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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.

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Spelling suggestions: "subject:"t?cnicas dde purificar??o"" "subject:"t?cnicas dde purificada??o""

1

Utiliza??o da t?cnica de western blotting para diagn?stico da infec??o por Cystoisospora felis (Wenyon, 1923) Frenkel, 1977 (apicomplexa: Cystoisosporinae) em coelhos (Oryctolagus cuniculus) / The use of Western Blotting technique to determine Cystoisospora felis (Wenyon, 1923) Frenkel, 1977 (Apicomplexa: Cystoisosporinae) infection in rabbits (Oryctolagus cuniculus)

Meireles, Gisele Santos de 27 February 2009 (has links)
Made available in DSpace on 2016-04-28T20:15:32Z (GMT). No. of bitstreams: 1 2009 - Gisele Santos de Meireles.pdf: 1867896 bytes, checksum: 50898c1a65b34d41d9d4352322ce8d1e (MD5) Previous issue date: 2009-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This work aimed, to determine the phenotypic analysis from sporulated oocysts of Cystoisospora felis by using a sequential method of cleaning techniques. By using SDS-PAGE at 10 and 12 % were separated 22 protein groups as: 26.65; 29.71; 31.79; 36.41; 44.03; 50.09; 56.08; 62.64; 73.65; 77.55; 85.34; 97.62; 98.66; 101.24; 104.21; 109.23; 110.56; 113.21; 138.48; 180.50; 206.81 and 244.51KDa belonged to estrutucture from C. felis sporulated oocysts. Western Blotting technique was performed after SDS-PAGE and were identified these antigenic proteins: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 and p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa from hyperimmune and natural infected rabbits by C. Felis respectively. / Este trabalho teve por objetivo, tra?ar um perfil prot?ico de oocistos esporulados de Cystoisospora felis, recuperados a partir do uso seq?encial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 10 e 12 % resultou na identifica??o de 22 grupos prot?icos de 26; 29; 31; 36; 44; 50; 56; 62; 73; 77,55; 85,34; 97,62; 98,66; 101,24; 104,21; 109,23; 110,56; 113,21; 138,48; 180,50; 206,81 e 244,51 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de coelho heter?logo anti-C. felis foi poss?vel desenvolver uma t?cnica de diagn?stico imunoenzimatico com western Blotting para identifica??o de animais infectados de maneira natural ou experimental com C. felis, identificando os seguintes prote?nas antig?nicas: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 e p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa, respectivamente.
Cystoisospora felis
t?cnicas de purifica??o
SDS-PAGE
Western Blotting.
western blotting
purification techniques

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