Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 114-128). Also available on the Internet.

Raspberry pomace, consisting of seeds, pulp and added rice hulls, is the residue from the pressing of raspberries for juice and concentrate production. Through the determination of chemical composition by laboratory analyses and the measurement of feeding value in animal trials, the pomace was evaluated as a feedstuff for growing pigs. Pomace contains 11.1% crude fat, 10.0% crude protein, 59.5% total dietary fibre, 7.4% soluble carbohydrates, and a gross energy level of 5220 kcal*kg⁻¹. The acid detergent residue of the pomace contains 11.7% lignin, 6.0% cutin, 2.2% acid detergent ash and 26.0% cellulose (by difference). The digestibility of dry matter, fat, protein and energy was determined. Pomace dried at 60 C, whole and ground (1mm) was fed to growing male pigs (30-35 kg) in a replicated 4X4 Latin Square design with treatments basal (B) , B plus 40% unground pomace, B plus 40% ground pomace and B plus 40% barley. Grinding of pomace significantly improved the digestibility of all parameters measured, however, the barley-soybean meal basal ration was consistently better digested than either of the pomace treatments. The digestibility of whole and ground pomace was respectively: dry matter 10.7% and 20.8% (S.E.M. 1.30), fat 24.1% and 79.7% (S.E.M. 3.47), protein 10.6% and 14.7% (S.E.M. 4.83) and energy 7.9% and 28.4% (S.E.M. 1.80). Protein quality of ground (1mm) and freeze-dried pomace was evaluated with rats in metabolism cages to produce the following values: true protein digestibility 36.0% (S.E.M. 0.66), biological value 91.0% (S.E.M. 3.46), and net protein utilization 32.7% (S.E.M. 1.15). In rat growth trials, where pomace replaced barley incrementally, growth rate was not affected at replacement levels up to 40%, although feed efficiency declined consistently as the level of pomace in the diet increased. It is suggested that raspberry pomace could replace up to 20% of an energy feedstuff in a ration for growing swine without significantly reducing growth rate or feed efficiency. / Land and Food Systems, Faculty of / Graduate

Thesis (MPhil)--Stellenbosch University, 2004. / ENGLISH ABSTRACT: The primary objective of today's pig producer is to maximize profit received per unit of pig space provided in a given facility. There are certain management strategies that a farmer can implement to ensure this. The goals of such strategies include maximizing performance and implementing proper pig flow schedules and to make sure current facilities are performing at maximum capacities or are better utilized. Labour in South Africa is still relatively inexpensive, but time is money. Feeding and cleaning small pens are time consuming and expensive to maintain (more feeders and water nipples, gates, walls and heating). Proper management of facility space is vital to remain competitive in today's pig industry. The amount of floor and feeder space provided within an animal's environment could vastly influence pig performance and profitability. Crowding pigs has a negative effect on Average Daily Gain (ADG) and overall performance. Conversely, facility cost per pig increases as additional space is provided, therefore, in the interest of pen efficiency, the space that allows for maximum individual performance may not be optimal for maximizing profit. Factors such as cleaning and cleaning time also have an effect on profitability. Another concerning factor associated with maximizing performance and maintaining the pig flow of a facility is that of Body Weight (BW) variation. Having uniform pigs at 70 day, post-weaning has been a major goal to ensure maximum performance in finishers. Numerous contradictions regarding the growth of piglets post-weaning (especially the effect of group size and floor space allowance) still exist in literature. Also, in South Africa, the majority of pig producers still believe that individual litters or small groups of pigs in the growers perform better and are the most economical production method. Therefore, this trial was performed to investigate the raising of large groups (200-450 pigs/group) compared to small groups (10-20 pigs/group) on their performance until 70 days when they were moved to growers. Over a period of two years, data was collected on a commercial 1200 sow unit in the Western Cape, South Africa. Crossbred pigs (n=14657; Landrace x Large White; both gilts and barrows) weaned at 19 to 24 days with average weaning weight of 5.11 kg were randomly divided into different group sizes. Groups of 10, 20, 200 and 450 were grouped together and were all given the same feed ration. The groups were housed in different buildings naturally ventilated with no artificial heating and with solid flooring. Pelleted diets were formulated (Startrite followed after two weeks with Expresweaner) by Meadows Feed Mills (Paarl, Western Cape) for weaned piglets and fed to all the piglets. The animals received the diets and water ad lib. All the piglets were weighed before being placed into the different pens (initial weight). After 69 - 72 days the piglets were weighed again (final weight). The pigs were weighed as a group and not individually. The data was statistically analysed by determining averages and standard deviations for each house. The results of this trial clearly indicate that raising piglets in larger groups does not negatively affect the performance of the piglet in terms of ADG and mortality. In larger groups, the less time (labour time) spent cleaning and the fewer feeders needed, also results in lower production costs. In today's economical environment, the producer that can lower his production cost while maintaining production standards has a significant advantage. Therefore, it is believed that by grouping pigs in larger groups, labor time (in terms of cleaning) can be reduced, and will lower production cost without reducing production standards. / AFRIKAANSE OPSOMMING: Die hoofdoel van vandag se varkprodusente is om maksimum wins per eenheid vark spasie te maak. Daar is sekere produksietegnieke wat 'n produsent kan volg om dit te verseker. Die doel van die tegnieke is om te verseker dat maksimale produksienorme en doeltreffende vark vloei gehandhaaf word asook dat huidige fassiliteite optimum benut word. Arbeid is nog relatief goedkoop in Suid-Afrika (SA), maar tyd is geld. Die voeding en skoonmaak van klein hokke is tydrowend en duur om te onderhou (meer voerbakke, waternippels, hekke, mure en verhitting). Om koste-effektief te boer, moet goeie bestuur van oppervlakte toegepas word. Die hoeveelheid oppervlakte en voerspasie beskikbaar, het 'n groot invloed op die produksievermoë en winsgewendheid van die varkies. Om te veel varkies per area aan te hou, het 'n negatiewe effek op die Gemiddelde Daaglikse Toename (GOT) en algehele prestasie. Soos die vloerspasie per varkie vergroot, neem die produksiekoste ook toe en is die area wat benodig word om varkies maksimaal te laat produseer, nie altyd die winsgewenste nie. Skoonmaak en skoonmaaktyd het ook 'n invloed op winsgewendheid. Die ideaal is om uniforme varkies op 70 dae te produseer. 'n Variasie in gewig is rede tot kommer, aangesien dit die produksie in die groeiafdeling beïnvloed. Verskeie teenstrydighede aangaande die groei van varkies naspeen (veral die effek van goepgrootte en vloerspasie) bestaan steeds in die literatuur. In SA is die meeste produsente ook nog onder die indruk dat klein groepe varkies beter presteer as groot groepe en die mees ekonomiese metode is om te boer. Daarom is 'n proef geloods om vas te stel hoe die produksie verskil tussen klein groepe (10 - 20 varkies per groep) teenoor groot groepe (200 - 450 varkies per groep) tot 70 dae ouderdom wanneer hulle na die groeiafdeling skuif. Oor 'n periode van twee jaar is data gekollekteer op In kommersiële 1200 sog-eenheid in die Wes-Kaap, Suid-Afrika. Kruisgeteelde varke (n=14657; Landras x Groot Wit; beide beertjies en soggies) is gespeen tussen 19 en 24 dae, met 'n gemiddelde speen gewig van 5.11 kg en ewekansig verdeel in verskillende groepgroottes. Groepe van 10, 20, 200, en 450 varkies was saamgegroepeer en het almal dieselfde dieet ontvang. Die groepe is in verskillende geboue gehuisves en al die geboue het soliede vloere gehad en was natuurlik geventileerd met geen kunsmatige verhitting nie. 'n Verpilde dieet is deur Meadows Voermeule (Paarl, Wes-Kaap) geformuleer (Startrite vir twee weke en dan Expresweaner vir die res van die proef). Die varke het die dieet en water ad lib ontvang. AI die varkies is geweeg voordat hulle in die verskillende hokke geplaas is (aanvangsmassa). Na 69 - 72 dae is die varkies weer geweeg (eindmassa). Die varkies is as 'n goep en nie individueel geweeg nie. Die data is statisties verwerk deur gemiddeldes en standaard-afwykings vir elke huis te bepaal. Die resultate van hierdie proef het duidelik aangetoon dat die grootmaak van varkies in groter goepe nie die produksie van die varkies (i.t.v. GOT of mortaliteit) nadelig beïnvloed het nie. In groter goepe is minder tyd nodig om die hokke skoon te maak en is minder voerders nodig. Dit lei tot 'n verlaging in produksiekoste. Deesdae het die produsent wat sy produksie koste kan verlaag, sonder om produksiestandaarde in the boet, 'n aansienslike voorsprong. Die gevolgtrekking word dus gemaak dat deur varkies in groter groepe aan te hou, arbeid (in terme van skoonmaak) verminder kan word en dus kan produksiekoste verminder sonder dat produksie self afneem.

published_or_final_version / Zoology / Master / Master of Philosophy

Call number: LD2668 .T4 1959 R66

Call number: LD2668 .T4 1986 R52 / Master of Science / Animal Sciences and Industry

Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 105-113). Also available on the Internet.

This item was digitized as part of the Million Books Project led by Carnegie Mellon University and supported by grants from the National Science Foundation (NSF). Cornell University coordinated the participation of land-grant and agricultural libraries in providing historical agricultural information for the digitization project; the University of Arizona Libraries, the College of Agriculture and Life Sciences, and the Office of Arid Lands Studies collaborated in the selection and provision of material for the digitization project.

The objective of this study was to investigate whether or not the treatment of nuclear donor cells with inhibitors of protein synthesis and cyclin-dependent kinases (CDKs) affect the development of swine embryos produced by somatic cell nuclear transfer. Host oocytes were derived from pre-pubertal females and matured in vitro for 42-46 h under standard conditions. Nuclear donor cells were fetal fibroblasts maintained in culture for 2 to 6 passages. Oocytes were reconstructed with cells treated for 22-24 hours with cycloheximide (CHX; 10mug/ml), roscovitine (ROS; 25 muM), the combination of CHX + ROS (CR), or untreated cells. Two hours after reconstruction, the oocytes were activated using ionomycin (15muM/5 min) and strontium chloride (10mM/4h), maintained for 6 h in the presence of cytochalasin B (7.5mug/ml) and CHX (10mug/ml), and then cultured in porcine zygote medium (PZM3) for 6 days. The cleavage rate, 63.7% (n=318), 55.2% (n=99), 56.7% (n=107) and 60.6% (n=347), at 48 h post-fusion were not significantly different between embryos derived from ROS, CHX, CR and control cells, respectively. Developmental rate to blastocyst stage was higher for embryos reconstructed with ROS (12.2%) and untreated cells (12.1%) when compared to CHX (5.7%) and CR (4.9%). Blastocysts produced with ROS treated cells had similar number of nuclei compared to embryos reconstructed with untreated donor cells (30.9+/-10.4 vs. 32.2+/-8.0). Phosphorylated H2A.X (gammaH2A.X) was highly expressed in donor cells treated with CR compared to non treated cells, but it was similarly expressed in most of 1-cell stage embryos reconstructed with control or treated cells. Flow cytometry analysis showed that the majority of the fibroblasts were at G 0/G1 phase of the cell cycle at the time of nuclear transfer. It was concluded that the treatment of nuclear donor cells with inhibitors of protein synthesis and CDKs did not improve the in vitro development of somatic cell nuclear transfer embryos in pigs.

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