Made available in DSpace on 2016-04-28T20:15:22Z (GMT). No. of bitstreams: 1 Janaina Soledad Rodrigues-pdf.pdf: 4634356 bytes, checksum: 26f90dcd5b3f127e6130fc4b467f87d1 (MD5) Previous issue date: 2010-02-24 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Cystoisospora felis is an obligatory intracellular parasite that infecting felines of diverse species, that were contaminated by ingesting sporulated oocysts end/or intermediated host harboring monozoic cysts of this species. In this study, 10 Japanese quails were infected with a pure inoculum consisted by 106 sporulated oocysts of C. felis, after 60 days post infection, liver, spleen, and bursa of Fabricius were removed from each quail and they were given to feed to 3 kittens separately. A forth kitten was infected with 106 sporulated oocysts orally. After shedding oocysts by kittens, they were allowed to sporulate. After that, 50 sporulated oocysts of each infection were measured, having as means of Length, Width and Shape Index the following values: 44.30?2.30, 31.30?1.90 ?m and 1.40?0.1 from kitten fed on liver, 46.30?2.20, 32.90?1.90 ?m and 1.40?0.1 from kitten fed on spleen, 44.80?2.20, 31.20?1.60 ?m and 1.40?0.1 from kitten fed on bursa of Fabricius, and 43.60?1.60, 30.80?1.60 ?m and 1.40?0.1 from kitten infected with sporulated oocysts orally as well as C. felis prepatent and patent periods. By analyzing the frequency of the distribution of values was observed that when the source of infection were liver and spleen, the distribution was more homogeneous, which did not occur when was used cloacal bursa or sporulated oocysts as sources of infection. As conclusions in this work, there were no significant differences among de morphometry of the sporulated oocysts, independent of the source of infection. Moreover, the pre-patent and patent were similar when cats fed on infected viscera of quails experimentally infected with sporulated oocysts of C. felis previously when compared with the animal that received 1x106 sporulated oocysts orally. / Cystoisospora felis ? um parasito intracelular obrigat?rio que acomete fel?deos de diversas esp?cies, que podem se contaminar tanto pela ingest?o de oocistos esporulados, quanto pela ingest?o de tecidos de hospedeiros intermedi?rios infectados previamente com oocistos esporulados. Neste estudo, 10 codornas japonesas foram infectadas com um inoculo puro de oocistos esporulados de C. felis na concentra??o de 1 x 106, e ap?s 60 dias, ba?o, f?gado e bursa dessas codornas infectadas foram oferecidos a tr?s filhotes de gato livres de infec??o. Um quarto gato ainda foi infectado com uma suspens?o de 1 x 106 oocistos esporulados de C. felis por via oral. Ap?s o in?cio da elimina??o, os oocistos foram colocados para esporular e, ap?s a esporula??o, 50 oocistos oriundos de cada infec??o foram mensurados, tendo como m?dias de di?metros maior (DM), menor (dm) e ?ndice morfom?trico os seguintes valores: 44,30?2,30, 31,30?1,90 ?m e 1,40?0,1 para os oriundos do animal infectado com f?gado, 46,30?2,20, 32,90?1,90 ?m e 1,40?0,1 para os oocistos do animal infectado com ba?o, 44,80?2,20, 31,20?1,60 ?m e 1,40?0,1 para os oocistos oriundos do animal infectado com bursa e 43,60?1,60, 30,80?1,60 ?m e 1,40?0,1 para os oocistos do animal que recebeu oocistos por via oral. Ao analisar a distribui??o da frequ?ncia dos valores dessas medidas, pude-se observar que quando a fonte de infec??o utilizada foi f?gado e ba?o, a distribui??o foi mais homog?nea, o que n?o ocorreu quando utilizou-se bursa cloacal ou oocistos esporulados como fonte de infec??o. Com estes resultados foi poss?vel concluir que n?o se observaram diferen?as significativas na morfometria dos oocistos de C. felis independente da fonte de infec??o. Al?m disso, os per?odos pr?-patente e patente foram semelhantes quando os gatos livres de infec??o foram alimentados com v?sceras de codornas, infectadas previamente com oocistos esporulados de C. felis em compara??o com o animal que recebeu 1 x 106 oocistos esporulados por via oral.

Made available in DSpace on 2016-04-28T20:15:32Z (GMT). No. of bitstreams: 1 2009 - Gisele Santos de Meireles.pdf: 1867896 bytes, checksum: 50898c1a65b34d41d9d4352322ce8d1e (MD5) Previous issue date: 2009-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This work aimed, to determine the phenotypic analysis from sporulated oocysts of Cystoisospora felis by using a sequential method of cleaning techniques. By using SDS-PAGE at 10 and 12 % were separated 22 protein groups as: 26.65; 29.71; 31.79; 36.41; 44.03; 50.09; 56.08; 62.64; 73.65; 77.55; 85.34; 97.62; 98.66; 101.24; 104.21; 109.23; 110.56; 113.21; 138.48; 180.50; 206.81 and 244.51KDa belonged to estrutucture from C. felis sporulated oocysts. Western Blotting technique was performed after SDS-PAGE and were identified these antigenic proteins: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 and p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa from hyperimmune and natural infected rabbits by C. Felis respectively. / Este trabalho teve por objetivo, tra?ar um perfil prot?ico de oocistos esporulados de Cystoisospora felis, recuperados a partir do uso seq?encial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 10 e 12 % resultou na identifica??o de 22 grupos prot?icos de 26; 29; 31; 36; 44; 50; 56; 62; 73; 77,55; 85,34; 97,62; 98,66; 101,24; 104,21; 109,23; 110,56; 113,21; 138,48; 180,50; 206,81 e 244,51 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de coelho heter?logo anti-C. felis foi poss?vel desenvolver uma t?cnica de diagn?stico imunoenzimatico com western Blotting para identifica??o de animais infectados de maneira natural ou experimental com C. felis, identificando os seguintes prote?nas antig?nicas: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 e p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa, respectivamente.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-10-24T18:46:15Z No. of bitstreams: 1 2013 - Gisele Santos de Meireles.pdf: 16741114 bytes, checksum: 1d4b03cbd2287a0984257f70efb149da (MD5) / Made available in DSpace on 2018-10-24T18:46:15Z (GMT). No. of bitstreams: 1 2013 - Gisele Santos de Meireles.pdf: 16741114 bytes, checksum: 1d4b03cbd2287a0984257f70efb149da (MD5) Previous issue date: 2013-02-27 / CAPES / FAPERJ / This study aimed to determine from the protein profile of oocysts of Cystoisospora felis recovered from the sequential use of various purification techniques adapted for specific use in sporulated oocysts of C. felis. With the aid of SDS-PAGE 12% resulted in identification of 25 groups of protein: 266, 240, 186, 165, 140, 119, 112, 105, 98, 90, 78, 55, 47, 42, 37, 35, 30, 27-28, 25, 22, 19, 18, 16, 14 kDa belonging to the structure of sporulated oocysts and sporozoites of C. felis. Based on this results and heterologous bovine serum anti-C. felis was possible to determine polypeptides dominant relevant to diagnostic immunoassay technique with "Western Blotting", these being immunodominant bands: P208, P138, P113, p106, p62, p56, p51, p48, p44, p38, p36, and p33 p27. In order to avoid misdiagnosis from cross-reactivity a positive control serum anti-C. felis was compared to with positive serum anti-Toxoplasma and Neospora in order to exclude the common protein bands, probably markers of gender and group to identify cattle infected naturally or experimentally with C. felis. As showed, the following specific antigenic protein units: p 206-208, P137-139, p112- 113, p104-107, p27-28 are responsible for determining the animal tested positive or not for Cystoisospora felis. Of the analysis of the variables could be observed that the presence of felines related to the handling, size and type of milking properties facilitates dispersion C. felis. / Este trabalho teve por objetivo, diagnosticar a infec??o por Cystoisospora felis em bovinos atrav?s do Western Blotting, apartir de oocistos obtidos com o uso sequencial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 12 % resultou na identifica??o de 25 grupos proteicos de: 266; 240; 186; 165; 140; 119, 112, 105, 98, 90, 78, 55, 47, 42, 37, 35, 30, 27-28, 25, 22, 19, 18, 16, 14 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de bovino heter?logo anti-C. felis foi poss?vel determinar os polipept?deos dominantes relevantes ? t?cnica de diagn?stico imunoenzimatico com ?Western Blotting?, sendo estas, as bandas imunodominantes: p208, p138, p113, p106, p62, p56, p51, p48, p44, p38, p36, p33 e p27. A fim de evitar o diagn?stico equivocado a partir de rea??es cruzadas foi feita a compara??o do soro controle positivo anti-C. felis com o soro positivo anti-Toxoplasma e Neospora com o intuito de excluir as bandas proteicas comuns, prov?veis marcadoras de g?nero e grupo para identifica??o de bovinos infectados de maneira natural ou experimental com C. felis. Sendo evidenciadas, as seguintes unidades proteicas antig?nicas espec?ficas: p 206-208, p137-139, p112-113, p104-107, p27-28 respons?veis por determinar a positividade dos animais testados para C. felis. A partir das an?lises das vari?veis foi poss?vel observar que a presen?a de felinos associados, ao manejo, tipo de ordenha e tamanho das propriedades facilita a dispers?o de C. felis.