Master of Science / Food Science - Food, Nutrition, Dietetics and Health / Kadri Koppel / Understanding the physiological activation and genetic variation of the sweet taste receptor (T1R) can improve formula optimization for products intended for a population of genetically diverse people. Computer modeling and cell culture techniques have thoroughly described the structure and binding sites of the T1R. The structure contains two subunits (T1R2 and T1R3) with multiple domains where sweet molecules can interact. The interaction takes place between individual molecules and amino acid residues of the T1R. The residues with which individual molecules interact differs between sweeteners. Person-to-person differences in the residue sequence of the T1R can arise from variation in the genes that encode the T1R (TAS1R), potentially effecting the function of the receptor. As a result of the specificity of binding interactions, genetic variation may affect sensitivity to some sweeteners, while sensitivity to other sweeteners remains normal. Therefore, it can be hypothesized that the level of person-to-person sweetness sensitivity variation may differ for each sweetener depending on the binding site of the molecule and site of T1R variation. The T1R structure, binding sites, and genetic variation will be reviewed, as well as potential parameters to predict the degree of sensitivity variation and formulation strategies to minimize the effects of sensitivity variation.

Starch residue samples from two Australian sweet potato varieties (Beauregard and Northern Star) and two Indonesian sweet potato varieties (Bis192 and Bis183), and commercial sample of sweet potato starch residue, were studied for their pectins. Pectins were extracted using 0.1M HCl, 0.05M NaOH, 0.1M HCl/0.75%SHMP, and 0.05M NaOH/0.75% SHMP. Hydrolysis of residual starch in the cell wall of sweet potato using heat stable α-amylase and amyloglucosidase was employed prior to pectin extraction to eliminate starch contamination. Pectins were characterised for yield, moisture, ash, galacturonic acid content (GA), degree of esterification (DE), neutral sugars content and molecular weight (MW). Selected pectins were also characterised for their dispersion and gel properties. Pectin with the highest yield was compared to commercial low methoxyl pectin (GRINSTED??YF 450) for its application in milk pudding. The pectin characteristics are dependent on variety and extraction process; however, the extraction methods were dominant. Yields were between 7 and 30 % of the cell wall. Ash varied from 2.9 to 6.8%. NaOH/SHMP extraction gave higher yields and ash contents. Moisture was between 5.5 and 6.7. GA varied from 27 to 80% with the highest found in Bis192 extracted using NaOH/SHMP. DE varied between traceable and 57%. HCl extraction gave higher DE, while NaOH/SHMP caused demethylation. Neutral sugars varied from 10 to 25% where galactose was the predominant sugar, followed by arabinose, rhamnose, glucose and xylose. Pectin MW was very heterogeneous. HCl extraction gave higher MW than the others. Dispersion of sweet potato pectins displayed shear-thinning. Consistency coefficient (K) increased, while flow behaviour index (n) decreased with increasing pectin concentration. HCl-extracted pectins formed stiff sugar-gels at concentrations higher than 2%. The optimum Ca2+ concentration for NaOH/SHMP-extracted pectin gels was 20 mg/g pectin. Pectin with the highest yield and stiffness (Norther Star extracted using NaOH containing SHMP) was used for milk pudding preparation. This pudding had comparable texture to pudding prepared from commercial low methoxyl citrus pectin (GRINSTED??YF 450). Overall, this study demonstrated that pectin from sweet potato starch residue is mainly low methoxyl pectin with possible chemical structure of rhamnogalacturonan I (RGI) that has potential for food applications.

Sweet corn is classified among highly perishable horticultural commodities. Thus, it can be deteriorated rapidly after harvest resulting in high loss and poorer produce quality. Sweet corn's sugar loss is about four times higher at 10°C compared to 0°C. Precooling, immediately after harvest, has shown to be an effective method to maintain the quality for a wide range of fresh fruits and vegetables during storage. Further, this method leads to reduction in metabolism and respiration rate of the produce, retardation of its senescence, and inhibition of growth of pathogens. In addition to diminishing postharvest losses of the produce, efficient precooling is required for increasing the length of duration of marketing time for better profitability. / Precooling of sweet corn was accomplished by three main methods including forced-air, water and vacuum cooling. Operating parameters such as temperature, pressure, orientation of corn cobs, air flow rate and water flow pattern were defined and studied for optimization. The assessment and comparison of the performance of precooling systems was achieved by determining the effect of these parameters on half cooling time and quality of the produce during storage for 7 and 21 days at 1°C and 90-95% RH. In addition, room cooling method was also tested and compared to the three precooling systems. The use of three sweet corn cultivars was important to compare their quality response to different cooling methods. / Experiments were performed on a lab-scale vacuum cooler and modified forced-air and water cooler systems. The results showed that changing the cob orientation perpendicular to the direction of flow medium, using higher air flow rate in forced-air cooling and immersed water flow pattern in water cooling, can significantly reduce the half cooling time of the produce. Finally, the best method to be recommended for precooling sweet corn is by using hydrocooling which results in superior quality produce and minimum time.

Starch residue samples from two Australian sweet potato varieties (Beauregard and Northern Star) and two Indonesian sweet potato varieties (Bis192 and Bis183), and commercial sample of sweet potato starch residue, were studied for their pectins. Pectins were extracted using 0.1M HCl, 0.05M NaOH, 0.1M HCl/0.75%SHMP, and 0.05M NaOH/0.75% SHMP. Hydrolysis of residual starch in the cell wall of sweet potato using heat stable α-amylase and amyloglucosidase was employed prior to pectin extraction to eliminate starch contamination. Pectins were characterised for yield, moisture, ash, galacturonic acid content (GA), degree of esterification (DE), neutral sugars content and molecular weight (MW). Selected pectins were also characterised for their dispersion and gel properties. Pectin with the highest yield was compared to commercial low methoxyl pectin (GRINSTED??YF 450) for its application in milk pudding. The pectin characteristics are dependent on variety and extraction process; however, the extraction methods were dominant. Yields were between 7 and 30 % of the cell wall. Ash varied from 2.9 to 6.8%. NaOH/SHMP extraction gave higher yields and ash contents. Moisture was between 5.5 and 6.7. GA varied from 27 to 80% with the highest found in Bis192 extracted using NaOH/SHMP. DE varied between traceable and 57%. HCl extraction gave higher DE, while NaOH/SHMP caused demethylation. Neutral sugars varied from 10 to 25% where galactose was the predominant sugar, followed by arabinose, rhamnose, glucose and xylose. Pectin MW was very heterogeneous. HCl extraction gave higher MW than the others. Dispersion of sweet potato pectins displayed shear-thinning. Consistency coefficient (K) increased, while flow behaviour index (n) decreased with increasing pectin concentration. HCl-extracted pectins formed stiff sugar-gels at concentrations higher than 2%. The optimum Ca2+ concentration for NaOH/SHMP-extracted pectin gels was 20 mg/g pectin. Pectin with the highest yield and stiffness (Norther Star extracted using NaOH containing SHMP) was used for milk pudding preparation. This pudding had comparable texture to pudding prepared from commercial low methoxyl citrus pectin (GRINSTED??YF 450). Overall, this study demonstrated that pectin from sweet potato starch residue is mainly low methoxyl pectin with possible chemical structure of rhamnogalacturonan I (RGI) that has potential for food applications.

Thesis (M.S.)--Michigan State University. Dept. of Horticulture, 2008. / Title from PDF t.p. (viewed on July 28, 2009) Includes bibliographical references. Also issued in print.

Vitamin A deficiency has been recognised as a widespread problem affecting about 750 million people, mostly in developing countries. Mortality due to vitamin A deficiency can be reduced by as much as 23% with improved vitamin A status. Vitamin A's immuneenhancing aspects strengthen the body's defence system against infectious diseases such as measles, malaria and diarrhoea, thus preventing death. Children beyond the weaning age (6 months to 6 years) are most at risk. In South Africa, one in three children has a low vitamin A status, with the rural areas being most affected. Orange-fleshed sweet potato (OFSP) has emerged as a promising plant source with a high beta-carotene content that can make a significant contribution to the vitamin A intake of individuals at risk of vitamin A deficiency. The purpose of this study was to determine the nutrient and sensory quality of OFSP. To this end, the nutrient content of different cultivars of OFSP was determined as well as the sensory characteristics and consumer acceptability was established. During the first phase, four different cultivars of OFSP i.e. Resisto, W119, Jewel and A 15, plus one composite sample, all cultivated by the Agricultural Research Council (ARC)-Roodeplaat, South Africa, were sampled for nutrient analysis. During the second phase (descriptive sensory analysis), four OFSP cultivars and one white-fleshed sweet potato (WFSP) cultivar i.e. Blesbok, were evaluated. A trained sensory panel was used to establish terminology for describing the sensory attributes of the different sweet potato cultivars in terms of its aroma, texture, flavour and aftertaste attributes. Consumer preference (n=180) for OFSP and WFSP was measured by means of a paired preference test. Focus group discussions were conducted to verify findings of the consumer preference test. The results obtained from the nutrient analysis confirmed that OFSP is an excellent source of beta-carotene. A 100 g portion of cooked OFSP can provide up to 6528 ~g beta-carotene, which is approximately 136% of the RDA for vitamin A for children four to eight years. High levels of other nutrients present in OFSP were identified namely vitamin C, calcium and zinc. OFSP further contributes 28% vitamin C, 13% calcium, 15% magnesium and 75.6% zinc of their daily requirements. This study confirms the valuable contribution that OFSP can make as a food-based approach to reduce vitamin A deficiency in individuals at risk. The sensory profiles indicated that OFSP differed from WFSP in colour, flavour and texture. OFSP is generally less moist and subsequently more dense and adhesive compared to WFSP. The latter being more fibrous and less firm than OFSP. Overall OFSP had and earthy aroma which was not typical of WFSP and had a sweeter flavour. The flavour of OFSP was described as similar to that of yellow vegetables such as butternut and pumpkin. Although the different OFSP cultivars had similar characteristics, differences were found in the moist, adhesive and grainy texture attributes as well as the vegetable sweet flavour. Resisto had the sweetest in flavour, with the most dense and pasty texture of the four OFSP cultivars. W119 had the grainiest texture. No significant differences were found in the earthy aroma, sweet potato and yellow vegetable flavour attributes. It can be concluded that in flavour, few differences were found among the OFSP, except in sweetness. OFSP differed primarily from each other in texture. The consumer preference test results showed that overall 85% of respondents preferred the taste of OFSP to that of WFSP, 53% liked the orange colour a lot while 24% liked the colour a little and the remaining 22 % disliked the colour (either a lot, a little, neither like nor dislike). The majority of the consumers (86%) indicated a willingness to buy OFSP. From these results it can be concluded that the taste and colour of OFSP are acceptable to consumers of sweet potato and that it has potential to be successful in the marketplace. Given the high level of consumer acceptability of OFSP, an opportunity exists to address vitamin A deficiency through commercially viable decentralised vine production centres. Such centres could be managed by commercial-, small-scale- and subsistence farmers in South Africa in key sweet potato production areas, with adequate water supply throughout the year. However, availability of commercially produced OFSP in retail sores in recent months, may change this prospect. / Dissertation (MConsSci)--University of Pretoria, 2008. / Consumer Science / unrestricted

No description available.

Sweet clover plants become more susceptible to low temperatures as they advance in age. They are most resistant when in the crook stage. Plants resulting from unhulled seed are more vigorous and resistant to low temperatures than are plants resulting from scarified seed. Plants from unhulled seed produce larger bay yields and more profits than do similar plants resulting from scarified seed. Soils containing a medium percentage of moisture afford more optimum conditions for plant growth than do soils of extremely low or high moisture content. There is an indication, but not conclusive, that the physiological activity of seedlings from scarified seed is slightly more rapid than is the ease of imhulled seedlings. Alternate freezing and thawing of impermeable sweet clover seed, or treating with concentrated sulphuric acid, does not increase, to any significant degree, the percentage of germination. It seems that the subjection of seed to a moist condition is just as effective as any attempt to increase germination by artificial means. / M.S.

Since sweet potatoes, which also furnish vitamin A, are a common food in the popular diets of Texas and are so generally grown over the state, the purpose of this study is (1) to ascertain the amount of vitamin C in eight of the varieties most commonly grown and (2) to determine the effect of the three most popular methods of cooking (boiling, baking, and candying) on the vitamin C content of these varieties of sweet potatoes.

The purpose of this study was to determine by biological method of assay the vitamin A content of Puerto Rico sweet potatoes which were dehydrated immediately after harvesting.