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A METHOD OF DETECTING TOTAL VIABLE ORGANISMS IN WATER BASED ON SOLID-PHASE MICROEXTRACTION AND FLUORESCENT SIGNAL DETECTIONZhang, MORU 02 August 2013 (has links)
Microorganisms are monitored as a key indicator of water quality. Measurement of “total viable organisms” (TVO, similar to “heterotrophic plate count”, HPC) is used to indicate the general hygiene level of the water, and is often used to assess water treatment. Automated, instrumental TVO detection in water samples using an enzyme reaction method shortens the detection time compared to conventional HPC methods. A method that can detect TVO without interference in water samples by monitoring enzyme activities through fluorescent signals in small custom sample cells was developed.
In this method, fluorogenic substrates were cleaved by specific enzymes to make fluorescent products, which then partitioned into a non-polar siloxane polymer film on the bottom of the sample cell. A miniature spectrometer monitored fluorescence in the polymer to give a present/absent result for bacteria. There is no interference from sample color and turbidity because the light never passes through the sample matrix.
Eight substrates that can be converted by six different enzymes were characterized. These had fluorescent products coupled to glucose, glucuronic acid, galactose, phosphate, sulfate and alanine. Four different products were produced, providing detection of some enzymes simultaneously but independently, depending on the substrates chosen. Candidate fluorescent products were first tested with different siloxane polymers and two dimethyl-siloxane polymers were identified to detect all the products separately.
Fifteen laboratory bacteria strains (including E. coli, Klebsiella pneumonia, and Pseudomonas aeruginosa) were added individually to a substrate solution containing minimal nutrients and incubated. All bacteria types were successfully detected by at least one substrate, with most detected by several substrates.
Sterility of water samples from lake water, treated lake water and tap water were tested with those eight substrates separately or in combination. The lake water had the highest bacteria level and included coliforms and possibly E. coli. Treated lake water and tap water were E. coli and coliforms free, and were safe for drinking. Treated lake water had higher TVO levels than tap water, indicating a lower hygiene level. The results of combined substrate tests matched the corresponding single substrate tests, suggesting specific bacteria strains can be distinguished in a single test. / Thesis (Master, Chemistry) -- Queen's University, 2013-08-01 18:03:54.111
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TUNABLE AND HIGH REFRACTIVE INDEX POLYDIMETHYLSILOXANE POLYMERS FOR LABEL-FREE OPTICAL SENSINGLittle, JESSAMYN 26 August 2013 (has links)
There is a need for chemical sensors for monitoring volatile organic compounds (VOCs) in air. Acute and chronic inhalation of toxic VOCs can cause adverse health effects in humans, so monitoring these analytes is important for ensuring that their concentrations are maintained below maximum permissible levels. Chemical sensors using polydimethylsiloxane (PDMS) to extract VOCs with partial selectivity, coupled with label-free optical detection methods based on refractive index, can overcome the limitations of conventional VOC detection methods. A variety of tunable and high refractive index PDMS materials were developed by incorporating a range of titanium and zirconium concentrations (2.5 – 30 mol % and 2.5 – 15 mol %, respectively) using a simple sol-gel synthesis and by incorporating a range of titanium concentrations (2.5 – 10 mol %) into naphthyl-functionalized PDMS. These materials ranged in refractive index from 1.4023 ± 0.0002 to 1.5663 ± 0.0001 at 635 nm and 1.3942 ± 0.0003 to 1.5510 ± 0.0007 at 1550 nm. The ability to use tunable refractive index PDMS films to differentiate between m-xylene and cyclohexane was demonstrated by monitoring changes in refractive index and thickness following absorption of these analytes using a refractometer at 1550 nm. The sensitivity of the refractive index response to an analyte using a particular PDMS film was dependent upon the difference between the refractive index of the analyte and film, as well as the film-air partition coefficient of the analyte. The detection limits for m-xylene and cyclohexane were 81 ppm and 4940 ppm, respectively, using PDMS-titanium-oxo nanocomposites with 5 and 10 mol % Ti, respectively. A simple planar waveguide sensor with an input grating coupler was developed to monitor changes in refractive index of the cladding through shifts in peak resonance wavelength. Using high refractive index PDMS materials as the waveguide core, we monitored changes in refractive index arising from absorption of VOCs into the grating. Here, the sensitivity of the waveguide response was dependent upon the difference in refractive index of the analyte and polymer, as well as the film-air partition coefficient of the analyte. The detection limits for m-xylene and cyclohexane were 1980 ppm and 18000 ppm, respectively. / Thesis (Master, Chemistry) -- Queen's University, 2013-08-24 11:45:57.642
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Laser Desorption Solid Phase MicroextractionWang, Yan January 2006 (has links)
The use of laser desorption as a sample introduction method for solid phase microextraction (SPME) has been investigated in this research project. Three different types of analytical instruments, mass spectrometry (MS), ion mobility spectrometry (IMS) and gas chromatography (GC) were employed as detectors. The coupling of laser desorption SPME to these three instruments was constructed and described in here. <br /><br /> Solid phase microextraction/surface enhanced laser desorption ionization fibers (SPME/SELDI) were developed and have been coupled to two IMS devices. SPME/SELDI combines sampling, sample preparation and sample introduction with the ionization and desorption of the analytes. Other than being the extraction phase for the SPME fiber, the electro-conductive polymer coatings can facilitate the ionization process without the involvement of a matrix assisted laser desorption/ionization (MALDI) matrix. The performance of the SPME coatings and the experimental parameters for laser desorption SPME were investigated with the SPME/SELDI IMS devices. The new SPME/SELDI-IMS 400B device has a faster data acquisition system and a more powerful data analysis program. The optimum laser operation parameters were 250 <em>μJ</em> laser energy and 20 <em>Hz</em> repetition rate. Three new SPME coatings, polypyrrole (PPY), polythiophene (PTH) and polyaniline (PAN) were developed and evaluated by an IMS and a GC. The PPY coating was found to have the best performance and was used in most of the experiments. The characteristics of the PPY and the PTH SPME/SELDI fiber were then assessed with both IMS and MS. Good linearity could be observed between the fiber surface area and the signal intensity, and between the concentration and the signal intensities. <br /><br /> The ionization mechanism of poly(ethylene glycol) 400 (PEG) was studied with the SPME/SELDI-IMS 400B device. It was found that the potassiated ions and sodiated ions were both present in the ion mobility spectra. The results obtained with quadrupole time-of-flight (QTOF) MS confirmed the presence of both potassiated and sodiated ions. This result suggested that cationization is the main ionization process when polymers are directly ionized from the PPY coated silica surface. Four PEGs with different average molecular weights and poly(propylene glycol) 400 were also tested with this SPME/SELDI device. The differences between the ion mobility spectra of these polymers could be used for the fast identification of synthetic polymers. <br /><br /> The SPME/SELDI fibers were then coupled to QTOF MS and hybrid quadrupole linear ion trap (QqLIT) MS, respectively. Improved sensitivity could be achieved with QqLIT MS, as the modified AP MALDI source facilitated the ion transmission. The application of method for analysis of urine sample and the bovine serum albumin (BSA) digest were demonstrated with both PPY and PTH fibers. The LOD for leucine enkephalin in urine was determined to be 40 <em>fmol μL<sup>-1</sup></em> with PTH coated fiber; and the LOD for the BSA digest was 2 <em>fmol μL<sup>-1</sup></em> obtained with both PTH and PPY fibers. <br /><br /> A new multiplexed SPME/AP MALDI plate was designed and evaluated on the same QqLIT MS to improve the throughput, and the performance of this technique. The experimental parameters were optimized to obtain a significant improvement in performance. The incorporation of diluted matrix to the extraction solution improved the absolute signal and S/N ratio by 104X and 32X, respectively. The incorporation of reflection geometry for the laser illumination improved the S/N ratio by more than two orders of magnitude. The fully optimized high throughput SPME/AP MALDI configuration generated detection limit improvements on the order of 1000-7500X those achieved prior to these modifications. This system presents a possible alternative for qualitative proteomics and drug screening. <br /><br /> Laser desorption SPME as a sample introduction method for the fast analysis of non-volatile synthetic polymers was also demonstrated here. The coupling of laser desorption SPME to GC/FID and GC/MS was performed, and the advantage of laser desorption over traditional thermal desorption was demonstrated in this research. Laser desorption PEG 400 was observed more effcient than thermal desorption. Good separation was obtained even with a 1-m or 2-m column. These results demonstrate the potential of laser desorption SPME as a sample introduction method for the fast GC analysis of non-volatile compounds such as synthetic polymers.
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Novel Sputtered Stationary Phases for Solid Phase Microextraction, and Other Coatings and Materials for Surface ApplicationsDiwan, Anubhav 01 March 2016 (has links)
The primary focus of my work has been to prepare new solid adsorbents for solid phase microextraction (SPME) via sputtering of silicon. The orientation of the silica substrates/fibers and the sputtering pressure induced the formation of porous and columnar structures. Sputtering was performed for different times to yield fibers with different thicknesses. Piranha treatment of the surface increased the concentration of silanol groups, which underwent condensation with vapor deposited octadecyldimethylmonomethoxy silane to incorporate octadecyl chains onto the fiber surfaces. Silanized, sputtered fibers were preconditioned for 3 h at 320 °C to remove the unreacted chains. Comparison of the extraction efficiencies of 1.0 and 2.0 µm sputtered, silanized fibers with a commercial fiber (7 µm PDMS) for a series of analyte mixtures, which included alkanes, alcohols, aldehydes, esters, and amines, was demonstrated. The silanized, sputtered fiber performed better than the commercial fiber in extraction of most of the compounds. These fibers demonstrated long life as no degradation was seen even after 300 extractions. Carry-over between runs was not observed. The repeatability of the sputtered fibers was similar to commercial ones. The extraction of more than 50 compounds from a real world botanical sample using the 2.0 µm sputtered, silanized fiber was also demonstrated. In my second project, a facile method for the preparation of superhydrophobic surfaces (SHS) on glass and silicon surfaces was developed. A two-tier topography (needed for an SHS) was created in 60 min by the aggregation of nanosilica during in situ urea-formaldehyde polymerization. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) demonstrated rough topography. Vapor deposition of a low surface energy silane imparted hydrophobicity, which was confirmed by the presence of an F 1s signal in X-ray photoelectron spectroscopy (XPS). The prepared surfaces exhibited water contact angles (WCA) of greater than 150 °C with very low sliding angles. In my third project, a multilayer assembly of nitrilotris(methylene)triphosphonic acid, a corrosion inhibitor, and zirconium was constructed on alumina at room temperature. Attempts to prepare a layer-by-layer assembly at higher temperature (70 °C) was unsuccessful due to etching of the alumina surface. A suite of analytical techniques, XPS, AFM, time-of-flight secondary ion mass spectrometry, and spectroscopic ellipsometry was used to characterize these surfaces. This thesis also contains appendices of tutorial articles I wrote on modeling in ellipsometry, and data analysis tools (classical least squares and multivariate curve resolution).
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Impacts of Supercritical Extraction on GC/MS Profiles of Volatiles in Whey Protein Isolate Sampled by Solid Phase MicroextractionLamsen, May 01 May 2010 (has links)
Whey protein isolate (WPI) contains at least 90% protein and should ideally possess a bland flavor without typical dairy flavors including sweet aromatic and cooked/milky notes. However, its flavor may be highly variable due to factors including original whey source, processing and storage conditions. Novel technologies removing nonpolar compounds responsible for off-flavors and off-flavor formations are desirable. The major objective of this research was to evaluate impacts of supercritical carbon dioxide (scCO2) extraction, a known green process, on volatile profiles of WPI. A prior sub-objective was to establish an analytical technique for characterization of volatiles. Specifically, adsorption conditions in a well-established head-space solid-phase microextraction (SPME) method were used for quick and reliable assays of volatiles in WPI, using a divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) fiber. The adsorption of volatiles on the SPME fiber was studied at 21, 40 or 50 °C, each with durations of 5, 15 and 20 min, and analyzed by GC/MS. Based on the number of GC/MS peaks and the corresponding peak areas, adsorption conditions of 50 °C for 20 min were selected for subsequent studies. In the second sub-objective, GC/MS profiles of WPI were characterized after scCO2 extraction using a continuous stream of CO2 at 50 g/min, controlled at various combinations of temperature (30-65°C), pressure (7.0-30.0 MPa), and duration (10-90 min). Extractions with a higher temperature and a higher pressure for a longer time were generally more effective in removing volatiles, and most peaks on the chromatogram of the unprocessed WPI sample disappeared or were reduced very significantly after all studied extraction conditions, even at subcritical conditions of 7.0 MPa and 30 °C for 1 hour. Our findings demonstrated that supercritical or subcritical CO2 may provide a green approach to reduce volatiles in whey protein preparations for novel food applications.
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Detection of aldehydes in lung cancer cell culture by gas chromatography/mass spectrometry and solid-phase microextraction with on-fiber derivatizationShan, Guangqing 17 September 2007 (has links)
Aldehydes in lung cancer cell culture have been investigated using gas chromatography/mass spectrometry and solid-phase microextraction with on-fiber derivatization. In this study, the poly(dimethylsiloxane/divinylbenzene (PDMS/DVB) fiber was used and o-2,3,4,5,6-(pentafluorobenzyl) hydroxylamine hydrochloride (PFBHA) was first loaded on the fiber. Aldehydes in the headspace of lung cancer cell culture were extracted by solid-phase microextraction (SPME) fiber and subsequently derivatized by PFBHA on the fiber. Finally, the aldehyde oximes formed on the fiber were analyzed by gas chromatography/mass spectrometry (GC/MS). Using this method, acetaldehyde decrease was found in both non-small lung cancer cell cultures studied compared to the medium control study. The results of spiking the cell culture with acetaldehyde solution showed that 5 million SK-MES-1 cell lines could consume up to 4.5 uM acetaldehyde in 15-ml medium, and 5 million NCI-H522 cell lines could consume 5.9 uM acetaldehyde in 15-ml medium. The decrease of acetaldehyde may contribute to the metabolism of lung cancer cells. It was proved that GC/MS and SPME with on-fiber derivatization is a simple, rapid, sensitive and solvent-free method for the detection of aldehydes in lung cancer cell culture.
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Optimization of a Needle Trap DeviceZhan, Weiqiang 09 1900 (has links)
Various needle trap devices (NTDs) with different designs for different applications have been developed during the past decade. A theoretical model on the fundamentals of the NTD was recently proposed, which employed the theory of frontal (gas-solid) chromatography to describe the sampling process, where a gaseous sample was continuously introduced into the sorbent bed. In this investigation, different types of sorbent particles with different dimensions were packed into the needle as adsorbents. The effect of particle dimension, which would affect the packing density and consequently the capacity, the extraction efficiency, and desorption efficiency of the NTD were experimentally investigated and the proposed theory was validated. The results demonstrated that NTDs packed with small particles possess higher extraction capacity and efficiency but much higher resistance to flow as well. The higher resistance did not necessarily result in poor desorption efficiency, because desorption efficiency was affected by both the sorbent bed structure and the desorption gas flow. The relationships observed among those physical parameters provide valuable guidance on how to design an NTD with high performance potential for future applications. For particulate sampling, it was found that NTDs packed with different particles presented high collection efficiency of the particulates being investigated, and the collection efficiency was dominated by the pore size and distribution of the sorbent bed packed inside the needle. Collection efficiency also increased with increase in solidity of the sorbent bed; the increase in humidity of the aerosol sample; and the decrease of sampling rate. The results also provide valuable guidance on the optimisation of needle trap for particulate collection.
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Cold Fiber Solid Phase MicroextractionHosseinzadeh Haddadi, Shokouh January 2008 (has links)
A cold fiber solid phase microextraction device was designed and constructed based on the use of a thermoelectric cooler (TEC). A three-stage thermoelectric cooler was used for cooling a copper rod coated with a polydimethylsiloxane (PDMS) hollow fiber, which served as the SPME fiber. The copper rod was mounted on a commercial SPME plunger and exposed to the cold surface of the TEC, which was enclosed in a small aluminum box. A heat sink and a fan dissipated the generated heat at the hot side of the TEC. By applying an appropriate DC voltage to the TEC, the upper part of the copper rod, which was in contact to the cold side of the TEC, was cooled and the hollow fiber reached a lower temperature through heat transfer. A thermocouple was embedded in the cold side of the TEC for indirect measurement of the fiber temperature. A portable cold fiber SPME device was made by using a car battery as the power supply.
The cold fiber SPME device with thermoelectric cooling was applied in quantitative analysis of off-flavors in rice. Hexanal, nonanal, and undecanal were chosen as three test analytes in rice. These analytes were identified according to their retention times and analyzed with a GC/FID instrument. Headspace extraction conditions (i.e. extraction temperature and extraction time) were optimized. Standard addition calibration graphs were obtained at the optimized conditions and the concentrations of the three analytes were calculated. The developed method was compared to a conventional solvent extraction method.
The applicability of the portable cold fiber SPME with TEC for field sampling was tested. The effect of cooling on extraction recovery and the reproducibility of extraction were examined for extractions from an n-alkane flow through system. It was found that the extraction recoveries were significantly higher when the fiber was cooled. To further investigate the effect of cooling on the sensitivity of SPME in field sampling, the portable cold fiber SPME was used for extraction of volatile components from living wisteria flowers. Both the number of identified compounds and the related peak areas increased for extractions with cold PDMS fiber relative to without cooling and commercial PDMS and PA fibers. The portable cold fiber SPME device was also used for field sampling of volatile components of living lily-of-the-valley flowers and the extracted compounds were analyzed with GC/MS.
The desorption kinetics of hydrophobic organic compounds (HOCs) from environmental solid matrices was investigated using cold fiber SPME with CO2 cooling. Polycyclic aromatic hydrocarbons (PAHs) and selected volatile organic compounds (i.e. toluene, ethylbenzene, o-xylene) were used as test analytes. Sand, silica gel, and clay were used as laboratory model solid matrices and were contaminated by the test analytes. Certified sediments were used as naturally contaminated samples. In this approach, the organic compounds, released from contaminated solid samples at different elevated temperatures, were exhaustively extracted with cold fiber SPME over different extraction times. The extraction data were used to obtain desorption and Arrhenius plots. The rate constants of desorption and activation energies of desorption were measured for each contaminant using these plots. The results were comparable to those reported in the literature.
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Laser Desorption Solid Phase MicroextractionWang, Yan January 2006 (has links)
The use of laser desorption as a sample introduction method for solid phase microextraction (SPME) has been investigated in this research project. Three different types of analytical instruments, mass spectrometry (MS), ion mobility spectrometry (IMS) and gas chromatography (GC) were employed as detectors. The coupling of laser desorption SPME to these three instruments was constructed and described in here. <br /><br /> Solid phase microextraction/surface enhanced laser desorption ionization fibers (SPME/SELDI) were developed and have been coupled to two IMS devices. SPME/SELDI combines sampling, sample preparation and sample introduction with the ionization and desorption of the analytes. Other than being the extraction phase for the SPME fiber, the electro-conductive polymer coatings can facilitate the ionization process without the involvement of a matrix assisted laser desorption/ionization (MALDI) matrix. The performance of the SPME coatings and the experimental parameters for laser desorption SPME were investigated with the SPME/SELDI IMS devices. The new SPME/SELDI-IMS 400B device has a faster data acquisition system and a more powerful data analysis program. The optimum laser operation parameters were 250 <em>μJ</em> laser energy and 20 <em>Hz</em> repetition rate. Three new SPME coatings, polypyrrole (PPY), polythiophene (PTH) and polyaniline (PAN) were developed and evaluated by an IMS and a GC. The PPY coating was found to have the best performance and was used in most of the experiments. The characteristics of the PPY and the PTH SPME/SELDI fiber were then assessed with both IMS and MS. Good linearity could be observed between the fiber surface area and the signal intensity, and between the concentration and the signal intensities. <br /><br /> The ionization mechanism of poly(ethylene glycol) 400 (PEG) was studied with the SPME/SELDI-IMS 400B device. It was found that the potassiated ions and sodiated ions were both present in the ion mobility spectra. The results obtained with quadrupole time-of-flight (QTOF) MS confirmed the presence of both potassiated and sodiated ions. This result suggested that cationization is the main ionization process when polymers are directly ionized from the PPY coated silica surface. Four PEGs with different average molecular weights and poly(propylene glycol) 400 were also tested with this SPME/SELDI device. The differences between the ion mobility spectra of these polymers could be used for the fast identification of synthetic polymers. <br /><br /> The SPME/SELDI fibers were then coupled to QTOF MS and hybrid quadrupole linear ion trap (QqLIT) MS, respectively. Improved sensitivity could be achieved with QqLIT MS, as the modified AP MALDI source facilitated the ion transmission. The application of method for analysis of urine sample and the bovine serum albumin (BSA) digest were demonstrated with both PPY and PTH fibers. The LOD for leucine enkephalin in urine was determined to be 40 <em>fmol μL<sup>-1</sup></em> with PTH coated fiber; and the LOD for the BSA digest was 2 <em>fmol μL<sup>-1</sup></em> obtained with both PTH and PPY fibers. <br /><br /> A new multiplexed SPME/AP MALDI plate was designed and evaluated on the same QqLIT MS to improve the throughput, and the performance of this technique. The experimental parameters were optimized to obtain a significant improvement in performance. The incorporation of diluted matrix to the extraction solution improved the absolute signal and S/N ratio by 104X and 32X, respectively. The incorporation of reflection geometry for the laser illumination improved the S/N ratio by more than two orders of magnitude. The fully optimized high throughput SPME/AP MALDI configuration generated detection limit improvements on the order of 1000-7500X those achieved prior to these modifications. This system presents a possible alternative for qualitative proteomics and drug screening. <br /><br /> Laser desorption SPME as a sample introduction method for the fast analysis of non-volatile synthetic polymers was also demonstrated here. The coupling of laser desorption SPME to GC/FID and GC/MS was performed, and the advantage of laser desorption over traditional thermal desorption was demonstrated in this research. Laser desorption PEG 400 was observed more effcient than thermal desorption. Good separation was obtained even with a 1-m or 2-m column. These results demonstrate the potential of laser desorption SPME as a sample introduction method for the fast GC analysis of non-volatile compounds such as synthetic polymers.
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Cold Fiber Solid Phase MicroextractionHosseinzadeh Haddadi, Shokouh January 2008 (has links)
A cold fiber solid phase microextraction device was designed and constructed based on the use of a thermoelectric cooler (TEC). A three-stage thermoelectric cooler was used for cooling a copper rod coated with a polydimethylsiloxane (PDMS) hollow fiber, which served as the SPME fiber. The copper rod was mounted on a commercial SPME plunger and exposed to the cold surface of the TEC, which was enclosed in a small aluminum box. A heat sink and a fan dissipated the generated heat at the hot side of the TEC. By applying an appropriate DC voltage to the TEC, the upper part of the copper rod, which was in contact to the cold side of the TEC, was cooled and the hollow fiber reached a lower temperature through heat transfer. A thermocouple was embedded in the cold side of the TEC for indirect measurement of the fiber temperature. A portable cold fiber SPME device was made by using a car battery as the power supply.
The cold fiber SPME device with thermoelectric cooling was applied in quantitative analysis of off-flavors in rice. Hexanal, nonanal, and undecanal were chosen as three test analytes in rice. These analytes were identified according to their retention times and analyzed with a GC/FID instrument. Headspace extraction conditions (i.e. extraction temperature and extraction time) were optimized. Standard addition calibration graphs were obtained at the optimized conditions and the concentrations of the three analytes were calculated. The developed method was compared to a conventional solvent extraction method.
The applicability of the portable cold fiber SPME with TEC for field sampling was tested. The effect of cooling on extraction recovery and the reproducibility of extraction were examined for extractions from an n-alkane flow through system. It was found that the extraction recoveries were significantly higher when the fiber was cooled. To further investigate the effect of cooling on the sensitivity of SPME in field sampling, the portable cold fiber SPME was used for extraction of volatile components from living wisteria flowers. Both the number of identified compounds and the related peak areas increased for extractions with cold PDMS fiber relative to without cooling and commercial PDMS and PA fibers. The portable cold fiber SPME device was also used for field sampling of volatile components of living lily-of-the-valley flowers and the extracted compounds were analyzed with GC/MS.
The desorption kinetics of hydrophobic organic compounds (HOCs) from environmental solid matrices was investigated using cold fiber SPME with CO2 cooling. Polycyclic aromatic hydrocarbons (PAHs) and selected volatile organic compounds (i.e. toluene, ethylbenzene, o-xylene) were used as test analytes. Sand, silica gel, and clay were used as laboratory model solid matrices and were contaminated by the test analytes. Certified sediments were used as naturally contaminated samples. In this approach, the organic compounds, released from contaminated solid samples at different elevated temperatures, were exhaustively extracted with cold fiber SPME over different extraction times. The extraction data were used to obtain desorption and Arrhenius plots. The rate constants of desorption and activation energies of desorption were measured for each contaminant using these plots. The results were comparable to those reported in the literature.
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