Salt marsh food webs are complex systems, with high levels of in situ primary production supporting a wealth of resident and migratory species. In this study, we use stable isotopes as a tool to trace organic matter utilization within the Nueces Marsh food web. Specifically, we were interested in (1) the use of treated wastewater as a ¹⁵N tracer, and (2) seasonal and interannual variation in food web structure. We hypothesized that treated wastewater would selectively label detrital resourse use within the food web, allowing us to trace grazing vs. detrital pathways within the marsh system. We also hypothesized that marsh consumers would exhibit distinct differences in isotopic composition between summer and winter, and between different years. We found that the Nueces Marsh food web consists of 3.5 consumer trophic levels. The [delta]¹³C values of consumer organisms were similar across the spatial extent of the low marsh, regardless of proximity to wastewater inflow. However, a majority of the organisms collected from the wastewater channel were significantly (p<0.05) enriched in ¹⁵N compared to their reference counterparts. We propose that ¹⁵N-enriched nitrogen is entering the Nueces Marsh food web through detrital rather than grazing-based pathways, making wastewater effluent an effective tracer of detrital integration into a marsh food web system. Hydrologic data indicate that isotopic shifts between seasons and between years reflected larger scale shifts between drought and wet years. During drought years, decreased production by phytoplankton and emergent plants led consumers to rely more heavily on ¹³C-enriched cyanobacterial carbon. In contrast, wet years encourage phytoplankton and emergent plant production, making cyanobacterial carbon relatively less exploited. While the Nueces Marsh food web is supported by a stable detrital carbon pool, it may still be susceptible to larger scale hydrologic events. / text

Bythotrephes longimanus, a predatory exotic cladoceran, has spread rapidly to numerous lakes through the Laurentian Great Lakes region of Ontario and North America. Post-invaded lakes are known to have reduced zooplankton species richness, biomass and altered community structure. Bythotrephes may also affect the diet and trophic position of macroinvertebrate predators and prey species for fish (e.g., Mysis relicta). However, the effects of this species in altering higher trophic levels remain largely unexamined. Using a combined approach of stable isotope (d13C and d15N) and THg analysis, the trophic position of Bythotrephes in two invaded lakes was investigated. Based on d15N values, Bythotrephes shared a similar trophic position to native macroinvertebrate predators (9 and 7‰ for Peninsula and Harp lakes, respectively). Using a mixing model and stomach content analysis we show that, despite low and patchy lake abundance, Bythotrephes may be a key prey item to fish and has approximate dietary contributions similar to native prey items, such as zooplankton, Chaoborus and Mysis. In both lakes, Hg conformed to predicted biomagnification trends as indicated by 15N. When Bythotrephes invades lakes with native macroinvertebrate predators, it inserts itself into the same trophic position and does not have major effects on food web length. / Thesis (Master, Biology) -- Queen's University, 2008-01-10 22:00:22.188

No description available.

This thesis investigates the relationships between mercury (Hg) and stable isotope of carbon (δ13C) in marine pelagic zooplankton (Calanus spp., Themisto spp. and Euchaeta spp.) with water mass characteristics in the North Water Polynya (NOW) and in the Mackenzie shelf – Amundsen Gulf area. Two ship based sampling field expeditions were carried out in late summer of 2005 and 2006 in both regions on board the CCGS Amundsen. In the North Water (NOW) polynya, higher levels of water Hg, depleted δ18O, lower salinity and lower nitrate levels were measured at sampling locations near the Prince of Wales glacier (POW) on the eastern coast of Ellesmere Island in the Smith Sound area. These results suggest that the glacier may be a source of Hg to this region which, in turn, is responsible for the correspondingly high concentrations of THg and MMHg measured in Calanus spp. and Euchaeta spp. at the same locations. The Mackenzie shelf – Amundsen Gulf region was characterized by fresher surface water properties (low salinity and depleted δ18O) in the western part and was strongly linked to the influence of the Mackenzie River. Higher THg concentrations in zooplankton were associated with larger fractions of both meteoric water and sea-ice melt. These findings suggest that in the western Arctic, inorganic Hg uptake in zooplankton via-absorption near surface water was highly driven by freshwater inputs into the system. Based on the analysis of three main genus Calanus spp. (mostly adult females Calanus hyperboreus), Euchaeta spp. and Themisto spp. (mostly adult Themisto libellula), THg and MMHg concentrations were the highest in the carnivorous copepod Euchaeta spp. in the North Water polynya followed by the omnivorous hyperiid amphipod Themisto spp. The herbivorous copepod Calanus spp. had both the lowest THg and MMHg concentrations in the Eastern and the Western Arctic. In addition, the Western Arctic is the area in which each zooplankton genus had the most depleted carbon and the most enriched nitrogen. The highest concentrations of THg in Calanus spp., Euchaeta spp. and Themisto spp. were measured in the Western Arctic as well as the highest MMHg in Calanus spp. and Themisto spp. The highest %MMHg was calculated in the Archipelago for Themisto spp., in the Eastern Arctic for Euchaeta spp. and in the Western Arctic for Calanus spp. The relationships observed between THg, MMHg, %MMHg and δ13C in all three major zooplankton taxa and water mass properties were in agreement with what have been previously described in the literature. Our findings suggested that both Hg and δ13C can be used as tracers to help understand zooplankton vertical distribution, feeding ecology and ultimately to predict climate changes impact at lower trophic level in the pelagic food web. The implications for marine mammals foraging in these regions are also discussed.

This thesis presents research that improves our understanding of the relationship between climate and tree-ring stable isotopes in New Zealand kauri (Agathis australis (D. Don) Lindl.) and cedar (Libocedrus bidwillii Hook. F). As a whole, this research creates a framework from which future investigations of stable isotope dendroclimatology can pursued at high temporal resolution across longer term temporal intervals. Such a framework is required to realize the full potential of New Zealand tree-ring records as archives of palaeoclimatic information. In the Southern Hemisphere, mid-to-high latitude terrestrial climate proxy records are sparse; the long-lived endemic conifers of New Zealand present a rare opportunity to gain valuable insight into Late-Quaternary and Holocene climatic change. A major barrier to long stable isotope dendroclimatic proxy records has been the time-and-resource intensive nature of associated sample preparation and analytical processes. Through experimental assessment of the SBrendel α-cellulose extraction method, this research demonstrates that this simple processing method can, with some caveats, be successfully applied to resinous conifers, facilitating rapid chemical preparation of samples. Further efficiency gains are documented in through use of dual element low temperature pyrolysis of cellulose, providing carbon and oxygen stable isotope determinations on a single sample, rather than the traditionally separate analyses. These preliminary investigations into methodological efficiency enabled the ~3000 stable isotope determinations on kauri and cedar, on which the following investigations into isotopic variability, chronology quality and climate-isotope relationships are based. Both kauri and cedar exhibit substantial intra and inter-tree isotopic variability, resulting in greater than normal sample numbers being required to isolate the common variance in composite time-series. Despite sampling 7 trees (18 cores) at one site and 8 trees (21 cores) at another, only oxygen isotope time-series reach an Expressed Population Signal score of 0.85, a common benchmark of chronology quality. Further investigations using high-resolution sequential sampling of single rings show that this variability extends to a sub-annual scale. Within single rings there are large ranges in δ13C and δ18O, generally corresponding with regular annual cycles of up to 4.6‰ for δ13C and 8.1‰ for δ18O. These data show high frequency (sub weekly) changes in δ18O, demonstrating sufficiently fine resolution for event-to-seasonal scale climate/weather reconstruction. However, that potential is complicated by the lack of strong climatic correlations with isotopic cycles. Due to their stronger common signal, δ18O time series are investigated for inter-annual stable isotope dendroclimatic reconstruction from kauri and cedar. Bootstrapped correlation and multiple regression models show strong relationships between kauri and cedar δ18O and relative humidity, accumulated rainfall and soil moisture deficit. The correlations are strongest for all variables during the period from Autumn prior to growth to the current growth summer. Tree-ring δ18O in kauri and cedar is identified as an effective recorder of regional hydroclimate leading up to and during the Austral growth season. While it is demonstrated that tree-ring δ18O holds significant promise for inter-annual palaeoclimate reconstruction, it is equally apparent from this research that stable isotope dendroclimatology in New Zealand faces significant challenges. Foremost is gaining an improved understanding of seasonal dendrochemical cycles and their relationship with tree-physiology. Increasing the spatial and temporal coverage of tree-ring isotope records is vital to realising New Zealand’s vast potential for stable isotope dendroclimatic reconstruction.

This thesis investigates the relationships between mercury (Hg) and stable isotope of carbon (δ13C) in marine pelagic zooplankton (Calanus spp., Themisto spp. and Euchaeta spp.) with water mass characteristics in the North Water Polynya (NOW) and in the Mackenzie shelf – Amundsen Gulf area. Two ship based sampling field expeditions were carried out in late summer of 2005 and 2006 in both regions on board the CCGS Amundsen. In the North Water (NOW) polynya, higher levels of water Hg, depleted δ18O, lower salinity and lower nitrate levels were measured at sampling locations near the Prince of Wales glacier (POW) on the eastern coast of Ellesmere Island in the Smith Sound area. These results suggest that the glacier may be a source of Hg to this region which, in turn, is responsible for the correspondingly high concentrations of THg and MMHg measured in Calanus spp. and Euchaeta spp. at the same locations. The Mackenzie shelf – Amundsen Gulf region was characterized by fresher surface water properties (low salinity and depleted δ18O) in the western part and was strongly linked to the influence of the Mackenzie River. Higher THg concentrations in zooplankton were associated with larger fractions of both meteoric water and sea-ice melt. These findings suggest that in the western Arctic, inorganic Hg uptake in zooplankton via-absorption near surface water was highly driven by freshwater inputs into the system. Based on the analysis of three main genus Calanus spp. (mostly adult females Calanus hyperboreus), Euchaeta spp. and Themisto spp. (mostly adult Themisto libellula), THg and MMHg concentrations were the highest in the carnivorous copepod Euchaeta spp. in the North Water polynya followed by the omnivorous hyperiid amphipod Themisto spp. The herbivorous copepod Calanus spp. had both the lowest THg and MMHg concentrations in the Eastern and the Western Arctic. In addition, the Western Arctic is the area in which each zooplankton genus had the most depleted carbon and the most enriched nitrogen. The highest concentrations of THg in Calanus spp., Euchaeta spp. and Themisto spp. were measured in the Western Arctic as well as the highest MMHg in Calanus spp. and Themisto spp. The highest %MMHg was calculated in the Archipelago for Themisto spp., in the Eastern Arctic for Euchaeta spp. and in the Western Arctic for Calanus spp. The relationships observed between THg, MMHg, %MMHg and δ13C in all three major zooplankton taxa and water mass properties were in agreement with what have been previously described in the literature. Our findings suggested that both Hg and δ13C can be used as tracers to help understand zooplankton vertical distribution, feeding ecology and ultimately to predict climate changes impact at lower trophic level in the pelagic food web. The implications for marine mammals foraging in these regions are also discussed.

Thesis (M.S.)--The University of North Carolina at Greensboro, 2010. / Directed by Anne Hershey; submitted to the Dept. of Biology.

Tomatoes (Lycopersicon esculentum L.) require high amounts of nitrogen to maximize fruit production. The type of nitrogen and timing of fertilizer applications are important in tomato production systems to reduce nitrogen losses while optimizing yields. A two-year greenhouse study was conducted to determine the effects of organic and inorganic fertilization treatments on nitrogen and carbon isotopic compositions of tomato plant leaves and fruit at immature and mature stages of plant growth. The carbon and nitrogen isotopic ratios together will also help to better depict trends that develop from conventional versus organically grown tomatoes and their resulting water use efficiency (WUE). ‘Better Bush’ tomatoes were grown in 8 L plastic pots filled with 1:1:1 steamed-sterilized sand:silt loam soil:peat mix. Eight fertility treatments were evaluated: no fertility, synthetic Miracle Grow® (MG), organic bonemeal and bloodmeal (BB), BB with liquid Earthjuice (BBL), BB with 25% vermicompost (VC), BBL with 25% VC, MG with 25% VC, and no fertility with 25% VC. The results indicated that for both growth stages, δ15NAir differed (P ≤ 0.05) between fertility treatments, while no major differences were observed for δ13CVPDB (P > 0.05), although conventionally fertilized tomatoes with MG and MG with 25% VC tended to have lower δ13CVPDB values suggesting a greater transpirational water loss through open stomata. The organic treatments with VC had higher δ15NAir values than the conventional or no added fertility treatments for all five leaflets from three different branches, fruit skins from the first two fruit clusters, fruit juices from the first two clusters and soil samples. Generally, the 15N/14N stable isotope values of tomato foliage and fruit are distinctly different between organic and conventional fertilizers, which could provide a powerful forensic tool in fingerprinting tomatoes grown by organic farming methods. Therefore, nitrogen isotopes can distinguish among different fertility treatments and also help to label the VC applications, which are thought to provide better fertility management due to less soil leaching and volatilization.

Marine mammals breeding in sympatry use resources differently depending on their time-activity budgets and prey distribution. We measured isotopic values and patterns of δ15N and δ13C in keratinous whiskers of satellite-tagged adult female Antarctic (Arctocephalus gazella) - and Subantarctic (Arctocephalus tropicalis) fur seals from three colonies at Marion Island in the Southern Indian Ocean. The three sympatric colonies were Mixed Pickle (HD_MP), Rockhopper Bay (LD_RhB), and Watertunnel (HD_WT). A comparison between two colonies of differing A. tropicalis densities will also be investigated. We investigated resource use in breeding mature females over varying time and spatial scales during summer and winter. The aim was to understand the variation in resource and habitat use at population and individual levels. The whiskers of A. gazella and A. tropicalis grow constantly at a rate of 0.16 ± 0.05 day-1 and 0.12 ± 0.05 day-1, respectively. To compare and relate behaviours, the stable isotope data and satellite tracks were grouped into winter and summer (pooled for 2010 - 2014). In summer, A. gazella from HD_WT had a larger isotopic niche width (0.85 ± 0.67‰2) compared to A. tropicalis from both HD_MP (0.18 ± 0.19‰2) and LD_RhB (0.21 ± 0.15‰2). However, the isotopic niche width of A. gazella reduced in size by more than half (0.40 ± 0.40‰2) during winter when the mature females were no longer constrained by the lactation mandate. The mean δ15N values in whiskers of both A. tropicalis and A. gazella in summer were similar (11.3 ± 0.4‰ and 11.0 ± 0.9‰, respectively). The A. gazella broadens its’ isotopic niche feeding at different trophic levels in summer (lactation period). We used a Repeatability index (R) to assess levels of intra- and inter-individual consistency. Repeatability indicated that the bigger isotopic niche width for A. gazella is attributed to high inter- and intra-individual variability in their resource use. The A. gazella individuals ranging closer to the colony consistently consumed myctophid fish. Other individuals foraged on low 15N content euphausiids (i.e., Euphausia spp. and Thysanoessa spp.) south of Marion Island in both winter and summer. The A. tropicalis from LD_RhB were repeatable in their δ15N values (trophic level) (Rglobal = 0.63; [95% CI: 0.29 – 0.80]) and δ13C values (habitat) (Rglobal = 0.69; [95% CI: 0.35 – 0.84]). At HD_MP, the A. tropicalis colony, diet was moderately repeatable (Rglobal = 0.33; [95% CI: 0.11 – 0.51]) while habitat was repeatable (Rglobal = 0.58; [95% CI: 0.33 – 0.73]). Consistency in diet (Rglobal = 0.27; [95% CI: 0.08 – 0.44] and foraging habitat (Rglobal = 0.45; [95% CI: 0.21 – 0.63] was moderate at HD_WT, the A. gazella colony. The diet varied with habitat at the individual level, across seasons. Segregation in diet and foraging habitat minimises resource-use overlap between sympatric fur seals. The behaviour of A. gazella from HD_WT (a high-density colony) might have a direct or indirect impact on the A. tropicalis from LD_RhB (a low-density colony) (i.e., competitive exclusion). Seasonality, at-sea movement, and intrinsic factors shaped the foraging behaviour and diet of adult female fur seals breeding at Marion Island. Some breeding mature females changed diets between seasons and depending on foraging habitat. / Dissertation (MSc (Zoology))--University of Pretoria, 2021. / NRF, Grantholder linked / Zoology and Entomology / MSc (Zoology) / Unrestricted

Stemflow creates biogeochemical hotspots at tree bases. Few studies examine bark structure effects on stemflow generation via stable isotopes. Stemflow volume and isotopic composition (δD, δ18O) were measured over 15 months to address three main objectives: to determine origins and pathways of stemflow, to identify differences in stemflow generation mechanisms between tree species, and to identify differences in stemflow generation mechanisms between meteorological events. Laser ablation spectroscopy showed that, compared to throughfall and precipitation, stemflow was isotopically enriched, signifying isotopic fractionation. A bark-wetting experiment showed bark water storage capacity to be greatest in species with thick, continuous bark. Isotopic composition of precipitation was significantly more enriched in convective storms compared to that of continental or oceanic origin. Therefore, isotopic fractionation of stemflow and stemflow generation mechanisms vary from that of throughfall and precipitation, by interspecific differences in bark, and by meteorological event, potentially influencing existing canopy water storage models.