Epitachophoresis is newly developed method, which allows to separate and concentrate analytes from large volumes of complex samples. Principle is based on isotachophoresis and utilize discontinuous electrolyte system. Different design of device is used, its round shaped and due to that acceleration of analytes zone migration occurs during separation towards centre of device and at the same time widening of zones happens. Focusation, concentration and separation of analytes between leading and terminating electrolyte progress from outer line of device towards its centre. It is necessary to stabilize this boundary to prevent mixing of leading and terminating electrolyte. Main target of this diploma thesis is optimization of epitachophoretic conditions, mainly electrolyte boundary stabilization options. Tested stabilizing media were agarose gels, foamed polymer disks and PDMS columns. All leading and terminating electrolyte boundary stabilizing media undergo several optimizations so the best option can be selected for concreate applications. DNA was used as a model analyte and the media with the highest recovery were selected. The best results offered NEEO and PFEG agarose gels and Dyneema foamed polymer with recovery over 70%. Another interesting alternative are PDMS columns, with their open structure that allow concentration of large volume analytes such as microorganisms.

Role of PsbO isoforms in Arabidopsis thaliana Abstract Photosystem II (PSII) uses sunlight to catalyze water oxidation and reduce plastoquinone. Water oxidation takes place in oxygen evolving complex (OEC). OEC is stabilized by extrinsic subunits of PSII. The largest and most important of them is PsbO, manganese-stabilizing protein which can be found in all known oxygenic photosynthetic organisms. Model plant Arabidopsis thaliana expresses two isoforms of psbO gene, namely PsbO1and PsbO2.Mutants psbo1 and psbo2 lacking PsbO1 and PsbO2, respectively, recently brought new findings on the particular roles of isoforms in maintaining photosynthesis. PsbO1 is commonly considered as the main isoform facilitating water splitting, whereas PsbO2 is believed to be involved in PSII repair process (replacement of photodamaged D1 subunit). This work focuses on particular roles of Arabidopsis PsbO isoforms in maintaining photosynthesis with special focus on response to light stress. Mutants psbo1, psbo2 and wild type plants Col-0 were used for extensive biochemical investigation. Our aim was to find out what is the impact on overall thylakoid structure and composition in mutants. Furthermore, to investigate response to light stress in wild type regarding to yields of particular subcompartments, changes in photosystem II...

High resolution transient/3D imaging technology is of high interest in both scientific research and commercial application. Nowadays, all of the transient imaging methods suffer from low resolution or time consuming mechanical scanning. We proposed a new method based on TCSPC and Compressive Sensing to achieve a high resolution transient imaging with a several seconds capturing process. Picosecond laser sends a serious of equal interval pulse while synchronized SPAD camera's detecting gate window has a precise phase delay at each cycle. After capturing enough points, we are able to make up a whole signal. By inserting a DMD device into the system, we are able to modulate all the frames of data using binary random patterns to reconstruct a super resolution transient/3D image later. Because the low fill factor of SPAD sensor will make a compressive sensing scenario ill-conditioned, We designed and fabricated a diffractive microlens array. We proposed a new CS reconstruction algorithm which is able to denoise at the same time for the measurements suffering from Poisson noise. Instead of a single SPAD senor, we chose a SPAD array because it can drastically reduce the requirement for the number of measurements and its reconstruction time. Further more, it not easy to reconstruct a high resolution image with only one single sensor while for an array, it just needs to reconstruct small patches and a few measurements. In this thesis, we evaluated the reconstruction methods using both clean measurements and the version corrupted by Poisson noise. The results show how the integration over the layers influence the image quality and our algorithm works well while the measurements suffer from non-trival Poisson noise. It's a breakthrough in the areas of both transient imaging and compressive sensing.

京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第21620号 / 医博第4426号 / 新制||医||1033(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 平井 豊博, 教授 宮本 享, 教授 福田 和彦 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM

Bakgrund: Graviditetsrelaterad bäcken- och ländryggssmärta är vanligt förekommande. Smärtan försvinner oftast efter förlossning men för 7–10% kvarstår den i mer än två år. Det finns väl utformade behandlingsrekommendationer för den gravida kvinnan, men desto mindre för kvinnor med långvariga smärtbesvär. Stabiliserande träning är en typ av behandling som kan innefatta övningar för de olika muskelsystem som stabiliserar vår bål. Syfte: Att genom en strukturerad litteraturstudie undersöka vilken effekt stabiliserande träning har avseende smärta och funktion vid långvarig bäcken- och ländryggssmärta hos kvinnor efter förlossning. Metod: Datainsamlingen genomfördes i de elektroniska databaserna PubMed, CINAHL och PEDro. Urval gjordes utifrån studiens syfte, frågeställningar och uppsatta inklusions- och exklusionskriterier. De potentiellt relevanta artiklarnas metodologiska och etiska kvalitetgranskades. Data extraherades, sammanställdes i tabeller och analyserades kvalitativt. Smärta skattades genom Visual Analogue Scale (VAS) eller Numeric Pain Rating Scale (NPRS), funktion genom Oswestry Disability Index (ODI) eller Modified Oswestry Disability Questionnaire (MODQ). Resultat: Totalt inkluderades sju randomiserade kontrollerade studier (RCT) och 393 studiedeltagare. Studiekvaliteten varierade, tre studier bedömdes ha hög risk för bias, tre medelhög och en låg. Fem av sju studier uppvisade en statistiskt signifikant förbättring av utfallsmåtten smärta och/eller funktion efter avslutad intervention till förde linterventionsgruppen. Slutsats: Litteraturstudien indikerar positiva resultat för effekter av stabiliserande träning som behandling till kvinnor med långvarig bäcken- och ländryggssmärta efter förlossning, avseende smärta och funktion. För att med högre tillförlitlighet få veta vilken effekt stabiliserande träning har jämfört med andra behandlingsmetoder, samt hur vi bör utforma träningen i klinik, behövs mer forskning på området. / Background: Pregnancy related pelvic- and low back pain is common. The pain often disappears after childbirth but for 7-10% this pain remains for more than two years. There are treatment recommendations for the pregnant woman but limited for women with long lasting pain after childbirth. Stabilizing training is a treatment that includes exercises for the different muscle systems that stabilize our trunk. Aim: Through this structured literature study investigate the effects of stabilizing training, on pain and disability, for women with persistent pelvic-and low back pain after childbirth. Method: Data collection was performed in the databases Pubmed, CINAHL and PEDro. Selection was made from the aim, inclusion-and exclusion criteria of the study. An assessment of quality and ethical aspects of the articles was made. Data were extracted, compiled in tables and analyzed qualitatively. Pain was estimated by Visual Analogue Scale(VAS)/Numeric Pain Rating Scale (NPRS), disability by Oswestry Disability Index(ODI)/Modified Oswestry Disability Questionnaire (MODQ). Results: Totally seven RCT-studies and 393 study participants were included. Quality of the studies varied, three studies assessed to be high risk of bias, three medium and one low risk.Five of seven studies showed statistically significant improvement by the outcomes pain and function to advantage the intervention group. Conclusion: The review indicates positive results for the effects of stabilizing training for women with pelvic- and low back pain after childbirth. To assess the effects of stabilizing training with higher reliability, and how we should design the training in clinic, more research is needed.

Through the electrification of bicycles, the implementation of new active and passive safety systems becomes possible. Examples for such systems are bicycle ABS, TU Delft - Fall Prevention Bicycle, Bosch Help Connect (eCall System for bicycles), airbag helmets and many others. One of the main difficulties in developing and testing such safety systems is, that test riders should not be exposed to high risks when testing early prototypes. Thus, an automated or remote-controlled test platform for the analysis of bicycle dynamics and for testing of newly developed safety systems could boost the development of such systems and make it safer. The main difficulty when developing such a test platform, which has been addressed in this work, is stabilizing it at low speeds (1.5 m/s - 4.5 m/s) and being capable of tracking a desired yaw rate, only using a steer actuator. In the following, the development of such a test platform is described and first experimental results are presented.

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<p> Conventional method for testing the total power loss and internal temperature rise of induction motors under full load can often be a complex and costly process. The new phase modulation method for synthetic full-load testing of induction machines has been proven viable, provided the power oscillation in this method can be minimized. This thesis explores two techniques for stabilizing power fluctuation in the new method, and determines the test system's power sensitivities to parameter changes in the equivalent circuit of the induction motors under test.</p> <p> A computer simulation representing the test system used in the new phase modulation method was developed, and an experimental testing facility was built to test the technique devised for suppressing power oscillation in the test system.</p> <p> The results from simulations are analyzed and compared to those obtained from the actual experiments in order to identify the feasible power-suppressing technique, and determine the induction machine parameters responsible for causing power unbalance in the test system.</p> / Thesis / Master of Applied Science (MASc)

The alm of the work was to evaluate the impact of facilitation of physiologic coordination of respiratory and postural functions of diaphragm by Vojta-therapy on gas exchange. This thesis summarizes available knowledge about anatomical background of diaphragm functions, together with other trunk muscles known as deep stabilizing system. Moreover, it describes coordination of breathing and postural functions. We tried to objectify impact of diaphragm activation on gas exchange in healthy adults. Two following spiroergometries were compared aťt:er Vojta-therapy stimulation and with placebo. We evaluated minute ventilation, breath frequency, tidal volume, oxygen consumption, rate oť elimination of carbon dioxide and respiratory quotient. There were no statistical differences between placebo and Vojta- therapy group at exercise intensity levels 1 W /kg, 2 W /kg and in maximal exercise. Powered by TCPDF (www.tcpdf.org)

PsbO (manganese-stabilizing protein) is the largest extrinsic protein of photosystem II, located on the lumen side of photosystem. It is present in all known oxyphototrophic organisms. PsbO facilitates photosynthetic water splitting, which takes place in an oxygen evolving center (Mn4CaO5 cluster) of photosystem II. This work is focused on PsbO of higher plants and its isoforms, particularly their evolution and functions. Bioinformatic analyses revealed that majority of higher plants express exactly two psbO isoforms. A phylogenetic tree of PsbO sequences has an unusual topology. The two paralogous isoforms do not diverge at the base of the phylogenetic tree, as anticipated, but rather at the end of particular branches, at the level of family or lower taxonomic unit. In this work we propose and discuss several hypotheses concerning evolution of PsbO isoforms. The work further includes detailed analysis and identification of protein spots assigned to PsbO on 2D IEF-SDS PAGE gels of potato thylakoid proteins. We identified predominant version of PsbO isoform in most of the spots. We did not succeed to find any posttranslational modification. We optimized a method of psbO expression in E. coli and subsequent purification, which yielded relatively big amount of properly folded recombinant protein. Analysis of...