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A comparative study of how high school students understand stem cells /Moyer, Jonathan Christian Rabe, January 2007 (has links) (PDF)
Thesis (M.S.) in Teaching--University of Maine, 2007. / Includes vita. Includes bibliographical references (leaves 63-64).
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Natural and modified variations in human induced pluripotent stem cellsRouhani, Foad Jafari January 2015 (has links)
No description available.
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FACTORS INFLUENCING THE RETENTION OF A SAMPLE OF WOMEN IN STEM IN THE UNITED STATESNorris, Christine Frances 01 August 2019 (has links)
Since 1990, the percentage of women in STEM in the United States has not increased (Landivar, 2013). Women make up only 24% of the STEM workforce whereas they make up around 50% of the workforce across all occupations (Beede et al., 2011). Thus, there is a deficit of women in STEM. Much of the research available addressing this concern is focused on what interests women in STEM. Very little research is available with women truly working in STEM fields or addressing the retention of women in STEM. In the current study, I investigated factors that may play a role in the retention of women in STEM. I hypothesized that there would be differences in communal goal endorsement, career adaptability, stereotype threat, sexual harassment, and work satisfaction between women who remain in STEM and those who departed from STEM. Data was collected via self-report survey from 212 women currently or previously in STEM. Multivariate analysis was used to examine the hypotheses. Overall, the MANCOVA was not significant, indicating that women who remained in STEM were not different than women who departed from STEM in terms of the hypothesized variables of communal goals, career adaptability, stereotype threat, sexual harassment, or job satisfaction. However, rich information regarding women’s experiences of sexual harassment in STEM was gained. Practical and research implications are discussed.
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Focal adhesion kinase regulation of human embryonic stem cellsVitillo, Loriana January 2014 (has links)
Undifferentiated human embryonic stem cells (hESCs) grow on the extracellular matrix (ECM) substrate fibronectin (FN) in defined feeder-free conditions. The ECM is part of the hESCs pluripotent niche and supports their maintenance, but the contribution to survival remains to be elucidated. Understanding the mechanism of survival is particularly crucial in hESCs, since it affects their expansion in cell culture and ultimately translation of research to the clinic. HESCs bind to FN mainly via alpha5β1- integrin, known to be upstream of important survival cascades in other cell types. However, it is not understood if and how FN/integrin binding supports those molecular pathways in the context of pluripotent hESCs. The aim of this work was to elucidate the survival cascade downstream of the FN/integrin interaction in hESCs. Initially, when hESCs were cultured on a non-integrin activating substrate they initiated an apoptotic response that also occurred when β1-integrin was selectively blocked with antibody, leading the cells to detach from FN. Integrin activation is generally transduced within cells via a complex adhesome of scaffold and kinase proteins, among which the focal adhesion kinase (FAK) plays a key role. Indeed, blocking β1-integrin resulted in dephosphorylation of endogenous FAK in hESCs. When FAK kinase activity was directly inhibited (with small molecule inhibitors), hESCs responded by detaching from FN and activating caspase-3, leading to an increase in apoptosis. Furthermore, flow cytometry analysis showed that the population of hESCs that underwent apoptosis still retained the pluripotency-associated marker NANOG. FAK is a convergent point between growth factor signaling and the PI3K/Akt pathway, with a well-reported role in the maintenance of hESCs. Consistently, FN activated both AKT and its target the ubiquitin ligase MDM2 at the protein levels, while pAkt was reduced after β1-integrin blocking and FAK inhibition. Cell imaging showed that MDM2, which regulates p53 degradation in the nucleus, displayed reduced nuclear localisation after FAK inhibition, opening the possibility for a change in the p53 balance in hESCs. In fact, p53 protein increases after FAK inhibition corresponding also to caspase activation. Further investigation explored if FAK-dependent pathways are also implicated in the maintenance of hESC pluripotency. Inhibition of FAK led the cells that survived apoptosis to lose stem cell morphology, decrease pluripotency-associated markers and change nuclear shape. Moreover, a small pool of FAK was found in the nucleus of hESCs cultured on FN, but decreased after FAK inhibition. FAK was also co- immunoprecipitated with NANOG protein in standard hESC culture while NANOG decreased after sustained FAK inhibition. This data suggests that nuclear roles of FAK could support, together with the cytoplasmic activation of the PI3K cascade, both survival and pluripotency pathways requiring further investigation. In conclusion, the original contribution of this work is to identify in FAK the downstream survival effector of the FN/β1-integrin interaction in hESCs. HESCs survival is maintained by the binding of β1-integrin to FN and activation of FAK kinase and downstream PI3K/Akt, leading to the suppression of p53 and caspase activation. In parallel, promotion of these pathways by FAK is suggested also to support the key pluripotency circuitry, feeding into NANOG. Overall, FAK is proposed here as an important regulator of hESC survival and fate.
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Genome-wide transcriptional characterisation and investigation of the murine niche for developing haematopoietic stem cellsMcGarvey, Alison Clare January 2017 (has links)
Haematopoietic stem cells (HSCs) are capable of differentiation into all mature haematopoietic lineages, as well as long-term self-renewal and are consequently able to sustain the adult haematopoietic system throughout life. Currently, in the mouse, HSCs are understood to first appear in the aorta-gonad-mesonephros (AGM) region at embryonic day 11 via a process of maturation from precursors (pre-HSCs). This maturation within the AGM region involves the complex interplay of signalling between cells of the niche and maturing precursor cell populations, but is relatively little understood at a molecular level. Recently our understanding of the AGM region has been refined, identifying the progression from E9.5 to E10.5 and the polarity along the dorso-ventral axis as clear demarcations of the supportive environment for HSC maturation. In this thesis, I investigated the molecular characteristics of these spatio-temporal transitions in the AGM region through the application of RNA-sequencing. This enabled the identification of molecular signatures which may underlie the supportive functionality of the niche. I further compared these expression signatures to the transcriptional profile of an independent cell type, also capable of supporting HSC maturation, the OP9 stromal cell line. By combining this transcriptional information with an ex vivo culture system, I screened a number of molecules for their ability to support HSC maturation from early precursors, leading to the discovery of a novel regulator of HSC maturation: BMPER. Further characterisation of this molecule enabled the identification of its specific cellular source and the proposal that through its action as an inhibitor of BMP signalling it facilitates the maturation of precursors into HSCs. These results lend further detail and support to the role of BMP signalling in the regulation of HSC maturation as well as demonstrating the potential of these transcriptional profiles to yield novel mechanistic insight.
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The developmental potential of adult mouse hair bulge stem cells. / 成體小鼠毛囊隆突幹細胞的發育潛能研究 / Cheng ti xiao shu mao nang long tu gan xi bao de fa yu qian neng yan jiuJanuary 2008 (has links)
Wong, Wai Chung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2008. / Includes bibliographical references (leaves 113-130). / Abstracts in English and Chinese. / Thesis/Assessment Committee --- p.i / Abstract --- p.ii / 中文摘要 --- p.iv / Acknowledgements --- p.vi / List of Figures --- p.vii / List of Tables --- p.ix / Table of Abbreviations --- p.x / Contents --- p.xv / Chapter Chapter I --- Introduction / Chapter 1.1 --- Stem cells --- p.1 / Chapter 1.1.1 --- Embryonic stem cells --- p.2 / Chapter 1.1.2 --- Adult stem cells --- p.2 / Chapter 1.1.3 --- Artificial stem cells --- p.5 / Chapter 1.2 --- Hair bulge stem cells (HBSC) --- p.7 / Chapter 1.2.1 --- Hair follicle --- p.7 / Chapter 1.2.2 --- The discovery of hair bulge stem cells --- p.9 / Chapter 1.2.3 --- The hair bulge niche --- p.10 / Chapter 1.2.4 --- Molecular markers --- p.12 / Chapter 1.2.5 --- Physiological roles of bulge stem cells --- p.13 / Chapter 1.2.6 --- Pathological roles of bulge stem cells --- p.15 / Chapter 1.2.7 --- Developmental plasticity of bulge stem cells --- p.16 / Chapter 1.3 --- "Regulation of adipogenic, osteogenic and cardiogenic differentiation" --- p.17 / Chapter 1.3.1 --- Regulation of adipogenic differentiation --- p.17 / Chapter 1.3.2 --- Regulation of osteogenic differentiation --- p.18 / Chapter 1.3.3 --- Regulation of cardiogenic differentiation --- p.19 / Chapter 1.4 --- Proteomics --- p.23 / Chapter 1.4.1 --- Definition of proteomics --- p.23 / Chapter 1.4.2 --- Two-dimensional gel electrophoresis (2DGE) --- p.25 / Chapter 1.4.3 --- Mass spectrometry and protein identification --- p.29 / Chapter 1.4.4 --- Other techniques associated with proteomics --- p.34 / Chapter 1.4.5 --- Proteomics and stem cells --- p.36 / Chapter 1.5 --- General summary --- p.37 / Chapter 1.6 --- Aims of my study --- p.38 / Chapter Chapter II --- Materials and Methods / Chapter 2.1 --- Animals --- p.39 / Chapter 2.2 --- Immunohistochemistry --- p.39 / Chapter 2.2.1 --- Histology --- p.39 / Chapter 2.2.2 --- Immunohistochemistry --- p.40 / Chapter 2.3 --- Establishment of hair bulge CD34+ stem cell line --- p.41 / Chapter 2.3.1 --- Isolation of hair bulge explants --- p.41 / Chapter 2.3.2 --- Establishing hair bulge primary cultured cells --- p.42 / Chapter 2.3.3 --- Purification of hair bulge stem cells --- p.43 / Chapter 2.4 --- Karyotyping --- p.45 / Chapter 2.5 --- In vitro differentiation --- p.46 / Chapter 2.5.1 --- Adipogenic differentiation --- p.46 / Chapter 2.5.2 --- Osteogenic differentiation --- p.47 / Chapter 2.5.3 --- Cardiogenic differentiation --- p.48 / Chapter 2.6 --- Proteomic analysis --- p.48 / Chapter 2.6.1 --- Sample preparation --- p.48 / Chapter 2.6.2 --- Quantification of proteins --- p.49 / Chapter 2.6.3 --- First-dimensional separation of proteins 226}0ؤ isoelectric focusing (IEF) --- p.50 / Chapter 2.6.4 --- Second-dimensional separation - sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.51 / Chapter 2.6.5 --- "Silver staining, imaging and destaining" --- p.52 / Chapter 2.6.6 --- In-gel digestion and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analysis --- p.53 / Chapter 2.7 --- Histochemistry --- p.54 / Chapter 2.7.1 --- Oil Red O staining --- p.54 / Chapter 2.7.2 --- Alizarin Red S staining --- p.55 / Chapter 2.8 --- Immunocytochemistry --- p.56 / Chapter 2.9 --- Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) --- p.57 / Chapter 2.9.1 --- Isolation of total cellular RNA --- p.57 / Chapter 2.9.2 --- Complementary DNA (cDNA) synthesis --- p.58 / Chapter 2.9.3 --- Polymerase chain reaction and agarose gel electrophoresis --- p.59 / Chapter 2.10 --- Western blot analysis --- p.62 / Chapter 2.10.1 --- Sample preparation and quantification of proteins --- p.62 / Chapter 2.10.2 --- SDS-PAGE --- p.63 / Chapter 2.10.3 --- Protein transfer --- p.64 / Chapter 2.10.4 --- Immunodetection --- p.65 / Chapter 2.11 --- Cell proliferation assay --- p.66 / Chapter 2.11.1 --- Determination of growth pattern --- p.66 / Chapter 2.11.2 --- MTT assay --- p.66 / Chapter 2.12 --- Ultrastructural analysis --- p.67 / Chapter 2.12.1 --- Scanning electron microscopy (SEM) --- p.67 / Chapter 2.12.2 --- Transmission electron microscopy (TEM) --- p.68 / Chapter 2.13 --- Statistical analysis --- p.68 / Chapter Chapter III --- Results / Chapter 3.1 --- Isolation and characterization hair bulge stem cells --- p.69 / Chapter 3.2 --- Directed adipogenic differentiation --- p.70 / Chapter 3.3 --- Directed osteogenic differentiation --- p.71 / Chapter 3.4 --- Ability of cardiogenol C to induce cardiogenesis in HBSCs --- p.71 / Chapter 3.5 --- Comparative proteomic analysis of HBSC cardiogenic differentiation induced by cardiogenol C --- p.72 / Chapter 3.6 --- Role of Wnt signaling pathway in cardiogenol C-induced cardiogenesis --- p.74 / Chapter 3.7 --- Role of chromatin remodeling in cardiogenol C-induced cardiogenesis --- p.75 / Chapter 3.8 --- Legends and tables --- p.76 / Chapter Chapter IV --- Discussion --- p.98 / References --- p.113 / Appendices --- p.131 / Publication --- p.134
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Characterization and therapeutic transplantation of stem cells /Meyer, Jason S., January 2004 (has links)
Thesis (Ph.D.)--University of Missouri-Columbia, 2004. / Typescript. Vita. Includes bibliographical references (leaves 153-173). Also available on the Internet.
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Characterization and therapeutic transplantation of stem cellsMeyer, Jason S., January 2004 (has links)
Thesis (Ph.D.)--University of Missouri-Columbia, 2004. / Typescript. Vita. Includes bibliographical references (leaves 153-173). Also available on the Internet.
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Diverse Learners in the Classroom: Students with Special Needs Enrolled in Science, Technology, Engineering, and Mathematics (STEM) Texas Public ClassroomsBriones, San Juanita G 08 1900 (has links)
The purpose of this study was to determine if students with special needs participating in an inclusive classroom can learn the skills related to a STEM career as compared to the general student population. The study involved seventh grade students from two rural middle schools in north central Texas and was framed through a constructivist lens using a quasi-experimental design with a convenience sample. The Solenoid Invention Kit Assessment and the STEM Semantics Survey used in this study were used from a previously large existing dataset from a grant funded by the National Science Foundation for Innovative Technology Experiences for Students and Teachers. Findings suggested that there were no significant differences between the general student population and students with special needs. However, STEM coursework in an inclusive classroom may impact students' decision to pursue STEM careers.
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The Apparent Heterogeneity of the National Stem Landscape: Does it Reflect Reality or is it an Illusion?Miller, Kurtz K. January 2013 (has links)
No description available.
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