Cette thèse porte sur la segmentation d'images OCT cutanées. Cette modalité d'imagerie permet de visualiser les structures superficielles avec une profondeur de l'ordre du millimètre. En dermatologie, elle permet d'explorer l'épiderme et sa jonction avec le derme. Cependant, les images OCT sont sévèrement affectées par le bruit speckle. Ce phénomène conjugué à la complexité inhérente aux structures de la peau rend l'interprétation des images difficile même pour des experts. L'approche classique consiste à filtrer le speckle avant de faire des traitements de segmentation. A l'inverse, dans cette thèse nous exploitons exclusivement le bruit comme information pour segmenter. Notre approche repose sur la modélisation statistique du speckle. La segmentation se fait par classification des paramètres de ce modèle probabiliste. Ainsi, - On montre que le speckle ne suit pas une loi Rayleigh, comme cela est établi analytiquement. - On ajuste plusieurs lois de probabilité à l'amplitude OCT; et on montre que celle-ci est distribuée selon la loi Gamma généralisée. - On établit que les paramètres de cette loi discriminent statistiquement les couches de la peau. - On conçoit une méthode de segmentation par classification des paramètres locaux du speckle. Les nombreuses expérimentations faites sur plusieurs corpus d'images in-vivo confirment la validité de notre approche. / This thesis deals with the segmentation of skin OCT images. This modality provides the means to visualize superficial structures down to a millimeter depth. In dermatology, it is used to examine the epidermis and its junction with the dermis. However, OCT images are severely affected by the speckle noise. This random phenomenon added to the complexity of human skin structures makes the visual interpretation of images very difficult. Classical image processing techniques filter this noise prior to any segmentation step. In this thesis, we rely exclusively on the properties of the speckle to perform segmentation. Our approach is based on the statistical modeling of the speckle. Images are segmented by classifying parameters of the statistical model. Therefore, - We show that speckle does not follow the Rayleigh distribution, as developed analytically in the literature. - We fit various probability laws to model OCT signal amplitude ; we show that Generalized Gamma has the best goodness of fit. - We establish that statistical parameters of this distribution discriminate skin layers with good variability. - We develop a segmentation method based on the classification of local statistical parameters. The various experimental results with a number of in-vivo images reported in the thesis confirm the validity of our approach

Cette thèse résulte d’une collaboration entre le Pr Ayadi de l’Institut National Polytechnique de Lorraine et le Pr Varna de l’Université Technologique de Luleå. Depuis 2005, le Professeur Varna a développé une méthode originale qui permet d’obtenir l’ensemble des constantes thermoélastiques d’un stratifié endommagé à partir de deux paramètres : l’ouverture moyenne et le glissement moyen des lèvres des fissures, normalisés par rapport à la contrainte appliquée. Dans cette thèse, nous analysons le potentiel de l’interférométrie de speckle pour obtenir la mesure de ces deux grandeurs. Plus généralement, nous montrons l’utilité de la mesure du champ de déplacement pour caractériser le développement de l’endommagement dans les stratifiés. Le premier chapitre décrit l’endommagement qui peut affecter les matériaux composites stratifiés. L’objectif est de mettre en évidence les points particuliers pour lesquels la mesure du champ de déplacement pourrait apporter des informations originales. Dans le deuxième chapitre, le principe de l’interférométrie de speckle est exposé. Nous avons particulièrement insisté sur les raisons qui nous on conduit à choisir cette technique pour réaliser cette étude. Dans le troisième chapitre, nous présentons une étude expérimentale du potentiel de l’interférométrie de speckle pour caractériser les différentes formes d’endommagement qui peuvent apparaître dans un stratifié verre/époxyde . Enfin, dans le dernier chapitre, nous analysons expérimentalement le développement de l’endommagement dans un stratifié carbone/époxyde / This work results from a collaboration between Pr Ayadi (Institut Polytechnique de Lorraine) and Pr Varna (Technical University of Luleå). Since 2005, Pr Varna has developed an original method that allows for the determination of all the thermoelastic constants of a damaged laminate. The stiffness reduction is governed by two parameters: the average value of the opening and the average value of the sliding of the crack surfaces. These values are normalized with respect to the applied loading. In this work, the potential of speckle interferometry is analysed to obtain these two parameters. More generally, we show the usefulness of displacement field measurements to characterize damage development in laminates. The first chapter is dedicated to the damage that occurs in laminates. The main objective is to highlight the points for which displacement field measurements could bring interesting information. In the second chapter, the principle of speckle interferometry is described. The choice of this technique for the considered application is discussed and justified. In the third chapter, an experimental study of the potential of speckle interferometry is proposed to characterize the damage forms that occur in a glass/epoxy laminate. In the last chapter, the development of damage is experimentally analysed in a carbon/epoxy laminate

La thématique de la thèse est très vaste. Sont présentées les grandeurs cinématiques locales ou régionales qui agissent sur le corps humain. La deuxième partie de la thèse décrit les méthodes optiques, par lesquelles on peut mesurer les vibrations produites par les machines-outils sur le système humain main-bras au cours de l'activité au travail. Dans la troisième partie sont donnés les effets négatifs des vibrations qui se produisent sur le système humain main-bras pendant l'exposition aux vibrations. La quatrième partie de cette thèse contient la base théorique des techniques optiques utilisées pour mesurer les vibrations. Le chapitre cinq présente la méthodologie d'expérimentation pour mesurer les vibrations. Les résultats des mesures sont indiqués dans le sixième chapitre de la thèse. Dans le chapitre sept on présente la modélisation mécanique et mathématique du système humain main-bras avec trois degrés de liberté et le dernier chapitre contient les conclusions finales. / In the first part of the doctoral thesis are presented the local and global kinematics sizes which are acting on the human body. This is followsed with the optical methods by which we can measure the vibrations produced by the machine-tools and them action on the human hand-arm system during the work activity. In the third part are given the effects of vibrations that occur on the hand-arm system during the exposure of vibrations. The fourth part of this thesis contains the theorical basis of optical techniques used for vibrations measurement. Chapter five presents the experimental methodology of vibrations measurement in comparition betsween the two methods: classical and optical. The results of measurement are given in chapter six of the thesis. In chapter seven is given the mechanical and mathematical modeling of human hand-arm system with three degrees of freedom and the eighth chapter contains the final conclusions, the personal contributions and the future research directions.

Cette thèse s'inscrit dans le contexte de l'amélioration de la caractérisation de l'occupation du sol à partir d'observations de télédétection de natures très différentes : le radar polarimétrique et les images optiques multispectrales. Le radar polarimétrique permet la détermination de mécanismes de rétrodiffusion provenant de théorèmes de décomposition de l'information polarimétrique utiles à la classification des types d'occupation du sol. Cependant ces décompositions sont peu compréhensibles lorsque que plu- sieurs classes thématiques co-existent dans des proportions très variables au sein des cellules de résolution radar. Le problème est d'autant plus important que le speckle inhérent à l'imagerie radar nécessite l'estimation de ces paramètres sur des voisinages locaux. Nous nous interrogeons alors sur la capacité des données optiques multispectrales sensiblement plus résolues spatialement que le radar polarimétrique à améliorer la compréhension des mécanismes radar. Pour répondre à cette question, nous mettons en place une méthode de démélange des images radar polarimétrique par séparation thématique de sources. L'image optique peut être considérée comme un paramètre de réglage du radar fournissant une vue du mélange. L'idée générale est donc de commencer par un démélange thématique (décomposer l'information radar sur les types d'occupation du sol) avant de réaliser les décompositions polarimétriques (identifier des mécanismes de rétrodiffusion).Dans ce travail nous proposons d'utiliser un modèle linéaire et présentons un algorithme pour réaliser le démélange thématique. Nous déterminons ensuite la capacité de l'algorithme de démé- lange à reconstruire le signal radar observé. Enfin nous évaluons si l'information radar démélangée contient de l'information thématique pertinente. Cette évaluation est réalisée sur des données simulées que nous avons générées et sur des données Radarsat-2 complètement polarimétriques pour un cas d'application de mélange sol nu/forêt. Les résultats montrent que, malgré le speckle, la reconstruction est valable. Il est toujours possible d'estimer localement des bases thématiques permettant de décomposer l'information radar polarimétrique puis de reconstruire le signal observé. Cet algorithme de démélange permet aussi d'assimiler de l'information portée par les images optiques. L'évaluation de la pertinence thématique des bases de la décomposition est plus problématique. Les expériences sur des données simulées montrent que celles-ci représentent bien l'information thématique souhaitée, mais que cette bonne estimation est dépendante de la nature des types thématiques et de leurs proportions de mélange. Cette méthode nécessite donc des études complémentaires sur l'utilisation de méthodes d'estimation plus robustes aux statistiques des images radar. Son application à des images radar de longueur d'onde plus longue pourrait permettre, par exemple, une meilleure estimation du volume de végétation dans le contexte de forêts ouvertes / Land cover is a layer of information of significant interest for land management issues. In this context, combining remote sensing observations of different types is expected to produce more reliable results on land cover classification. The objective of this work is to explore the use of polarimetric radar images in association with co-registered higher resolution optical images. Extracting information from a polarimetric representation consists in decomposing it with target decomposition algorithms. Understanding these mechanisms is challenging as they are mixed inside the radar cell resolution but it is the key to producing a reliable land cover classification. The problem while using these target decomposition algorithms is that average physical parameters are obtained. As a result, each land cover type of a mixed pixel might not be well described by the average polarimetric parameters. The effect is all the more important as speckle affecting radar observations requires a local estimation of the polarimetric matrices. In this context, we chose to assess whether optical images can improve the understanding of radar images at the observation scale so as to retrieve more information. Spatial and spectral unmixing methods, traditionally designed for optical image fusion, were found to be an interesting framework. As a consequence, the idea of unmixing physical radar scattering mechanisms with the optical images is proposed. The original method developed is the decomposition of the polarimetric information, based on land cover type. This thematic decomposition is performed before applying usual target decomposition algorithms. A linear mixing model for radar images and an unmixing algorithm are proposed in this document. Having pointed out that the linear unmixing model is able to split off polarimetric information on a land cover type basis, the information contained in the unmixed matrices is evaluated. The assesment is carried out with generated simulated data and polarimetric radar images from the Radarsat-2 satellite. For this experiment, textit {Bare soil} and textit {Forested area} were considered for land cover types. It was found that despite speckle the reconstructed radar information after the unmixing is statically relevant with the observations. Moreover, the unmixing algorithm is capable of assimilating information from optical images. The question whether the unmixed radar images contain relevant thematic information is more challenging. Results on real and simulated data show that this capacity depends on the types of land cover considered and their respective proportions. Future work will be carried out to make the estimation step more robust to speckle and to test this unmixing algorithm on longer wavelength radar images. In this case, this method could be used to have a better estimation of vegetation biomass in the context of open forested areas

Neste trabalho foi desenvolvido um novo método voltado para a caracterização de lentes térmicas em materiais fotônicos, utilizados como meios ativos no desenvolvimento de lasers. Este método baseia-se em interferometria por padrão de speckle eletrônico (ESPI), utilizando dois lasers de diodo multímodo sintonizados a diferentes freqüências. Com o ajuste desta diferença, foi possível escolher uma resolução apropriada para medirmos as variações geradas no raio de curvatura da frente de onda, relacionados ao efeito térmico. Para os nossos experimentos escolhemos uma amostra vítrea de aluminato de cálcio dopado com 4% de érbio; e potências de bombeio incidentes de até 1,76 mW do laser de bombeio. Os lasers de diodo foram sintonizados para ter um intervalo de contorno por volta de 120 m. Com o aumento da potência absorvida pela amostra, observamos a diminuição da curvatura da frente de onda incidente na CCD, devido ao aumento da potência da lente térmica gerada. Através de uma análise paraxial dos feixes, foi feita uma aproximação para obtermos os valores das lentes para cada configuração, apresentando comprimentos focais de 131,39 mm a 42,76 mm. / In this work we will develop a new method focused on the caracterization of thermal lenses effect in photonic materials used as active media in lasers design. This method is based on electronic speckle pattern interferometry (ESPI) using two multimode diode lasers tuned to different frequencies. Adjusting this difference we can achieve an appropriate resolution to measure the variability generated within the curvature radius of the wavefront due to thermal lens effect. For our experiments we chose a vitreous sample of calcium aluminate doped with 4% erbium and incident pump powers ranging to 1.76mW. The diode lasers were tuned to have a contour interval of around 120m. With addition in power absorbed by the sample, we observed a decrease in the curvature radius incident on the camera due to increased power of the thermal lens generated. Through a paraxial of the wavefront, an approach was made to obtain the values of the lenses for each configuration, with focal lengths ranging from 131.39 mm to 42.76 mm.

Neste trabalho serão apresentados novos métodos baseados na geração e controle de comprimento de onda em lasers aleatórios e lasers de diodo. Na primeira parte do trabalho será demonstrado um laser aleatório com realimentação localizada em filmes em biopolímeros dopado com corante. O filme é constituído por um ácido desoxirribonucleico e cloreto de cetiltrimetilamônio (DNA-CTMA) dopado com DCM. No dispositivo proposto, a realimentação óptica para o laser aleatório é dada por centros de dispersão posicionados aleatoriamente ao longo das bordas da área ativa. Os elementos de dispersão são nanopartículas de dióxido de titânio (TiO2) ou defeitos aleatórios na interface entre o polímero ativo e ar. Diferentes espectros de emissão são observados, dependendo da geometria da área excitada. Um único ressonador aleatório com dimensões de 2.6 x 0.65 mm2 foi fabricado com emissão aleatória com realimentação obtida pela excitação do dispositivo por completo. A segunda parte deste trabalho apresenta um novo método para a geração e manipulação de franjas de contorno por meio de interferometria speckle com comprimento de onda sintética, usando um único laser de diodo com cavidade externa. A cavidade externa permite sintonizar duas emissões simultaneamente, o que por sua vez muda o intervalo entre as franjas de contorno do interferômetro, além de aumentar a estabilidade do laser. Uma análise de Fourier é proposta como alternativa para medir o comprimento de onda sintético resultante das duas emissões do laser. / In this work, a new method is presented based on generating and controlling the wavelengths emitted by diode lasers and random lasers. In the first part of this work, resonant feedback random lasing from dye-doped biopolymer films is reported, consisting of a deoxyribonucleic acid and cetyltrimethylammonium (DNA-CTMA) complex doped with DCM dye. In the device, the optical feedback for random lasing is given by scattering centers randomly positioned along the edges of the active area. Scattering elements are either titanium dioxide nanoparticles or random defects at the interface between active polymer and air. Different emission spectra are observed, depending on the geometry of the excited area. A single random resonator with dimensions of 2.6 mm x 0.65 mm is fabricated and random emission with resonant feedback is obtained by uniformly pumping the full device. The second part of this work presents a new method for generating and manipulating contour fringes produced with a single external cavity diode laser in a multi-wavelength speckle interferometer. The external cavity allows tuning two simultaneous emissions, which in turn changes the contour interval of interference fringes and also improves laser stability. A Fourier analysis is applied as an alternative method for acquiring the resulting synthetic wavelength from both emissions.

La thérapie par ultrasons focalisés de haute intensité (HIFU) est une méthode non invasive et non ionisante pour l’ablation des tumeurs solides. Cependant, le mouvement des organes intra-abdominaux, dû principalement à la respiration, empêche les ultrasons de cibler correctement la tumeur. Dans ce contexte, un système HIFU robotisé tout-en-un qui compense le mouvement en temps réel pendant le traitement par HIFU a été développé. À cet effet, un système d’asservissement ultrasonore fonctionnant à une fréquence de 20 Hz, basé sur une méthode rapide de suivi du speckle ultrasonore pour l’estimation du mouvement, a été conçu. Il utilise une séquence d’alternance imagerie/exposition HIFU, dont le rapport cyclique est de 80 %, afin d’éviter les interférences d’ondes. Le système HIFU robotisé a été testé sur un fantôme imitant les tissus soumis à des mouvements sinusoïdaux 1D et 2D. Il en résulte une réduction de mouvement de plus de 80 % en 1D pour une fréquence de 0,25 Hz et de 90 % en 2D pour une fréquence de 0,1 Hz. Toutefois, il n' a pas pu être observé un effet significatif de la compensation du mouvement sur les lésions induites par HIFU. / High Intensity Focused Ultrasound (HIFU) therapy is a non-invasive and non-ionizing method for ablation of solid tumors. However, intra-abdominal organ motion, mainly due to breathing, is a major hurdle for proper targeting of the tumor. In this context, an all-in-one HIFU robotized system with motion compensation in real-time during HIFU treatment was developed. To this aim, an ultrasound visual servoing working at a frequency of 20 Hz, relying on a fast ultrasonic speckle tracking method for motion estimation, was designed. It uses an interleaved imaging/HIFU sonication sequence, with duty cycle of 80 %, in order to avoid wave interferences. The robotized HIFU system was tested on a tissue mimicking phantom undergoing a 1D and a 2D sinusoidal motion. As a result, motion reduction of more than 80 % in 1D for a frequency of 0.25 Hz and more than 90 % in 2D for a frequency of 0.1 Hz was obtained. However, it couldn't be observed a significant effect of motion compensation on the lesions induced by HIFU.

Neste trabalho serão apresentados novos métodos baseados na geração e controle de comprimento de onda em lasers aleatórios e lasers de diodo. Na primeira parte do trabalho será demonstrado um laser aleatório com realimentação localizada em filmes em biopolímeros dopado com corante. O filme é constituído por um ácido desoxirribonucleico e cloreto de cetiltrimetilamônio (DNA-CTMA) dopado com DCM. No dispositivo proposto, a realimentação óptica para o laser aleatório é dada por centros de dispersão posicionados aleatoriamente ao longo das bordas da área ativa. Os elementos de dispersão são nanopartículas de dióxido de titânio (TiO2) ou defeitos aleatórios na interface entre o polímero ativo e ar. Diferentes espectros de emissão são observados, dependendo da geometria da área excitada. Um único ressonador aleatório com dimensões de 2.6 x 0.65 mm2 foi fabricado com emissão aleatória com realimentação obtida pela excitação do dispositivo por completo. A segunda parte deste trabalho apresenta um novo método para a geração e manipulação de franjas de contorno por meio de interferometria speckle com comprimento de onda sintética, usando um único laser de diodo com cavidade externa. A cavidade externa permite sintonizar duas emissões simultaneamente, o que por sua vez muda o intervalo entre as franjas de contorno do interferômetro, além de aumentar a estabilidade do laser. Uma análise de Fourier é proposta como alternativa para medir o comprimento de onda sintético resultante das duas emissões do laser. / In this work, a new method is presented based on generating and controlling the wavelengths emitted by diode lasers and random lasers. In the first part of this work, resonant feedback random lasing from dye-doped biopolymer films is reported, consisting of a deoxyribonucleic acid and cetyltrimethylammonium (DNA-CTMA) complex doped with DCM dye. In the device, the optical feedback for random lasing is given by scattering centers randomly positioned along the edges of the active area. Scattering elements are either titanium dioxide nanoparticles or random defects at the interface between active polymer and air. Different emission spectra are observed, depending on the geometry of the excited area. A single random resonator with dimensions of 2.6 mm x 0.65 mm is fabricated and random emission with resonant feedback is obtained by uniformly pumping the full device. The second part of this work presents a new method for generating and manipulating contour fringes produced with a single external cavity diode laser in a multi-wavelength speckle interferometer. The external cavity allows tuning two simultaneous emissions, which in turn changes the contour interval of interference fringes and also improves laser stability. A Fourier analysis is applied as an alternative method for acquiring the resulting synthetic wavelength from both emissions.

Neste trabalho foi desenvolvido um novo método voltado para a caracterização de lentes térmicas em materiais fotônicos, utilizados como meios ativos no desenvolvimento de lasers. Este método baseia-se em interferometria por padrão de speckle eletrônico (ESPI), utilizando dois lasers de diodo multímodo sintonizados a diferentes freqüências. Com o ajuste desta diferença, foi possível escolher uma resolução apropriada para medirmos as variações geradas no raio de curvatura da frente de onda, relacionados ao efeito térmico. Para os nossos experimentos escolhemos uma amostra vítrea de aluminato de cálcio dopado com 4% de érbio; e potências de bombeio incidentes de até 1,76 mW do laser de bombeio. Os lasers de diodo foram sintonizados para ter um intervalo de contorno por volta de 120 m. Com o aumento da potência absorvida pela amostra, observamos a diminuição da curvatura da frente de onda incidente na CCD, devido ao aumento da potência da lente térmica gerada. Através de uma análise paraxial dos feixes, foi feita uma aproximação para obtermos os valores das lentes para cada configuração, apresentando comprimentos focais de 131,39 mm a 42,76 mm. / In this work we will develop a new method focused on the caracterization of thermal lenses effect in photonic materials used as active media in lasers design. This method is based on electronic speckle pattern interferometry (ESPI) using two multimode diode lasers tuned to different frequencies. Adjusting this difference we can achieve an appropriate resolution to measure the variability generated within the curvature radius of the wavefront due to thermal lens effect. For our experiments we chose a vitreous sample of calcium aluminate doped with 4% erbium and incident pump powers ranging to 1.76mW. The diode lasers were tuned to have a contour interval of around 120m. With addition in power absorbed by the sample, we observed a decrease in the curvature radius incident on the camera due to increased power of the thermal lens generated. Through a paraxial of the wavefront, an approach was made to obtain the values of the lenses for each configuration, with focal lengths ranging from 131.39 mm to 42.76 mm.

Le speckle laser est un effet d'interférence longtemps considéré comme néfaste lors de l'utilisation de sources de lumière cohérente. Cependant, pour certaines applications, cet effet peut être bénéfique et utilisé comme source d'information. C’est le cas du domaine biomédical.Ainsi, le speckle laser est utilisé depuis des décennies pour la surveillance du flux sanguin microvasculaire. Il commence à être considéré aussi pour l'extraction de paramètres de la macrocirculation sanguine. Ce travail vise donc tout d’abord à démontrer que le speckle laser permet d'évaluer les paramètres hémodynamiques de la macrocirculation avec fiabilité et à partir d’une technique identique à celle employée dans l’étude de la microcirculation. Ceci conduira à une intégration rapide du dispositif dans les instruments existants. Par ailleurs, l'un des problèmes les plus importants du speckle laser,empêchant l’obtention d’une analyse totalement quantitative, est l'effet des diffuseurs statiques. Ce type de diffuseurs influence fortement le contraste de speckle, conduisant à une mauvaise interprétation des données. Le second objectif de ce travail est donc d'étudier l'effet des diffuseurs statiques sur la corrélation et le contraste du speckle laser. Nos résultats montrent tout d’abord que le speckle laser est un phénomène intéressant pour extraire les paramètres hémodynamiques de la macrocirculation. Par ailleurs, nos études révèlent que le calcul de la corrélation du speckle laser permet d'estimer le rapport entre diffuseurs statiques / dynamiques avec une bonne fiabilité. En outre, le contraste temporel permet de déterminer les diffuseurs dynamiques possédant des vitesses différentes. / The laser speckle is an interference effect that has been considered as a main drawback in the use of coherent light sources. However, for a specific set of applications, this effect can become a source of information. Among these applications there are the biomedical ones. The laser speckle has been used for decades to monitor microvascular blood flow but only now starts to be considered as a method that can also be used for macrocirculation parameters extraction. This work first aims at demonstrating that laser speckle can be used for macrocirculation assessment with good reliability, using the same technique as the one employed in microcirculation assessment. The use of the same methods could lead to a rapid inclusion of this new evaluation in the existing devices. Furthermore, one of the most important laser speckle issues, that prevents a fully quantitative analysis, is the effect of static scatterers. This type of scatterers strongly influences the speckle contrast, leading to a wrong interpretation of the data. The second objective of this work is to study the effect of statics catterers on the laser speckle correlation and contrast. Our results show that the laser speckle is an interesting phenomenon to extract hemodynamic parameters in the macrocirculation. This work also demonstrates that the laser speckle correlation is able to estimate the ratio between static/dynamic scatterers with good reliability. Moreover, the temporal speckle contrast achieved a very good performance in discerning dynamic scatterers with different velocities.