Constrained sintering is an important process for many applications. The sintering process almost always involves some form of constraint, both internal and external, such as rigid particles, reinforcing fibers and substrates to which the porous body adheres. The densification behavior of zinc oxide and cordierite-base crystallizable glass constrained on a rigid substrate was studied to add to the understanding of the behavior of various materials undergoing sintering when subjected to external substrate constraint. Porous ZnO films were isothermally sintered at temperatures between 900°C and 1050°C. The results showed that the densification of films constrained on substrates is severely reduced. This was evident in the sintered microstructures where the particles are joined together by narrower necks forming a more open structure, instead of the equiaxed grains with wide grain boundaries observed in the freestanding films. The calculated activation energies of densification were also different. For the density range of 60 to 64%, the constrained film had an activation energy of 391 ± 34 kJ/mole compared to 242 ± 21 kJ/mole for the freestanding film, indicating a change in the densification mechanism. In-plane stresses were observed during the sintering of the constrained films. Yielding of the films, in which the stresses dropped slight or remained unchanged, occurred at relative densities below 60% before the stresses climbed linearly with increasing density followed by a gradual relaxation. A substantial amount of the stresses remained after cooling. Free and constrained films of the cordierite-base crystallizable glass (glass-ceramic) were sintered between 900°C and 1000°C. The substrate constraint did not have a significant effect on the densification rate but the constrained films eventually underwent expansion. Calculations of the densification activation energy showed that, on average, it was close to 1077 kJ/mole, the activation energy of the glass, indicating that the prevailing mechanism was still viscous flow. The films expanded earlier and faster with increasing sintering temperature. The expansion was traced to the formation of pores at the interface with the silicon substrate and to a lesser extent on aluminum nitride. It was significantly reduced when the silicon substrate was pre-oxidized at 900°C, leading to the conclusion that the pore formation at the interface was due to poor wetting, which in turn was caused by the loss of the thin oxide layer through a reaction with the glass. / Ph. D.

Plasmodium falciparum dipeptidyl aminopeptidase 1 (DPAP1) contributes to the degradation of hemoglobin by releasing dipeptides from globin oligopeptides in the food vacuole. The lack of success at DPAP1 gene disruption suggests that this exopeptidase is important for efficient growth during the erythrocytic asexual stage. DPAP1 is therefore an attractive target for the development of anti-malarial drugs that block the catabolism of hemoglobin. To guide the design of selective, potent DPAP1 inhibitors, it is necessary to characterize the substrate specificity of this enzyme along with its human homolog cathepsin C. Although native purification of DPAP1 is possible, the amount of purified enzyme obtained is insufficient for extensive biochemical characterization. To overcome this obstacle, a strategy was developed for the recombinant expression of soluble DPAP1 in the bacterium Escherichia coli and for its activation in vitro. The production of active recombinant DPAP1 presents three challenges: 1) expression of the protein in soluble form, 2) generation of the native N-terminus, and 3) cleavage of the pro-domain. Soluble expression of DPAP1 was achieved by fusing it to the C-terminus of maltose-binding protein (MBP). A linker sequence encoding a tobacco etch virus protease (TEVp) cleavage site was introduced between MBP and DPAP1 such that TEVp cleavage would generate the presumed native N-terminus of DPAP1. Incubation of the MBP-DPAP1 fusion with TEVp resulted in the release of free DPAP1which hydrolyzed the fluorogenic substrate proyly-arginyl-7-amido-4 methyl coumarin (Pro-Arg-AMC). Various proteases were tested for the ability to excise the pro-region. Treatment with both trypsin and papain removed the pro-region and increased DPAP1 activity two to three fold. When assayed with Pro-Arg-AMC, trypsin-treated DPAP1 had kinetic properties similar to native enzyme whereas papain-treated DPAP1 deviated from Michaelis-Menten kinetics. Using a combinational dipeptidyl substrate library, the substrate specificities of native and recombinant (trypsin-activated) DPAP1, as well as of human cathepsin C were profiled. We find that both DPAP1 and human cathepsin C accept a wide spectrum of amino acid side chains at the substrate P1 and P2 positions. Interestingly, several P2 residues show high selectivity for DPAP1 or cathepsin C. The collected data point to the feasibility of designing inhibitors that are specific for DPAP1 over cathepsin C. / Master of Science in Life Sciences

Point-of-load (POL) converters have been used extensively in IT products. Today, almost every microprocessor is powered by a multi-phase POL converter with high output current, which is also known as voltage regulator (VR). In the state-of-the-art VRs, the circuits are mostly constructed with discrete components and situated on the motherboard, where it can occupy more than 1/3 of the footprint of the motherboard. A compact POL is desirable to save precious space on motherboards to be used for some other critical functionalities. Recently, industry has released many modularized POL converters, in which the bulky inductor is integrated with the active components to increase the power density. This concept has been demonstrated at current levels less than 5A and power density around 600-1000W/in³. This might address the needs of small hand-held equipment such as smart phones, but it is far from meeting the needs for the applications such as laptops, desktops and servers, where tens and hundreds of amperes are needed. A 3D integrated POL module with an output current of tens of ampere has been successfully demonstrated at the Center for Power Electronic Systems (CPES), Virginia Tech. In this structure, the inductor is elaborated with low temperature co-fire ceramic (LTCC) ferrite, as a substrate where the active components are placed. The lateral flux inductor is proposed to achieve both a low profile and high power density. Generally, the size of the inductor can be continuously shrunk by raising the switching frequency. The emerging gallium-nitride (GaN) power devices enable the creation and use of a multi-MHz, high efficiency POL converter. This dissertation firstly explores the LTCC inductor substrate design in the multi-MHz range for a high-current POL module with GaN devices. The impacts of different frequencies and different LTCC ferrite materials on the inductor are also discussed. Thanks to the DC flux cancellation effect, the inverse coupled inductor further improves the power density of a 20A, 5MHz two-phase POL module to more than 1kW/in³. An FEA simulation model is developed to study the core loss of the lateral flux coupled inductor, which shows the inverse coupling is also beneficial for core loss reduction. The ceramic-based 3D integrated POL module, however, is not widely adopted in industrial products because of the relatively high cost of the LTCC ferrite material and complicated manufacturing process. To solve that problem, a printed circuit board (PCB) inductor substrate with embedded alloy flake composite core is proposed. The layerwise magnetic core is laminated into a multi-layer PCB, and the winding of the inductor then is formed by the copper layers and conventional PCB vias. As a demonstration of system integration, a 20A, 1.5MHz integrated POL module is designed and fabricated based on a 4-layer PCB with embedded flake core, which realizes more than 85% efficiency and 600W/in³ power density. The application of standardized PCB processes reduces the cost for manufacturing the integrated modules due to the easy automation and the low temperature manufacturing process. Combining the PCB-embedded coupled inductor substrate and advanced control strategy, the two-phase 40A POL modules are elaborated as a complete integrated laptop VR solution. The coupled inductor structure is slightly modified to improve its transient performance. The nonlinearity of the inductance is controlled by adding either air slots or low permeability magnetic slots into the leakage flux path of the coupled inductor. Then the leakage flux, which determines the transient response of the coupled inductor, can be well controlled. If we directly replace the discrete VR solution with the proposed integrated modules, more than 50% of the footprint on the motherboard can be saved. Although the benefits of the lateral flux inductor have been validated in terms of its high power density and low profile, the planar core is excited under very non-uniform flux. Some parts of the core are even pushed into the saturation region, which totally goes against the conventional sense of magnetic design. The final part of this dissertation focuses on evaluating the performance of the planar core with variable flux. The counterbalance between DC flux and AC flux is revealed, with which the AC flux and the core loss density are automatically limited in the saturated core. The saturation is essentially no longer detrimental in this special structure. Compared with the conventional uniform flux design, the variable flux structure extends the operating point into the saturation region, which gives better utilization of the core. In addition, the planar core with variable flux also provides better thermal management and more core loss reduction under light load. As conclusions, this research first challenges the conventional magnetic design rules, which always assumes uniform flux. The unique characteristics and benefits of the variable flux core are proved. As an example of taking advantages of the lateral flux inductor, the PCB integrated POL modules are proposed and demonstrated as a high-density VR solution. The integrated modules are cost-effective and ready to be commercialized, which could enable the next technological innovation for the whole computing and telecom industry. / Ph. D.

Soilless substrates, such as peat, pine bark, and coir, are widely used as growing media in containerized crops for their favorable characteristics, including low bulk density, balanced air exchange and water retention, disease resistance, and low pH and salinity. However, improper irrigation of these media can have negative outcomes such as root asphyxia, pathogen development, and reduced plant growth. Understanding pore size distributions, water dynamics, and gas diffusivity of these substrates is essential to promote plant growth. The effects of different particle sizes of soilless media on processes such as infiltration, hydraulic conductivity, and gas diffusivity are also not well understood. The characterization of these effects is important for the overall improvement of container crop production. This thesis presents three studies that aimed to characterize the physical and hydraulic properties of pine bark substrates, both unamended and amended with peat or coir. The first study looked at three substrate types: unamended, unscreened pine bark, peat-amended pine bark, and coir amended pine bark. Three methods were employed to quantify pore distributions: non-equilibrium infiltration measurements, equilibrium water retention characterization, and scanning electron microscopy. We characterized pore distributions during wetting and drainage for the three substrates. Coir-amended bark had the largest water-conducting porosity, highest hydraulic conductivity, and most water retention. Unamended pine bark had the highest microporosity, and the addition of peat and coir lowered macroporosity, with peat having the greater effect. The total porosity inferred from the infiltration method was significantly smaller than that inferred from drainage experiments due to assumptions related to pore shape. The second study focused on defining hydraulic conductivity and water retention for pine bark substrates of five different particle sizes, <1 mm, 1-2 mm, 2-4 mm, 4-6 mm, and an unscreened fraction. We utilized the same methods from the first study. The resulting data showed that the smallest particle sizes (i.e., <1 mm and 1-2 mm) had the highest hydraulic conductivity and greatest water retention. The three larger sizes had lower hydraulic conductivity and poor water retention, including the unscreened fraction, which more closely followed the results of the 2-4 mm size. The final study examined gas diffusivity of the five pine bark particle sizes at different moisture levels: 60% moisture content (initial conditions), saturated at the bottom of the sample, near-saturated at the sample bottom, and drained from saturation to container capacity. We used a one-chamber gas diffusion setup to find gas diffusion coefficients (Ds). The results displayed an inverse relationship between Ds values and substrate water content. In addition, the larger particle sizes were less sensitive to changes in water content due to their well-draining large pores. Proper balance of aeration and water retention is necessary for the success of soilless growing media. Overall, the smaller particle size fractions had the best water retention and hydraulic conductivity rates while the larger fractions had the largest Ds coefficients. This work contributes valuable knowledge on the physical and hydraulic properties of different size fractions of pine bark substrates, which can assist nursery growers in optimizing water usage for sustainable container crop production. / Master of Science / Since the 1950's soilless substrates have been an important resource for growing a variety of fruits, vegetables, flowers, and ornamental plants. Soilless growing media have become more popular choices for containerized plant production compared to natural soils due to improved air exchange, increased disease resistance, and more plants per acre. They are also favored because they help conserve resources, reduce agricultural waste, and minimize transportation requirements as compared to traditional cropping methods. The most popular types of soilless media include peat, coir, compost, and pine bark. In the U.S., pine bark is the main substrate used, as it is renewable and widely available. Growers still face many issues when using containerized crop production. For example, pine bark is susceptible to water runoff which can cause environmental problems and increase costs from this loss of water and fertilizer. Further characterizing of water and gas dynamics in of pine bark growing media is important for conserving water and fertilizer resources while optimizing plant growth in this container cropping industry. Pore characteristics, aeration, and water movement are key factors of substrates to be described to solve these challenges. This project aimed to apply soil physics strategies to soilless media, focusing on describing pore sizes, water movement, water holding capacity, and air movement in pine bark substrates. We utilized three methods throughout this study. For the first method, we took infiltration measurements to examine how water moved into the media, while the second utilized controlled drainage experiments to observe how water moved out of the media. The final method was characterizing gas movement through the substrates at different water contents and particle sizes. The results found showed that the smaller particle sizes and pine bark mixed with peat and coir had increased ability to retain water and allow water movement as compared to the larger particle sizes and unamended pine bark. In contrast, the larger particles had less water retention but improved gas movement. These results could be applied by stacking different particle sizes or mixes over one another could optimize water retention in the top of the container and drainage and gas movement in the bottom of the container. Overall, the application of this work is to create best management practices for growers to be able to balance water retention and gas movement in order to optimize plant growth.

Yes / This study aims to introduce a new phased array design with improved radiation properties for future cellular networks. The procedure of the array design is simple and has been accomplished on a low-cost substrate material while offering several interesting features with high performance. Its schematic involves eight air-filled slot-loop metal-ring elements with a 1 × 8 linear arrangement at the top edge of the 5G smartphone mainboard. Considering the entire board area, the proposed antenna elements occupy an extremely small area. The antenna elements cover the range of 21–23.5 GHz sub-mm-wave 5G bands. Due to the air-filled function in the configurations of the elements, low-loss and high-performance radiation properties are observed. In addition, the fundamental characteristics of the introduced array are insensitive to various types of substrates. Moreover, its radiation properties have been compared with conventional arrays and better results have been observed. The proposed array appears with a simple design, a low complexity profile, and its attractive broad impedance bandwidth, end-fire radiation mode, wide beam steering, high radiation coverage, and stable characteristics meet the needs of 5G applications in future cellular communications. Additionally, the smartphone array design offers sufficient efficiency when it comes to the appearance and integration of the user’s components. Thus, it could be used in 5G hand-portable devices.

Phase shifters play a crucial role in radar, satellite communications, and 5G networks. Recently, the idea of using filters as phase shifters have attracted much interest due to providing many benefits such as smaller area, lower noise figure, easier fabrication method as compared with other technologies, reduced cost, and the ability to work as multi-functioning device. This work focuses on the implementation of bandstop-based and bandpass-based filtering phase shifters. For the bandstop-based approach, some of the resonating structures that were examined are stubs, LC tanks, L-shaped/U-shaped half wavelength resonators and many others. Periodic stub loading filter design method was compared against traditional filter synthesis method in order to determine which approach can provide better insertion loss range, higher phase range, and wider bandwidth. Optimization of stub impedance and the size of the device was presented. With regards to bandpass-based filtering phase shifters, microstrip-based and substrate integrated waveguide-based designs were investigated. The benefits of microstrip technology include low profile, low cost, ease of fabrication and integration. Microstrip-based designs were implemented using square shaped and octagon shaped split ring resonators. Then, substrate integrated waveguide (SIW) cavity-based designs were proposed and realized using hexagon resonators. The advantages of SIW-based filters are low insertion loss, high power handling, high immunity to external noise and crosstalk. A novel SIW-based filtering phase shifter with tunable transmission zero and tunable center frequency was developed aimed at maximizing device performance.

I released chloride and corn pollen into artificial streams to study the effects of discharge, substrate size, leaf packs, and pools on the retention and transport of fine particulate organic matter (FPOM). I found that doubling discharge significantly decreased both hydraulic and FPOM retention (measured as pollen uptake length). However, quadrupling discharge returned both hydraulic and FPOM retention to original or higher values. There was a strong positive relationship between FPOM depositional velocity and discharge (r² = 0.846, p < 0.0001), and between FPOM depositional velocity and turbulence (r² = 0.831, p < 0.0001). Depositional velocity for all experiments was considerably less than predicted by the “fall-velocity model”. Substrate size controlled hydraulic retention through the size of interstitial spaces in the bed and FPOM retention through substrate-created turbulence. Small gravel substrate had the largest transient storage zone relative to stream cross-sectional area. Large gravel substrate had the highest depositional velocity of pollen and the most turbulence. Cobble had the least hydraulic and FPOM retention. Adding leaf packs significantly decreased hydraulic and FPOM retention. However, the number of leaf packs made little difference. Adding pools significantly increased hydraulic retention and FROM depositional velocity. / Master of Science

Macronutrient metabolism plays an essential role in the overall health of an individual. Depending on a number of variables, for example, diet, fitness level, or metabolic disease state, protein, carbohydrate and fat have varying capacities to be oxidized and balanced. Further, when analyzing the oxidation of carbohydrate and fat in the skeletal muscle specifically, carbohydrate balance happens quite rapidly, while fat balance does not. The ability of skeletal muscle to adapt and respond to various nutrient states is critical to maintaining healthy metabolic function. Habitual high fat intake has been associated with reduced oxidative capacity, insulin resistance, increased gut permeability, inflammation, and other risk factors often preceding metabolic disease states. The disruption of gut function leads to gut permeability and increases endotoxins released into circulation. Endotoxins have been shown to play an important role in obesity-related whole body and tissue specific metabolic perturbations. Each of these disrupted metabolic processes is known to associate with obesity, metabolic syndrome and diabetes. To date, limited research has investigated the role of high fat diet on skeletal muscle substrate oxidation and its relationship to gut permeability and endotoxins. The purpose of this study was to determine the effects of an acute, five-day, isocaloric high fat diet (HFD) on skeletal muscle substrate metabolism in healthy non-obese humans. An additional purpose was to determine the effects of a HFD on gut permeability and blood endotoxins on healthy, non-obese, sedentary humans. Thirteen college age males were fed a control diet for two weeks, followed by five days of an isocaloric HFD. To assess the effects of a HFD on skeletal muscle metabolic adaptability and postprandial endotoxin levels, subjects underwent a high fat meal challenge before and after a HFD. Muscle biopsies were obtained; blood was collected; insulin sensitivity was assessed via intravenous glucose tolerance test; and intestinal permeability was assessed via the four-sugar probe test before and after the HFD. Postprandial glucose oxidation and fatty acid oxidation in skeletal muscle increased before the HFD intervention but was decreased after. Skeletal muscle in vitro assay of metabolic flexibility was significantly blunted following the HFD. Insulin sensitivity and intestinal permeability were not affected by HFD, but fasting endotoxin was significantly higher following the HFD. These findings demonstrate that in young, healthy males, following five days of an isocaloric high fat diet, skeletal muscle metabolic adaptation is robust. Additionally, increased fasting endotoxin independent of gut permeability changes are potentially a contributor to the inflammatory state that disrupts substrate oxidation. These findings suggest that even short-term changes in dietary fat consumption have profound effects on skeletal muscle substrate metabolism and fasting endotoxin levels, independent of positive energy balance and whole-body insulin sensitivity. / Ph. D. / Macronutrients, namely carbohydrates, fats and protein, and the way they are utilized play an important role in the overall health of an individual. Many variables come into play when considering the oxidization (or utilization) of each macronutrient, including, but not limited to diet, fitness level, and metabolic disease state. Skeletal muscle and its role in these processes is of special interest as it is the largest insulin sensitive organ in the body. Its ability to adapt and respond to various nutrient states is critical to maintaining healthy metabolic function. Habitual high fat intake has been associated with insulin resistance, increased gut permeability (increasing endotoxins, which are toxins released into circulation from the intestines), reduced oxidative capacity (ability to utilize macronutrients for energy), and inflammation, all of which are risk factors that precede metabolic disease states. To date, limited research has investigated the role of high fat diet on skeletal muscle oxidation of macronutrients and its relationship to what is going on in the gut, or intestines. The purpose of the study was to determine the effects of a short term high fat diet (five days) on skeletal muscle in healthy, non-obese humans, and to determine the effects of this diet on gut permeability and endotoxins. Thirteen college-age males were fed a control diet for two weeks followed by five days of a high fat diet. Each diet had the same caloric content. Subjects underwent a high fat meal challenge before and after the diet to assess the effects of the diet on skeletal muscle adaptability and post meal endotoxin levels. Before and after the high fat diet, muscle biopsies were obtained, blood was collected, insulin sensitivity was assessed and gut permeability was measured. We found that skeletal muscle metabolic adaptation is robust. Additionally, increased fasting endotoxin changes are a possible contributor to the inflammatory state that disrupts macronutrient oxidation. Therefore, even short-term changes in dietary fat consumption have profound effects on skeletal muscle metabolism and fasting endotoxin levels, independent of positive energy balance and whole-body insulin sensitivity.

Thin films are produced from layers of materials ranging from nanometres to micrometres in height. They are increasingly common and are being used in integrated circuit design, optical coatings, protective coatings, and environmental sensing. Thin films can be fabricated using a variety of methods involving chemical reactions or physical transport of matter. Glancing angle deposition (GLAD) thin films are produced using physical vapour deposition techniques under high vacuum conditions where exploitation of the geometric conditions between the source and the substrate causes enhanced atomic self shadowing to produce structured thin films. This work deals with the modification of these films, emph{in situ} by altering growing conditions through substrate temperatures control, or post-deposition through reactive ion etching (RIE). The first part of the thesis deals with the modification of TiO$_2$ GLAD humidity sensors using RIE with CF$_4$. The data presented demonstrates improved response times to step changes in humidity. Characterization revealed response times of better then 50~ms (instrument-limited measurement). An etch recipe for complete removal of TiO$_2$ was also demonstrated with shadow masking to transfer patterns into GLAD films. The subsequent chapter focuses on modification of thin film growth conditions by increasing adatom mobility. A radiative heating system was designed and implemented with the ability to achieve chuck temperatures of 400$^circ$C. Capping layers on top of GLAD films were grown to demonstrate effects of emph{in situ} heating, and a quantitative analysis of crack reduction with increased temperatures is presented. Lithographic pattern transfer onto a capped GLAD film was demonstrated. Opposite to the goal of the preceding chapter, the focus of the final experimental chapter was to limit adatom mobility. A LN$_2$-based cooling system was designed and implemented for the purpose of studying the growth by GLAD of lower melting point materials, which under regular growth conditions do not form well-defined structures. Chuck temperatures of $-60$$^circ$C can be achieved during deposition while still allowing substrate rotation. The growth of helical copper films was used to demonstrate the effects of emph{in situ} substrate cooling. / Micro-Electro-Mechanical Systems (MEMS) and Nanotechnology

No description available.