Thesis (M.S.)--University of Michigan, 1980.

Thesis (M.S.)--University of Michigan, 1980.

Thesis (Ph.D.)--Mississippi State University. Department of Agricultural Information Science and Education. / Title from title screen. Includes bibliographical references. Available also in print.

The present study considers the versatile role of adipokinetic hormones (AKHs). Using the firebug Pyrrhocoris apterus as a model insect, the hypothesis that AKHs mediate stress response mechanisms was explored. The outcome indicated that there is a positive feedback regulation between an oxidative stressor action and the level of AKH in insect body, and that AKHs might be involved in the activation of antioxidant protection mechanism. Further results revealed a functional homology between AKH and the mammalian hormone glucagon. The possible effects of glucagon on mobilisation of energy reserves and on elicitation of an antioxidant response to oxidative stress were investigated. As a result, glucagon-immunoreactive material was detected for the first time in the firebug central nervous system and gut. Antioxidant mechanisms are elicited after glucagon treatment but it did not involve mobilization of energy reserves or AKH level changes. As a complement, the existence of a feedback between juvenile hormone and AKH was investigated by topical application of the juvenile hormone analogue methoprene, which influenced the release of AKH from the central nervous system into the haemolymph and induced a partial reduction of lipid content in haemolymph.

This diploma thesis deals with influence of a notch on stress-strain states at the front of cracks by shear modes. Starting with fracture mechanics and its division, followed by stress intensity factor and calculate its by finite element method. Calculation is solved for two types of notches, U-notched and V-notched, both notches were modeled parametrically so their geometry was changeable and stress intensity factor were calculated for all configurations. Subsequently was solved next calculation of stress intensity factor but for shaft without notch. Finally, was evaluated influence of notch on stress intensity factor. Software for finite element method has been used ANSYS. Others calculation was provided in software MATLAB

Master's thesis deals with the stress applied to the employee in a work process in the company ABC, Ltd. It focuses on the factors that cause stress. It examines its manifestations and effects on health and work performance of employees. The theoretical part includes an overview of this issue. The practical part is analytical, processing questionnaires students - future employees and current employees. Visions of students at secondary and university level of stress that occurs in the process, are compared to the fact how stress manifests itself directly to employees in working conditions. The conclusion presents proposals to reduce or eliminate stress and increase resilience against him.

Several functions have been attributed to the cellular prion protein, PrPc, amongst which its anti-oxidant role has rapidly been gaining interest in the recent years. We and others have previously shown, that PrPc expressing cells, of neuroblastoma or epithelial origin, seem to exhibit a higher overall viability towards paraquat toxicity than cells expressing basal or low levels of the protein. Although several studies propose a protective mechanism that involves PrPc dependent activation of the superoxide dismutase (SOD) enzymatic machinery or an activation of its own intrinsic antioxidant function, others argue against this SOD-like role. Our objective was to investigate, at a biological and proteomic level, by which potential mechanism PrPc could protect neuroblastoma cells against paraquat induced oxidative damage. Using a biological aproach, we firstly evaluated the status of the Cu/Zn-SOD enzyme in Human neuroblastoma cells expressing different forms of PrPc following their exposure to paraquat. Next, we performed a proteomic study to investigate by which other potential mechanism(s), PrPc could protect the cell against paraquat induced oxidative stress. Our proteomic approach made use of an optimised two-dimensional liquid chromatography system, the ProteomeLab PF-2D, and reverse phase chromatography coupled with lava purple stained SDS-PAGE, both interfaced with tandem mass spectrometry. An interesting aspect of our study has been the development of an original immunoproteomic technique called immuno-PF2D-MS/MS, coupling classical immunological methods to a two-dimensional liquid chromatography proteomic tool interfaced with tandem mass spectrometry. We have proposed this technique for antigenic and serological characterization that have important implications in the study of biomarkers. Another important aspect of our study has been the detection of several candidates that could participate in PrPc-mediated protection against paraquat induced oxidative stress. Although, it was out of our scope to investigate each of these candidates in the present study, it presents an interesting perspective for future studies. We have, however, shown the implication of one such candidate: PARP-1. Complimentary tests will be necessary in the future to confirm the actual interaction of this candidate with PrPc.

Une des hypothèses concernant la fonction du sommeil suggère que ce dernier permettrait la plasticité neuronale et l'organisation (ou la réorganisation) synaptique, phénomènes sous-tendant des fonctions cognitives. Des perturbations spécifiques du sommeil faisant suite à un apprentissage ont en effet montré une diminution significative des performances aux niveaux des gènes et du comportement. Notre travail à visé à étudier les conséquences d'une privation de sommeil faisant suite à un apprentissage d'une tâche mnésique au niveau de l'abondance de protéines dans l'hippocampe de rat. Pour ce faire, une première étude protéomique de l'hippocampe de rat en l'absence d'apprentissage spatial spécifique a d'abord été réalisée ; elle montre l'absence de différence quantitative d'abondance protéique entre les hippocampes gauche et droit. Ensuite, une seconde étude protéomique montre qu'une privation de courte durée affecte différents réseaux de protéines, principalement liés au métabolisme cellulaire, aux voies biochimiques de l'énergie, des transports, du trafic vésiculaire, du cytosquelette et du traitement des protéines dans l'hippocampe de rat. Finalement, une troisième étude protéomique montre les effets d'un apprentissage d'une tâche spatiale en début de période d'activité diurne sur le protéome d'hippocampe de rat. Les principales protéines affectées ont ici des fonctions liées au métabolisme cellulaire et au cytosquelette.

The objective of this study is to model and analyze a three dimensional single riveted lap joint (with and without a crack). By using finite element method, stress and fracture analyses are carried out under both the residual stress field and external tensile loading. Using a two step simulation, riveting process and subsequent tensile loading of the lap joint are simulated to determine the residual and overall stress state. Residual stress state due to riveting is obtained by interference and clamping misfit method. By employing different interference and clamping misfit values, the effects of riveting process parameters on stress state are examined. Two cracks namely the semi elliptical surface crack at faying surfaces of plates and the quarter elliptical corner crack at rivet hole are the most widely observed crack types in riveted joints. Fracture analysis of cracked riveted joints is carried out by introducing these two crack types to the outer plate at a plane perpendicular to the loading. The mixed mode stress intensity factors (SIFs) and energy release rates (G) around the crack front are obtained by using displacement correlation technique (DCT). Effects riveting process parameters (interference and clamping ratios) and geometrical parameters (crack shape and size) on fracture parameters are studied. The stress intensity factor solutions presented herein could be useful for correlating fatigue crack growth rates, fracture toughness computation, and multiple site damage (MSD) analysis in aircraft bodies.

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2002. / Härtill 5 uppsatser.