular injection or incubation with veratrine...Note: The complete abstract is available with the full electronic digital thesis or dissertations / Doutorado / Biologia Celular / Doutor em Biologia Celular e Estrutural