SILVA, J. S. P. Estudo das caracter?sticas f?sico-qu?micas e biol?gicas pela ades?o de osteoblastos em superf?cies de tit?nio modificadas pela nitreta??o em plasma. 2008. 119 f. Tese (Doutorado) - Faculdade de Medicina, Universidade de S?o Paulo. S?o Paulo, 2008. / Submitted by CECILIA SANTOS (cecilia@bczm.ufrn.br) on 2010-09-29T13:32:45Z No. of bitstreams: 2 2008Tese_JSandroPS_Estudo.pdf: 2786183 bytes, checksum: a2ca1d8386a1a0d5d0c3befb46b1ed92 (MD5) license_rdf: 20464 bytes, checksum: d540d98f4c56c073d1d99228e27d53ca (MD5) / Approved for entry into archive by clediane guedes(clediane@bczm.ufrn.br) on 2010-09-29T18:15:54Z (GMT) No. of bitstreams: 2 2008Tese_JSandroPS_Estudo.pdf: 2786183 bytes, checksum: a2ca1d8386a1a0d5d0c3befb46b1ed92 (MD5) license_rdf: 20464 bytes, checksum: d540d98f4c56c073d1d99228e27d53ca (MD5) / Made available in DSpace on 2010-09-29T18:15:54Z (GMT). No. of bitstreams: 2 2008Tese_JSandroPS_Estudo.pdf: 2786183 bytes, checksum: a2ca1d8386a1a0d5d0c3befb46b1ed92 (MD5) license_rdf: 20464 bytes, checksum: d540d98f4c56c073d1d99228e27d53ca (MD5) / PURPOSE: The aim of this study was to evaluated the physico-chemical properties of different titanium surfaces modified by means of low temperature plasma nitridind on rat osteoblast cell adhesion and proliferation. METHODS: Pure Titanium discs grade II was submitted to three different surface preparations (polishing, glowglow discharge plasma nitriding in planar and cathodic cage configurations). Surface parameters as roughness, wettability and chemichal composition was determined to compare influency of gas mixture on the modified surface material properties. Cellular morphology was observed by scanning electron microscopy.To evaluate the effect of the surface on cellular response, osteoblast cells (MC3T3) adhesion and proliferation was quantified and data analised by Kruskal-Wallis and Friedman statistical tests. RESULTS: plasma nitriding discs shows rougher surfaces( p<0,02) in cathodic cage configuration andlower contact angle values. MC3T3 cells attached on rough surfaces produced by cathodic cage configuration was statistically significant p<0,05 compared to polished discs. CONCLUSIONS: Glow discharge plasma nitriding improve titanium surface roughness and wettability. MC3T3 cell adhesion behavior is related to substrate chemical composition and topography. Resumo: INTRODU??O: Superf?cies de tit?nio modificadas por diferentes m?todos foram estudadas com base nos par?metros f?sicos e qu?micos de caracteriza??o superficial e sua influ?ncia no comportamento de c?lulas pr?osteobl?sticas (MC3T3) in vitro. M?TODOS: Discos de tit?nio comercialmente puro grau II foram submetidos a tr?s m?todos de modifica??o de superf?cie (polimento, nitretados em plasma em configura??o planar e gaiola cat?dica). As diferentes superf?cies foram caracterizadas para observar o efeito do processamento na estrutura da camada superficial, na rugosidade e molhabilidade. Ensaios de ades?o e prolifera??o celular usando linhagens de c?lulas pr?-osteobl?sticas MC3T3 foram realizados para avaliar o efeito das novas superf?cies no comportamento celular in vitro. RESULTADOS: Os resultados demonstraram que a nitreta??o em plasma na configura??o de gaiola cat?dica produz superf?cies mais rugosas (p<0,02) e com menores ?ngulos de contato com a ?gua. CONCLUS?ES: A ades?o celular ? maior nas superf?cies mais rugosas do que nas superf?cies polidas (p<0,05) e reagem de modo diferente a composi??o qu?mica do substrato e ? topografia da superf?cie

Submitted by Alex Sandro R?go (alex@ifpb.edu.br) on 2017-06-06T12:00:22Z No. of bitstreams: 1 32- Mauricio Vicente - ESTUDO COMPUTACIONAL DE BIOCHIP EM FIBRA ?PTICA BASEADO NA RESSON?NCIA DE PL?SMONS DE SUPERF?CIE.pdf: 2764637 bytes, checksum: 32348af8b12e2c07b56a0ffc0014865a (MD5) / Approved for entry into archive by Alex Sandro R?go (alex@ifpb.edu.br) on 2017-06-06T12:04:04Z (GMT) No. of bitstreams: 1 32- Mauricio Vicente - ESTUDO COMPUTACIONAL DE BIOCHIP EM FIBRA ?PTICA BASEADO NA RESSON?NCIA DE PL?SMONS DE SUPERF?CIE.pdf: 2764637 bytes, checksum: 32348af8b12e2c07b56a0ffc0014865a (MD5) / Made available in DSpace on 2017-06-06T12:04:04Z (GMT). No. of bitstreams: 1 32- Mauricio Vicente - ESTUDO COMPUTACIONAL DE BIOCHIP EM FIBRA ?PTICA BASEADO NA RESSON?NCIA DE PL?SMONS DE SUPERF?CIE.pdf: 2764637 bytes, checksum: 32348af8b12e2c07b56a0ffc0014865a (MD5) Previous issue date: 2017-05-06 / O fen?meno de Resson?ncia de Pl?smons de Superf?cie (RPS) tem sido utilizado para a produ??o de biossensores por apresentar vantagens, como a possibilidade de miniaturiza??o, baixo custo, alta sensibilidade, alta seletividade e velocidade de resposta. Este trabalho tem como objetivo realizar um estudo computacional dos biossensores RPS em fibra ?ptica convencional (s?lica e/ou pol?mero). Neste caso, a casca da fibra ? removida na regi?o sensora, e o n?cleo ? recoberto por uma camada fina de metal, formando o biochip ?ptico. A an?lise computacional ? realizada com o aux?lio do software MATLAB, em que foi implementado o modelo de multicamadas de Fresnel, e o software COMSOL Multiphysics, baseado no m?todo dos elementos finitos, para a modelagem do biochip. O objetivo ? modificar e investigar os par?metros geom?tricos (dimens?es da fibra, espessura da camada met?lica) e eletromagn?ticos (comprimento de onda, ?ndice de refra??o, ?ngulo de incid?ncia, dentre outros) do biochip. Al?m disso, o tipo de metal utilizado ? de fundamental import?ncia, uma vez que sua oxida??o pode implicar na diminui??o do desempenho do biossensor, em contato com o analito (solu??o aquosa). O uso de metais nobres, como Ouro e Prata, na configura??o do biossensor oferece bons resultados, observados por meio dos valores de FWHM e refletividade, tanto para a ocorr?ncia do fen?meno RPS, quanto ao se analisar os par?metros de desempenho do biossensor. An?lises foram realizadas utilizando os modos de interroga??o angular e espectral. Por fim, tamb?m s?o consideradas configura??es de biochip com camadas de grafeno dispostas nas interfaces metal-fibra e metal-analito, com o fito de estudar a possibilidade de aumento da transmissividade do campo el?trico evanescente. Os resultados das an?lises s?o mostrados e discutidos.

Submitted by Caroline Xavier (caroline.xavier@pucrs.br) on 2017-05-25T14:55:38Z No. of bitstreams: 1 TES_JULIANA_DONCATTO_BETTINELLI_COMPLETO.pdf: 2435463 bytes, checksum: 80f6606ba1ecc5861b22ef0b6b9e87de (MD5) / Made available in DSpace on 2017-05-25T14:55:38Z (GMT). No. of bitstreams: 1 TES_JULIANA_DONCATTO_BETTINELLI_COMPLETO.pdf: 2435463 bytes, checksum: 80f6606ba1ecc5861b22ef0b6b9e87de (MD5) Previous issue date: 2017-01-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Since the 1970s, the application of the CAD / CAM technique has been suggested in the dental clinic with the aim of simplifying, automating and guaranteeing quality levels with micrometric adaptations of our dental prostheses4,5. The objective of this study was to evaluate the viability of the diode laser as an alternative to hydrofluoric acid and aluminum oxide blasting compared to the machining process in the surface treatment of the following restorative materials: hybrid ceramics, nanoceramic resin, reinforced lithium silicate ceramics by zirconia, feldspathic ceramics, vitreous ceramics with crystalline structure of lithium disilicate, vitreous ceramics with crystalline structure of leucite. A specimen was prepared for each of the seven CAD / CAM restorative materials; And, this was divided into four parts. The dimensions of the specimens were 1cm in length and 1cm in width, in the thickness of 1mm, color A2. The surface area of the specimen was subdivided into quadrants; (A) was applied to the diode laser, (B) received the blasting of aluminum oxide, (C) received the application of hydrofluoric acid (5 or 10%) or cleaning with isopropyl alcohol (10%); As indicated by the manufacturer, (D) no conditioning was applied and the surface passed through the machining process only. Images were taken at a 15,000- fold increase (SEM). For the surface roughness test, ten measurements were taken, with cut-off 0.25. Statistical data were submitted to ANOVA and Tukey's test. Knowing the need to seek new alternatives of a conditioning protocol on the surface of restorative materials in CAD / CAM, it was highly effective, fast, simplified and mainly safe. It can be stated that the use of the diode laser seems to be feasible for the surface treatment of ceramic with crystalline structure of lecithin Empress CAD and ceramic feldspathic Cerec Blocs CAD / CAM, as it produced changes in the surface of these ceramics studied. / Desde a d?cada de setenta, a aplica??o da t?cnica CAD/CAM vem sendo sugerida na cl?nica odontol?gica com o objetivo de simplificar, automatizar e garantir n?veis de qualidade com adapta??es microm?tricas das nossas pr?teses dent?rias4,5. O objetivo deste estudo foi avaliar a viabilidade do laser de diodo, como alternativa ao ?cido fluor?drico e jateamento com ?xido de alum?nio em compara??o ao processo de usinagem no tratamento superficial dos seguintes materiais restauradores: cer?mica h?brida, resina nanocer?mica, cer?mica de silicato de l?tio refor?ada por zirc?nia, cer?mica feldsp?tica, cer?mica v?trea com estrutura cristalina de dissilicato de l?tio, cer?mica v?trea com estrutura cristalina de leucita. Foi confeccionado um corpo de prova para cada um dos sete materiais restauradores em CAD/CAM; e, este foi dividido em quatro partes. As dimens?es dos corpos de prova foram de 1 cm de comprimento por 1cm de largura, na espessura de 1mm, cor A2. A ?rea de superf?cie do corpo-de-prova foi subdivida em quadrantes; de forma que em (A) foi aplicado o laser de diodo, (B) recebeu o jateamento de ?xido de alum?nio, (C) recebeu a aplica??o de ?cido fluor?drico (5 ou 10%) ou limpeza com ?lcool isoprop?lico (10%); conforme indica??o do fabricante, (D) n?o foi aplicado condicionamento e a superf?cie passou apenas pelo processo de usinagem. Foram realizadas imagens no aumento de 15.000 vezes (MEV). Para o teste de rugosidade superficial, foram tomadas dez medidas, com cut-off (valor de corte) 0,25. Os dados estat?sticos obtidos foram submetidos ? ANOVA e ao teste de Tukey. Sabendo-se da necessidade de buscar novas alternativas de um protocolo de condicionamento na superf?cie dos materiais restauradores em CAD/CAM, que fosse altamente eficaz, r?pido, simplificado e principalmente seguro. Pode-se afirmar que a utiliza??o do laser de diodo parece ser vi?vel para o tratamento superficial da cer?mica v?trea com estrutura cristalina de leucita Empress CAD e cer?mica feldsp?tica Cerec Blocs CAD/CAM, pois produziu altera??es na superf?cie destas cer?micas estudadas.

Submitted by Jadson Francisco de Jesus SILVA (jadson@uefs.br) on 2018-02-21T22:38:38Z No. of bitstreams: 1 NEIVASuriaSeixas_ DESENHOS_DE_COURO.pdf: 272955981 bytes, checksum: b4858840e27db87b17f281c3e9fda9f2 (MD5) / Made available in DSpace on 2018-02-21T22:38:38Z (GMT). No. of bitstreams: 1 NEIVASuriaSeixas_ DESENHOS_DE_COURO.pdf: 272955981 bytes, checksum: b4858840e27db87b17f281c3e9fda9f2 (MD5) Previous issue date: 2017-02-17 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This dissertation presents the designs made from leather used by the brazilian northeast?s country cowboys: a type of surface design which can become a working gear but also a fashionable apparel. This tooled and carved leather has been made by generations in the sert?o and it is named here after ?desenho de couro?. The purpose of this work is to approach the manifestations of such ornamental leather technics considering their regional and historical presented aspects, or preexistences of its present form. The aesthetic and technical connections among the different expressions of the leather designs are understood through the survival dialetics of the images in the collective memory. This comes to be the art history methodology proposed by Warburg. Considering that is still possible to contact living masters of leather in activity, and to collect information regarding the technique transmission, this resource was used in order to understand the aesthetics and uses of that leather culture, which has its ancestral in the iberic-mudeyyan leatherwork. Both the documents of leather trade in the century XVIII, as well as of the history of the livestock in the Brazilian countryside corroborates with the hypotheses regarding the influence of the Iberian leather culture and its productive main points, today, in the Bahia countryside around Feira de Santana and Ipir?, as in Cariri from Pernambuco. The study of this leather design, more than a record of a Brazilian visual production, it is an living document of the national leather traditions and an outstanding element in the construction of the cowboy's identity as well as of an economic production and material culture of Brazil. / Esta disserta??o apresenta o desenho do encouramento dos vaqueiros sertanejos do nordeste: um desenho de superf?cie, de natureza indument?ria, ora laboral, ora social, que ? produzido h? gera??es nos sert?es, denominado aqui como desenho de couro. O objetivo deste trabalho ? abordar as manifesta??es desse desenho ornamental em superf?cie de couro por seus aspectos regionais, presentes, e hist?ricos, ou preexistentes. As conex?es est?ticas e t?cnicas, entre as diferentes express?es dos desenhos de couro, consideraram a dial?tica da sobreviv?ncia das imagens na mem?ria coletiva ? isto ?, a metodologia de hist?ria da arte proposta por Warburg. Foi lan?ado m?o do recurso, ainda poss?vel, de contatar mestres do couro em atividade e coletar informa??es a respeito da transmiss?o das t?cnicas, est?ticas e usos dessa cultura coureira, que tem seus ancestrais na corioplastia ?bero-mudej?r. O respaldo documental de tr?nsitos comerciais de couro no s?culo XVIII bem como da hist?ria da pecu?ria no sert?o brasileiro, corrobora com as hip?teses aventadas a respeito da influ?ncia da corioplastia ib?rica e da sua concentra??o produtiva, hoje, no sert?o baiano dos arredores de Feira de Santana e Ipir?, como no Cariri pernambucano. O estudo do desenho de couro, para al?m do registro de uma produ??o visual brasileira, ? um documento vivo da corioplastia nacional e um elemento marcante na constru??o n?o apenas da identidade do vaqueiro como da produ??o da economia e da cultura material do Brasil.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-12-01T23:34:01Z No. of bitstreams: 1 SergioRobertoDantas_DISSERT.pdf: 1439747 bytes, checksum: d876ed77a5433b952a46ba0482101886 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-12-05T21:54:18Z (GMT) No. of bitstreams: 1 SergioRobertoDantas_DISSERT.pdf: 1439747 bytes, checksum: d876ed77a5433b952a46ba0482101886 (MD5) / Made available in DSpace on 2017-12-05T21:54:18Z (GMT). No. of bitstreams: 1 SergioRobertoDantas_DISSERT.pdf: 1439747 bytes, checksum: d876ed77a5433b952a46ba0482101886 (MD5) Previous issue date: 2017-09-06 / Frequentemente, as superf?cies seletivas em frequ?ncias t?m recebido grande aten??o por parte de pesquisadores em todo o mundo, devido a suas caracter?sticas de filtragem. Essas estruturas evolu?ram em termos de fabrica??o. Assim, as geometrias utilizadas, inicialmente, eram fios em forma de grade e, posteriormente, diversos tipos de novas geometrias tais como: elementos retangulares, quadrados, circulares, an?is, espiras quadradas etc., foram utilizadas. Recentemente, elementos fractais e, mais recentemente, multifractais foram empregados. O emprego dessas geometrias ? feito com o objetivo de se obter uma resposta em frequ?ncia espec?fica. Par?metros importantes, como largura de banda e frequ?ncia de resson?ncia s?o atendidos a partir do emprego de uma determinada geometria. Neste trabalho ? proposta uma geometria multifractal com dupla similaridade, que tem como caracter?stica principal baixa sensibilidade ? varia??o da periodicidade com rela??o ? frequ?ncia de resson?ncia. Essa especificidade trar? ganho no que diz respeito ? elabora??o do projeto, pois o controle da largura de banda ? feito apenas com a altera??o da periodicidade. Diversas simula??es foram implementadas no Ansoft Designer e para efeito de comprova??o, estruturas tradicionais foram simuladas, visando ? demonstra??o do efeito da periodicidade na frequ?ncia de resson?ncia e, em seguida, dois prot?tipos foram constru?dos e feitas medi??es para validar experimentalmente as simula??es. / Often, frequency selective surfaces have received wide attention from researchers around the world because of their filtering characteristics. These structures evolved in terms of manufacturing. Thus, the geometries used, initially, were yarns in the form of a grid and later, several types of new geometries such as: rectangular, square, circular, rings, square, etc., were used. Recently, fractals and, more recently, multifractal elements have been employed. The use of these geometries is done in order to obtain a specific frequency response. Important parameters such as bandwidth and resonant frequency are met from the use of a given geometry. In this work a multifractal geometry with double similarity is proposed, which has as main characteristic low sensitivity to the variation of the periodicity with respect to the resonance frequency. This specificity will bring gain with regard to the elaboration of the project, because the control of the bandwidth is made only with the change of periodicity. Several simulations were implemented in Ansoft Designer and for the purpose of proving, traditional structures were simulated, aiming at demonstrating the effect of periodicity on the resonance frequency, and then two prototypes were constructed and measurements were taken to experimentally validate the simulations.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-01-15T21:40:48Z No. of bitstreams: 1 MarcionilaNeliLimaDosSantos_DISSERT.pdf: 11845216 bytes, checksum: ab27f3a37cb03e804ce73ec2471f74c4 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-01-17T14:20:49Z (GMT) No. of bitstreams: 1 MarcionilaNeliLimaDosSantos_DISSERT.pdf: 11845216 bytes, checksum: ab27f3a37cb03e804ce73ec2471f74c4 (MD5) / Made available in DSpace on 2018-01-17T14:20:49Z (GMT). No. of bitstreams: 1 MarcionilaNeliLimaDosSantos_DISSERT.pdf: 11845216 bytes, checksum: ab27f3a37cb03e804ce73ec2471f74c4 (MD5) Previous issue date: 2017-08-14 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O estudo da s?ntese e propriedades das nanopart?culas superparamagn?ticas vem crescendo de forma significativa ao longo dos anos, abrangendo diversas ?reas do conhecimento. A aditiva??o de nanopart?culas em ?leos minerais ? uma delas, para atuarem como lubrificantes, apresentando propriedades tribol?gicas superiores aos comerciais, reduzindo o coeficiente de atrito e o desgaste dos elementos mec?nicos. Essa disserta??o tem como finalidade estudar o desempenho tribol?gico de um ?leo mineral paraf?nico aditivado com nanopart?culas superparamagn?ticas de ?xido de ferro (SPIONs), a fim de avaliar a influ?ncia da inser??o das nanopart?culas nas propriedades tribol?gicas (coeficiente de atrito e desgaste) quando submetido a diferentes cargas e rugosidades da superf?cie. Os ensaios foram realizados no trib?metro SRV-4. Os par?metros analisados foram tamanhos de nanopart?culas (2 nm, 7 nm e 15 nm), a rugosidade da superf?cie, sendo esta polida, lixada e retificada, e a carga aplicada, no modo rampa de 30N a 250N. A caracteriza??o das nanopart?culas foi realizada por Difra??o de Raio X (DRX), Microscopia Eletr?nia de Transmiss?o (MET) e Sistema de Medidas das Propriedades F?sicas (PPMS). Quanto a caracteriza??o do ?leo puro e do ferrofluido, foi realizada por FTIR. Os valores de coeficiente de atrito foram adquiridos durante o ensaio na SRV-4. J? o desgaste da esfera AISI 52100 e do disco AISI H13 foram analisados por Microscopia Eletr?nica de Varredura (MEV), Espectroscopia Raman e Interferometria de Luz Branca, tendo como refer?ncia os resultados obtidos para o ?leo puro. As SPIONs tiveram suas melhores performances como aditivo antifric??o em baixas cargas (30N e 50N) e em superf?cie rugosa (Retificada). / The study of the synthesis and properties of superparamagnetic nanoparticles is growing up significantly along the years, allowing interaction with many knowledge. The nanoparticles additivation in mineral oils is one of them, for its performance as lubrificants, showing superior tribological properties in comparison with commercial ones, reducing the fricction coefficient and the wear of the mechanical components. The purpose of this research is to study the tribological performance of a parafinic mineral oil additived with superparamagnetic iron oxide nanoparticles (SPIONs), in order to evaluate the influence of the nanoparticles in the tribological properties (fricction coefficient and wear) when exposed to different loads and surface roughnesses differents. The tribotests were using a SRV-4 tribometer. The test parameters were the nanoparticle size (2 nm, 7 nm e 15 nm), the surface roughness, in this case polished, sanded and grounded, and the applied load steps over time (30 N ? 250 N). The nanoparticles characterization was conducted by X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM) and Physical Property Measurement System (PPMS). About the pure oil characterization and the ferroluids, was performed by FTIR. The fricction coefficient values were obtained during the tribotest in the SRV-4 tribometer. Furthermore, the AISI 52100 stell ball wear and AISI H13 steel disc wear were analyzed by Scanning Electron Microscopy (SEM), Raman Spectroscopy and White Light Interferometry (WLI), using the results obtained for pure oil as references. The SPIONs best results as antifriction additive were obtained with the low loads (30 N and 50 N) and with the rough surface (Ground).

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-07T12:40:01Z No. of bitstreams: 2 joao_gabriel_guimares_luz.pdf: 5854830 bytes, checksum: 3b384576c220fecec7431b8c107b9ea1 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-07T12:40:28Z (GMT) No. of bitstreams: 2 joao_gabriel_guimares_luz.pdf: 5854830 bytes, checksum: 3b384576c220fecec7431b8c107b9ea1 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-07T12:40:47Z (GMT) No. of bitstreams: 2 joao_gabriel_guimares_luz.pdf: 5854830 bytes, checksum: 3b384576c220fecec7431b8c107b9ea1 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-01-07T12:40:47Z (GMT). No. of bitstreams: 2 joao_gabriel_guimares_luz.pdf: 5854830 bytes, checksum: 3b384576c220fecec7431b8c107b9ea1 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2014 / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / O objetivo desse trabalho foi desenvolver um imunosensor fundamentado em espectroscopia de resson?ncia de pl?smons de superf?cie (SPR) para a detec??o de anticorpos anti-Trypanosoma cruzi por meio da imobiliza??o de ant?genos brutos do parasito em monocamadas auto-organizadas mistas (SAMs) formadas por ?cido 11-mercaptoundecan?ico (11-MUA) e ?cido 3-mercaptopropi?nico (3-MPA). Na sua constru??o, as concentra??es de cada alcanotiol, bem como a propor??o de cada um deles na composi??o das SAMs mistas foram definidas atrav?s de c?lculos de cobertura de superf?cie Posteriormente, o ant?geno em diferentes concentra??es foi imobilizado sobre as SAMs mistas ativadas constru?das em disco de ouro e, a varia??o do ?ngulo de SPR (??SPR) decorrente dessa etapa foi monitorada em tempo real. O imunosensor desenvolvido foi ent?o eletroquimicamente caracterizado por voltametria c?clica (VC) e espectroscopia de imped?ncia eletroqu?mica (EIE) e as respostas do mesmo frente a um pool de soros de indiv?duos infectados e n?o infectados pelo T. cruzi foram avaliadas. Em seguida, foram determinados par?metros para otimiza??o do dispositivo, incluindo tempo de imobiliza??o e concentra??o do ant?geno, bloqueio e regenera??o da superf?cie sensora e tempo envolvido nas an?lises do imunoensaio. Por fim, a dilui??o de trabalho do soro e o ponto de corte do imunosensaio foram escolhidos e os ?ndices de desempenho do mesmo como t?cnica para o imunodiagn?stico da DC foram calculados empregando 171 soros, sendo 99 de indiv?duos infectados pelo T. cruzi, 30 de n?o infectados e 42 portadores de outras infec??es. Os maiores valores de foram encontrados para a concentra??o 1.0 mmol L-1 de 11-MUA e 3-MPA e para as SAMs mistas formadas na propor??o de 1:10. A imobiliza??o dos ant?genos foi conduzida com ?xito, de modo que houve linearidade entre a concentra??o utilizada e o ??SPR. Por VC e EIE, foi demonstrado que a liga??o covalente das prote?nas aumentou a resist?ncia ? transfer?ncia eletr?nica quando comparado com o comportamento da sonda eletroqu?mica sobre as SAMs mistas. Ademais, o imunosensor se mostrou, semelhante ao ELISA, capaz de detectar anticorpos anti-T. cruzi em amostras de soros dilu?das at? 1280 vezes. J? em rela??o aos soros negativos, o dispositivo detectou ??SPR significativas apenas at? a dilui??o 1:160, o que demonstrou razo?vel capacidade do m?todo em distinguir indiv?duos infectados de n?o infectados. Dentre os par?metros anal?ticos avaliados ficou definido o tempo de imobiliza??o e a concentra??o do ant?geno em 20 minutos e 30 ?g mL-1, o bloqueio da superf?cie por 10 minutos com solu??o de BSA 1.0%, a regenera??o pela inje??o de SDS 1.0% e o tempo de avalia??o da resposta do dispositivo em 20 minutos. A dilui??o do soro em 1:320 e o ponto de corte a 17.2 m? foram os crit?rios metodol?gicos definidos para o imunosensaio. Os ?ndices de desempenho calculados determinaram um excelente potencial do imunosensor no diagn?stico sorol?gico da doen?a de Chagas, com valores de sensibilidade de 100%, especificidade de 97.2%, valor preditivo positivo igual a 98%, valor preditivo negativo de 100% e acur?cia global estimada em 99.6%. O n?mero de rea??es cruzadas em soros de indiv?duos com leishmaniose visceral foi menor no m?todo proposto (1/7) do que no ELISA (6/7). A partir dos resultados ? poss?vel afirmar que foi desenvolvido com ?xito um imunosensor sens?vel, espec?fico, r?pido, simples e de f?cil execu??o para o diagn?stico sorol?gico da doen?a de Chagas. / Disserta??o (Metrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2014. / ABSTRACT The aim of the present study was to describe the development of an unpublished SPR-based immunosensor for detection of anti-T. cruzi antibodies in human serum through the covalently immobilized T. cruzi antigen on a mixed SAM of 11-mercaptoundecanoic acid (11-MUA) and 3-mercaptopropionic acid (3-MPA) on a SPR sensor chip. In its construction, the concentrations of each alkanethiol, and the proportion of each in the composition of the mixed SAMs were defined by calculation of surface coverage Subsequently, the antigen at different concentrations was immobilized on the activated mixed SAMs built on gold disc, and the change in the SPR angle (??SPR) resulting from this step was monitored in real time. The imunosensor developed was electrochemically characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), and the responses of a pool of sera from individuals infected and not infected with T. cruzi were also evaluated. Then, some parameters for the optimization of the device were defined, including immobilization time and antigen concentration, blocking and regeneration of the sensor surface and time involved in the analysis of the immunoassay. Finally, the working dilution of the serum and the cut-off point were chosen and the performance indices of the technique were calculated using 171 sera - 99 individuals infected with T. cruzi, 30 not infected and 42 with other infections. The highest values ??of ? were found for the 1.0 mmol L-1 concentration of 11-MUA and 3-MPA and mixed SAMs formed at 1:10 proportion. The immobilization of antigens was conducted successfully, so there was linearity between the concentration used and ??SPR. For VC and EIS has been demonstrated that covalent attachment of the protein increased the electron transfer resistance when compared with the behavior of the electrochemical probe on the mixed SAMs. Furthermore, imunosensor showed, similarity to ELISA, capable of detecting anti-T. cruzi in sera diluted up 1280 times. In relation to the negative sera, the device detected significant ??SPR only until dilution 1:160, which demonstrated the ability of the method to distinguish infected from uninfected individuals. The following analytical parameters were defined: time of immobilization and antigen concentration at 20 minutes and 30 mg mL-1, blocking surface for 10 minutes with 1.0% BSA solution, regeneration by injection of 1.0% SDS and time evaluation of the response of the device in 20 minutes. The serum dilution at 1:320 and cut-off point at 17.2 m? were the methodological criteria for immunoassay. The performance indices calculated for the imunosensor demonstrated a great potential in serological diagnosis of Chagas' disease, with a sensitivity of 100%, specificity of 97.2%, positive predictive value of 98%, negative predictive value of 100% and global accuracy estimated in 99.6%. The number of cross-reactivity with sera from individuals with visceral leishmaniasis was lower (1/7) than in ELISA (6/7). Based on the results, it is possible to stay that a sensitive, specific, rapid, simple and easy immunosensor was successfully with potential application at serological diagnosis of Chagas disease.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2015-12-14T21:36:46Z No. of bitstreams: 1 AlbanisaFelipeDosSantos_DISSERT.pdf: 4703287 bytes, checksum: e882be5f3bf32915ca0f8f6710487c3d (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2015-12-16T17:33:34Z (GMT) No. of bitstreams: 1 AlbanisaFelipeDosSantos_DISSERT.pdf: 4703287 bytes, checksum: e882be5f3bf32915ca0f8f6710487c3d (MD5) / Made available in DSpace on 2015-12-16T17:33:34Z (GMT). No. of bitstreams: 1 AlbanisaFelipeDosSantos_DISSERT.pdf: 4703287 bytes, checksum: e882be5f3bf32915ca0f8f6710487c3d (MD5) Previous issue date: 2013-07-25 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Neste trabalho, as propriedades de auto-similaridade dos fractais s?o exploradas para o desenvolvimento de superf?cies seletivas de frequ?ncia (Frequency Selectives Surfaces - FSS) com v?rias bandas de rejei??o. Em particular, s?o considerados fractais de Gosper, na defini??o dos formatos dos elementos das FSS. Por conta da dificuldade de impress?o de detalhes dos elementos das FSS, s?o considerados apenas elementos pr?-fractais, com at? tr?s itera??es fractais. As simula??es foram realizadas com o uso do programa comercial Ansoft Designer. Para fins de valida??o de resultados, foram constru?dos v?rios prot?tipos de FSS com elementos pr?-fractais. No processo de fabrica??o, os formatos dos elementos pr?-fractais foram desenhados com aux?lio do programa Corel Draw. Os prot?tipos constru?dos foram medidos atrav?s de um analisador de redes vetorial (modelo N3250A, da Agilent Technologies). A utiliza??o de elementos pr?-fractais nas estruturas de FSS consideradas, permitiu verificar que o aumento do n?vel fractal possibilita a redu??o do tamanho dos elementos, por?m reduz a largura de banda das mesmas. Neste sentido, ? tamb?m investigado o efeito produzido pelo cascateamento de estruturas de FSS na largura de banda. Foi observado que o uso de estruturas cascateadas, al?m de aumentar a largura de banda, permitiu, em alguns casos, a obten??o de at? respostas em frequ?ncia com tr?s bandas de opera??o entre 6 GHz e 15 GHz. / The fractal self-similarity property is studied to develop frequency selective surfaces (FSS) with several rejection bands. Particularly, Gosper fractal curves are used to define the shapes of the FSS elements. Due to the difficulty of making the FSS element details, the analysis is developed for elements with up to three fractal levels. The simulation was carried out using Ansoft Designer software. For results validation, several FSS prototypes with fractal elements were fabricated. In the fabrication process, fractals elements were designed using computer aided design (CAD) tools. The prototypes were measured using a network analyzer (N3250A model, Agilent Technologies). Matlab software was used to generate compare measured and simulated results. The use of fractal elements in the FSS structures showed that the use of high fractal levels can reduce the size of the elements, at the same time as decreases the bandwidth. We also investigated the effect produced by cascading FSS structures. The considered cascaded structures are composed of two FSSs separated by a dielectric layer, which distance is varied to determine the effect produced on the bandwidth of the coupled geometry. Particularly, two FSS structures were coupled through dielectric layers of air and fiberglass. For comparison of results, we designed, fabricated and measured several prototypes of FSS on isolated and coupled structures. Agreement was observed between simulated and measured results. It was also observed that the use of cascaded FSS structures increases the FSSs bandwidths and, in particular cases, the number of resonant frequencies, in the considered frequency range. In future works, we will investigate the effects of using different types of fractal elements, in isolated, multilayer and coupled FSS structures for applications on planar filters, high-gain microstrip antennas and microwave absorbers

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-05T17:58:44Z No. of bitstreams: 1 EricoCadineliBraz_TESE.pdf: 8760899 bytes, checksum: 8aab2fdf5e45acdc3b8815b487ca5319 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-01-11T18:20:07Z (GMT) No. of bitstreams: 1 EricoCadineliBraz_TESE.pdf: 8760899 bytes, checksum: 8aab2fdf5e45acdc3b8815b487ca5319 (MD5) / Made available in DSpace on 2016-01-11T18:20:07Z (GMT). No. of bitstreams: 1 EricoCadineliBraz_TESE.pdf: 8760899 bytes, checksum: 8aab2fdf5e45acdc3b8815b487ca5319 (MD5) Previous issue date: 2014-09-25 / As superf?cies seletivas em frequ?ncia s?o estruturas cada vez mais comuns em sistemas de telecomunica??es por apresentarem vantagens geom?tricas e eletromagn?ticas. J? as superf?cies seletivas em frequ?ncia com geometria do tipo fractal permitiram uma redu??o, ainda maior, no comprimento el?trico proporcionando uma maior flexibilidade no projeto dessas estruturas. Neste trabalho, ? investigado o uso das geometrias multifractais nas superf?cies seletivas em frequ?ncia. Foram propostas e analisadas tr?s estruturas com diferentes geometrias multifractais. A primeira estrutura permitiu projetar estruturas multibanda com maior flexibilidade no controle das frequ?ncias de resson?ncias e largura de banda. J? a segunda estrutura proporcionou um aumento de largura de banda mesmo com o aumento do n?vel fractal. A terceira estrutura apresentou resposta com estabilidade angular, dupla polariza??o e permitiu um aumento de largura de banda com o crescimento da mulfractalidade da estrutura. Al?m disso, as estruturas propostas aumentaram o grau de liberdade nos projetos multibanda, visto que possuem m?ltiplas propor??es de frequ?ncias de resson?ncias entre as bandas adjacentes, e s?o f?ceis de ser implementadas. A valida??o das estruturas propostas foi verificada, inicialmente, atrav?s de simula??es realizadas no software Ansoft Designer e, em seguida, foram constru?das as estruturas e obtidos os resultados experimentais / Frequency Selective surfaces are increasingly common structures in telecommunication systems due to their geometric and electromagnetic advantages. As a matter of fact, the frequency selective surfaces with fractal geometry type would allow an even bigger reduction of the electrical length which provided greater flexibility in the design of these structures. In this work, we investigated the use of multifractal geometry in frequency selective surfaces. Three structures with different multifractal geometries have been proposed and analyzed. The first structure allowed the design of multiband structures with greater flexibility in controlling the resonant frequencies and bandwidth. The second structure provided a bandwidth increase even with the rising of the fractal level. The third structure showed response with angle stability, dual polarization and provided room for a bandwidth increase with the rising of the structural multifractality. Furthermore, the proposed structures increased the degree of freedom in the multiband designs because they have multiple resonant frequencies ratios between adjacent bands and are easy to deploy. The validation of the proposed structures was initially verified through simulations in Ansoft Designer software and then the structures were constructed and the experimental results obtained

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-01-26T19:01:21Z No. of bitstreams: 1 JussierDeOliveiraVitoriano_DISSERT.pdf: 29322891 bytes, checksum: 0c0254774bc22cc8cdd1189f3829e271 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-01-28T21:34:07Z (GMT) No. of bitstreams: 1 JussierDeOliveiraVitoriano_DISSERT.pdf: 29322891 bytes, checksum: 0c0254774bc22cc8cdd1189f3829e271 (MD5) / Made available in DSpace on 2016-01-28T21:34:07Z (GMT). No. of bitstreams: 1 JussierDeOliveiraVitoriano_DISSERT.pdf: 29322891 bytes, checksum: 0c0254774bc22cc8cdd1189f3829e271 (MD5) Previous issue date: 2015-01-09 / O presente trabalho teve como objetivo analisar a resposta biol?gica em superf?cies de tit?nio modificadas por plasma de Ar+N2+H2. Discos de tit?nio grau II receberam diferentes tratamentos de superf?cie com plasma de Ar+N2+H2, constituindo sete grupos experimentais incluindo amostras apenas polidas utilizadas como padr?o. Antes e ap?s o tratamento as amostras foram caracterizadas quanto ? topografia, estrutura cristalina e molhabilidade, usando microscopia de for?a at?mica, difra??o de raios-X, espectroscopia Raman e ensaio da gota s?ssil, respectivamente. Plasma sangu?neo rico em plaquetas (PRP) foi aplicado sobre as superf?cies modificadas em placas de cultivo. Imagens obtidas por microscopia eletr?nica das plaquetas aderidas foram analisadas a fim de verificar o comportamento das plaquetas nas diferentes condi??es experimentais. Verificou-se que a adi??o de H2 na atmosfera do plasma resultou em superf?cies mais rugosas, com picos arredondados. Essas superf?cies, ao contr?rio daquelas tratadas com atmosfera de alta concentra??o de N2, s?o menos propensas ? agrega??o plaquet?ria e, consequentemente, ? forma??o de trombos quando aplicadas em dispositivos biom?dicos. / This study aimed to analyze the biological response of titanium surfaces modified by plasma Ar + N2 + H2. Titanium disks grade II received different surface treatments Ar + N2 + H2 plasma, constituting seven groups including only polished samples used as standard. Before and after treatment the samples were evaluated in terms of topography, crystal structure and wettability, using atomic force microscopy, X-ray diffraction, Raman spectroscopy and testing of the sessile drop, respectively. Rich plasma (PRP) was applied to the surfaces modified in culture plates. Images obtained by scanning electron microscopy of the adhered platelets were analyzed to verify the behavior of platelets in the different experimental conditions. We verified that the adition of H2 on plasma atmosphere resulted in more rough surfaces, with round tops. These surfaces, in contrast to that surfaces treated with high concentration of N2, are less propense to platelet aggregation and, consequently, to the formation of thrombus when applied in biomedical devices.